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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Dengue Virus Serotype 3 DIII envelope protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100µl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Microbiology 2021Quote: ... All virus propagation was performed in VeroE6 cells cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Systems Biology 2022Quote: ... virus particles were precipitated by adding 50% (wt/vol) polyethylene glycol 6000 (PEG 6000) (Sigma-Aldrich) in NT buffer (150 mM NaCl ...
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bioRxiv - Cancer Biology 2024Quote: ... and concentrated using fast-trap virus purification and concentration kit according to manufacturer′s instructions (Millipore). For transduction ...
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bioRxiv - Cell Biology 2023Quote: ... culture supernatants containing virus were harvested and passed through a 0.45 μM PVDF filter (Millipore, SLHV033RS). Viral titers were calculated and an MOI of 1 was used for transduction ...
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bioRxiv - Microbiology 2024Quote: ... the virus/glycan-mixture is incubated with 0.5 U heparinase I and III (H3917, Sigma-Aldrich) for 1 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... The virus containing supernatant was mixed with polyethylene glycol 6000 (Sigma Aldrich, Saint Louis, Missouri, US) (8 % wt/v ...
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bioRxiv - Microbiology 2024Quote: ... The concentrated supernatant containing the virus was layered on a discontinuous 20/55% OptiPrep (Sigma-Aldrich) density gradient and ultracentrifuged at 40,000 rpm for 2 hours at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The collected virus-containing medium was filtered through a 0.45 μm syringe filter unit (Millipore Sigma) and stored at −80°C for future use.
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bioRxiv - Bioengineering 2024Quote: ... Virus-containing supernatant was filtered using Steriflip™ Sterile Disposable Vacuum Filter Units (Millipore Sigma SEIM003M00). Virus was concentrated using Retro-X Concentrator (Takara ...
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bioRxiv - Cancer Biology 2024Quote: ... The collected virus-containing medium was filtered through a 0.45 μm syringe filter unit (Millipore Sigma) and stored at −80°C for future use.
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bioRxiv - Microbiology 2024Quote: ... We used poly((R)-3-hydroxybutyrate-co-(R)-3-hydroxyvalerate-co-(R)-3-hydroxyhexanoate) (Sigma-Aldrich, USA) to generate the standard curve.
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bioRxiv - Neuroscience 2020Quote: ... DAB (3-3’-diaminobenzidine, Sigma-Aldrich catalog no. D8001-10G) was dissolved in 0.1 N HCl at a concentration of 5.4 mg ml-1 and subsequently diluted ten-fold into sodium cacodylate buffer (pH 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... SD-Leu-Trp-His+1mM 3-AT (3-aminotriazol, Sigma) and SD-Leu-Trp-His-Ade (Formedium).
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bioRxiv - Developmental Biology 2022Quote: ... The reaction was developed using 3-3’-Diaminobenzidine (DAB) (Sigma). For CLASP1 immunostaining ...
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bioRxiv - Biochemistry 2021Quote: ... 2-amino-3-phosphonopropionic acid (AP-3, Sigma-Aldrich A4910), ATP (Sigma-Aldrich A2383) ...
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bioRxiv - Immunology 2020Quote: ... E-64 and 3-Methyladenine (3-MA) were from Sigma. Necrostatin-1 was from Abcam ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC; Sigma Aldrich #39391), and 3NPH solutions were mixed ...
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bioRxiv - Neuroscience 2023Quote: ... (3) the PDE4B inhibitor A33 (3 mg/kg, Sigma-Aldrich), (4 ...
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bioRxiv - Immunology 2024Quote: ... 3-nitropropionic acid (3-NP, several doses, Sigma Aldrich N5636), Atpenin-A5 (several doses ...
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bioRxiv - Molecular Biology 2024Quote: ... Glycogen Synthase Kinase 3 (GSK3) inhibitor CHIR99021 (3 μM) (Millipore) and Leukaemia-Inhibitory Factor (LIF ...
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bioRxiv - Plant Biology 2024Quote: ... in the presence of 3-aminotriazol (3-AT) (Sigma-Aldrich). Quantitative interaction assays were performed in liquid medium by quantifying β-galactosidase activity as previously described (Hernández-García et al. ...
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bioRxiv - Neuroscience 2020Quote: ... 3% BSA (Sigma), 0.3% Sodium azide (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... 3 (Sigma Aldrich). For the immunofluorescence staining of freshly extracted zfPGCs ...
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bioRxiv - Microbiology 2020Quote: ... 3% milk (Sigma). Primary antibody detecting ...
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bioRxiv - Cancer Biology 2022Quote: ... 3% BSA (Sigma) and 0.1% Tween-20 (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mixture (Sigma). Cell lysates were then centrifuged at 15000×g for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... Relaxin-3 (Sigma), prostaglandin E2 (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... 3% BSA (Sigma) in TBS ...
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bioRxiv - Cell Biology 2021Quote: ... 3% BSA (Sigma)) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... 3 (Sigma-Aldrich) and eosin Y (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2022Quote: ... 3% sucrose (Sigma) and 0.8% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... 3 (Sigma-Aldrich). Documentation was performed with an Axioskop 2 mot plus (Zeiss ...
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bioRxiv - Developmental Biology 2023Quote: ... 3% BSA (Sigma), 0.5% triton X100 (Sigma) ...
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bioRxiv - Pathology 2024Quote: ... 3% BSA (Sigma), 0.5% Triton X-100 (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... 3% BSA (Sigma), 1.2mM Calcium Chloride ...
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bioRxiv - Genetics 2021Quote: ... Proteins were purified by Nickel-NTA resin and treated with TEV protease (1 mg lab made TEV per 40 mg of protein) and benzonase (100 units/ml, Novagen 70664-3) overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: For RNA-binding protein immunoprecipitation (RIP) ~3×107 A549 cells for each sample were processed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: To induce the degradation of mAID-fused proteins, cells were treated with 500 µM indole-3-acetic acid (auxin, IAA, dissolved 500 mM in water) (Millipore Sigma, I5148) for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... rinsed with PBS and the proteins were visualized with Immobilon Western Chemiluminiscent HRP substrate (Merck/Millipore, used at 1:1:3 (H2O) dilution ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Plant Biology 2022Quote: Kinase activity assay was carried out by mixing 2 μg of CORK1KD or GST-CORK1KD-G748E with 3 μg of myelin basic protein (MBP; Sigma-Aldrich, Germany) in kinase reaction buffer (25 mM Tris-HCl ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... SDS samples containing 4∼8 μg total proteins were separated with 12% HEPES-Tris PAGE gel (MEILUNBIO, MA0243) and transferred to polyvinylidene difluoride membranes (Millipore, 70584-3). The membrane was blocked with 5% skim milk in TBST buffer (20mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... For NMR-experiments the proteins were concentrated by repeated ultrafiltration (Amicon Ultra-4 Ultracel-3 kDa centrifugal filter device, Merck Millipore, Burlington, USA).
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bioRxiv - Plant Biology 2024Quote: ... Purified proteins were visualized by Coomassie-stained SDS-PAGE and concentrated using a 3 kDa MWCO Amicon Ultra centrifugal filter (Millipore Sigma, #UFC9003,).
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bioRxiv - Genomics 2024Quote: ... Protein was purified by Nickel-NTA resin and treated with TEV protease (1mg lab made TEV per 40mg of protein) and benzonase-100units/ml (Novagen, 70664-3) overnight at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... to calculate the volume equivalent of 100 µg of protein before loading onto a 3 kDa molecular weight cutoff (MWCO) filter (Merck Millipore, Bayswater, Australia). Tryptic peptides were prepared using the FASP protocol as previously described (75) ...
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bioRxiv - Molecular Biology 2020Quote: ... Sph2-YM4271 cells were grown on synthetic dropout (SD)-His media supplemented with the inhibitor 3 mM 3-amino-1,2,4-triazole (3-AT; Sigma). All Y1H effector plasmids were separately transformed into the Sph2-YM4271 reporter strain and plated on SD/-Leu plates ...
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bioRxiv - Cell Biology 2020Quote: ... Virus was collected 24- and 48-hours post-transfection and filtered through a 0.45µm filter (EMD Millipore), and used for transduction of 4T07 ...
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bioRxiv - Molecular Biology 2021Quote: ... After 2h of virus adsorption in the presence of 0.2 mg/ml of porcine pancreatin (Sigma-Aldrich) prepared in CMGF(-) ...