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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for Dengue Virus Serotype 3 DIII envelope protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The pre-cleared protein solution was incubated with 3 μg anti-FLAG antibody (Sigma Aldrich, #F1804) or IgG (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... and bound proteins were eluted with 25 µl 1 mg/ml 3 × FLAG peptide (Sigma, F4799) in IP buffer containing 1% NP-40 and 1 × protease inhibitors at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... 2 mM EDTA and 1 % [v/v] protein phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich)) were added at a concentration of 2 mL/g tissue powder ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were immunoprecipitated by the addition of 3 µl of rabbit anti-FGFR3 (Sigma-Aldrich, F0425)/500 µg protein with protein A-agarose (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... induction of AID-tagged protein depletion was performed with 3-indole-acetic acid (IAA) (Sigma-Aldrich) during the exponential growth phase (approx ...
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bioRxiv - Biochemistry 2020Quote: ... The purified protein 5-HT2C or HCA2 (3 μg) was immobilized on nickel agarose beads (Sigma) in the incubation buffer containing 50 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore; Cat. No. UFC900324) at 3500 rpm and 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins were diluted 1:3 with Urea-buffer (4.5 mM Urea (Sigma-Aldrich Cat#U1250), 10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were eluted in buffer 5 [buffer 3 plus 200 μM FLAG peptide (Sigma-Aldrich)] for 1.25 hours ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound proteins were eluted with 150 μg/mL 3×Flag (Sigma-Aldrich, Cat# F4799) at 4 °C for 1.5 h ...
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bioRxiv - Immunology 2023Quote: ... Expression of the proteins of interest was induced by 3 μg/ml doxycycline (dox, Sigma-Aldrich) added to the culture medium ...
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bioRxiv - Cell Biology 2023Quote: ... TEV-eluted supernatant was added to 100 µL of S-protein agarose (69704-3, EMD Millipore) to capture S-tagged protein ...
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bioRxiv - Biophysics 2024Quote: ... The dissolved protein was concentrated using a 3 kDa centrifuge filter (Millipore Sigma, St. Louis, MO). HtrA1 variants (PDZ ...
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bioRxiv - Biophysics 2024Quote: ... The dissolved protein was concentrated using a 3 kDa centrifuge filter (Millipore Sigma, St. Louis, MO) to 70 uM ...
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bioRxiv - Biochemistry 2024Quote: ... The protein mixture was concentrated using Amicon Ultra 3 kDa MW cut-off device (Merck Millipore) to ∼15 mg/ml and used to set up commercial crystallisation screens using the sitting drop vapour diffusion method at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... The diluted proteins were incubated with primary amine reactive 3 mM (final) bis(sulphosuccinimidyl)suberate (Sigma) and 20 µL of the sample was taken at various time points (0 ...
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bioRxiv - Genetics 2021Quote: ... Each ORF cloned into the pLVX-EF1a-IRES-PURO/eGFP lentiviral expression vector was added to lentiviral packaging and envelope vectors (Sigma-MISSION-gag-pol and the envelope Sigma-MISSION-VSV-G (Cat ...
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bioRxiv - Molecular Biology 2022Quote: Mammalian lentiviral particles harboring sgRNA-encoding plasmids or cDNA-encoding plasmids were co-transfected with the psPAX2 envelope and VSV-G packaging plasmids into actively growing HEK-293T cells (ATCC) using Xtremegene-HP (Sigma) transfection reagent as previously described (Sarbassov et al. ...
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bioRxiv - Cell Biology 2024Quote: HEK293T cells were transfected with retroviral plasmids along with packaging plasmids and VSV-G envelope using XtremeGene9 transfection reagent (Sigma). After 16 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus was then filtered with a 0.45 µm PVDF filter (SLHV013SL, Millipore) and mixed with polybrene (TR-1003-G ...
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bioRxiv - Genetics 2021Quote: ... Increasing amounts of virus and 8 μg/ml polybrene (hexadimethrine bromide, Sigma) were added to each well ...
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bioRxiv - Microbiology 2020Quote: ... The virus was propagated once in Vero E6 cells in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Systems Biology 2020Quote: ... Virus was concentrated using Amicon Ultra-15 Centfigiual Filter Concentrators (Millipore UFC910008). Virus was titered by plaque assay ...
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bioRxiv - Microbiology 2021Quote: ... influenza virus NP (clones A1 and A3, MAB8251, Sigma-Aldrich, 1:100) and anti-mouse IgG AlexaFluor-488 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... virus-containing supernatants were filtered through a 0.45 μm membrane (Merck Millipore). The amount of virus in the supernatants was quantified by RT assay ...
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bioRxiv - Immunology 2022Quote: ... virus particles were precipitated by adding 50% (w/v) PEG6000 (Sigma-Aldrich) in NT buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... Virus was backfilled into a pipette of mineral oil (M5904, Sigma-Aldrich). We injected 400nl virus into the identified C2 barrel column through a single injection site over 4 min at 300μm below pia ...
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bioRxiv - Immunology 2020Quote: Inactivated virus was concentrated using Amicon Ultra Centrifugal Filter units (Millipore Sigma) at 100k cutoff ...
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bioRxiv - Microbiology 2020Quote: ... Unabsorbed virus were removed and cells were overlaid with MEM (Sigma-Aldrich) containing 0.65% agarose (Nacalai tesque ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transduced with virus along with 10 μg/mL polybrene (Sigma). Following transduction ...
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bioRxiv - Genomics 2024Quote: ... virus-containing supernatant was filtered through a 0.45 μm filter (Merck Millipore), aliquoted ...
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bioRxiv - Neuroscience 2023Quote: ... The virus-infected GL261 cells were trypsinized with 0.25% trypsin (Sigma-Aldrich) 72 hours after infection ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was then filtered with a 0.45 µm PVDF filter (SLHV013SL, Millipore) and mixed with 4-fold volume of fresh media containing 12.5 µg/ml polybrene (TR-1003-G ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was then filtered with a 0.45 µm PVDF filter (SLHV013SL, Millipore) and mixed with 4-fold volume of fresh media containing 12.5 µg/ml polybrene (TR-1003-G ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus was concentrated using Amicon Ultra filters (Millipore cat. no. UFC910024) until they have about 1.5ml of concentrated virus ...
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bioRxiv - Cell Biology 2023Quote: ... where cells and virus were mixed with polybrene (Millipore, TR-1003-G) in antibiotic-free media at 2 M cells per well of a 12-well plate ...
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bioRxiv - Cell Biology 2023Quote: ... where cells and virus were mixed with polybrene (Millipore TR-1003-G) in antibiotic-free media at 2 M cells per well of a 12-well plate ...
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bioRxiv - Neuroscience 2024Quote: ... 100-300 nl virus containing 0.05% Fast Green FCF (Sigma, F7252-5G) was delivered to three sites (left CG ...
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bioRxiv - Biophysics 2024Quote: ... Virus was mixed with BSA-coated 10 nm colloidal gold (Sigma-Aldrich) and 3 µL were applied to glow-discharged R2/2 holey carbon copper grids (Quantifoil) ...
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bioRxiv - Cancer Biology 2021Quote: ... PreScission protease-eluted supernatant was added to 100 mL of S-protein agarose (EMD Millipore, 69704-3) to capture S-tagged protein ...
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bioRxiv - Microbiology 2021Quote: Poly-histidine tags were cleaved from proteins by treating them with 3 units of TEV protease (Sigma) overnight at 4 °C based on the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were concentrated using Amicon Ultra-15 centrifugal filters (3 kDa molecular weight cut-off, Millipore). Reducing agent (2 mM DTT ...
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bioRxiv - Biochemistry 2020Quote: ... Bound proteins were eluted from magnetic beads with 1 mg/ml of 3×FLAG peptide (Sigma F4799) with agitation at 4 °C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Protein degradation rate was measured after 3-hour treatment with 20μg/ml of cycloheximide (Sigma-Aldrich #C7698) in the complete RPMI medium ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were eluted by the addition of elution buffer containing 3 x Flag peptide (Sigma, cat. #F4799) at 4°C for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were extracted either in 100 μl 3 M guanidinium hy-drochloride (Gnd-HCl, Sigma-Aldrich, G3272) in 0.1 M Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 mg total protein were mixed with 2 mL Urea Buffer (UB) (8 M urea (Sigma, U5128) in 0.1 M Tris/HCl pH 8.0 and 50mM ammonium bicarbonate) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated to approximately 3 mg/ml using an ultrafiltration pressure cell (Millipore, Billerica, MA) with a 10-kDa molecular mass cutoff membrane and buffer exchanged with 20 mM HEPES ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein elution was carried out under native conditions by competition with commercially available 3× FLAG peptide (Sigma). Eluted proteins separated in 4-20% polyacrylamide gels in denatured conditions were visualized for purity after Coomassie blue (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Protein-containing fractions were pooled and concentrated on an Amicon 3 kDa MWCO spin concentrator (Merck Millipore). After another IMAC purification step using Ni-NTA resin ...