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Citations for Millipore Sigma :
2351 - 2400 of 5696 citations for Recombinant Human Insulin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Human serum (Sigma-Aldrich, H4522) was thawed on ice and centrifuged at 18,000 ×g for 10 min at 4 °C to remove lipids ...
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bioRxiv - Immunology 2024Quote: ... 10% human serum (Sigma-Aldrich), 100 U/ml Penicillin (Cellgro Technologies LLC) ...
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bioRxiv - Bioengineering 2024Quote: ... Human thrombin (Sigma, #T6884-100UN) was reconstituted in sterile 0.9% saline supplemented with human serum albumin (0.1% w/v ...
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bioRxiv - Cancer Biology 2024Quote: ... thrombin (from human plasma, Sigma) and aprotinin (from bovine lung ...
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bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Sigma), 500 IU/mL of IL-2 (Proleukin S ...
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bioRxiv - Neuroscience 2020Quote: ... Dopamine signalling was manipulated by injecting the antagonist of D1 receptors SCH 23390 at a final estimated concentration of 1 μM (Sigma), the selective D2 receptor antagonist Sulpiride (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... For some experiments the AMPA/kainate glutamate receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 25 μM, Sigma-Aldrich), the NMDA glutamate receptor antagonist [3H]3-(2-carboxypiperazin-4-yl ...
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bioRxiv - Neuroscience 2021Quote: ... the NMDA glutamate receptor antagonist [3H]3-(2-carboxypiperazin-4-yl) propyl-1-phosphonic acid (CPP, 10 μM, Sigma-Aldrich) and a selective group III metabotropic glutamate receptor agonist ...
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bioRxiv - Neuroscience 2020Quote: ... Spontaneous IPSCs were isolated by blocking AMPA receptor-mediated events with CNQX (6-cyano-7-nitroquinoxaline-2,3-dione; 20uM; Sigma-Aldrich). Miniature events were isolated by blocking sodium channels with the addition of tetrodotoxin (1uM ...
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bioRxiv - Neuroscience 2021Quote: ... were dissolved in magnesium-free HEPES-aCSF in the presence of non-NMDA glutamate receptor blockers (CNQX, Tocris, 0190, 40 μM; Nimodipine, Tocris, 0600, 1 μM; LY-367385, Sigma, L4420 ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... The role of mannose receptor was evaluated by incubating cells with HIV-1 in the presence of 100µg/mL BMA (Sigma-Aldrich) or 100µg/mL bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2022Quote: Dopamine signalling was manipulated by injecting the antagonist of D1 receptors SCH 23390 at a final estimated concentration of 20 nM (Sigma). Finally ...
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bioRxiv - Neuroscience 2022Quote: ... Dbh−/− mice were treated with either saline or the non-selective DA receptor antagonist flupenthixol (0.25 mg/kg) (Sigma-Aldrich) to determine if excessive DA signaling contributes to grooming in these mice.
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bioRxiv - Neuroscience 2020Quote: ... Responsiveness to NGF was assayed by probing for the expression of tropomyosin receptor kinase A (Trk-A; 1:2000; #06-574, Millipore-Sigma) [48] ...
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bioRxiv - Neuroscience 2019Quote: ... Sheared crosslinked chromatin was immunoprecipitated separately with an anti-AR antibody (3 μg, 17-10489 ChIPAb + androgen receptor Assay Kit, Millipore) or an equal amount of normal IgG (3 μg ...
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bioRxiv - Neuroscience 2019Quote: ... We then removed the supernatant and incubated the lysates on ice for 1 hour with rabbit antibody to D2 receptors (1 μg per 100 μL, Millipore). Anti-rabbit Ig IP beads were added and samples were incubated overnight at 4°C with gentle agitation ...
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bioRxiv - Neuroscience 2021Quote: The following odors/concentrations were used for comparing molecular properties to receptor responses: 1% 2-methyl-2-pentenal (Sigma 294667), 1% trans-cinnamaldehyde (Sigma C80687) ...
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bioRxiv - Neuroscience 2021Quote: ... received bilateral infusions of either vehicle (aCSF; pH 7.4; males n = 5; females n = 4) or the GABAA receptor agonist muscimol (Sigma Aldrich, M1523 ...
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bioRxiv - Neuroscience 2022Quote: ... Currents of 5-HT3A receptors and engineered constructs expressed at the cell surface were evoked by applying 10 μM serotonin creatinine sulfate complex (Sigma), or in short 5-HT ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and incubated with mouse primary anti-Collagen Type II (DSHB II-II6B3) or mouse anti-dopamine 4 receptor (MABN125, Millipore) primary for 2 h at room temperature in a humidified chamber ...
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bioRxiv - Immunology 2024Quote: ... 8-Cyclopentyl-1,3-dipropylxanthine (DPCPX)(28, 33, 34) or A1 receptor agonist 2-Chloro-N6-cyclopentyladenosine (35) were purchased from Sigma Aldrich, dissolved in DMSO and filter sterilized by passing through a 0.22μm filter prior to use ...
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bioRxiv - Neuroscience 2022Quote: ... The expression of the receptor was quantified at both molecular weight forms using anti-DRD1 primary (Sigma Aldrich, cat#D2944) at 1:1000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... were dissolved in HEPES-aCSF in the presence of non-NMDA glutamate receptor blockers (CNQX, Tocris, 0190, 40 µM; Nimodipine, Tocris, 0600, 1 µM; LY-367385, Sigma, L4420 ...
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bioRxiv - Molecular Biology 2023Quote: ... and was mixed with 1 µg total DNA (100 ng receptor plasmid + 200 ng mGs plasmid + 700 ng salmon sperm DNA (Sigma) solubilized in 100 µL OptiMEM ...
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bioRxiv - Neuroscience 2023Quote: ... of 33% DMSO in artificial cerebrospinal fluid (Veh) or the serotonin type 2a receptor antagonist M100907 (1μg/side; M3324, Sigma-Aldrich).
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bioRxiv - Physiology 2023Quote: NIH-3T3-L1 cells were incubated in DMEM containing 2% fatty-acid free BSA in the presence of exogenous mitochondria and/or CL316,243 (β3-adrenergic receptor agonist; Sigma Aldrich) for 72 h ...
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bioRxiv - Neuroscience 2023Quote: ... and the AMPA receptor inhibitor 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 µM, Sigma Aldrich, St Louis, MO, USA). This solution was applied to the autaptic culture neurons for 2 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... anti-total-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptor (anti-tAMPAR) (Cat.#AB1504, Merck Millipore, Burlington, MA, USA), anti-phospho (Ser845)-AMPAR (anti-pAMPAR;cat.#AB5849 ...
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bioRxiv - Pathology 2020Quote: ... high-purity (>99%) human CRP obtained from human plasma (C4063; Sigma-Aldrich, Saint Louis, Missouri) was infused via the femoral vein after 45 minutes of ischemia with LAD ligation ...
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bioRxiv - Biophysics 2020Quote: ... Human Serum from human male AB plasma and NaOH 1N Bioreagent were purchased from Sigma. ss M13mp18 ...
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bioRxiv - Immunology 2023Quote: ... Human platelets were stimulated with 1 U ml−1 Thrombin from human plasma (Sigma-Aldrich) or left unstimulated for 30 min to assess the purity and activation of isolated cells ...
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bioRxiv - Biochemistry 2024Quote: ... and human apo-transferrin (P02787) isolated from human plasma were purchased from Sigma Aldrich (E0518), Invitrogen (RP-43132) ...
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bioRxiv - Genetics 2021Quote: ... ML-DmBG3-c2 cells were cultured in M3 + BPYE + 10% FBS with 10 µg/ml insulin (Sigma-Aldrich) while CME W1 Cl.8+ cells required M3 + 2% FBS + 5 µg/ml insulin + 2.5% fly extract containing medium ...
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bioRxiv - Cancer Biology 2022Quote: ... T47D in ATCC-formulated RPMI-1640 Medium (#30-2001) with 0.2 units/ml bovine insulin (Sigma-Aldrich, #I0516); SUM159 in RPMI with 2 mM glutamine ...
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bioRxiv - Cell Biology 2022Quote: ... Streptozotocin (STZ) to deplete insulin required administration of low doses of STZ (40 mg/kg; Sigma-Aldrich, S0130) injected intraperitoneally once-a-day for 5 consecutive days ...
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bioRxiv - Cell Biology 2022Quote: ... 14 h-fasted mice were subjected to an intraperitoneal injection of insulin (4.5 mg/kg; Sigma-Aldrich, I1882) and killed 30 min later as described13 ...
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bioRxiv - Physiology 2022Quote: ... The next day cells were incubated for 1 h with 30 nM insulin (Millipore-Sigma, Saint Louis, MO) prior to whole-cell patch-clamp recording.
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bioRxiv - Physiology 2022Quote: ... The next day cells were incubated for 1 h with 30 nM insulin (Millipore-Sigma, Saint Louis, MO) prior to whole-cell patch-clamp recording.
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bioRxiv - Neuroscience 2022Quote: ... culture medium I was replaced with culture medium II (DMEM supplemented with 24 μg/ml insulin (Sigma Aldrich), 100 μg/ml transferrin (EMD Millipore) ...
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bioRxiv - Physiology 2022Quote: ... Immunostaining of the pancreas was performed using a primary anti-insulin antibody (Catalog No-05-1066, Millipore, USA), followed by secondary anti-mouse Alexa 594 (1:800 dilution) ...
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Nuclei function as wedges to pry open spaces and promote collective, confined cell migration in vivobioRxiv - Cell Biology 2023Quote: ... with a pH of 6.85-6.95) and resuspended in live imaging media with 0.4 mg/mL bovine insulin (I1882, Sigma-Aldrich) and 1% agarose and immediately mounted for imaging ...
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bioRxiv - Cancer Biology 2023Quote: ... E-A14 and M-A14 cells and 10µg.mL−1 of Insulin-transferrin-sodium selenite media supplement (Sigma-Aldrich) for NEU15.
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bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... the blot was incubated with a mouse anti-His-tag primary antibody (Sigma-Aldrich, Steinheim, Germany), diluted 1:1 000 in TBS (10 mM TRIS/HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... The Trx-His(6) tag was cleaved by addition of 300 units of thrombin (Sigma T4648) directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes ...
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bioRxiv - Plant Biology 2020Quote: ... The His6-SEC5B fusion protein was extracted and purified with Ni-NTA His bind resin (Novagen). The freshly extracted resin-bound GST or GST-XIK proteins were mixed with purified His6-SEC5B protein in protein interaction buffer and incubated at 4°C for 2 h with shaking ...
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bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
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bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
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bioRxiv - Plant Biology 2021Quote: ... and GST were fused with a C-terminal octa-His tag in the pET42 vector (Novagen).