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Citations for Millipore Sigma :
2501 - 2550 of 10000+ citations for NKX2 4 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... log phase cells (OD600~0.5 - 0.6) with bar1Δ were arrested in late G1 phase using 50 ng/ml alpha-factor peptide (Sigma) for ~120 minutes ...
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bioRxiv - Biophysics 2021Quote: ... Target proteins were eluted with 5 column volumes of wash buffer plus 200 μg/mL FLAG peptide (Sigma). The eluent was then applied to Strep-Tactin Sepharose (IBA ...
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bioRxiv - Molecular Biology 2022Quote: ... Bound peptides were eluted in three steps with a solution of 2 mg/ml Oxone (Potassium peroxymonosulfate, Sigma) in H2O ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were extracted using solutions of 50% and 80% acetonitrile (ACN) with 0.5% formic acid (Millipore), and the recovered solution dried down in a vacuum concentrator ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... and then incubated with 3 resin volumes of 200 µg·ml-1 3xFLAG peptide (Sigma, catalog # F4799, lot # SLCJ4916) for 45 min to elute proteasome complexes ...
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bioRxiv - Molecular Biology 2024Quote: ... the beads were resuspended in 35 µL of TBS buffer (50 mM Tris-HCl, 150 mM NaCl, pH 7.5) supplemented with 0.5 µg/µL FLAG peptide (Sigma) and incubated at 4 °C for 15 min ...
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bioRxiv - Biochemistry 2022Quote: ... GB1-TAZ2 binding to MITF peptides was determined utilising a polyhistidine monoclonal mouse antibody (1:5000, Sigma, H1029), incubated on the array for 4 hours at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluates containing digested peptides were separated from beads by centrifugation through a 0.45 µM PVDF filter column (Millipore) and cysteines were subsequently reduced and alkylated by addition of 5 mM TCEP and 10 mM CAA for 30 min at 30°C ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins bound to the Flag antibodies were eluted using 400 µg/mL of 3xFlag peptide (Sigma, F4799) during 30 min at 4°C under agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides desalting was carried out using ZipTip U-C 18 spin columns (Cat. No. ZTC18M096, Millipore Corporation U.S.A.). The peptides were dried in a speed vac and then reconstituted with 0.1%Formic acid in water.
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bioRxiv - Molecular Biology 2023Quote: ... high pH reversed-phase peptide prefractionation was performed with sulfonated divinylbenzene (CDS Empore™ SDB-RPS, Sigma-Aldrich) using a step gradient of increasing acetonitrile concentrations (0-60% ACN ...
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bioRxiv - Molecular Biology 2023Quote: ... and subjected to binding of FLAG-tagged peptide species with anti-FLAG M2 magnetic beads (Sigma, cat # M8823). The purified FLAG-tagged peptides were harvested by eluting from the anti-FLAG M2 magnetic beads with 100 ng/µl 3xFLAG peptides.
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bioRxiv - Cell Biology 2024Quote: ... Beads were extensively washed in lysis buffer and elution was carried out with 3×FLAG peptide (Sigma-Aldrich), HA peptide (Roche) ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM DTT) before elution using wash buffer supplemented with 0.25 mg/ml 3xFLAG peptide (F4799, Sigma-Aldrich). Purified telomerase complex was verified by western blotting.
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bioRxiv - Cell Biology 2023Quote: ... Peptides contained a C-term PEG-Biotin and were synthesized using a preloaded PEG-Biotin resin (Sigma-Aldrich). Peptides were cleaved from the resin using TFA / Water / Thioanisole / Triisopropylsilane / Phenol / Ethanedithiol – 82.5/0.5/0.5/0.25/0.25/0.25 and precipitated into cold diethyl ether ...
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bioRxiv - Biochemistry 2023Quote: ... Core complexes were eluted from anti-Flag resin with Flag buffer containing 250 μg/ml 3xFlag peptide (Sigma). Fractions containing protein were pooled and loaded on a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... beads were resuspended in 35 µL TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.5) with 0.5 µg/µL FLAG peptide (Sigma) and incubated at room temperature for 15 min at 900 rpm ...
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bioRxiv - Biophysics 2024Quote: ... The Aβ (1–42) peptide (Stratech, A-1163, 0.5 mg) was initially dissolved in 100% HFIP (Sigma-Aldrich) at a concentration of μM ...
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bioRxiv - Microbiology 2024Quote: ... Stock solutions (1 mg/ml) were prepared by dissolving the peptides in 0.1% trifluoroacetic acid (TFA) (Sigma, Germany). The mass correctness and absence of degradation of the synthetic peptides were verified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS ...
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bioRxiv - Cell Biology 2024Quote: ... Elution was done by adding either 20 μl of 0.2 mg/ml of 3xFLAG peptide (Sigma-Aldrich F4799) or 20 μl of 1x Strep-Tactin Elution Buffer (IBA 2-1042-025 ...
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bioRxiv - Microbiology 2024Quote: Fifteen amino acid (aa) peptides overlapping 12 aa spanning the structural proteins of CHIKV were purchased from Sigma and were divided into 4 overlapping peptide pools ...
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bioRxiv - Neuroscience 2021Quote: Head samples were fixed with 4% paraformaldehyde in 0.1 mol/L phosphate buffer (4% PFA, Sigma-Aldrich), pH 7.3 overnight at 4°C ...
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bioRxiv - Genomics 2019Quote: ... Senescence was induced by addition of 400 nM 4-hydroxytamoxifen (4-OHT, Sigma Cat no. H7904-5MG) to the culture medium and samples were collected and processed at the time points indicated in the main text ...
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bioRxiv - Plant Biology 2019Quote: ... 0.1 % Triton X-100 and 1 mM 4-methylumbelliferyl-β-D-glucuronide (4-MUG; 69602, Sigma-Aldrich) at 37°C for 90 min ...
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bioRxiv - Neuroscience 2021Quote: ... post fixed in 4% PFA overnight at 4°C and subsequently embedded in 10% gelatin (Sigma Aldrich) dissolved in water for vibratome sectioning ...
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bioRxiv - Plant Biology 2021Quote: ... the GFP signal in the 4-days old etiolated seedlings was fixed in 4% paraformaldehyde (PFA, Sigma) overnight ...
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bioRxiv - Biophysics 2020Quote: ... The cells were collected by centrifugation (6000 g, 5 min, 4 °C, Sigma 4-16KS tabletop centrifuge), lysed in lysis buffer (5 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed 4 times with PBS-T and 4-NitroPhenyl phosphate (pNPP, Sigma-Aldrich, 71768) substrate added ...
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bioRxiv - Cell Biology 2019Quote: ... resuspended in the same buffer (4 ml each) containing HRV 3C protease (4 units/ml final; Novagen), and incubated without agitation (4°C ...
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bioRxiv - Physiology 2020Quote: ... The mice received 4-hydroxy tamoxifen (4-OHT) (10 mg/kg; Sigma-Aldrich, St. Louis, MO, USA) intraperitoneal (i.p. ...
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bioRxiv - Immunology 2019Quote: ... The aliquots were incubated at 4°C for 30 minutes with 200µL HBSS with 4% NCS (Sigma), 200µL of anti-CD16 beads ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were first manually decorated at 4 hpf and then treated with 4-hydroxytamoxiphen (Sigma cat 94873) between 6-8 hpf in the absence of light ...
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bioRxiv - Cancer Biology 2021Quote: ... supernatant was aspirated and fresh DMEM-F12 medium containing 4-hydroxytamoxifen (4-OHT, 100 nM, Sigma, #H7904) or equivalent amount of DMSO (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed 4 times with PBS-T and 4-NitroPhenyl phosphate (pNPP, Sigma-Aldrich, 71768) substrate was added ...
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bioRxiv - Microbiology 2021Quote: ... Immediately prior to plating on agar plates containing 20 mM 4-chloro-phenylalanine (4-CP; Sigma-Aldrich), the cells were washed twice with phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed 4 times with PBS-T and 4-NitroPhenyl phosphate (pNPP, Sigma-Aldrich, 71768) substrate was added ...
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bioRxiv - Molecular Biology 2020Quote: ... BMMCs were treated with 25 nM 4-hydroxytamoxifen (4-OHT; H7904-5MG, Sigma-Aldrich, St. Louis, MO) for five days as described previously10 ...
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bioRxiv - Biophysics 2022Quote: ... We dissolve 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium chloride (DMTMM) powder (Sigma Aldrich) in HEPES buffer (pH 7.18 ...
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bioRxiv - Developmental Biology 2022Quote: Jaw tissues were fixed with 4% PFA overnight at 4°C followed by 30% sucrose (Sigma, #RDD023) treatment until the tissue sank to the bottom of the tube ...
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bioRxiv - Immunology 2022Quote: ... spleens were fixed for 2 h at 4°C in 2 mL of 4% paraformaldehyde (Millipore Sigma) followed by an overnight incubation at 4°C in 2 mL of 30% sucrose (Millipore Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 4-(4-Diethylaminostyryl-1-methyl-pyridinium-iodid (DiAsp) and NPS-2143 hydrochloride were purchased from Sigma-Aldrich and were kept at −20 °C at a stock concentration of 50 mM in DMSO.
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bioRxiv - Neuroscience 2022Quote: ... followed by a mixture of both 4-Aminopyridine (4-AP, 100 µM, Sigma-Aldrich, Cat. No. 275875) and TTX was carried out to test whether laser-evoked events reflected monosynaptic inputs (Petreanu et al. ...
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bioRxiv - Microbiology 2023Quote: ... 20 μL of 4 mM 5-bromo-4-chloro-3-indolyl α-D-N-acetylneuraminic acid (Sigma) was added ...
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bioRxiv - Molecular Biology 2023Quote: ... and the coupling reagent 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM) chloride (Sigma-Aldrich). Stock solutions were prepared in phosphate-buffered saline at concentrations of 140 mM (PDH ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated in PTU water containing 10 μM 4-hydroxy tamoxifen (4-OHT) (Sigma Aldrich, H7904) from 30 to 52 hpf (Fig ...
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bioRxiv - Cell Biology 2024Quote: ... RAS translocation to the nucleus was induced by addition of 100 nM 4-hydroxytamoxifen (4-OHT) (Sigma). 4-OHT-containing medium was changed every 3 days.
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bioRxiv - Microbiology 2024Quote: ... CPSs were incubated with 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM, Sigma-Aldrich). The mixture was then ultrafiltered to remove extra DMTMM and mixed with carboxyl latex beads (2 μm ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated with primary antibodies (Table 3) diluted in Intercept (TBS) blocking buffer with 0.05% Tween-20 (Sigma-Aldrich) overnight at 4°C on an orbital shaker.
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked with either Odyssey Blocking Buffer (Licor Cat #927-40000) or 5% skimmed milk in TBST buffer (SIGMA) and incubated for 1h at room temperature or overnight at 4° ...
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bioRxiv - Cell Biology 2020Quote: ... These slides were rehydrated in 1x PBS at room temperature for 15 min followed by blocking for 1 hour in 1x PBS containing 3% BSA (Bovine serum albumin, Sigma). The slides were then incubated for 1 hour at room temperature with 1xPBS+3% BSA with respective antibodies ...