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Citations for Millipore Sigma :
2451 - 2500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was determined by BCA assay (Novagen BCA Protein Assay Kit) on a NanoDrop Microvolume Spectrophotometer (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... cOmplete Mini protease inhibitor cocktail (Millipore Sigma #11836170001) 10mM NaF ...
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bioRxiv - Microbiology 2024Quote: ... using 0.5 mm glass beads (Sigma-Aldrich) in 180 μl of an enzymatic lysis buffer containing 20 mM Tris HCl at pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... G3P (10 mM; # 94124; Sigma-Aldrich), antimycin A (90 ng/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... electrophoresed and transferred to a polyvinylidene difluoride membrane (Millipore, Schwalbach, Germany). The membrane was blocked with 5% BSA in Tris-buffered saline with 0.1% Tween 20 ...
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bioRxiv - Molecular Biology 2024Quote: ... in the presence of 50 µM Verapamil (# V4629, Sigma-Aldrich), an efflux pump inhibitor (37 ...
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bioRxiv - Molecular Biology 2024Quote: ... cell lines were generated from parental mountain hare (LT1) and brown hare (LE3) cell lines by lentiviral transduction of shRNA (TRCN0000028617 and TRCN0000028579 MISSION® shRNAs, Sigma-Aldrich) followed by puromycin-resistant marker selection.
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bioRxiv - Molecular Biology 2024Quote: ... antimycin A (90 ng/ml; # A8674; Sigma-Aldrich), ascorbate (700 µM ...
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bioRxiv - Molecular Biology 2024Quote: ... ascorbate (700 µM; # A4034; Sigma-Aldrich), N,N,N′,N′-tetramethyl- p-phenylenediamine (300 µM ...
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bioRxiv - Molecular Biology 2024Quote: ... rotenone (0.15 µM; # R8875; Sigma-Aldrich), glycerol 3- phosphate ...
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bioRxiv - Molecular Biology 2024Quote: ... N,N,N′,N′-tetramethyl- p-phenylenediamine (300 µM, # T7394; Sigma-Aldrich) and potassium cyanide (200 μM ...
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bioRxiv - Molecular Biology 2024Quote: ... and potassium cyanide (200 μM; # 60178; Sigma-Aldrich). Oxygen consumption (pmol.sec−1.ml−1 ...
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bioRxiv - Molecular Biology 2024Quote: ... penicillin-streptomycin (100 U/mL and 100 μg/mL, respectively; Sigma-Aldrich) and 10 ng/mL murine macrophage colony-stimulating factor (M-CSF ...
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bioRxiv - Microbiology 2024Quote: ... and 20 mg/mL lysozyme (Sigma-Aldrich). Homogenates were incubated 1.5 h at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Mitochondrial respiration was assessed by additions of substrates and inhibitors in the following order: pyruvate + glutamate + malate (5 mM each; # P8574, # G5889, # M7397; Sigma-Aldrich), ADP (1 mM ...
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bioRxiv - Molecular Biology 2024Quote: ... pelleted at 250 g for 3 min and resuspended with DPBS (# D8537, Sigma-Aldrich) at 1x106 cells/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 % L-glutamine (# G7513, Sigma-Aldrich), and 1 % penicillin/streptomycin (P/S ...
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bioRxiv - Molecular Biology 2024Quote: ... re-probed with mouse monoclonal anti-Vinculin antibody (1:10000, # V9264, Sigma-Aldrich) followed by peroxidase-conjugated horse anti-mouse IgG (1:10000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Half of the samples were treated with 0.075 µM rotenone (# R8875; Sigma- Aldrich), a mitochondrial Complex I inhibitor ...
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bioRxiv - Biochemistry 2024Quote: ... and phosphate-buffered saline (PBS) were purchased from Sigma-Aldrich. For the IMVs study ...
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bioRxiv - Biochemistry 2024Quote: ... Antibiotics (penicillin and streptomycin) and fatty acid (FA)-free bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St Louis, MO). FBS (Optima ...
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bioRxiv - Microbiology 2024Quote: ... they were diluted 1:100 in fresh 10 ml of the specified growth medium supplemented with 0.2% (w/v) L-arabinose (Sigma) and 1 mM glycerol (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Bound antibodies were probed using 50 μl/well of 1:1,000 of anti-mouse IgG HRP (Sigma–Aldrich) and incubated for an hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Peritoneal macrophages were harvested from the mice 4 days after 1.5 mL of thioglycolate broth (Sigma) was injected ...
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bioRxiv - Biochemistry 2024Quote: ... The following antibodies were acquired from several vendors: mouse monoclonal anti-β-actin antibody (A1978) from Sigma-Aldrich, rabbit anti-mouse pCREB (87G3) ...
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bioRxiv - Biochemistry 2024Quote: ... Cholesterol contents were analyzed with cholesterol quantification assay kit (Sigma).
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bioRxiv - Biochemistry 2024Quote: ... tetrahydrate and L-ascorbic acid were purchased from Acros Organics (part of Sigma Aldrich). Ultrapure water was produced from a Milli-Q unit (Millipore ...
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bioRxiv - Biochemistry 2024Quote: ... cells were collected in growth media after 25 minutes incubation with Accutase (Sigma Aldrich, St Louis, MO) at 37°C in a humidified atmosphere of 95% air and 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... Ultrapure water was produced from a Milli-Q unit (Millipore, conductivity lower than 18 MΩ cm). Rabbit anti-mouse JMJD3 primary antibody was lab-generated as described in1 ...
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bioRxiv - Biochemistry 2024Quote: ... and cholesterol were also supplied by Sigma-Aldrich. Ammonium molybdate (VI ...
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bioRxiv - Biochemistry 2024Quote: ... The protease and phosphatase inhibitor cocktails were purchased from Sigma-Aldrich.
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bioRxiv - Molecular Biology 2024Quote: ... and mouse-anti-RPE65 (MAB5428, Millipore-Sigma, 1:200). Coverslips were washed three times with PBS followed by secondary antibody incubation in blocking buffer for 1 hr at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... pellets were resuspended in 1 ml solubilization buffer (1% SDS, 1x SigmaFast Protease Inhibitor [Sigma-Aldrich], 50 mM HEPES, pH 8.0). Pellet fractions (PF ...
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bioRxiv - Microbiology 2024Quote: ... for 3 days prior to fungal infection at 200 mg/kg/day6 or sertraline hydrochloride (Sigma) in saline ...
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bioRxiv - Microbiology 2024Quote: ... Mycelial balls were harvested using Miracloth (EMD Millipore Corp., USA) and rinsed with ultrapure Milli-Q® water (Merck Millipore ...
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bioRxiv - Microbiology 2024Quote: ... The eluted fractions were concentrated by filter (Amicon Ultracel-30K, Merk Millipore) and dialyzed against TNE buffer (20mM Tris-HCl pH8.0 ...
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bioRxiv - Microbiology 2024Quote: ... The transformants were selected on solid LB (Sigma, Fisher Scientific) medium (agar 15% ...
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bioRxiv - Microbiology 2024Quote: ... Unbound antibodies were eliminated by washing with 50 mL maleic acid buffer + 150 µL Tween 20 (Sigma Aldrich) for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... pNPP (p-nitrophenyl phosphate; Sigma-Aldrich) was used as a soluble substrate ...
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bioRxiv - Microbiology 2024Quote: ... mycelia were harvested using Miracloth (EMD Millipore Corp., USA), rinsed with sterile water and same amounts of mycelium were transferred to 2-mL screw cap tubes for RNA isolation ...
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bioRxiv - Microbiology 2024Quote: ... The PCR reaction mixture consisted of Kappa Probe Fast qPCR Master Mix (2X) Kit (Sigma-Aldrich), 0.75 μL of primers and 2.5 μL of RNA in a final volume of 10 μL reaction ...
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bioRxiv - Microbiology 2024Quote: ... Mice were anesthetized with 0.5 ml intraperitoneal injections of tribromoethanol (TBE) solution (250 mg/kg; Sigma-Aldrich); dorsum of mice was shaved and a small incision made aseptically and a subcutaneous tunnel was created allowing for insertion of up to 5 pieces of pre-inoculated catheters (Fig ...
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bioRxiv - Microbiology 2024Quote: ... we added fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG (Sigma, USA) at a dilution of 1:200 and incubated it at 37°C for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... nitrocefin (EMD Millipore), and its beta-lactamase activator ...
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bioRxiv - Cell Biology 2024Quote: ... and 18 mm coverslips were activated using 3-aminopropyltrimethoxysilane (Sigma Aldrich) for 5 min and then for 30 min with 0.5% glutaraldehyde (Electron Microscopy Sciences ...
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bioRxiv - Microbiology 2024Quote: ... The spleen cells were separated and fused with SP2/0 myeloma cells three days later by 50% (w/v) PEG4000 (Sigma-Aldrich, USA). PCV4-VLP-ELISA screened the antibodies in supernatants of hybridoma ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 mg/mL lysozyme (Sigma) and protease inhibitor cocktail (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... A 5 nm colloidal gold-conjugated antibody (diluted 1:100, Sigma, USA) was applied to the grid ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% heat inactivated FBS (Sigma) at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2024Quote: ... was used as second antibody and proteins on the membrane were visualized using BCIP/NBT substrate (Sigma). When deer plasma specimens were used as primary antibody ...