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Citations for Millipore Sigma :
2451 - 2500 of 5696 citations for Recombinant Human Insulin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Lysate containing FLAG-tagged target protein was purified using ANTI-FLAG M2 Affinity Gel resin (Sigma-Aldrich, USA) and eluted with 3x FLAG peptide (APExBIO ...
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bioRxiv - Cell Biology 2023Quote: 75 μg GST-tagged Rab GTPase was loaded with either 10 mM GDP or GTP (Sigma Aldrich, Germany) in the presence of 20 mM EDTA and 50 mM HEPES-NaOH ...
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bioRxiv - Molecular Biology 2023Quote: ... and subjected to binding of FLAG-tagged peptide species with anti-FLAG M2 magnetic beads (Sigma, cat # M8823). The purified FLAG-tagged peptides were harvested by eluting from the anti-FLAG M2 magnetic beads with 100 ng/µl 3xFLAG peptides.
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bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitation of Flag-tagged proteins was performed with Anti-Flag M2 Affinity Gel (Sigma-Aldrich, cat.no. A2220-1ML) at 4 °C with overhead rotation for 6 h ...
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bioRxiv - Biochemistry 2023Quote: ... GST-tagged protein was eluted off the beads by using 20 mM glutathione solution (Sigma-Aldrich, #G4251-5G). Fractions were analyzed by resolving on 4-20 % gradient SDS-PAGE (BioRAD #4561094 or #4651093) ...
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bioRxiv - Neuroscience 2021Quote: ... all mice were provoked with a convulsive dose (60 mg/kg; i.p.) of the GABAa receptor antagonist pentylenetetrazole (PTZ; Sigma-Aldrich, Co.) to measure seizure susceptibility and evaluate seizure thresholds (Dhamne et al. ...
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bioRxiv - Cell Biology 2022Quote: ... as well as the role of myosin II/RhoA in creating the refractory nature of the retracting losing front to receptor inputs (Fig. 5 and Supplementary Fig. 2) we pre-treated cells with either 50 μM nocodazole (Sigma Aldrich) or 10 μM Υ-27632 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Responsiveness to NGF was assayed by probing for the expression of tropomyosin receptor kinase A (Trk-A; 1:2000; #06-574, Millipore-Sigma) [48] ...
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bioRxiv - Neuroscience 2020Quote: ... Dimethyl sulfoxide (DMSO) and clozapine-N-oxide (CNO; agonist at hM3Dq receptors) were purchased from Sigma-Aldrich (St. Louis, MO, USA). A stock solution of CNO (0.1 mg/ml in saline solution containing 0.5% DMSO was prepared and stored in aliquots ...
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bioRxiv - Cell Biology 2021Quote: To explore the role of myosin II/RhoA and ERM proteins/cortical actin in creating a cell back refractory to receptor inputs we pre-treated cells with either 20 µM Υ-26732 (Sigma), or 50 µM Nocodazole (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... mice received vehicle or a cocktail of the a1-adrenergic receptor antagonist prazosin (0.5 mg/kg; Sigma-Adrich, St. Louis, MO) and the β-adrenergic receptor antagonist DL-propranolol (5 mg/kg ...
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bioRxiv - Neuroscience 2021Quote: ... with 10 pA increments in the presence of oxygenated R-aCSF supplemented with 10 μM gabazine and 1 μM strychnine (GABAA and glycine receptor antagonists, respectively; Sigma-Aldrich).
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bioRxiv - Neuroscience 2022Quote: ... For the blockade of cholinergic receptors in CA1 200nl of atropine (2 mM) and mecamylamine (2 mM) (1:1) (Sigma Aldrich) were microinjected at 1.2mm DV (IM-9B microinjector ...
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bioRxiv - Neuroscience 2022Quote: ... or the same volume of vehicle containing ghrelin receptor antagonist (GRA; [D-Lys3]-GHRP-6; 3.3mg/kg BW; Sigma-Aldrich G4535). This GRA dose was based on our previous report demonstrating its ability to restore cholecystokinin-induced NTS neuronal activation in food deprived rats [20] ...
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bioRxiv - Neuroscience 2022Quote: ... We blocked the nicotinic acetylcholine signaling pathway via application of the broad nicotinic receptor antagonists hexamethonium (Hex, 100 µM, Sigma Aldrich) and epibatidine (EPB ...
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bioRxiv - Neuroscience 2022Quote: Larvae were immobilized by immersion in a paralytic that specifically blocks nicotinic acetylcholine receptors present at the neuromuscular junction (20μL of 1 mg/mL α-bungarotoxin [Sigma Aldrich] in HEPES-buffered E3 [5 mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... sections were re-blocked in blocking buffer for 1 hour at room temperature then incubated in primary antibody solution containing mouse anti-5-HT1A receptor (Millipore Sigma, cat ...
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bioRxiv - Neuroscience 2023Quote: ... cells were treated for 1-2 hours prior to and for the duration of flow experiments with 1 µM S1P receptor antagonist fingolimod (FTY720) (stock 1 mM in DMSO, Sigma #SML0700). FTY720 experiments were performed with Protocol 1 in flow media supplemented with 0.3% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... mice and rats (male Sprague-Dawley, aged 7-10 weeks) were subcutaneously injected with the VEGF-receptor inhibitor SU5416 (Sigma-Aldrich) at a dosage of 20 mg/kg body weight under isoflurane anesthesia ...
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bioRxiv - Neuroscience 2024Quote: ... A rat anti-D1DR monoclonal antibody was used for D1DR-receptor staining (1:500; Sigma-Aldrich, St Louis, MO; Catalog #D2944). To enhance the GFP signals ...
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bioRxiv - Cancer Biology 2024Quote: ... In experiments testing the ability of small molecules to block serum-induced migration/invasion, 10 μM sc144 (a small molecule inhibitor of IL6ST, the signaling receptor for IL6) (Sigma, SML0763) and/or 100 nM DF2156A (ladarixin ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5 µM (NMDA receptor antagonist that preferentially binds to GluN2C/GluN2D (40)) or DL-2-Amino-5-phosphonovaleric acid (APV, Sigma, A5282) 100 µM (broad spectrum NMDA receptor blocker).
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bioRxiv - Cell Biology 2020Quote: The following antibodies were used procollagen VII (rabbit anti–human [Abcam]; mouse anti-human [Sigma-Aldrich]), Sec31A (mouse anti–human ...
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bioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation, Billerica, MA, USA), Fluo-4 AM and Pluronic F-127 (Molecular Probes ...
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bioRxiv - Pathology 2024Quote: ... RNA from human liver tissue and human cirrhotic PCLS was isolated using Tri Reagent (T9424, Sigma) and 1-bromo-3-chloropropane (B9673 ...
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bioRxiv - Neuroscience 2020Quote: ... human epidermal growth factor (hEGF; Sigma), recombinant human basic fibroblast growth factor (bFGF ...
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bioRxiv - Immunology 2021Quote: ... Commercially available human serum (Sigma-Aldrich) was used as the positive control.
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bioRxiv - Biochemistry 2020Quote: ... human liver cathepsin B (Millipore Sigma), and human cathepsin K (Enzo Sciences ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-human nuclei (Millipore MAB1281), mouse anti-TUJ1 (Covance MMS 435P) ...
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bioRxiv - Developmental Biology 2020Quote: ... of human chorionic gonadotrophin (hCG) (Sigma) 46 hours later ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with human albumin (Sigma, #A0237), sodium DL-lactate (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... human serum albumin (Sigma, Catalog # A9511) and human pituitary FSH (National Hormone and Pituitary Program ...
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bioRxiv - Immunology 2021Quote: ... purified human C1q (10μg/ml; Millipore) was added as a source of complement for 30 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 0.5% human serum albumin (Sigma Aldrich). Cell number in the suspension was adjusted to achieve a λ (mean number of cells per droplet ...
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bioRxiv - Cell Biology 2020Quote: ... TANGO1 (rabbit anti–human; Sigma-Aldrich), sec16A (rabbit anti-human ...
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bioRxiv - Bioengineering 2019Quote: ... human fibronectin (Merck-Millipore, Watford, UK) was premixed with Laponite gels to a final concentration of 50 µg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... sec16A (rabbit anti-human; Sigma-Aldrich), calreticulin (goat anti–human ...
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bioRxiv - Immunology 2019Quote: ... plus 10% human serum (Sigma,UK). IL-2 (100 IU/ml ...
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bioRxiv - Microbiology 2019Quote: ... Binding of human FH (Merck Millipore) was detected with Alexa-Fluor-488 labeled human FH ...
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bioRxiv - Biochemistry 2020Quote: ... 40 μM human serum albumin (Sigma) was included in the reaction ...
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bioRxiv - Neuroscience 2020Quote: ... albumin (human serum, Millipore Sigma #A9511), hemoglobin (human ...
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bioRxiv - Systems Biology 2020Quote: Human holo-transferrin (Sigma, cat: T4132) was coupled with thiorhodamine SOG at a molar ligand:SOG ratio of 1:1 and purified using 7-kDa ZebaSpin Columns (Thermo ...
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bioRxiv - Immunology 2021Quote: ... and human (AB serum, Sigma-Aldrich) in NuncTM 96 Deep-Well polystyrene plates (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... To aggregate human IgG (I4506; Sigma), its solution (2 mg/mL in PBS ...
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bioRxiv - Microbiology 2020Quote: ... Lyophilized human complement serum (Sigma, USA) was reconstituted in sterile phosphate-buffered saline (PBS) ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg/mL human transferrin (Sigma) and 30 nM sodium selenite (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... Human ARID1A antibody (Millipore: (04-080) Anti-BAF250a/ARID1a Antibody ...
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bioRxiv - Immunology 2022Quote: ... and 50% human AB serum (Sigma). Opsonized pathogen was co-incubated with phagocytes at a 1:1 or 5:1 CN:phagocyte ratio (c.p.r. ...
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bioRxiv - Microbiology 2022Quote: ... Human serum was purchased from Sigma.
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bioRxiv - Systems Biology 2022Quote: ... and human basic FGF (F0291, Sigma). Chemical perturbations were done with SU-5402 (SML0443 ...