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Citations for Millipore Sigma :
2451 - 2500 of 10000+ citations for Medium Kit without Serum and with CultureBoost R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: Live embryos and larvae were first anesthetized in E3 media (without methylene blue) containing 0.16 mg/ml Tricaine (Sigma) and embedded for imaging in 2 % low-melting-point agarose/E3 (Sigma ...
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bioRxiv - Plant Biology 2019Quote: ... For Pchlide and heme assays seedlings were then grown for 4 d dark at 22 °C with or without 0.1 or 0.2 mM aminolevulinic acid (ALA; Sigma Aldrich) with 5mM MES ...
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bioRxiv - Immunology 2021Quote: ... Negative and positive controls included cells cultured without any stimulus and those cultured with PMA (250ng/ml) (Sigma-Aldrich) and Calcium Ionomycin (2.5μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: CM (without BSA) were concentrated using 3 kDa molecular weight cut-off (MWCO) Amicon ultracentrifugal units (Millipore, Burlington, MA) and lyophilized overnight ...
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bioRxiv - Bioengineering 2019Quote: ... or 48-well plates (with or without 7×7mm glass coverslips) pretreated with 50 µg/mL human fibronectin (Millipore).
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bioRxiv - Physiology 2020Quote: ... and the indicated LF or HF diet 435 g/kg were dissolved in heated water (1 kg) with or without BBR 1.45 g/kg (Sigma). The mixture was cooled at 4°C and cut into blocks which were freshly placed in animal hoppers every 2-3 days.
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bioRxiv - Systems Biology 2021Quote: Steroidogenic fetal adrenal cells were incubated in cultivation medium in each well with or without 100 nmol/l SST (hor-299-a, ProSprc-Tany) and 0.1mmol/l cholesterol (C3045, Sigma) intervention for 24 h(Damsteegt ...
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bioRxiv - Immunology 2020Quote: Purified spleen cDCs were plated in 96-well plate at 1×105 cells and incubated with or without 0.5 μg/mL LPS (Sigma) for 16 h before the quantification of IL-12p40 and IL-6 in the supernatant by ELISA (BD Biosciences) ...
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bioRxiv - Molecular Biology 2020Quote: ... or and 3) without 2i and LIF supplemented with 0.25 M all-trans-retinoic acid (Sigma-Aldrich, Cat #R2625), and cultured for 4 days.
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bioRxiv - Neuroscience 2022Quote: ... Animals were anesthetized with isoflurane and intracardially perfused with cold HBSS without cations with 5ug/ml actinomycin (Sigma A1410) and 10uM triptolide (Sigma T3652) ...
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bioRxiv - Bioengineering 2022Quote: ... Protein adsorption to PDMS surfaces without printed vitronectin was prevented by incubating coverslips in 0.2% Pluronic F127 solution (P2443-250G, Sigma) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... D-PBS was replaced with ice-cold blocking solution (3% BSA [Sigma, A3803] in D-PBS, without Ca2+/Mg2+) using a Dragonfly Discovery liquid dispenser (SPT Labtech ...
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bioRxiv - Cell Biology 2022Quote: ... Milk EV and serum EV samples required respectively 1:100 and 1:10-1:50 dilution in endotoxin-free Dulbecco’s 1X PBS without Ca++ and Mg++ (TMS-012-A Millipore) to reach the recommended concentration of 1*108- 1*109 particles/ml and number of particles in frame between 10 and 110 ...
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bioRxiv - Physiology 2022Quote: ... The same procedure was followed for Glycolysis Stress Test in non-buffered DMEM without glucose and glutamine (Sigma-Aldrich). Cells were monitored in basal conditions and in response to D-glucose (10 mM ...
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bioRxiv - Microbiology 2023Quote: ... or ½ LBS (half of LBS nutrients added) media without or with 5% (w/vol) of polyvinylpyrrolidone (PVP360, Sigma-Aldrich). To make LBS broth ...
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bioRxiv - Neuroscience 2022Quote: ... but without the use of iodoacetamide for cysteine alkylation due to the treatment of 50 mM N-ethylmaleimide (Sigma) during the sample immunoprecipitation ...
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bioRxiv - Neuroscience 2022Quote: ... Cell were sorted in single-cell drop mode into a tube containing DMEM without phenol red (D4947, Sigma-Aldrich), supplemented with GlutaMAX (35050061 ...
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bioRxiv - Microbiology 2024Quote: All strains were grown in LB at 37 °C until the culture reached an OD600 of 0.4 and then treated with or without 0.15 mM (final concentration) of MnCl2 (Sigma-Aldrich) for 60 min at 37 °C ...
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bioRxiv - Plant Biology 2024Quote: ... yeast was grown in selective agar media containing adenine {6.7 g/L yeast nitrogen base without aminoacids (Sigma Y0626), 0.64 g/L complete supplement mixture minus tryptophan (CMS-Trp) ...
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bioRxiv - Developmental Biology 2024Quote: 10 ug of GST-Nde1-T243A-T246A or GST-Nde1-S214F-T243A-T246A were incubated with or without 0.2 ug active Cdk5 or Cdk1 (Millipore), 400 uM ATP ...
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bioRxiv - Cancer Biology 2023Quote: ... or EIF4EBP1-targeting specific shRNAs (sh4E-BP1_1 or sh4E-BP1_2) were seeded in T25 flasks (TPP) and treated either with or without Dox (0.5 µg/ml; Sigma-Aldrich) for 96h ...
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bioRxiv - Biochemistry 2023Quote: Genes encoding VanX (C78/157S mutants with and without W24R) were constructed using the pET-47b plasmid vector (Novagen). The HRV3C recognition sequence and a linker to improve the digestion efficiency were inserted between the (His)6-tag and the vanx sequence ...
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bioRxiv - Cell Biology 2023Quote: ... the FSH-cultured follicles were then incubated with or without the PPP family phosphatase inhibitor cantharidin (C7632, Sigma-Aldrich) or the PKA inhibitor Rp-8-CPT- cAMPS (C011-05 ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 g/L NaCl) supplemented with 1.7 g/L yeast nitrogen base without NH4Cl and amino acids (Sigma Y1251). 1 g/L 15NH4Cl and 4 g/L glucose were supplemented for 15N labelling ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed and incubated at 37°C for up to 4 hours with or without 10µM bortezomib (Sigma) or 10nM BafA1 ...
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bioRxiv - Microbiology 2023Quote: ... Plates were incubated at 30 °C without shaking with lids coated with an aqueous solution of 20% ethanol and 0.01% Triton X-100 (Sigma) to prevent condensation19 ...
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bioRxiv - Cell Biology 2023Quote: ... The resin was then washed with 3x10 bed volumes of lysis buffer and eluted with 800 µL (for 60 ml of cell suspension) lysis buffer (without protease inhibitor) supplemented with 0.2 mg/mL 3x Flag peptides (Sigma). The N-terminal 3xFlag tag was removed by incubation with the GST tagged Prescission Protease (GenScript ...
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bioRxiv - Molecular Biology 2023Quote: ... For 1,6-hexanediol treatment cells were treated with 2 µg/ml digitonin with or without 10% 1,6- hexanediol (Sigma, #240117) in Shields and Sang M3 medium at 25°C for 1 h.
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bioRxiv - Microbiology 2023Quote: ... The supernatant was mixed with an equal volume of 0.2 M sodium citrate (pH 5.5) and incubated at 37°C for 8 h with or without (as a negative control) trehalase (Sigma). The amount of glucose generated from trehalose was assayed using a Glucose Assay Kit (Sigma ...
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bioRxiv - Biochemistry 2022Quote: Cells containing HT-PRC1 fusion gene were incubated without or with 0.4 µg/mL doxycycline hyclate (Sigma-Aldrich; D9891) for 72 hours prior to imaging ...
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bioRxiv - Pathology 2023Quote: ... HUVECs were then exposed to shear stress for 24 hours with or without Tubastatin-A (3 µM; Sigma-SML0044). For experiments where autophagic flux was blocked ...
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bioRxiv - Biochemistry 2022Quote: ... equal amounts of C2 and C0 TRAP1 were treated with or without 50 mM DTT (Sigma-Aldrich, Missouri, USA) to achieve total reduction ...
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bioRxiv - Cancer Biology 2023Quote: 2,000 MIA PaCa2 RELA-GFP cells/well were seeded in 384-well plates and treated the next day with the following drugs for 24 hr without TNFα at the specified dose ranges: Paclitaxel (6.25-200 nM; Sigma), Vinblastine (3.125-100 nM ...
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bioRxiv - Biophysics 2022Quote: ... pellets were resuspended with 90 μL of TE buffer and spread on an SDAA plate (yeast nitrogen base without amino acid 0.67 %, tryptophan 195 μM, agar 2 % (Sigma) and casamino acids 0.5 % (USBio)) ...
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bioRxiv - Biophysics 2023Quote: ... The EV sample was diluted in endotoxin-free Dulbecco’s 1X PBS without Ca2+ and Mg2+ (TMS -012-A Millipore). Three technical replicates were measured in various dilutions ranging from 1:500 to 1:1000 in a 60-second video under controlled temperature set at 20°C.
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was carefully collected without disturbing the loose pellet and sterile-filtered through a 0.22 µm PVDF filter bottle (Millipore), aliquoted and frozen at -20°C until used.
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bioRxiv - Microbiology 2023Quote: ... that blocks caveolar endocytosis without some of the off-target effects of MβCD (37) or the dynamin inhibitor dynasore (80 µM, Sigma) for 30 min prior.
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bioRxiv - Biophysics 2023Quote: Arabidopsis seeds were sown on half-strength MS media without or with the indicated concentration of ABA (Sigma-Aldrich), maintained in darkness at 4°C for 2 d ...
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bioRxiv - Immunology 2024Quote: ... NK-92/CD16A cells (3x105/ml) were treated with or without 10ng/ml PMA (Sigma Aldrich, St. Louis, MO) and 1μg/ml ionomycin (AdipoGen Life Sciences ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: After 4 hours cells were washed twice with 1 ml Neurobasal medium and changed subsequently to 2 mL of retinoic acid medium made of 1:1 minimal essential medium (MEM) (Sigma-Aldrich) and nutrient mixture F-12 Ham (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Directed differentiation was then initiated by changing StemFlex™ medium to neuralisation medium containing N2:B27 (N2 medium and B27 medium at a 1:1 ratio) supplemented with 100 nM LDN193189 (Sigma Aldrich) and 10 µM SB431542 (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... The growth medium was replaced by treatment medium [growth medium with corresponding concentration of PF-06672131 and/or PF-642899 (Sigma-Aldrich)] and incubated at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... the medium was replaced with medium containing 2X concentrated virus medium and 8 μg/ml polybrene (Millipore Sigma, TR-1003-G). The virus-containing medium was replaced with fresh medium 24 h post transduction.
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bioRxiv - Developmental Biology 2019Quote: ... the samples were incubated with the blocking buffer (0.1% Triton X-100, 5% normal donkey serum (NDS, Stratech) and 1% bovine serum albumin (BSA, Sigma)) for 30 minutes at RT ...
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bioRxiv - Physiology 2022Quote: ... we adapted the enhanced Perl’s Prussian Blue staining protocol3 by adding a blocking step with 5% Normal Goat Serum (16210064, Life technology) with 2.5 % Bovine Serum Albumin (10735078001, Sigma) for 60 min followed with 30 min of Peroxidase Blocking Solution (S2023 ...
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bioRxiv - Genomics 2024Quote: ... at a density of ∼2.5*106 cells/mL in 5% fetal calf serum (FCS) (Bodinco BDC, 16941) DMEM (Dulbecco’s Modified Eagle’s Serum (Sigma, 05796), 1% Pen-Strep (Lonza ...
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bioRxiv - Genomics 2024Quote: ... at a density of ∼2.5*106 cells/mL in 5% fetal calf serum (FCS) (Bodinco BDC, 16941) DMEM (Dulbecco’s Modified Eagle’s Serum (Sigma, 05796), 1% Pen-Strep (Lonza ...
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bioRxiv - Immunology 2023Quote: ... for 15 min at room temperature and blocked with 5% v/v goat serum in permeabilisation buffer (0.5% w/v bovine serum albumin and 0.1% w/v Saponin in PBS; Sigma-Aldrich). Permeabilisation buffer was used to dilute all antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues were incubated with blocking solution (0.5% Triton X, 2.5% bovine serum albumin [Sigma-Aldrich], 2.5% donkey serum [Sigma-Aldrich]) for one day at 37 °C before being incubated with the primary antibody at 1:500 for 3 days at 37 °C ...
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bioRxiv - Physiology 2023Quote: ... Unspecific antibody binding was blocked with 6% donkey serum or 4% goat serum (Sigma-Aldrich, St Louis, MO, USA), and 2.5% bovine serum albumin (BSA ...