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Citations for Millipore Sigma :
2451 - 2500 of 10000+ citations for NKX2 4 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Tissues were incubated with blocking solution (0.5% Triton X, 2.5% bovine serum albumin [Sigma-Aldrich], 2.5% donkey serum [Sigma-Aldrich]) for one day at 37 °C before being incubated with the primary antibody at 1:500 for 3 days at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... blocking solution was removed and the sample was incubated in anti-fibronectin I antibody from rabbit (Sigma-Aldrich, #F3648), diluted 1:500 in blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... in samples where biotin-streptavidin amplification was used before blocking with 10% chemiblocker (Merck Millipore, Billerica, MA, USA, #2170) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections for immunohistological staining were incubated in a blocking solution consisting of 10% Bovine Serum Albumin (BSA, Sigma-Aldrich) in PBST (1X PBS + 0.3% Triton-X100 (Roth)) ...
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bioRxiv - Bioengineering 2023Quote: ... slides were incubated in a blocking solution containing 0.3% Triton X-100 and 10% normal donkey serum (EMD Millipore) in 1xPBS + 100 mM CaCl2 + 100 mM MgCl for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG and AgRP primary antibodies were used and diluted in blocking buffer at 1:200 (mouse anti-FLAG, Sigma) and 1:1000 (rabbit anti-AgRP ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed before blocking in PBS +0.01% Triton X-100 and 0.2% fish skin gelatin (Sigma-Aldrich - G7765) for 1 h at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... The embryos were rinsed and permeabilized in PBS-0.1% triton, 3 times 30 min, and incubated in blocking solution (PBS-0.1% triton, 1% donkey serum (D9663, Sigma)) prior to incubating with primary and secondary antibodies ...
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bioRxiv - Immunology 2024Quote: ... Non-specific binding in cells was prevented by incubating with blocking buffer (5% gelatin from bovine skin [Sigma Aldrich]) for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... paraformaldehyde in PBS and incubated for 30 min in blocking buffer containing 0.3% Triton X100 and 3% BSA (Sigma) in PBS to permeabilize the cells and block nonspecific staining ...
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bioRxiv - Cancer Biology 2024Quote: ... The fixed and permeabilized cells were then exposed to a blocking solution (3% bovine serum albumin (#A7906, Sigma‒Aldrich) in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Retinal tissues were first incubated with blocking solution (5% normal donkey serum, 0.5% Triton-X100 in PBS, Sigma-Aldrich) for 2 hours at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were in blocking buffer (5.0% rabbit serum (Labtech) + 0.1% Triton TM X-100 (Sigma-Aldrich, Dorset, UK) in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... Plates were washed three times at each step with wash buffer (PBS + 0.05% Tween-20 (PBS-T)) Wells were blocked with blocking buffer (PBS-T with 5% skim milk (Sigma)) for 1h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections or cells were pre-incubated in blocking buffer containing 2–10% normal donkey serum (Merck Millipore, Burlington, MA) and 0.1% Triton X-100 at RT for 1 h and incubated overnight at 4 °C with primary antibodies and subsequently incubated with secondary antibodies conjugated with Alexa Fluor 488 ...
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bioRxiv - Genomics 2024Quote: ... Samples were then filled with BSA blocking buffer to a total volume of 500 µl and 0.75 µl of 7AAD (1 mg/ml, Sigma) was added.
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bioRxiv - Neuroscience 2024Quote: ... The blocking solution was then replaced with another consisting of 2 µL mouse anti-NeuN antibody (Merck Millipore, MAB377) and 0.5 µL of rabbit anti-GFAP antibody (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... in PBS for 20 minutes followed by 1 hour of blocking in 5% bovine serum albumin (BSA, Sigma-Aldrich). Primary antibodies were diluted in 5% BSA and incubated at 4°C overnight at given concentrations ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were permeabilised with 0.1% triton/PBS for 10 min and then blocked in blocking buffer (10% donkey serum/PBS (D9663, Sigma)) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed thrice with TBS-T buffer prior to addition of horseradish peroxidase (HRP)-conjugated secondary antibodies diluted in blocking solution (anti-mouse-HRP 1:10,000 or anti-rabbit-HRP 1:10,000 antibodies; all from Sigma Aldrich) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... was aspirated and the beads were then washed three times with TBS buffer supplemented with 0,1% digitonin and eluted by competition with 150μg/ml 3xFLAG peptide (Sigma) in TBS + 0,1% digitonin for 30 minutes at 4°C in an end over end shaker.
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were eluted with 5 packed bead volumes of 150 ng/μL 3xFLAG peptide (Sigma-Aldrich, F4799) in TBS for 30 min at 4 °C with rotation.
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bioRxiv - Immunology 2021Quote: ... The detached peptides were separated from HLA molecules using 3 kDa cut-off centrifugal ultrafilters (Millipore, MO, USA), and then concentrated using vacuum centrifugation.
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bioRxiv - Molecular Biology 2019Quote: ... beads were incubated with 0.2 bed volumes of Elution Buffer containing 5 mg/mL FLAG peptide (Sigma-Aldrich) for 10 min at 4°C and subsequently 0.6 bed volumes of Elution Buffer with PI were added and incubated with end-over-end rotation for 2 h at 4°C ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: ... 0.1% NP-40) and eluted in 43 µl of a 200 mg/ml dilution of FLAG peptides (Sigma) in 1x TBS ...
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bioRxiv - Immunology 2019Quote: ... Then,15 μg of peptides were purified using ZipTipC18 pipette tips according to manufacturer’s instructions (Millipore, Billerica, USA), resuspended in 2% Acetonitrile/0.1% formic acid solution ...
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bioRxiv - Biophysics 2019Quote: ... Peptide mixtures were separated using a gradient from 92% A (0.1% formic acid, FA, Sigma, in deionized water) and 8% B (0.1% FA in acetonitrile ...
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bioRxiv - Cell Biology 2020Quote: ... the proteins were eluted by adding 125 ng/ml FLAG peptide to the OG-containing buffer (Sigma-Aldrich) and incubating the beads for 30 min per round of elution ...
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bioRxiv - Cell Biology 2019Quote: ... Immune complexes were washed two times with lysis buffer and eluted with 0.5 mg/mL Flag peptide (Sigma) in lysis buffer ...
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bioRxiv - Bioengineering 2019Quote: ... The PEG-nanoparticle-peptide mixture was added to 15 mL of mineral oil (Sigma Aldrich, St. Louis, USA) with 1% v/v surfactant (Span 80/Tween 80 ...
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bioRxiv - Microbiology 2021Quote: ... For elution of FLAG-tagged proteins and their complexes 100 µL of 0.2 mg/mL FLAG3 peptide (Sigma) was added to samples ...
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bioRxiv - Biophysics 2020Quote: ... Elution was performed for 20 min at room temperature with TBS buffer supplemented with 3xFLAG peptide (Sigma-Aldrich) at a final concentration of 0.15 μg/ml.
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bioRxiv - Plant Biology 2021Quote: ... Protein complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... and then incubated with 3 resin volumes of 200 µg·ml-1 3xFLAG peptide (Sigma, catalog # F4799, lot # SLCJ4916) for 45 min to elute proteasome complexes ...
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bioRxiv - Microbiology 2021Quote: ... The beads were washed with 1x Tris-buffered saline (TBS) three times and proteins were eluted with 55μL of 100μg/mL 3xFLAG peptide (Sigma) in 1x TBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were eluted twice with equal bead volume (200 μl) of AP buffer supplemented with 3XFLAG peptide (Sigma) at 200 μg/ml at room temperature for 15 minutes on a Nutator.
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bioRxiv - Cell Biology 2021Quote: ... The resulting peptides were dried and reconstituted in 0.1% formic acid and desalted by C18 ziptip (EMD Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: The coding sequence of CHLI without transit peptide (1-46 amino acid residues) was cloned into pET32a (Novagen) and pGEX-4T-1 (GE healthcare ...
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bioRxiv - Biophysics 2022Quote: ... IP3R1 was eluted with 500 μM of T433 peptide and concentrated using a 100 kDa Amicon centricon (Millipore).
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bioRxiv - Microbiology 2020Quote: ... Peptides were dissolved in 2% acetonitrile containing 0.1% trifluoroacetic acid and desalted using C18 ZipTips (Merck Millipore, Germany). Each sample was independently analysed on a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... and again with wash buffer before elution buffer (wash buffer with 300 ng 3x-FLAG peptide (Sigma Aldrich)) was added and incubated with beads overnight ...
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bioRxiv - Bioengineering 2022Quote: ... Bis-cysteine crosslinking peptide VPM (GCNSVPMSMRGGSNCG) or non-degradable crosslinking agent DTT (1,4-dithiothreitol; Sigma, 3483-12-3) was dissolved in diH2O at 27.3 mM ...
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bioRxiv - Biochemistry 2019Quote: ... The pure recombinant protein was eluted from the column using the same buffer with 0.1 mg.mL-1 FLAG peptide (Sigma). Pure PRMT7 was flash frozen and stored at −80 °C ...
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bioRxiv - Systems Biology 2021Quote: ... stringent wash were done with modified lysis buffer (250 mM NaCl + 20 µg/ml peptide FLAG (F3290 Sigma)) prior to elution with 200µg/ml peptide FLAG ...
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bioRxiv - Molecular Biology 2020Quote: ... Bound protein was eluted three times for 30 min by incubation with 0.3 mg/ml 3xFLAG peptide (Sigma) in BC100 at 4°C with end-over-end rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was eluted using Buffer A with 150 mM NaCl and 200 μg/ml 3XFLAG peptide (Sigma). Peak fractions were pooled ...
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bioRxiv - Microbiology 2020Quote: ... Myc-tagged protein was eluted at room temperature with 25 μl myc-peptide (0.5mg/mL in PBS, Sigma) for 15 minutes at 700 rpm mixing ...
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bioRxiv - Cell Biology 2021Quote: ... TiO2 beads (5010-21315, GL Sciences) were used to enrich phosphorylated peptide and homemade C8 (66882-U, Sigma) stageTip was used to trap the TiO2 beads for washes ...
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bioRxiv - Neuroscience 2021Quote: ... 50 μg of each peptide was injected into the abdominal cavity of each mouse along with 0.25 mL Complete Freund’s Adjuvant (Sigma). To enhance the immune response ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM TCEP) before elution using wash buffer supplemented with 0.25 mg/mL 3xFLAG peptide (F4799, Sigma-Aldrich). The eluent was then subjected to another round of affinity purification using anti-HA resin (26181 ...