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Citations for Millipore Sigma :
2351 - 2400 of 10000+ citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Following a 15 min entry phase under standard cell culture conditions the cells were fixed and the nuclei stained for 1 h at RT by addition of 33 μl 16% PFA and 4 μg / ml Hoechst 33342 (Sigma-Aldrich, St. Louis, USA) in PBS ...
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bioRxiv - Genetics 2021Quote: ... coli OP50 supplemented with or without vitamin B12 (methylcobalamin or MeCbl, Sigma, Cat #13422-55-4; adenosylcobalamin or AdoCbl, Sigma, Cat #13870-90-1) and propionic acid (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated with one or more of the following primary antibodies overnight at 4°C: AGO2 1:1500 dilution (Sigma-Aldrich SAB4200085 Lot#087M4841V), GW182 1:10,000 dilution (Bethyl A302-329A-T Lot#A302-329-T-2) ...
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bioRxiv - Neuroscience 2020Quote: Mice were humanely killed by pentobarbital overdose (>100 mg·kg−1) and transcardially perfused with paraformaldehyde solution (4% PFA; Sigma-Aldrich, St Louis, MO, USA). The head was removed and postfixed in PFA (4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were then washed three times in 1× PBST prior to mounting on glass slides in glycerol with Mowiol 4-88 anti-fade agent (EMD Millipore Corp., Billerica, MA). When required ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... The plates were washed using ELISA washing solution followed by addition of substrate (4-Nitrophenyl phosphate disodium salt hexahydrate, Sigma-Aldrich, 1 mg/ml), 150 µl/well ...
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bioRxiv - Neuroscience 2020Quote: ... and transcardially perfused with 1 M phosphate buffer saline (PBS, 50 ml) followed by 4% paraformaldehyde (PFA, 100 ml, Sigma, St. Louis, MO, USA). Brains were removed and post-fixed in 4% PFA (50 ml ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were stained with 1 μg/ml 4′,6-Diamidino-2-phenylindole dihydrochloride in distilled water (D8417; Sigma-Aldrich, St. Louis, MO, USA), cover-slipped ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... airway mechanics were measured by challenging pigs to increasing concentrations of methacholine hydrochloride (MeCh) (0, 0.5, 1, 2, 4, 8, 16, and 32 mg/mL) (Sigma-Aldrich, St. Louis, MO, USA) via an Aeroneb™ nebulizer (Aerogen Ltd ...
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bioRxiv - Genomics 2022Quote: ... slides were incubated in 1% Kodak PhotoFlo at RT for 1 minute and counterstained with 0.5 mg/mL DAPI (4′,6-diamidino-2-phenylindole, Sigma-Aldrich, St. Louis, MO, USA) in antifade based on DABCO (1,4-diazabicyclo(2.2.2)-octane ...
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bioRxiv - Neuroscience 2021Quote: ... washed twice in PBS and incubated overnight at 4°C in primary anti-KCC2 raised in rabbit (1:1000, Millipore/Upstate, Cat. #07–432), anti-CGRP raised in mouse (1:5000 ...
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bioRxiv - Physiology 2022Quote: ... membranes were sequentially incubated with primary antibody (a 1:1000 dilution) for 16 hours at 4 °C and then biotin-conjugated goat anti-rabbit IgG (Millipore-Sigma/Chemicon, Oakville, ON, Canada) secondary antibody (1:10,000 dilution ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed and surface-stained for 30 min at 4°C in the dark and fixed with 1% of paraformaldehyde (Sigma-Aldrich, St. Louis, MO). LOQ and % responders are calculated as described above.
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bioRxiv - Immunology 2022Quote: ... cells were washed and surface stained for 30 min at 4°C in the dark and fixed with 1% of paraformaldehyde (Sigma-Aldrich, St. Louis, MO). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... catalog # BP151-500) in PBS for 1 hour followed by overnight incubation at 4°C with the γ-H2AX antibody (Millipore Sigma, catalog # 05636I) using a 1:150 dilution and the H3K9me3 antibody (Millipore Sigma ...
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bioRxiv - Pathology 2020Quote: ... and 0.1% Triton-X 100 in PBS) and stained overnight at 4° C with antibodies against PRG2 (1:1000 dilution, rabbit; Sigma-Aldrich cat. no. HPA038515), HLA-G (1:10,000 dilution ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were incubated in primary antibody overnight at 4° C (chick α-GFP, Abcam, Cat# AB13970, Lot# GF305729-1; mouse α- γ-tubulin, Sigma-Aldrich, Cat# T6557 ...
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bioRxiv - Developmental Biology 2020Quote: ... Standard IF staining was carried out by incubation of tissue sections with primary antibodies overnight at 4 °C at the following dilutions: mouse anti-acetylated tubulin (Sigma cat. # T7451; 1:1000), mouse anti-RAB11 (BD Transduction Laboratories cat ...
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bioRxiv - Genomics 2021Quote: ... Serum samples obtained from vaccinated and non-vaccinated individuals were stored at −20°C until tested treated with receptor destroying enzyme (RDE) (Sigma C8772, diluted 1:4), for 16 h prior to heat inactivation (30 min ...
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bioRxiv - Developmental Biology 2022Quote: Dissected intact embryos were fixed for 1 hr at room temperature with 4% paraformaldehyde (SantaCruz, Cat No. sc281692) in PBS (Sigma-Aldrich, Cat No. P4417). The embryos were then rinsed twice in PBST-0.1% (PBS with 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The reaction was stopped by the addition of 28 μl of a stop reaction mixture: 3 μl of the reducing reagent (mixture of 0.2 g 1-amino-2-naphtol-4-sulfonic acid (Millipore-Sigma, Burlington, MA, USA, #08751) with 0.2 g sodium bisulfite (Millipore-Sigma ...
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bioRxiv - Plant Biology 2023Quote: ... in a petri dish and then placed in 12-well plates containing 90% liquid MS medium (1/4 strength) and 10% polyethylene glycol (PEG, MW 8000, SIGMA, cat # 25322-68-3) (p/v ...
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bioRxiv - Neuroscience 2023Quote: Mice were humanely killed by pentobarbital overdose (>100 mg·kg−1) and transcardially perfused with paraformaldehyde solution (4% PFA; Sigma-Aldrich, St Louis, MO, USA). The head was removed and postfixed in PFA (4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Sections were then incubated with the following primary antibodies diluted 1:200 in PBS overnight at 4°C: β-spectrin non-erythrocyte (hpa012685, Sigma, St. Louis, MO), collagen type II (ab34712 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Membranes were incubated overnight at 4°C with primary antibodies probing Spectrin and enzyme-mediated degradation products (mouse anti-Spectrin, 1:1000, Millipore Sigma, Cat. No. MAB1622) and early growth response protein-1 (mouse anti-Egr-1 ...
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bioRxiv - Physiology 2024Quote: ... Nuclear stains used in this study were 4’,6-diamidino-2-phenylindole (DAPI; final concentration 1 μg/ml Sigma-Aldrich, St. Louis, MO, USA). F-Actin was stained with Alexa Fluor 568 phalloidin (1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were incubated in DAPI for 1min washed twice in PBS and stored at 4°C in 1:50 Penicillin-Streptomycin (Thermo Fischer Scientific 5000U/mL) and Gentamicin solution (Sigma Aldrich, 10mg/mL solution) in PBS until imaged ...
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bioRxiv - Molecular Biology 2023Quote: ... One membrane was used to probe for HURP (HURP antibody described in this paper, 4 μg/mL) and γ-tubulin (Sigma: T6557, 1:1000). The second membrane was used to probe for XMAP215 (Abcam ...
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bioRxiv - Physiology 2024Quote: ... Tissues/organs were then permeabilized by incubating on a rocker for 1 h at RT in 4% Triton X-100 (Sigma Aldrich, Oakville, ON, Canada), 10% normal sheep serum (NSS ...
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bioRxiv - Microbiology 2022Quote: ... Neurons were stained with the following primary antibodies overnight at 4°C: mouse monoclonal anti-β-III-Tubulin (Tuj1, 1:1000; Millipore cat. No. MAB5564) and rabbit polyclonal anti-SARS-CoV-2 (COVID-19 ...
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bioRxiv - Physiology 2023Quote: ... Free-floating brain sections were incubated at 4°C overnight with mouse anti-Glial fibrillary acidic protein (GFAP, 1:1000, Sigma-Aldrich, Saint-Louis, USA) or mCherry (ab125096 ...
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bioRxiv - Genomics 2023Quote: ... Primary antibodies were applied overnight at 4°C and included TH (Pel-Freez Biologicals #P40101 and Merck Millipore #AB9702, both at 1:750 dilution), MAP2 (Santa Cruz #sc-74421 ...
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bioRxiv - Neuroscience 2024Quote: ... Unbound antibody was washed with PBS++ including 4’,6-diamidino-2-phenylindole for initialization (DAPI; 1:10 000, Sigma Aldrich, St. Louis, MS, USA). Where needed ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were then incubated with slight agitation (40 rotations/min) overnight at 4°C with a rat anti-muscarinic acetylcholine receptor M2 (M2 AChR) primary antibody (1:500, Sigma-Aldrich, reference number MAB367) in the same blocking solution ...
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bioRxiv - Molecular Biology 2024Quote: ... Guts were incubated overnight at 4°C with a primary anti-catalase antibody (C0979, Sigma; 1:250 in 5% w/v BSA in PBS). After three washes with PBS ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were fixed in 4% paraformaldehyde and immunolabeled with a neuronal-specific anti-β-III tubulin antibody (1:150 dilution; Millipore, Burlington, MA, #MAB1637), followed by a goat anti-mouse Alexa Fluor 488 secondary antibody (1:300 dilution ...
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bioRxiv - Biophysics 2020Quote: ... a gallium concentration standard (1 mg mL-1, 20 μL; Sigma-Aldrich) was added ...
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bioRxiv - Biophysics 2020Quote: ... or anti-β-tubulin (Sigma, T7816, 1 mg ml−1 in PBS) antibody solution for 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 mM sodium orthovanadate) and 1% N-ocetylglucoside (NOG, Sigma-Aldrich) for 10 min on ice ...
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bioRxiv - Microbiology 2020Quote: ... diluted 1:500 in MAXblock medium with 1 μg/ml DAPI (Sigma). Slides were mounted with Fluoroshield (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... at 1:1000 dilution and monoclonal antibody B-5-1-2 (Sigma) at 1:1000 dilution to detect α-tubulin as loading control.
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bioRxiv - Cell Biology 2020Quote: ... anti-FLAG (Sigma, Cat#F3165, 1/1000; Sigma, Cat#F7425, 1/1000), anti-Myc (MBL ...
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bioRxiv - Developmental Biology 2020Quote: ... were fixed in 1 ml of PBS containing 1% formaldehyde (Sigma-Aldrich) and 0.5% Igepal CA-630 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... beta-actin(AC-15, 1:3000), FLAG(F1804, 1:1000) from Sigma; PARP (9542s) ...
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bioRxiv - Cell Biology 2022Quote: INS-1 cells and INS-1 823/3 cells (Sigma-Aldrich, scc208) were cultured in RPMI-1640 medium ...
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bioRxiv - Cell Biology 2022Quote: ... 1:1000 mouse-anti-α-SMA (Millipore; Burlington, MA; Clone ASM-1), 1:2000 mouse-anti-β-actin (Cell Signaling Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... 1:100 anti-IdU (Bencton Dickinson) and 1:3000 anti-ssDNA (Millipore) for one hour ...
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bioRxiv - Genetics 2020Quote: 1-5×106 cells were fixed in 1 ml TRI-reagent (Sigma), snap frozen and stored at −80°C for less than one year ...
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bioRxiv - Cell Biology 2020Quote: ... 1% Triton X100] containing 1% protease and phosphatase inhibitor cocktails (Sigma Aldrich) on ice ...
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bioRxiv - Neuroscience 2020Quote: ... The primary antibodies (PV; 1:1000, Swant CS56; 1:100, Sigma-Aldrich; Biotin-WFA ...