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Citations for Millipore Sigma :
2451 - 2500 of 4463 citations for Recombinant Human IL12B Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: Expression of recombinant extracellular proteins was performed by transient transfection in HEK−293F cells using polyethylenimine (PEI, Sigma Aldrich). 300 mL of culture at a density of 1.0 × 106cells/mL was transfected with 300 μg DNA using 450 μg PEI ...
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bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl) in a 1:3 volume ratio for recombinant enterokinase digestion (Novagen, 1 unit/ 50 μg protein) overnight (Gasparian et al. ...
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bioRxiv - Immunology 2023Quote: ... SRBCs in Alsever’s (TCS Bioscience) were conjugated to recombinant HEL3X (R. Brink) with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Sigma) as previously described104 ...
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bioRxiv - Cell Biology 2023Quote: ... imidazole 5 mM and recombinant RAD51-proteins were purified on a NiNTA resin (#P6611, Sigma, St Louis, MO, USA) using the washing buffer ...
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bioRxiv - Neuroscience 2023Quote: Primary rat cortical cultures were treated with recombinant Tat 1-86 (Diatheva, Italy) and with cocaine-HCl (Sigma Chemicals) as described in each Figure Legend ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing recombinant PKAc verified via SDS-PAGE were pooled and concentrated to 250μL using an Amicon concentrator (10K MWCO; Millipore). The concentrated sample was then applied to a preparative-grade Superdex200 column (GE Healthcare ...
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bioRxiv - Plant Biology 2023Quote: 6xHistidine-Glutathione-S-transferase (GST) tagged proteins of the predicted intracellular region of SOBIR1 (termed the KD) was cloned into pCDF-Duet vector (Novagen, EMD Chemicals, San Diego, CA) and expressed in E ...
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bioRxiv - Developmental Biology 2021Quote: ... The proximal forelimbs of embryos were dissected (see Figure 3) and placed into PBS with 10% FCS and 8ul collagenase at 50mg/ml (Sigma C9697) at 37° for approximately 5min ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with 3-isobutylmethyl-xanthine (1 mM)-containing medium or including the secondary antibody anti-mouse IgG (Fc specific, M2650, Sigma-Aldrich) for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... (ATCC) cells were maintained in DF-1 cell culture media (DMEM supplemented with 10% FCS, 5% tryptose phosphate broth (Sigma-Aldrich), 1% NEAAs and 1% penicillin-streptomycin and maintained at 39 °C in a 5% CO2 atmosphere.
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bioRxiv - Cell Biology 2019Quote: ... purified DN3a cells were cultured on OP9-DL1 stromal cells or Fc-DL1 functionalised surfaces in the presence of AMD3100 (2 μg/ml, Sigma-Aldrich) or DBZ (0.05 µM ...
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bioRxiv - Genetics 2021Quote: ... EDL muscles were digested in Krebs solution (without FCS) containing 3 mg/ml collagenase type IV A (Sigma Aldrich, MO, USA), gently bubbled with carbogen (95% O2 ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10 % Foetal Calf Serum (FCS, LabTech International Ltd, Heathfield, East Sussex, UK) and 4 mM L-glutamine (Sigma-Aldrich).
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bioRxiv - Biochemistry 2020Quote: ... and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143, Sigma Aldrich, USA; 30 min at 4°C).
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bioRxiv - Neuroscience 2021Quote: 4% PFA-fixed brains were washed twice in 20 mL PBS for a total of 24 hours and imaged in Fluorinert FC-40 (Sigma Aldrich) for null background signal ...
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bioRxiv - Bioengineering 2021Quote: Each intact piglet brain hemisphere was placed in a 30 mm diameter glass tube containing perfluorocarbon oil (Fluorinert FC-40, Sigma-Aldrich) as a magnetic susceptibility matching fluid ...
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bioRxiv - Microbiology 2021Quote: ... gondii parasites were cultured in complete medium (DMEM, +5 % FCS) or in custom culture medium −Pan (DMEM −Pan, +5 % dialyzed FBS) supplemented with Val (Sigma, V6504), pantoate (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Thymic fragments were washed in RPMI 2% FCS and digested 15 min at 37 °C in 500 μg/mL Liberase TL (Sigma-Aldrich) and 111 μg/mL DNase I from bovine pancreas (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2020Quote: ... in 20 ml Dulbecco’s Modified Eagle Medium (DMEM, Capricorn, Ebsdorfergrund, Germany) containing 10 % fetal calf serum (FCS, Sigma, St. Louis, USA) and 1 % penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2022Quote: ... Any larger tissue fragments were dissociated mechanically followed by washing of the cell strainer with MACS buffer (PBS, 2% FCS, 2mM EDTA (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2021Quote: ... Wells were washed 3 times with washing buffer then 100 μL of diluted goat anti-mouse IgG (Fc specific)-Peroxidase antibody (#A0168; Sigma-aldrich) were added ...
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bioRxiv - Microbiology 2022Quote: ... Plates were washed 4 times with PBS/Tween and then incubated for 1 h at RT with 100 μL/well of an anti-mouse Fc conjugated to alkaline phosphatase (Sigma-Aldrich), diluted 1:1000 in blocking buffer ...
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bioRxiv - Immunology 2022Quote: Each SMG pair (approx. 160mg) was mechanically minced in 2mL of RPMI-1640 containing 5% fetal calf serum (FCS; Sigma Aldrich), 25µl Collagenase-II (23mg/mL ...
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bioRxiv - Immunology 2019Quote: ... Cells were plated at 0.5-1 × 105 cells in a 96 well plate in L-15 media containing 5% FCS and exposed to DMSO (vehicle control) or 50 ug/ml 17-β estradiol or Bis-phenol A (Sigma) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Afterwards the medium was switched and cells were cultivated for 4 days in DMEM containing 10% FCS and 2 μM insulin (Sigma Aldrich) to induce adipogenic differentiation ...
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bioRxiv - Pathology 2020Quote: SFs were seeded in 6-well plates at 1×106 cells per well and synchronised overnight in 1% FCS DMEM before being treated for 24 hours with the indicated stimuli: LPS (1 µg/ml, Sigma-Aldrich), IL-1β (10ng/ml ...
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bioRxiv - Cell Biology 2021Quote: ... [42] After expansion in complete A-RPMI, pUC were cryopreserved in heat inactivated fetal calf serum (FCS, Biochrom) supplemented with 10 % Dimethyl sulfoxide (DMSO, Sigma Aldrich) until use ...
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bioRxiv - Immunology 2020Quote: ... cells were expanded in selection medium (10 % dialysed FCS, 1x GSEM supplement [Sigma-Aldrich], 20–50 μM MSX, 1 % Pen/Strep) for at least 1 week ...
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bioRxiv - Immunology 2021Quote: ... cells were expanded in selection medium (10 % dialysed FCS, 1x GSEM supplement [Sigma-Aldrich], 20–50 µM MSX, 1 % Pen/Strep) for at least 1 week ...
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bioRxiv - Immunology 2021Quote: ... 721.221 was purchased from the International Histocompatibility Working Group and grown in RPMI-1640 supplemented with 15% heat-inactivated fetal calf serum (FCS) (Sigma-Aldrich), sodium pyruvate (Thermo Fisher) ...
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bioRxiv - Immunology 2020Quote: Urine cells were thawed into 9 ml of 10% FCS-RPMI medium supplemented with 10 IU/ml Benzonase (Novagen 70664-3), counted and viability determined by trypan blue exclusion ...
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bioRxiv - Immunology 2019Quote: Small intestinal biopsies were collected at the time of endoscopy and transported in R10 (RPMI-1640 [Lonza] + 10% FCS [Sigma-Aldrich] + 1% penicillin/streptomycin [Sigma-Aldrich]) ...
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bioRxiv - Cell Biology 2020Quote: ... OMP-18R5 (OncoMed Pharmaceuticals, Redwood City, CA) at 10μg/mL with antihuman IgG (Fc) secondary conjugated to FITC (Millipore Sigma AP113F).
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bioRxiv - Immunology 2020Quote: ... Freshly isolated PBMCs from four donor M1/M2 MCM were pooled and resuspended in RPMI-1640 containing 10% fetal calf serum (FCS; R10) and 5 ug/ml phytohemagglutinin-P (Sigma-Aldrich). The culture was incubated overnight at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Immunology 2020Quote: ... and 2.5 ng/mL TNF-α for 3-5 days and subsequent differentiation in RPMI (Sigma, St Louis, Mo; +10% FCS) either with 100 ng/mL GM-CSF ...
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bioRxiv - Immunology 2022Quote: ... fibroblasts and MCF 10A cells were incubated with the FITC bound anti-mouse IgG secondary immunoglobulin (Fc specific) (Cat # F4143, Sigma-Aldrich) secondary antibody for 2 h at room temperature away from light ...
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bioRxiv - Cell Biology 2022Quote: ... A HeLa cell line stably expressing connexin43 [47] grown in DMEM/10%FCS/P/S supplemented with 1 μg/ml puromycin (Sigma #P9620) were solubilized in 1% Chaps/TBS/5% glycerol at pH 7.4 supplemented with protease blockers ...
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bioRxiv - Synthetic Biology 2022Quote: ... Single-cell suspensions were derived by mechanical disruption of bone marrow in PBS supplemented with 2% Fetal Calf Serum (FCS, Sigma, C8056). For FACS analysis ...
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bioRxiv - Microbiology 2022Quote: ... and suspended at 2 x 104 cells/mL in Titan Cell Medium (TCM) composed by 5% Sabouraud, 5% inactivated Fetal Calf Serum (FCS, Biological Industries) and 15 µM sodium azide (Sigma Aldrich), diluted in 50 mM MOPS adjusted to pH 7.3 ...
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bioRxiv - Microbiology 2022Quote: MPXV strains were diluted ½ in two different kinds of media that is Viral Transport Media (VTM; 330C, Copan) and FCS (F2442, Sigma-Aldrich). Two hundreds microliters of each sample were submitted in triplicate to various temperatures for different periods of time ...
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bioRxiv - Microbiology 2024Quote: ... BEAS-2B cells grown in 6-wells plates were first transduced with lentiviruses encoding Cas9 diluted in infection medium (DMEM, 2% FCS) containing 8 μg/ml Polybrene (Sigma Aldrich). At 16 h post transduction ...
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bioRxiv - Microbiology 2023Quote: ... Isolates were standardised to 1×106 cells/mL in Roswell Park Memorial Institute (RPMI) media supplemented with 5% (v/v) foetal calf serum (FCS [Sigma-Aldrich]) to simulate organic load ...
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bioRxiv - Molecular Biology 2024Quote: ... Negative selection was therefore carried out by culturing the parasites in the presence of 5-fluorocytosine (5-FC, Sigma-Aldrich, UK) at 48 mM and 2.5 nM WR99210 for a week ...
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bioRxiv - Immunology 2024Quote: ... sections were washed with PBS to remove OCT compound on the slides, and blocked with solutions containing imaging buffer (2% FCS, 0.1% Triton X-100 (Sigma, Cat. X100), 0.01% sodium azide) ...
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bioRxiv - Immunology 2023Quote: ... guts were transferred to a new 50 mL tubes containing 1x digestion mix (RPMI GlutaMAX™ containing 2% FCS, 1mg/mL of Colagenase VIII [Sigma] and 40 u/mL of DNAse I [Sigma]) and incubated for 20 min at 37 °C sin an orbital shaker at 200 rpm ...
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bioRxiv - Immunology 2023Quote: ... before counting in complete RMPI (cRPMI = naRPMI supplemented with 10% heat-inactivated FCS [#S-FBS-EU-015, Serana, Pessin, Germany], 25 nM β-mercaptoethanol [#M6250, Sigma] ...
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bioRxiv - Genetics 2023Quote: ... the tissue slurry was seeded directly on Matrigel coated dishes (45 μg/cm2) in DMEM/F12 containing 20%FCS (Sigma-Aldrich) and 10% horse serum (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... under agitation at room temperature for 20 min and re-probed with HRP-conjugated poly-clonal goat anti-mouse IgG Fc (1:5000 dilution, Sigma–Aldrich) for checking the integrity of the mouse IgG2b Fc domain of the fusion protein ...
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bioRxiv - Microbiology 2023Quote: ... ∼8 x 106 parasites were pelleted by centrifugation at 800 rcf for 7 min and resuspended in FCS-free HMI-9 media with 1 µg/mL Hoechst 33342 (Sigma-Aldrich). Cells were pelleted again ...
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bioRxiv - Immunology 2023Quote: ... Cells were re-suspended at 1×107 cells/ml in RPMI 1640 buffer supplemented with 10% foetal calf serum (FCS, Sigma-Aldrich) for further analysis ...