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Citations for Millipore Sigma :
2451 - 2500 of 3691 citations for CD40 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 100 μL of goat anti-human IgG (Fc specific)-Peroxidase antibody (1 : 5000 dilution, Sigma) was added and incubated for another 1 h at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... Fibrin hydrogels were prepared by combining fibrinogen from human plasma (30 mg/mL Sigma-Aldrich) with thrombin from human plasma (1000 U/mL ...
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bioRxiv - Cell Biology 2022Quote: ... SYBR Green I (10,000× dilution; Cambrex Bioscience) and 0.3 μM human-specific primers (Sigma-Aldrich), as shown in Supplementary Table S1 ...
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bioRxiv - Neuroscience 2022Quote: ... Both human and mouse retinal explants were flat mounted on polytetrafluoroethylene organotypic filters (Millipore-Sigma) with the photoreceptor side down ...
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bioRxiv - Neuroscience 2022Quote: ... The following primary antibodies were used: Rabbit anti-human Aquaporin 4 (1:100, Millipore, AB3594), Rat anti-human CD44 (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... Akata cells were treated with 10 µg/ml of goat anti-human IgG (Sigma I1886) for 0 ...
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bioRxiv - Neuroscience 2022Quote: ... Two hundred microliters of medium containing 2 μM human insulin (Merck, Sigma-Aldrich Cat# I9278) or medium only was added to the thoraxes for 10 min (the final concentration of insulin was 1 μM) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 5% Human AB serum and 10 mM neutralized N-acetyl L-Cysteine (Sigma-Aldrich #A9165) supplemented with 30 units/mL IL-2 (NCI BRB Preclinical Repository ...
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bioRxiv - Synthetic Biology 2024Quote: ... 5% Human AB serum and 10 mM neutralized N-acetyl L-Cysteine (Sigma-Aldrich #A9165) supplemented with 30 units/mL IL-2 (NCI BRB Pre-clinical Repository).
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bioRxiv - Microbiology 2024Quote: ... Non-specific binding sites on the bacterial cells were blocked using human IgG (Sigma, UK) for an hour and washed with flow buffer again before incubated overnight with the rabbit immune sera (1:1,000 dilution) ...
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bioRxiv - Immunology 2024Quote: ... or goat anti-human secondary HRP antibody (Sigma-Aldrich, #A0170, 1:10,000, for baboon plasma) in 1% BSA in TBS for 1 hour at room 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... shRNA constructs in a pLKO.1 vector targeting human CHMP5 were purchased from Sigma Aldrich along with 2 control non-targeting shRNAs ...
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bioRxiv - Evolutionary Biology 2024Quote: ... filtered and used to infect human myoblasts assisted by polybrene (Sigma-Aldrich, TR-1003-G). When the culture reached 80-90% confluency ...
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bioRxiv - Immunology 2024Quote: ... Antigen-reactive mAbs were detected with goat anti-human IgG (Fab)-horseradish peroxidase (Sigma-Aldrich) conjugate diluted in PBS at a ratio of 1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: ... AML cell lines and human primary cells were cultured with RPMI 1640 (R8758 Sigma-Aldrich) medium complemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... followed by the adding 50 µg/ml (1:100) of human plasma fibronectin (Sigma-Aldrich) for overnight incubation at 4°C.
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bioRxiv - Bioengineering 2024Quote: ... A 3 μL drop of 2.5 U/mL human thrombin (Sigma-Aldrich, cat. no. T6884) was added to each well of the platform ...
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bioRxiv - Bioengineering 2024Quote: ... followed by incubation with CF-647 conjugated anti-human IgG (Sigma Aldrich, cat. no. SAB4600181) diluted 1:500 in cell staining buffer for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Both human and mouse retinal explants were flat mounted on polytetrafluoroethylene organotypic filters (Millipore-Sigma) with the photoreceptor side down ...
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bioRxiv - Neuroscience 2023Quote: SH-Sy5y human and N2a (Neuro-2a) murine cell lines were obtained from Sigma (US) and ATCC (US) ...
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bioRxiv - Cancer Biology 2022Quote: ... and 25 ng/ml of recombinant human Epidermal Growth Factor (EGF) (Sigma, Sitnyakovo Blvd, Bulgaria). Cells were then filtered through a 100 μm filter to retain the non-digested fragments ...
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bioRxiv - Microbiology 2023Quote: ... Bound antibody was detected using 1:20,000 dilution of HRP-conjugated anti-human IgG (Sigma) and plates developed using TMB substrate (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... Secondary antibody [goat anti-human IgG alkaline phosphatase (1:10000 in blocking solution; Millipore-Sigma). A solution of p-nitrophenylphosphate (Millipore-Sigma ...
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bioRxiv - Immunology 2022Quote: ... Secondary antibody [goat anti-human IgG alkaline phosphatase (1:10000 in blocking solution; Millipore-Sigma). A solution of p-nitrophenylphosphate (Millipore-Sigma ...
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bioRxiv - Molecular Biology 2023Quote: Human astrocytes were cultured on 0.1 mg/ml poly-D-Lysine (PDL; P6407, Sigma-Aldrich)-coated cover slips in 24 wells for 24 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by an intraperitoneal injection of 30 units of human chorionic gonadotropin (C1063, Sigma-Aldrich) ∼ 46 h later ...
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bioRxiv - Microbiology 2023Quote: Adhesion of Leptospira strains (Manilae WT, Δmpl36, Δmpl36+, and Patoc) to human PLG (Sigma-Aldrich) was assessed by ELISA as previously described [23] ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated with polyclonal rabbit antibodies recognizing human PLG (Sigma- Aldrich, 1:500), followed by peroxidase-conjugated secondary antibodies (1:10,000) ...
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bioRxiv - Immunology 2023Quote: ... Bound IgA or IgG was detected using alkaline phosphatase-conjugated goat anti-human IgA (Sigma) or goat anti-human IgG (Southern Biotech ...
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bioRxiv - Neuroscience 2023Quote: Human Embryonic Kidney 293T (HEK293T) cells were cultured in high-glucose DMEM containing glutamine (Sigma) supplemented with 10% FBS and 2% Penicillin-Streptomycin ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were maintained in fresh human erythrocytes at 2% hematocrit in complete RPMI-1640 (Sigma) medium in addition to 0.5% AlbuMaxII (Gibco by Life Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... was from Thermo Fisher Scientific (Waltham, MA) and human tissue plasminogen activator (tPA) from Sigma Aldrich (St. Louis, MO). Rabbit monoclonal anti-PAI-1 [EPR17796] (ab187263 ...
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bioRxiv - Cancer Biology 2023Quote: ... in the presence or absence of 100 pg/ml recombinant human (rh)-IL21 (Sigma-Aldrich). The next day ...
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bioRxiv - Biophysics 2023Quote: ... The anti-human Fc capturing mAb (GG-7) was from Sigma-Aldrich (St. Louis, MO). LIBS-2 was purchased from EMD Millipore (Billerica ...
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bioRxiv - Cancer Biology 2023Quote: ... DMEM medium supplemented with 10% human serum (male AB, H6914-100ml Batch SLBT2873, Sigma-Aldrich), 1 % penicillin/streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: Lentiviral particles encoding Flag-tagged human PDHA1 or shRNAs targeting mouse Pdha1 (TRCN0000041914/TRCN0000041914 (Sigma)) were produced in 293T packaging cells by transient transfection using Jet-PEI reagent (Ozyme) ...
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bioRxiv - Immunology 2022Quote: ... human CD3+ T cells were stimulated with 10ng/ml PMA and 1μM Ionomycin (Sigma-Aldrich) or with 1 μg/mL pre-coated α-CD3 and 1 μg/mL soluble α-CD28 ...
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bioRxiv - Cell Biology 2023Quote: ... Human colorectal adenocarcinoma cells (Caco-2, ATCC) were maintained in medium consisting of DMEM (Sigma), 100 U/ml penicillin ...
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bioRxiv - Developmental Biology 2023Quote: ... treated twice every 12 h with human recombinant sDLL4 (1µg/ml, Cat#: SRP3026, Sigma Aldrich) for a total of 24h ...
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bioRxiv - Immunology 2023Quote: ... Human acute monocytic leukemia cell line (THP-1) was cultured in RPMI-1640 (Sigma-Aldrich) supplemented with 10% heat-inactivated fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2023Quote: ... Human brain tissue was incubated in Sudan Black (0.3% Sudan Black B (Sigma, Cat# 199664) in 70% ethanol ...
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bioRxiv - Bioengineering 2023Quote: ... 100 µL of anti-human IgG (Fc specific)-peroxidase antibody produced in goat (Sigma, A0170) was added to each sample in a 1:40,000 dilution of PBS and incubated for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA encoding human PF4 ORF was cloned into the pET-15b vector (Novagen, Madison, WI) and transformed into Origami B (DE3 ...
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bioRxiv - Immunology 2023Quote: ... diluted with a 10x volume of RPMI with 10% heat-inactivated human AB serum (Sigma), and centrifuged at 300 x g for 7 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Primers for human H19 were used as a positive control for CTCF binding (Merck Millipore).
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bioRxiv - Cell Biology 2023Quote: Human αSN cDNA was transformed into Escherichia coli Strain BL21 (DE3) (Novagen, Madison, Wis., USA) using the pNIC28-Bsa4 plasmid ...
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bioRxiv - Genomics 2023Quote: ... 3.33 ml 75 mg/ ml Human Recombinant Albumin (A0237, Sigma-Aldrich, St Louis, MO, USA), 2.5 mL 1 M lactate in 1 M HEPES (L(+)Lactic acid sodium (L7022 ...
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bioRxiv - Biochemistry 2023Quote: ... sub-cultured until mid-exponential phase then opsonized in human serum (Sigma-Aldrich, no. H4522) for 30 min ...
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bioRxiv - Immunology 2023Quote: Human macrophages were harvested from culture using PBS+5mM Ethylenediaminetetraacetic acid (EDTA; Sigma Aldrich #E7889) and cell lifters (Fisher Scientific #08-100-240) ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blotted with the following antibodies: anti-human PSEN1-CTF (MAB5643; Merck Millipore); anti-PEN2 (D6G8 ...