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Citations for Millipore Sigma :
2401 - 2450 of 10000+ citations for Protein DBF4 Homolog A DBF4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... proteins were reduced in 10 mM dithiothreitol (Sigma Aldrich, UK) for 30 min at 37°C and alkylated in 55 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were transferred to a PVDF membrane (IPVH00010, Immobilon, Millipore) and blocked for 1 h with Tris-buffered saline containing 0.05% Tween 20 and 3% bovine serum albumin ...
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RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent mannerbioRxiv - Molecular Biology 2023Quote: ... Proteins were transferred to polyvinylidene difluoride membranes (IPFL00010, Millipore, USA) and incubated with specific antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted proteins were concentrated using Amicon spin concentrators (Merck Millipore) and subjected to gel filtration (Superdex 75 and 200 16/60 pg ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were purified from lysate using either nickel (Sigma-Aldrich) or glutathione resin (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... and whole-protein lysate was extracted using Bugbuster reagent (Novagen) and Pierce Universal Nuclease according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... Proteins from purchased powder-stocks were equine myoglobin (Sigma-Aldrich), bovine ribonuclease A (RNaseA ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were transferred to Immobilon-P membrane (Merck Millipore Ltd.) and westerns preformed with Ift140 (23 ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were transferred to a PVDF membrane (Immobilon, Millipore), which was subsequently blocked for 1 h at room temperature (5% Marvel milk (Premier foods ...
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bioRxiv - Physiology 2023Quote: ... and protein transfer was performed using a PVDF membrane (Millipore). Blots were blocked with LI-COR PBS-blocking buffer and probed with the following primary antibodies at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... All the eluted proteins were concentrated by centrifugal filtrations (Millipore), loaded onto desalting columns (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... Protein was precipitated using ice-cold acetone (Sigma, MS grade), 4 times the volume of the cell lysate and by incubating at -20°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were then transferred onto PVDF membrane (Millipore, IPVH00010) at 400 mAmp for 90 min on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein complexes were eluted with 3x-FLAG peptide (Sigma-Aldrich) or HA peptide (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were transferred into nitrocellulose membranes (Merck-Millipore, Germany) for 2h at 300 mV using an electrophoretic transfer system (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were then transferred to a PVDF membrane (Millipore) using a semi-dry transfer apparatus at 10V for 30 minutes at room temperature and imaged under the stain-free blot setting on Chemi-Doc to visualize the total transferred proteins ...
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bioRxiv - Microbiology 2023Quote: ... proteins were alkylated with 10 mM IAA (Sigma-Aldrich, Germany) in 100 mM TEAB at 25 °C for 20 min in the dark ...
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bioRxiv - Genomics 2023Quote: ... per 20 μl of protein A/G MagnaBeads (Merck Millipore). Beads were then washed once with low salt wash buffer (0.1% SDS ...
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bioRxiv - Immunology 2023Quote: ... Bound proteins were reduced with 5 mM DTT (Sigma-Aldrich) in 50 mM TEAB (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... The resolved proteins were transferred onto a PVDF membrane (Millipore) using a semi-dry blotting system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was expressed E.coli strain Rosetta 2 (DE3) (Novagen). The cells were grown at 37 °C in Luria-Bertani medium (LB ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein G Mag Sepharose beads (Sigma-Aldrich GE28-9513-79) were washed in cell lysis buffer and blocked in 10% BSA (Jackson ImmunoResearch 001-000-162 ...
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bioRxiv - Cell Biology 2023Quote: ... The eluates were incubated with S-protein agarose beads (Novagen) for 1 h at 4°C and then washed thrice with NETN buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and the protein amounts were determined by BCA assay (Sigma). The proteins were denatured by boiling for 10 min in the DTT-containing sample buffer and separated by 4–20% Mini-PROTEIN TGX gels (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Total protein was visualized using Ponceau S stain (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... The proteins were concentrated using Amicon Ultra Centrifugal Filters (Millipore).
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bioRxiv - Biophysics 2023Quote: ... followed by protein concentration via Amicon Ultra 10 MWCO (Millipore). EPR Spectroscopy of Spin-Labeled Grh1 ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were reduced with 4 mM DTT (Sigma-Aldrich) for 60 min at room temperature and alkylated in the dark using 8 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein content was previously checked with Ponceau (Sigma-Aldrich, #P7170) and membranes (Merck ...
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bioRxiv - Microbiology 2023Quote: ... followed by transfer of proteins to an Immobilon membrane (Millipore) using CAPS buffer (CAPS 10mM ...
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bioRxiv - Neuroscience 2023Quote: ... Total transferred protein was visualized with Ponceau S (Sigma-Aldrich) and incubated overnight in blocking buffer (Intercept Blocking Buffer ...
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bioRxiv - Cell Biology 2024Quote: ... concentrated to 500 µl using an appropriate protein concentrator (Millipore) and further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare ...
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bioRxiv - Plant Biology 2024Quote: ... GST-tagged protein fractions were incubated with thrombin (Sigma, Germany) at 4°C overnight for cleavage of the GST-tag (Step 2 ...
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bioRxiv - Biochemistry 2024Quote: ... and an Atto 647N protein labeling kit (Sigma-Aldrich, 76508) were used ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected using the Crescendo Western Blotting substrate (Millipore).
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected using the Crescendo Western Blotting substrate (Millipore). The other half of the beads were resuspended in the TRI reagent for subsequent RNA extraction and cDNA synthesis.
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bioRxiv - Cell Biology 2024Quote: ... and recombinant protein production was induced via IPTG induction (Sigma). Cells were lysed with BugBuster HT (Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... the protein was extracted with 1X RIPA buffer (Sigma-Aldrich) supplied with EDTA-free Protease Inhibitor Cocktail (cOmplete™ ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were precipitated by adding 100% trichloroacetic acid (Sigma 76039) to a final concentration of 20% v/v and incubated on ice for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto polyvinylidene fluoride (PDVF) membranes (Millipore, IEVH00005). Blocking was performed with 5% non-fat milk dissolved in TBST for 1 hour followed by incubation with primary antibodies PRKN (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2024Quote: ... The resolved proteins were then transferred onto nitrocellulose membranes (Millipore), followed by blocking with 5% BSA (Merck).
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bioRxiv - Cancer Biology 2024Quote: ... and proteins were transferred to PVDF membrane (Millipore, cat. ISEQ00010). The membranes were then blocked with 5% non-fat dry milk (Kroger ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was concentrated using an Amicon filter unit (Millipore) down to 1 mL ...
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bioRxiv - Immunology 2024Quote: ... 30 μl of Protein G Sepharose™ beads (#P3296, Millipore) were added and incubated for 2 hours at 4°C with rotation ...
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bioRxiv - Cancer Biology 2024Quote: ... and surfactant protein-C (SPC, EMD Millipore, #AB3786, AT2 marker). To investigate the role of transferrin ...
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bioRxiv - Microbiology 2024Quote: ... BugBuster Lysozyme (BBL) solution (99% BugBuster Protein Extraction reagent (Millipore) and 1% egg white lysozyme (GoldBio ...
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bioRxiv - Microbiology 2024Quote: ... 6g/L peptone (from casein and other animal proteins, Sigma), 2 mL/L glycerol (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... BugBuster Lysozyme (BBL) solution (99% BugBuster Protein Extraction reagent (Millipore) and 1% egg white lysozyme (GoldBio ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were concentrated using Amicon Ultra-15 centrifugal filters (Millipore; 10K MWCO for TamA ...
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bioRxiv - Immunology 2024Quote: ... Hydrophobic high protein binding membranes 96-well plates (Millipore Sigma) were hydrated with 40% ethanol ...