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Citations for Millipore Sigma :
2401 - 2450 of 10000+ citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and 1% phosphatase inhibitor cocktail II and III (Sigma-Aldrich, P5726, P0044), the whole procedure was performed at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Rabbit polyclonal antibodies against β-tubulin III (T2200) was from Sigma-Aldrich. Chicken polyclonal antibodies against GFP (ab13970 ...
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bioRxiv - Biochemistry 2022Quote: ... 10 mM βME and protease inhibitor (Protease Inhibitor Cocktail Set III, Sigma)) and sonicated on ice for 1 minute 30 seconds (15s ON ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 1:200 Protease Inhibitor Cocktail Set III (#539134, Merck Millipore)) ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-β-Tubulin III (Tuj1, Sigma reference T2200, 1/1000 dilution), donkey anti-mouse Alexa Fluor 594 (Thermofisher ...
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bioRxiv - Bioengineering 2021Quote: ... Polyethyleneimine (PEI) and Tetrachloroauric (III) acid (HAuCl4.3H2O) were purchased from Sigma-Aldrich. Slide-A-Lyzer dialysis flasks were purchased from ThermoFisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... the glass slides were washed with 1.5% Hellmanex III solution (Sigma Aldrich) and washed with double distilled water ...
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bioRxiv - Neuroscience 2021Quote: ... Mn(III)tetrakis(1-methyl-4-pyridyl)porphyrin (MnTMPyP, 25 µM, Millipore), α-cyano-4-hydroxycinnamate (4-CIN ...
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bioRxiv - Cell Biology 2023Quote: ... and iii) 1 µM rotenone and 1 µM antimycin-A (Sigma-Aldrich). Raw OCR and ECAR measurements were normalized by a Hoechst-based cell count for each well (Cytation1 Cell Imager ...
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bioRxiv - Cell Biology 2024Quote: ... incubated in a solution of 2% cuvette cleaning concentrate (Hellmanex III, Sigma) in ddH2O at 50°C for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... and heparan sulfate proteoglycan with 0.25 U/mL heparanase III (Sigma #H8891). Cells were treated for 1-2 hours at 37°C prior to flow experiments in flow media containing 45 μM BSA and tested using Protocol 1 ...
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bioRxiv - Molecular Biology 2023Quote: chicken Anti-Beta III Tubulin (TUJ1) Antibody (1:400, AB9354 Sigma-Aldrich)
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bioRxiv - Cell Biology 2023Quote: ... and protein analysis of tau and β-III-tubulin (Sigma T-8660) by western blot as described above.
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Necrosis reduction efficacy of subdermal biomaterial mediated oxygen delivery in ischemic skin flapsbioRxiv - Bioengineering 2022Quote: Calcium peroxide (CaO2, FMC Corporation), iron (II, III) oxide (Fe3O4, Sigma-Aldrich), sodium bicarbonate (NaHCO3 ...
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bioRxiv - Plant Biology 2023Quote: ... lanthanum (III) chloride (LaCl3) and coronatine were all from Sigma (Dorset, UK). N-hydroxyl-pipecolic acid (NHP ...
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bioRxiv - Neuroscience 2024Quote: ... (iii) monoclonal mouse anti-NeuN (1:5000; Sigma-Aldrich, Cat. No. MAB377). Sections were rewashed with PBS and incubated with the corresponding secondary antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... complementary DNA was synthesized with SuperScript III First-Strand Synthesis System (Sigma) with random hexamers ...
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bioRxiv - Immunology 2024Quote: ... Mice were intra-gastrically gavaged with 25mg OVA (grade III, Sigma, A5378) in 100μL ...
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bioRxiv - Cancer Biology 2024Quote: ... and phosphatase inhibitor cocktails II and III (#P5726 and #P0044, Sigma-Aldrich). All lysates were freshly prepared and processed with Novex NuPAGE Gel Electrophoresis Systems (Invitrogen) ...
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bioRxiv - Biophysics 2024Quote: ... Both top and bottom coverslips were sonicated in Hellmanex III (Sigma Aldrich) solution (2% V/V in demineralized water ...
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bioRxiv - Molecular Biology 2021Quote: Nuclei from human liver (15 mg) were isolated using with the Nuclei EZ Prep kit (Sigma-Aldrich, #NUC101) and a glass dounce homogenizer (25 strokes with lose pastel and 25 strokes with tight pastel in ice-cold Nuclei EZ Lysis Buffer) ...
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bioRxiv - Neuroscience 2020Quote: Chromatin for ChIP was prepared from immortalized human microglia using the Imprint® Chromatin Immunoprecipitation kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... The genotyping of the DNA extracted from ears of transgenic mice by Extract-N-Amp™ Tissue PCR Kit (Sigma St. Louis, MO) was performed following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse anti-ZO-1 was conjugated beforehand with CF488A using the Mix-n-Stain™ CF™ 488A Antibody Libelling Kit (Sigma, MX488AS100) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Genotypes of the offspring were verified by PCR amplification of genomic ear DNA using the commercially available REDExtract-N-Amp™ Tissue PCR Kit (#R4775; Sigma-Aldrich, Germany) and primers designed to identify the presence or absence of the knock-in allele ...
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bioRxiv - Bioengineering 2024Quote: ... the iBMECs were seeded in the vascular channel at a density of 16 x 106 cells/ml using iBMEC seeding medium (human serum-free endothelial cell medium supplemented with 5% human serum from platelet-poor human plasma (Sigma, Cat.# P2918) and allowed to attach to the membrane overnight (the bottom side of the chip was facing up during this period) ...
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bioRxiv - Developmental Biology 2021Quote: PAs from homozygous a2KO (n = 5) and WT (n = 6) embryos were manually dissected in ice-cold DEPC-treated phosphate-buffered saline (DPBS, Sigma catalog number D8537). Individual PAs were pooled into four samples (WT PA1 ...
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bioRxiv - Cell Biology 2020Quote: ... Then samples were incubated for 1 hour and 15 minutes in 0.16 M N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) (item 03450, Sigma-Aldrich, St. Louis, MO) solution ...
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bioRxiv - Genomics 2020Quote: ... alone or in combination with 5mM N-acetyl-cyteine (NAC) (Sigma-Aldrich, M for 2 hours, alone or in combination with 5mM N-acetyl-cyteine (NAC) (Sigma-Aldrich, A7250). When using NAC ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were reconstituted in 50 μL dry pyridine and 50 μL N-Methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA, Sigma Aldrich, St Louis, MI, USA) silylation agent added ...
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bioRxiv - Plant Biology 2020Quote: ... Then samples were incubated for 1 hour and 15 minutes in 0.16 M N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) (item 03450, Sigma-Aldrich, St. Louis, MO) solution ...
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bioRxiv - Developmental Biology 2022Quote: ... 100ul of ddH2O was added to each well followed by 25ul of 50mg/ml N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (Sigma, St Louis, Missouri) and 25ul of 50mg/ml N-Hydroxysuccinimide (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 10 μM DAPT (N-[N-(3, 5-difluorophenacetyl)-l-alanyl]-s-phenylglycinet-butyl ester) and 4 μM TAT-cre (Millipore, cat. no. SCR508) overnight.
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bioRxiv - Pathology 2023Quote: ... The solvent (n=5) or an FXIa inhibitor (ONO-1600586, 3 µmol/L n=5) and mepacrine (Sigma-Aldrich; final concentration 5 µM) were added to the blood before perfusion ...
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bioRxiv - Cell Biology 2022Quote: ... 5 ml of the pelleted worms were mixed with 50 µl of 50 mM N-ethyl-N-nitrosourea (ENU; Sigma-Aldrich, Munich, Germany) and the suspension was incubated for 4 h at room temperature on a rotating shaker ...
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bioRxiv - Genomics 2023Quote: ... Rats received a single oral gavage administration of freshly prepared 40 mg/kg N-ethyl-N-nitrosourea (ENU) (Sigma Aldrich, St. Louis, MO) dissolved in PBS (Nova-Tech ...
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bioRxiv - Immunology 2024Quote: ... To assess leak by Evan’s blue the lungs were harvested and imaged with a USB desktop digital microscope (Veho) before being placed in 1 mL N,N-dimethylformamide (Sigma #68-12-2) an incubated for 4 days at 37°C to extract the dye from the tissue ...
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bioRxiv - Physiology 2024Quote: Mice were given ad libitum access to drinking water supplemented with 40 mM N-Acetyl-L-cysteine (NAC, N) (Sigma-Aldrich, Cat. #A7250) or water without treatment (vehicle ...
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bioRxiv - Biochemistry 2021Quote: ... The C-terminal domain (CTD) of DRH-3 (residues 940-1108) was cloned into the expression vector pET15b (Novagen), and the resulting clone carries an N-terminal His6 tag and a thrombin cleavage site ...
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bioRxiv - Biochemistry 2022Quote: ... CLP1 contained a C-terminal FLAG-Tag and the sample was purified using ANTI-FLAG M2 Affinity Gel (Sigma), eluted with Pierce 3X FLAG peptide (Thermo ...
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bioRxiv - Microbiology 2023Quote: An N-terminal His-tagged fusion of HcpGA1 was constructed by cloning QR305_02952 into the NdeI–BamHI sites of pET16b (Novagen), and the plasmid was transformed into E ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant SIRV2 gp21 and SiL_0190 with C-terminal His-tag were expressed using Escherichia coli RosettaTM (DE3) cells (Novagen). Protein expression was induced during logarithmic growth through the addition of 0.5mM isopropyl-thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2023Quote: ... with molar ratio of 1:5 (Nucleosome:SUV420H1) or 1:100 (Nucleosome: SUV420H1 C-terminal peptide) in the presence of 40 µM of SAM (Sigma). Samples were incubated on ice for 20 mins ...
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bioRxiv - Microbiology 2024Quote: ... Permeabilized cells were incubated overnight at 4°C with rabbit polyclonal antibody directed against C-terminal NLRP1 (Sigma, #HPA064431) at 1:500 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... which were pre-treated with 0.01% collagen type I (Sigma-Aldrich) in 0.01 M acetic acid solution.
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bioRxiv - Cell Biology 2020Quote: ... and neuraminidase type V at 100 mU/mL (Sigma Aldrich, N2875) (37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... wild type cells crawled under a 0.7% Omnipur agarose (EMD Millipore) gel that was laced with 1 mg/mL of 70,000 MW Rhodamine B isothiocyanate-Dextran (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... FACS-sorted glomerular cells were seeded on collagen Type IV (Sigma) coated 35 mm culture dishes (Sarstedt ...