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Citations for Millipore Sigma :
2351 - 2400 of 4285 citations for Human KIRREL shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... A final dilution of ∼105 CFU was mixed with human serum (Sigma-Aldrich, H6914 from male AB clotted whole blood ...
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bioRxiv - Immunology 2024Quote: ... and 10% human serum (heat-inactivated, sterile-filtered, male AB plasma; Sigma-Aldrich). Adaptive NK cell-positive donors were identified by anal-ysis of NKG2C (FAB138P or FAB138A antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... and human dermal fibroblasts were treated with 20 μM CCCP (Sigma-Aldrich, C2759), 5 μM valinomycin (Cayman Chemicals ...
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bioRxiv - Microbiology 2024Quote: ... which was the concentration used for the human IgG negative control (Sigma, I2511) in subsequent experiments ...
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bioRxiv - Microbiology 2024Quote: ... MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Customised 7-plex Panel (Merck Millipore) was used to quantify cytokine levels.
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bioRxiv - Cell Biology 2024Quote: ... Dendritic cells were either cultured with 20 μg/ml human insulin (Sigma-Aldrich) to obtain immature DCs (iDCs ...
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bioRxiv - Molecular Biology 2024Quote: All human cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich) supplemented with 10% fetal calf serum (FCS) ...
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bioRxiv - Bioengineering 2024Quote: ... and a mouse monoclonal anti-human fibrin and fibrinogen (F9902, Sigma-Aldrich, USA) were incubated inside the flow chambers overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... and then incubated with rabbit anti-human IgM and Hoechst 33258 dye (Sigma) to visualize nuclei ...
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bioRxiv - Biochemistry 2024Quote: ... human Trx1 was concentrated to 2.5 mM using the Stirred Ultrafiltration Cell (Millipore) and buffered in PBS using PD-10 columns (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... primary human PBMCs were isolated by density gradient sedimentation in Histopaque® (Sigma) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2021Quote: ... The plasmid was extracted using a mini-prep extraction kit (Sigma-Aldrich), and the introduced mutations were confirmed by DNA sequencing.
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bioRxiv - Cell Biology 2020Quote: ... These plasmids were co-transformed into the Rosetta2 strain of E.coli (Novagen). Multiple liters of culture were grown in terrific broth for 8-12h at 37°C (until the OD600 ∼2-3) ...
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bioRxiv - Microbiology 2019Quote: ... the plasmids were transformed into Escherichia coli Rosetta pLys-S cells (Novagen) for expression of the protein.
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bioRxiv - Microbiology 2019Quote: ... These plasmids were co-transfected into 293T cells using XtremeGene (Sigma-Aldrich) transfection reagent according to the manufacturer’s recommended procedure ...
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bioRxiv - Neuroscience 2019Quote: ... plasmids were mixed with 2 mg/ml Fast Green (Millipore Sigma, F7252). In all conditions ...
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bioRxiv - Biochemistry 2021Quote: Plasmids were transformed into chemically competent Escherichia coli BL21(DE3) cells (Novagen) and used to inoculate TB medium supplemented with 100 µg/mL of ampicillin or 50 µg/mL of kanamycin for pGEX-6P-1 or pET28b vectors ...
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bioRxiv - Biochemistry 2021Quote: ... Amplified gene drmA was cloned in plasmid pACYCDuet-1 (Sigma-Aldrich: 71147), resulting in a non-tagged protein product ...
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bioRxiv - Cell Biology 2020Quote: ... pLKO.1 puro plasmid containing scrambled sequence was used (Sigma SHC016-1EA). pLKO.1 constructs were co-transfected with psPAX2 (Addgene 12260 ...
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bioRxiv - Biochemistry 2022Quote: Plasmids for sgRNA expression were cloned by annealing synthetic oligonucleotides (Sigma-Aldrich) and ligating them into the appropriate vector ...
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bioRxiv - Microbiology 2022Quote: The C1-07020 gene was cloned into the plasmid pETDuet-1 (Novagen) and then transformed into E ...
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bioRxiv - Bioengineering 2022Quote: ... DogCatcher-NANP18 and SpyCatcher were cloned into expression plasmid pET45(+) (EMD Millipore) for protein production in BL21(DE3 ...
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bioRxiv - Biochemistry 2021Quote: ... Retromer plasmids were co-transformed into BL21(DE3) Rosetta2 pLysS cells (Millipore). Cells were grown to OD600 between 0.8-1.0 and induced for 16-20 hours at 20°C with 0.4 mM IPTG ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmid pET28a(+) was purchased from Novagen (EMD Biosciences, San Diego, CA, USA). Preparation of plasmid pACYCcamAB for coexpression of putidaredoxin reductase (CamA or PdR ...
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bioRxiv - Molecular Biology 2022Quote: ... All plasmids were prepared in Stbl3 or DH5α using MiniPrep (Sigma-Aldrich) or MidiPrep (Macherey-Nagel ...
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bioRxiv - Biochemistry 2019Quote: ... the pRSFMBP vector was constructed based on the plasmid pRSFDuetTM-1 (NOVAGEN) with the replacement of the coding sequence for the His-tag by that of MBP (Maltose Binding Protein ...
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bioRxiv - Neuroscience 2019Quote: ... DNA plasmids mixed in saline (PBS) solution and 0.025% Fast Green (Sigma) were injected through the uterine wall into the lateral ventricle of each embryo ...
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bioRxiv - Developmental Biology 2020Quote: ... Plasmids were transfected in respective iFNP cells cultured in DMEM (Sigma #5796) supplemented with 10% calf serum with 50 U/mL each penicillin and streptomycin ...
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bioRxiv - Synthetic Biology 2019Quote: ... The digested plasmids were checked by agarose gel electrophoresis (1% agarose (Sigma), 1xTris-boric acid EDTA buffer ...
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bioRxiv - Microbiology 2020Quote: ... The resulting plasmid (pBEL1307) was transformed into Rosetta 2 (DE3) cells (Novagen). For expression ...
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bioRxiv - Pathology 2021Quote: ... The lentiviral plasmids (LV01) and lentivirus production were obtained from Sigma-Aldrich. Cells were sorted for GFP expression by flow cytometry and maintained in puromycin (3μg/mL) ...
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bioRxiv - Biochemistry 2021Quote: ... The digested PCR product was cloned into a pET-52b(+) plasmid (Novagen) in frame with an N-terminal Strep-tag II obtaining the B35SSB expression vector pET52b::B35SSB ...
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bioRxiv - Genetics 2021Quote: ... coli using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich, St. Louis, MO) or the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: HEK293T cells transfected with indicated plasmids were lysed using SDC (D6750, Sigma) buffer (4% SDS ...
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bioRxiv - Cell Biology 2021Quote: ... plasmids were transformed into BL21(DE3)pLysE bacteria (Sigma-Aldrich, CMC0015-20X40UL) and grown in TB supplemented with 50 µg/mL kanamycin and 33 µg/mL chloramphenicol at 37 °C until an OD600 of 0.4 was reached ...
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bioRxiv - Developmental Biology 2022Quote: ... Plasmid DNA was mixed with Fast Green FCF (final 0.05%; F7252, Sigma) and injected at 2μg/μl into the cochlear duct using the Wiretroll plunger system (53507-426 ...
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bioRxiv - Genetics 2022Quote: ... Transgenic cells were selected with puromycin (sgRNA plasmids) (1 ng/μl, Sigma) or hygromycin (FIREWach plasmids ...
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bioRxiv - Microbiology 2022Quote: ... NS5 coding sequences were subsequently transferred into expression plasmid pET-15b (Novagen) using standard molecular biology procedures (Supplementary Figure S1) ...
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bioRxiv - Neuroscience 2023Quote: ... and 32.5µg gag-pol packaging plasmid (pCMVΔR8.74) using 10 mM polyethylenimine (Sigma) in OptiMEM for three hours (48) ...
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bioRxiv - Plant Biology 2023Quote: ... ATG3 coding regions were cloned into the pET28a (+) plasmid (Novagen, 69864-3) for expression of the corresponding His-tagged protein ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids were transformed into Escherichia coli BL21 Tuner (DE3) strain (Sigma-Aldrich) or Rossetta DE3 (Novagen ...
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bioRxiv - Microbiology 2023Quote: ... or control plasmid expressing 3xFLAG-tagged TRIM25 through X-tremeGENE9 (Millipore Sigma). Twenty-four hours later ...
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bioRxiv - Immunology 2023Quote: ... 293T cells were transfected with premixed plasmids and PEI (MW25000Da, Sigma, USA) and incubated for 72 h ...
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bioRxiv - Cell Biology 2023Quote: ... transfection agent with 3rd generation packaging plasmids and either shScr (SHC332; Sigma), shYAP (addgene #42541) ...
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bioRxiv - Microbiology 2023Quote: ... This plasmid carries the pRSF1030 replicon from RSFDuet™ (Novagen, Merck Millipore), araC and the araBAD promoter from pBAD/His (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... This plasmid carries the pRSF1030 replicon from RSFDuet™ (Novagen, Merck Millipore), araC and the araBAD promoter from pBAD/His (Invitrogen) ...
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bioRxiv - Synthetic Biology 2023Quote: ... lac operon amplicon was amplified from the pETDuet-1 plasmid (Novagen 71146) and cloned into the pBBR1-MCS1 plasmid to construct the pBBR1-MCS1-plac plasmid ...
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bioRxiv - Cancer Biology 2024Quote: calf thymus DNA and supercoiled plasmid pUC19 DNA were obtained from Sigma Chemical Company ...
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bioRxiv - Microbiology 2020Quote: ... bacteria were incubated with 0.5 µg/mL of chicken anti-human C1qA (Sigma-Aldrich), followed by incubation with PE-conjugated donkey F(ab’)2 anti-chicken (Jackson ImmunoResearch) ...
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bioRxiv - Immunology 2021Quote: ... 0.4µg pCMV-VSV-G and 1.6µg of a lentiviral vector (for human shSUV39H1, Sigma, TRCN0000157251 ...