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Citations for Millipore Sigma :
2401 - 2450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cells were counterstained with DAPI (Sigma D9564) and mounted using VECTASHIELD antifade Mounting Medium (Vector Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... RAW 264.7 and BMDMs were detached using Accutase solution (Sigma-Aldrich: A6964), pelleted and resuspended in media ...
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bioRxiv - Cell Biology 2024Quote: ... or 1mM 13C formate (Sigma-Aldrich: 279412) was added to the regular media ...
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bioRxiv - Cell Biology 2024Quote: Both hippocampal and SCG neurons were fixed using a co-fixative buffer comprising 4% paraformaldehyde (Sigma Aldrich, 158127-3KG), 1X PHEM buffer (containing PIPES ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-talin (8d4; Sigma); PKA-Cα (610980 ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-tubulin (DM1a; Sigma); anti-GAPDH (14C10 ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-vinculin (hVin-1; Sigma); rabbit anti-PKA RIIα (sc909 ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were cultured for a period of 48 hours on 35-mm-diameter culture dishes coated with 0.1 mg/ml poly-D-lysine (Sigma Aldrich, P0899-50MG). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... Chloroform (Sigma-Aldrich: 650498) was used for phase separation ...
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bioRxiv - Immunology 2024Quote: ... and subsequently transduced two times with viral supernatant in the presence of 6 ug/ml polybrene (Sigma) and 50 U/ml IL-2 ...
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bioRxiv - Immunology 2024Quote: ... with 3.5 mL Histopaque 1.077 (Sigma). 7 ml of Histopaque 1.119 was added under the islet phase using a needle ...
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bioRxiv - Immunology 2024Quote: ... The tissue homogenate was then centrifuged at 4°C at 220 x g for 5 minutes and resuspended in 1.1g/mL islet suspension by mixing 7.5 mL Histopaque 1.119 (Sigma) with 3.5 mL Histopaque 1.077 (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10-20% fetal bovine serum (FBS, Sigma) and 100 µg/ml penicillin-streptomycin (p/s ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then stained with 2% (v/v) osmium tetroxide (TAAB) / 1.5% (v/v) potassium ferricyanide (Sigma), incubated in 1% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... Exchange into Durcupan ACM® resin (Sigma) was performed in 50% resin in ethanol ...
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bioRxiv - Cell Biology 2024Quote: ... incubated in 1% (w/v) thiocarbohydrazide (Sigma) with SteadyTemp plate set to 40°C ...
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bioRxiv - Immunology 2024Quote: ... and seeded between 0.5×105 – 1×105 per well in 96 well plate coated with 10 µg/mL Poly-D-lysine hydrobromide (Sigma – Cat. P7886). The medium culture was changed three hours after the seeding and every two days until the seventh day when the cells developed a typical morphology of a mixed neuron culture.
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bioRxiv - Immunology 2024Quote: ... Human PBMC were pre-activated with 30 ng/ml anti-CD3 (OKT3, Miltenyi) and 50 IU/ml IL-2 (Sigma) and subsequently transduced two times with viral supernatant in the presence of 6 ug/ml polybrene (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-α-Tubulin DM1α (Sigma-Aldrich; IF at 1:4000-1:10,000), mouse anti-Aurora A (Cell Signaling Technology 1F8 ...
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bioRxiv - Immunology 2024Quote: ... Lactobacillus johnsonii and Ligilactobacillus murinus were grown anaerobically at 37°C in de Man Rogosa Sharpe (MRS) media (Sigma, MO, USA). Staphylococcus sciuri ...
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bioRxiv - Immunology 2024Quote: ... the pore forming Streptolysin O (S5265-25KU, Sigma) was used to facilitate entry of exogenous NOXA into target cells ...
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bioRxiv - Cell Biology 2024Quote: ... Fluoroshield™ with DAPI (Sigma) was added to the cell suspension before mounting on poly-L-lysine-coated glass slides ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-KMX-1 (IgG2b; Millipore Merck) and then goat anti-mouse IgG2b(Fcγ)-AlexaFluor594 (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... and anti-Beta-actin antibodies (MAB1501, Millipore Sigma) overnight at 4oC ...
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bioRxiv - Immunology 2024Quote: ... mice received a single intraperitoneal (IP) injection of 1mL sterile 3% Brewers Thioglycollate media (Sigma-Aldrich). After 4 days ...
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bioRxiv - Immunology 2024Quote: ... Virus was plaque-purified on Vero CCL81 cells in the presence of 25 μg/mL of cytosine arabinoside (Sigma-Aldrich), and plaques in agarose plugs were amplified on Vero CCL81 cells ...
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bioRxiv - Microbiology 2024Quote: ... and blotted with the anti-FLAG M2 antibody (Sigma) and rabbit anti-StrepII antibody to detect FLAG-tagged OprL and StrepII-tagged OprH ...
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bioRxiv - Microbiology 2024Quote: ... using a 20-cycle protocol with KOD Polymerase Hot Start 2x Mastermix (Millipore Sigma), 20 seconds at 95°C ...
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bioRxiv - Microbiology 2024Quote: ... conjugated to Alexa Flour 647 and Calcofluor White 1:20 (CFW, 1mg/ml, Sigma Aldrich) in PBS for 30 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... A total of 1 μg MBP-fusion protein (in 100 μl of 1% NP40) was incubated with 0.5 μg Avicel microcrystalline cellulose (Sigma-Aldrich) for 3 hours at 4°C with rocking ...
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bioRxiv - Microbiology 2024Quote: Ac trophozoites were grown and maintained in axenic culture at 30°C in T-75 tissue culture flasks in 10 ml ATCC medium 712 (PYG plus additives) with antibiotics (Pen-Strep) (Sigma-Aldrich Corporation, St. Louis, MO) as described previously (21 ...
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bioRxiv - Microbiology 2024Quote: ... We then used PCR with KOD Polymerase 2x Mastermix (Millipore Sigma) to append a T7 promoter sequence ...
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bioRxiv - Microbiology 2024Quote: ... Subsequently these cysts were washed three times with PBS and incubated with secondary anti-rabbit IgG conjugated with Alexa flour 488 (1:300) and Calcofluor White 1:20 (CFW, 1 mg/ml, Sigma Aldrich) for 30 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: The pads are made by mixing the desired combination of growth media and any temperature-stable constituents with 1% w/v agarose powder (Sigma-Aldrich, A9539). The agarose mix is then heated to 80 °C to fully dissolve the agarose powder and cooled to 60 °C before adding temperature-sensitive constituents such as antibiotics ...
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bioRxiv - Microbiology 2024Quote: The antibiotics we used to mix into the pads of the MAP were ampicillin (Sigma-Aldrich 10835242001), carbenicillin (Sigma-Aldrich C1389) ...
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bioRxiv - Microbiology 2024Quote: ... agar dilution method was carried out to determine minimum inhibitory concentration (MIC) for Meropenem (PHR1772-500MG, Pharmaceutical Secondary Standard, SIGMA-ALDRICH) following CLSI guideline (31st edition 2021).17 Quality control was ascertained by using E ...
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bioRxiv - Microbiology 2024Quote: ... using KOD Polymerase Hot Start 2x Mastermix (Sigma). This amplification was performed with 20 cycles total ...
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bioRxiv - Microbiology 2024Quote: ... Novagen KOD Hot Start polymerase was purchased from Millipore Sigma and used for all cloning ...
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bioRxiv - Microbiology 2024Quote: ... Sterile stocks of polymyxin B sulphate (PMB; Cat# 86-40302, Betapharma, Shanghai, China) were prepared in Milli-Q water (Millipore, USA) with filter sterilization using a 0.22-µm syringe filter (Sartorius ...
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bioRxiv - Microbiology 2024Quote: ... with the latter supplemented with 10 mg/L tetracycline hydrochloride (Cat# T7660-5G; Sigma). Following aerobic incubation at 37°C for 18 h ...
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bioRxiv - Microbiology 2024Quote: ... and others from Sigma-Aldrich (St Louis, MO, USA). All stock solutions were prepared in dimethyl sulfoxide (DMSO ...
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bioRxiv - Microbiology 2024Quote: ... Each strain was tested for its susceptibility to d-cycloserine (DCS; Sigma-Aldrich), lurasidone (MedChemExpress) ...
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bioRxiv - Microbiology 2024Quote: ... or Accutase® solution (Sigma-Aldrich, Cat# A6964) and the single cell suspension was harvested for flow cytometry analysis and scRNA-seq.
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bioRxiv - Microbiology 2024Quote: ... Strainers containing fRSS-washed cells were further washed using 500 µL of freshly prepared 6% sodium hypochlorite (Reagent Grade, Sigma) to remove SAB ...
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bioRxiv - Microbiology 2024Quote: The human monocytic cell line U937 (ATCC CRL-1593.2) was obtained from ATCC and primary human peripheral monocytic cells (PBMCs) were purchased from Sigma (HUMANPBMC-0002644). U937 cells were cultured in RPMI medium (Genesee ...
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bioRxiv - Molecular Biology 2024Quote: ... cOmplete Mini protease inhibitor cocktail (Millipore Sigma #11836170001), 10mM NaF ...
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bioRxiv - Microbiology 2024Quote: ... with 25 mM HEPES and (iii) RPMI with 25 mM HEPES and supplemented with 10% human serum (Sigma-H4522). Then ...
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bioRxiv - Microbiology 2024Quote: ... Primary human PBMC were differentiated into macrophages (hMDMs) after culturing for 7 days with RPMI supplemented with 20% heat-inactivated human serum (Sigma-H4522).
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bioRxiv - Molecular Biology 2024Quote: ... Lysate was incubated with anti-FLAG M2 magnetic beads (Millipore Sigma #M8823) for 4 hrs while rotating at 4C then washed 3x with 200µL of lysis buffer using a DynaMag-2 magnet (Invitrogen #12321D) ...
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bioRxiv - Molecular Biology 2024Quote: ... protease inhibitor cocktail (Sigma Merck, P8215), 1mM PMSF ...