Labshake search
Citations for Millipore Sigma :
2201 - 2250 of 10000+ citations for Protein DBF4 Homolog A DBF4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... Proteins were reduced with 5 mM dithiothreitol (DTT) (Sigma-Aldrich) for 30 min at 55 °C and then alkylated by the addition of 10 mM iodoacetamide (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2022Quote: ... the bound proteins were eluted with 3X FLAG peptide (Sigma). The purity of DNA2 proteins was analyzed by 4–15% gradient SDS–polyacrylamide electrophoresis (SDS–PAGE ...
-
bioRxiv - Molecular Biology 2022Quote: Protein lysates were generated by incubation with RIPA Buffer (Sigma) supplemented with 1% complete protease inhibitor cocktail (Sigma ...
-
bioRxiv - Biophysics 2022Quote: ... FN3 proteins were expressed in Rosetta DE3 cells (EMD Millipore), lysed by sonication ...
-
bioRxiv - Cancer Biology 2022Quote: Protein was extracted using Radioimmunoprecipitation Assay (RIPA) buffer (Sigma- Aldrich) supplemented with 1 mM EDTA ...
-
bioRxiv - Neuroscience 2022Quote: ... astrocytic marker glial fibrillary acidic protein (GFAP) (chicken, Millipore, AB5541), microglial marker ionized calcium binding adaptor molecule 1 (Iba1 ...
-
bioRxiv - Plant Biology 2019Quote: ... before capture of immune complexes with Protein A agarose (Millipore). Crosslink reversal and protein removal was performed as described previously (Nelson et al. ...
-
bioRxiv - Plant Biology 2019Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Recombinant proteins were expressed in either BL21 or Rosetta (Novagen) cells ...
-
bioRxiv - Molecular Biology 2019Quote: ... and purified by protein A-agarose beads (16-157; Millipore). NIPBL ChIP-seq were from recently published data [46] ...
-
bioRxiv - Genetics 2019Quote: ... Proteins were transferred to Immobilon-P PVDF membrane (Millipore Sigma) at 400 mA for 40 min on a Trans-blot SD semi-dry transfer cell (Bio-Rad ...
-
bioRxiv - Neuroscience 2019Quote: ... 100 μM SAMS (substrate for AMP-activated Protein Kinase, Millipore), 0.2 μCi (γ-32P ...
-
bioRxiv - Synthetic Biology 2020Quote: ... proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore), blocked using 5% bovine serum albumin (BSA ...
-
bioRxiv - Biochemistry 2019Quote: ... the protein was eluted with 10 mM reduced GSH (Sigma) in 10 mM Tris-HCl ...
-
bioRxiv - Genomics 2019Quote: ... Proteins were transferred onto Immobilion-P PVDF membranes (Millipore Sigma) by means of tank blotting in 1X Tris/Glycine (Bio-Rad ...
-
bioRxiv - Physiology 2019Quote: ... G-Sepharose beads (50µL for 2mg of total proteins, Sigma) were added for 1h before being washed with lysis buffer 3 times ...
-
bioRxiv - Bioengineering 2019Quote: ... 20μg protein was incubated in 10% 2-Mercaptoethanol (Sigma-Aldrich) in laemmli loading buffer (Bio-Rad ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins were electroblotted onto Immobilon-P transfer membranes (Millipore Corporation). The membranes were blocked with Blocking One (Nacalai Tesque ...
-
bioRxiv - Immunology 2019Quote: ... Protein concentrations were read by a MAGPIX from EMD Millipore using xPONENT 4.2.
-
bioRxiv - Biochemistry 2020Quote: ... 100 µg protein were dot blotted on PVDF membrane (Millipore), stained with Alcian blue and excised ...
-
bioRxiv - Genetics 2019Quote: ... The TAP-tagged proteins were probed with PAP (Sigma-Aldrich) (1:2,000 ...
-
bioRxiv - Zoology 2020Quote: ... in comparison with wells containing visible protein ladder (Sigma 26616). The antibody staining intensity of each Western blot sample was normalised to its corresponding total-protein stain intensity using BioRad ImageLab software ...
-
bioRxiv - Biochemistry 2019Quote: ... Solubilized proteins were applied to FLAG-M2 agarose (Sigma-Aldrich) pre-equilibrated with buffer A ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein expression was induced with 200 pg/mL doxycyclin (Sigma). Cell cycle synchronization was performed using a combination of 2.5 mM thymidine (Sigma ...
-
bioRxiv - Immunology 2020Quote: ... Following electrophoretic transfer of proteins onto PVDF membranes (Millipore, IPVH00010), nonspecific binding was blocked by incubation with 5% skim milk ...
-
bioRxiv - Immunology 2020Quote: ... and immunoprecipitated on Sephadex protein A/G beads (Sigma Aldrich, St ...
-
bioRxiv - Biochemistry 2021Quote: ... The fusion protein was purified by Ni-NTA (Novagen, USA) affinity chromatography followed by a Superdex HiLoad 16/600 Superdex 75 pg column (GE Healthcare ...
-
bioRxiv - Physiology 2020Quote: ... Proteins were transferred to polyvinylidene difluoride membrane (Millipore, MA, USA), and the membrane was blocked with Odyssey Blocking Buffer (LI-COR ...
-
bioRxiv - Cell Biology 2021Quote: ... the proteins were transferred to Immobilon-F PVDF membranes (Millipore) at 0.8 mA/cm2 for 2 hr at RT under semi-dry blotting conditions using a discontinuous 3 buffer system (Anode Buffer I ...
-
bioRxiv - Biochemistry 2020Quote: ... proteins in the gels were transferred to PVDF membranes (Millipore). Immunoblots were analyzed using primary antibodies against α4/Pre6 (D ...
-
bioRxiv - Molecular Biology 2021Quote: ... Total protein extracts were made using trichloroacetic acid (TCA, Sigma) precipitation (Knop et al. ...
-
bioRxiv - Molecular Biology 2021Quote: Recombinant proteins were expressed in Escherichia coli RosettaTM (DE3) (Millipore) by induction overnight with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
-
bioRxiv - Microbiology 2020Quote: ... Protein was concentrated using Amicon Ultra-4 30k MWCO (Millipore). 3C protease was added to purified protein in a 1:50 (w:w ...
-
bioRxiv - Neuroscience 2019Quote: ... mouse monoclonal anti-amyloid precursor protein (APP, clone 22C11, Millipore), mouse monoclonal anti-α-Synuclein (Syn-1 ...
-
bioRxiv - Plant Biology 2020Quote: SDS-PAGE and protein gel blotting with PVDF membranes (Millipore) were performed as described (Lauber et al ...
-
bioRxiv - Plant Biology 2021Quote: ... with Magna ChIP™ Protein A Magnetic Beads (Sigma-Aldrich) for Myc-NPR1 ...
-
bioRxiv - Neuroscience 2019Quote: ... Proteins were stained with Brilliant Blue G Colloidal Coomassie (Sigma) and the HDAC6 band of interest was excised ...
-
bioRxiv - Plant Biology 2020Quote: ... and salmon sperm DNA/protein A agarose beads (Millipore, USA) were used for ChIP experiment ...
-
bioRxiv - Molecular Biology 2019Quote: ... Proteins were transferred to a PVDF membrane (ISEQ00010, Merck Millipore) blocked and blotted using the iBind system (SLF1020 ...
-
bioRxiv - Biochemistry 2020Quote: ... Purified 5-HT2C protein immobilized on nickel agarose beads (Sigma) was incubated with a given marker ligand at 2 nM mixed with each test compound (aporphine ligands ...
-
bioRxiv - Cell Biology 2019Quote: ... The resolved proteins were electro-transferred to PVDF membranes (Millipore). The proteins of interest were detected with specific polyclonal or monoclonal antibodies as indicated in the figures ...
-
bioRxiv - Cell Biology 2020Quote: ... and RSV F protein (1:500 dilution, 488 conjugated; Millipore). Cultures were mounted using DAPI mounting medium (Vectashield ...
-
bioRxiv - Pathology 2019Quote: ... The Protein concentration was determined using Bradford reagent (Sigma Aldrich) (Bradford et al. ...
-
bioRxiv - Genetics 2021Quote: ... ChIP-grade protein A+G magnetic beads (Millipore, 16-663) were added and the enriched chromatin was eluted with 150 uL ChIP Elution Buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein expression was then induced with 50 μM copper (Sigma). After 3 hrs of induction ...
-
bioRxiv - Cell Biology 2020Quote: The total protein concentration was determined by Bradford Assay (Sigma) following the manufacturer’s instructions ...
-
bioRxiv - Physiology 2021Quote: ... Protein concentrations were determined by Bicinchoninic acid assay (Sigma-Aldrich) and 30 μg of protein per sample was electrophoresed by SDS PAGE on 4–20% Mini-PROTEAN TGX Stain-Free Precast Gels (Bio-Rad ...
-
bioRxiv - Physiology 2021Quote: ... Proteins were transferred onto a PVDF membrane (0.45μm, IPVH00010, Millipore) and detected using antibodies targeting p16INK4A (Abcam ...
-
bioRxiv - Physiology 2021Quote: ... followed by peroxidase-conjugated Protein G (Sigma P8170; 1:130’000) or peroxidase-conjugated anti-mouse IgG (Fab Specific ...
-
bioRxiv - Biochemistry 2021Quote: ... The column was calibrated using the following protein standards (Sigma); ferritin (12 nm – 440 KDa) ...