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Citations for Millipore Sigma :
2201 - 2250 of 4285 citations for Human KIRREL shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Plasmids were transformed into chemically competent Escherichia coli Rosetta cells (Novagen) and grown at 37°C in Super Broth with kanamycin (30mg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid was electroporated into Rosetta™ 2 cells (Millipore Sigma) for protein expression ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting plasmid was transformed into Rosetta (DE3) pLys (Novagen, 70956) and a 100 ml overnight culture was grown overnight at 37°C in LB medium supplemented with kanamycin (50 ug/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... envelope plasmid and 130 μL 1 μg / mL polyethylenimine (Sigma-Aldrich) in 450 μL Opti-MEM media (Lonza) ...
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bioRxiv - Biophysics 2023Quote: ... was cloned using Gibson assembly 40 into plasmid pET15b (Novagen/Merck), which was digested with NcoI ...
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bioRxiv - Microbiology 2023Quote: ... host genome and plasmid DNA were digested with DNase I (Sigma) before releasing the viral DNA from the particles ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA plasmids were complexed with polyethyleneimine (PEI; #408727, Sigma-Aldrich, USA) for 15 min and added dropwise to the cells ...
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bioRxiv - Biochemistry 2023Quote: ... coli DH5a into pACYCDuet-1 bicistronic plasmid (Sigma-Aldrich, Burlington, MA), and the resulting co-expression plasmid was introduced to E coli ...
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bioRxiv - Genetics 2023Quote: ... The plasmids were transformed into Rosetta(DE3)pLysS cells (Sigma-Aldrich). Protein expression was induced with isopropyl b-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Molecular Biology 2023Quote: ... The respective plasmid was transformed into BL21 (DE3) competent cells (Novagen), grown in LB broth or M9 medium supplemented with 100 mg/ml ampicillin and induced for expression with a final concentration of 0.5 mM isopropyl-D-1-thiogalactopyranoside at an optical density (600 nm ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid was transformed into Rosetta2(DE3) pLysS competent cells (EMD Millipore) and plated on LB agar plates containing 100μg/mL ampicillin and 34μg/mL chloramphenicol ...
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bioRxiv - Neuroscience 2023Quote: ... and 500 μg of pXX6 plasmid mixed with polyethylenimine (Sigma-Aldrich). The UPV-UAB generated a pAAV plasmid containing the ITRs of the AAV2 genome ...
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bioRxiv - Genetics 2023Quote: Plasmid transfection was carried out with XtremeGENE HP (Millipore Sigma, 6366244001) according to manufacturer protocol at a reagent/DNA ratio of 2:1 in serum free media ...
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bioRxiv - Cell Biology 2020Quote: A2780 human ovarian cancer cells were maintained in RPMI-1640 medium (Sigma-Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-human α-smooth muscle actin (Cy3 conjugated, cat. #C6198, Sigma Aldrich, 1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then suspended in veronal buffer and human serum (Sigma-Aldrich, H4522) (or heat-inactivated for 30min at 56°C) ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteases were obtained from the following vendors: human cathepsin L (Millipore Sigma, Athens Research and Technology ...
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bioRxiv - Molecular Biology 2021Quote: The gene of human GAPDH was cloned into pET-28a(+) expression vector (Novagen) with an N-terminal His-tag followed by the Tobacco Etch Virus (TEV ...
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bioRxiv - Biochemistry 2022Quote: ... or −3 human cDNA into a p3XFLAG-CMV-14 expression vector (Sigma, E7908). Then 3XFLAG-PRLs were cloned into pLenti-CMV-puro (Addgene 17452 ...
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bioRxiv - Cell Biology 2022Quote: ... dried and incubated with 10 μg/mL of IgG from human serum (Sigma) in PBS for 1 hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... were injected with 400 IU human chorionic gonadotropin (hCG; Sigma-Aldrich CG10; RRID:SCR_018232).
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bioRxiv - Molecular Biology 2021Quote: ... bleomycin and human activating FAS (anti-FAS) antibody were purchased from Millipore Sigma. PGE2 ...
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bioRxiv - Immunology 2022Quote: ... media was replenished with RPMI containing 5% type AB human serum (Sigma-Aldrich). For single-round experiments with VSV-G-pseudotyped viruses ...
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bioRxiv - Systems Biology 2019Quote: ... The human plasma reference standard NIST SRM 1950 was obtained from Sigma Aldrich. Human cancer cells (HeLa S3 ...
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bioRxiv - Cell Biology 2020Quote: ... in acetic acid (0.02 N) or human plasma fibronectin (5 µg/mL; Millipore) in PBS overnight at 4°C and rinsed with imaging medium to remove the coating solution ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were blocked with human IgG (1.25 mg/mL, 15 min; Sigma Aldrich). Surface staining of unfixed/unpermeabilized cells employed the following antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... PINK1 knockdown was performed using MISSION® esiRNA human PINK1 (EHU057101, Sigma Aldrich). Mff knockdown (SI Fig ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Sections were incubated with rabbit anti-human-IgG (1:100, Sigma, SAB3701291, IgG) and with rabbit anti-human IgM (1:250 ...
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bioRxiv - Immunology 2019Quote: ... anti-human C5aR or nonpeptide C5aR antagonist W-54011 (5 μM, Merck Millipore)(25 ...
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bioRxiv - Cell Biology 2019Quote: ... Staining for murine and human MSCs was performed using Oil Red O (Sigma) stain for adipogenesis ...
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bioRxiv - Cell Biology 2020Quote: The human ovarian cancer cell lines COV318 (purchased from ECACC, via Sigma-Aldrich) and SKOV3 (courtesy of Dr Florence Delie ...
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bioRxiv - Cancer Biology 2019Quote: ... human breast cancer were detached using Trypsin (when grown as monolayer, Sigma-Aldrich) or pelleted and dissociated using StemPro Accutase (when grown as spheres ...
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bioRxiv - Immunology 2021Quote: ... Total IgA purified from human serum was used as a standard (Sigma-Aldrich). To generate the IgA standard curve anti-human IgA capture antibodies ...
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bioRxiv - Immunology 2021Quote: ... and then overlaid with complete DMEM containing 10 μg/mL human IgG (Sigma). 48 hours post infection ...
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bioRxiv - Microbiology 2021Quote: ... Primary human monocytes were treated with 50 ng/ml M-CSF (Sigma-Aldrich) in complete RPMI for a week before infection ...
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bioRxiv - Biophysics 2020Quote: The recombinant human Mdm2 (1-125 aa) were cloned into pET11a vector (Novagen) and expressed in E ...
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bioRxiv - Bioengineering 2021Quote: ... and human β2m were purchased from IDT and cloned into pET3a vectors (Novagen). H2-Db and H2-Kb genes were engineered with a C-terminal cysteine by site-directed mutagenesis (NEB) ...
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bioRxiv - Bioengineering 2021Quote: Human serum albumin (> 97% lyophilized) was purchased from Sigma Aldrich (Oakville, ON, Canada). Glutaraldehyde (25% aq ...
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bioRxiv - Bioengineering 2021Quote: ... Serum pramlintide concentrations were quantified using a human amylin ELISA kit (Millipore Sigma). Serum lispro concentrations were quantified using Northern Lights Mercodia Lispro NL-ELISA ...
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bioRxiv - Bioengineering 2021Quote: ... 100 ng/mL Follicle Stimulating Hormone from human pituitary (FSH, Millipore Sigma, F4021), 10 ng/mL LH ...
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bioRxiv - Biochemistry 2021Quote: FFPE human tonsils were deparaffinized in xylene substitute (Sigma-Aldrich, St. Louis, MO), rehydrated in a graded ethanol series and stained for 10 minutes with Atto 465-carboxcylic acid (8 uM ...
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bioRxiv - Biophysics 2021Quote: ... lyophilized rabbit muscle aldolase and lyophilized human hemoglobin were purchased from Sigma Aldrich. The catalase was diluted to a final concentration of 2.3 mg ml-1 in the buffer of PBS (pH 6.5) ...
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bioRxiv - Neuroscience 2021Quote: ... were injected with 400 IU human chorionic gonadotropin (hCG; Sigma-Aldrich CG10; RRID:SCR_018232). The following day ...
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bioRxiv - Cancer Biology 2021Quote: siRNAs targeting human SUV39H1/H2 and negative control siRNAs72 were from Sigma-Aldrich. Transfections were performed using Lipofectamine RNAiMax (Thermo Fisher ...
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bioRxiv - Biophysics 2021Quote: ... pre-coated with 30 μg/ml of fibronectin from human plasma (Sigma-Aldrich) and nanodiamonds (100 nm ...
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bioRxiv - Biophysics 2021Quote: His-tagged human PCNA protein was purchased from Sigma Aldrich (catalogue number SRP5117), at a concentration of 6.6 μM ...
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bioRxiv - Neuroscience 2020Quote: ... Plates were incubated with anti-human Fab specific-HRP conjugated secondary antibody (Sigma) for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... 100 ng/ml cholera toxin and 10 μg/ml recombinant human insulin (Sigma). MCF10A I-PpoI cells and MCF10A AsiSI cells were previously described (Harding et al. ...
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bioRxiv - Bioengineering 2021Quote: ... a standard curve was obtained by diluting human Hgb powder (Sigma-Aldrich, USA) in human plasma (Cambridge Bioscience ...
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bioRxiv - Microbiology 2021Quote: Human umbilical VECs (Cell Application 200-05n) were cultured on fibronectin (Sigma 10838039001)-coated glass slides or polyacrylamide (PA ...