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Citations for Millipore Sigma :
2301 - 2350 of 10000+ citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... The blocking solution was aspirated and replaced with the same solution with primary antibodies (MAP2-Ck, Abcam ab5392, 1:500; Nanog-Gt, R&D AF1997, 1:200; TRA-1-60 IgM-Ms, Millipore MAB4360 ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were then blocked with 5% normal donkey serum in 1X PBS for one hour before overnight incubation in primary antibodies (Foxf1, goat, R&D, 1:300; Sox9, mouse, Invitrogen, 1:200; Sox9, rabbit, Millipore, 1:200; Acta2, mouse, Sigma, 1:800 ...
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bioRxiv - Plant Biology 2021Quote: ... 10%glycerol, 1 mM DTT, 1 mM EDTA, 0.5% Triton X-100 and 1% [v/v] protease inhibitor cocktail; Sigma-Aldrich). Insoluble debris was pelleted by centrifugation for 20 min with 4000 rpm at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Non-infected and Lm-infected HUVEC were incubated for 1 h with primary antibody rabbit anti-STAB-1 (1:100, Millipore), diluted in 0.2% saponin (Merck ...
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bioRxiv - Genomics 2021Quote: ... The beads were washed two times with high-salt wash (50 mM Tris-HCl, pH 7.4, 1 M NaCl, 1 mM EDTA, 1% Igepal CA-630 (Sigma I8896), 0.1% SDS ...
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bioRxiv - Physiology 2021Quote: ... 0.5% Na-deoxycholate and 1% Triton X100) containing protease inhibitors and phosphatase inhibitor cocktail 1 and 2 (1:100, Sigma). Protein lysates were separated on a 4-15% TGX gel (Biorad ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse monoclonal anti-Na+/K+ ATPase (1:500; SantaCruz) and anti-acetylated tubulin (1:2,000; clone 6-11B-1, Sigma Aldrich). Goat anti-mouse or rabbit IgG conjugated with HRP were used for secondary antibodies (1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... and cultured with RPMI 1640 plus GlutaMAX + 1% (v/v) PS + 1% (v/v) B-27 + 1 μM thyroid hormone (T3) (Cat. No. T6397, Sigma) + 10 μM ALK5 inhibitor + 10 μM zinc sulfate (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... the retinas were dissected and rinsed several times with 1× PBS and incubated for 15 minutes at 1:1000 dilution of PBS with 1 mg/ml Nile Red (Sigma).
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bioRxiv - Neuroscience 2021Quote: ... Cells were blocked with 5 % BSA/PBS for 1 h before overnight incubation with primary antibodies in 1 % BSA/PBS (RAB5 21435 1:200 Cell Signalling and anti-Integrin-β1 MAB1997 1:1000, Millipore) at 4°C prior to washing and incubation with secondary antibodies (anti-rabbit AlexaFluor-546 [A11-35] and anti-mouse AlexaFluor-633 [A21052] Thermofisher ...
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bioRxiv - Developmental Biology 2020Quote: ... buffered to pH 8.3 with 1 M bicine) supplemented with 1 g/l of and Antibiotic antimycotic solution (1:100, Sigma, A5955). To dissociate into single cells ...
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bioRxiv - Genetics 2021Quote: ... Samples were then digested for 15min at room temperature using an enzymatic solution (1% driselase, 0.5% cellulase, 1% pectolyase, 1% BSA, all from Sigma-Aldrich), and washed 3 times in PBS ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of biotinylated secondary antibody diluted in PBS-BN (anti-human IgG, B1140, 1/1250; anti-human IgM, B1265, 1/1000; or anti-human IgA, SAB3701227, 1/500; Sigma) were added to all wells and incubated for 45 min at 600 rpm at RT and protected from light ...
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bioRxiv - Immunology 2021Quote: ... Positive transformants were selected on LB medium (1% tryptone, 0.5% yeast extract, 1% NaCl; Oxoid) supplemented with ampicillin (100 µg mL−1, Sigma Aldrich) for 24h at 37°C ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... Samples were dehydrated with methanol series (50%, 75%, 95%, 100%, 100%) and overnight incubated in 1:1 methanol : BABB (BenzylAlcohol-BenzylBenzoate 1:2, both Sigma)) ...
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bioRxiv - Molecular Biology 2021Quote: ... infected in BGM containing Adeno-GFP (1×1010) or Adeno-ADH5 (1×1012) and differentiated in BGM containing 0.25nM 3-Isobutyl-1-methylxanthine (IBMX, Sigma, I5879), 1uM Rosiglitazone (Sigma ...
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bioRxiv - Immunology 2020Quote: ... 50 μL of each culture supernatant were mixed with 50 μL of Griess reagent [1:1 (v/v) 0,1% N-(1-naphthyl) ethylenediamine dihydrochloride (NEED, Sigma-Aldrich) in water ...
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bioRxiv - Microbiology 2022Quote: ... colistin [850 U ml−1]; gentamicin [0.035 mg ml−1]; and vancomycin [0.045 mg ml−1] [all Sigma Aldrich, UK]) was administered ad libitum in the drinking water as previously described (Jukes et al. ...
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bioRxiv - Neuroscience 2022Quote: ... and rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1) (1:350; Wako) to evaluate the inflammatory infiltrate and rabbit anti-myelin basic protein (MBP) (1:100; Millipore) to examine demyelination ...
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bioRxiv - Cell Biology 2023Quote: ... centrifuged and the cell pellet was lysed using a Lysis Buffer (25 mM Tris pH 7.6, 150 mM NaCl, 1% NP-40, 1% Na-deoxycholate, and 0/1% SDS in water + protease inhibitor cocktail [Sigma] + phosphatase inhibitor cocktail [Sigma-Aldrich] + okadaic acid + sodium fluoride) ...
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bioRxiv - Microbiology 2023Quote: ... Approximately 1 mL nitrate stock solution (10 g N L-1) containing ∼1% 15N-labelled sodium nitrate (98 atom % 15N, Sigma) was added to achieve a concentration of ∼20 mg N L-1 ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS with 0.1% Triton X-100 for 1 h at room temperature and then immunolabeled with anti-FLAG (1:1000, F1804, Sigma) overnight at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Dried samples were first resuspended in a 1:1 v/v mixture of a 1% solution of N,N-diisopropylethylamine (Sigma) and a 1% solution of pentaflurobenzylbromine (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... monitored at 340-380 nm (ε = 4.81 mM-1 cm-1) and its inhibitor sensitivity checked by addition of 1 μM piericidin A (Sigma). Succinate:O2 oxidoreduction was initiated by addition of 5 mM succinate ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (rat anti-BrdU, 1:100, Abcam; mouse anti-BrdU, 1:100,BD Biosciences; mouse anti-Nestin, 1:300, Millipore; rabbit anti-GFAP ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mM NaPO4 buffer, 5 mM EDTA, 50 % deionised formamide, 1 mg ml-1 tRNA, 1 x Denhardt’s solution [Sigma-Aldrich, D2532] ...
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bioRxiv - Immunology 2024Quote: Granulomas were identified on paraffin sections by light microscopy and serial sections were used for immunofluorescence staining for Arginase-1-AF568 (1:800) and F4/80-AF647 (BM8, 1:300, Ebiosciences) and DAPI (Sigma). Stained tissue sections were imaged with an inverted point scanning confocal microscope (Zeiss LSM 710 ...
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bioRxiv - Plant Biology 2023Quote: ... sections of nodules were incubated with calcofluor white (1 g L-1) in Evans blue as a background stain (0.5 g L-1) (Sigma-Aldrich) according to Wood et al ...
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bioRxiv - Zoology 2024Quote: ... with dip-rinsing before and after treatments: (1) 1 µm filtration of microalgae cell suspension (Millipore 1 µM filter SLFAO5010), to remove diatom cells but retain microbial cells ...
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bioRxiv - Cancer Biology 2023Quote: ... apoptosis was measured by incubating 1 million cells for 1 hour in 1 microliter/ml FITC-VAD-FMK (EMD-Millipore) to detect apoptotic cells by flow cytometry ...
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bioRxiv - Plant Biology 2024Quote: ... Transformants were selected with appropriate antibiotic(s) (hygromycin B, 20 µg mL-1; paromomycin, 10 µg mL-1; spectinomycin, 100 µg mL-1 Sigma-Millipore) for a week before screening them for fluorescence in 96 well plates ...
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bioRxiv - Molecular Biology 2022Quote: ... mouse anti-γ-H2AX JBW301 (1:1000 for IB, 1:2500 for IF, 1:5000 for FACS; Millipore, 05-636), mouse anti-γ-H2AX-Alexa Fluor 647 (1:1000 for FACS ...
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bioRxiv - Biochemistry 2022Quote: ... 50 μg ml-1 spectinomycin (Sp) or/and 35 μg ml-1 chloramphenicol (Cm) or 100 μg·ml-1 ampicillin (Sigma, Merk).
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bioRxiv - Bioengineering 2022Quote: ... was assembled around the region containing photopatterned ssDNA features and wells were incubated in 10-20 μg ml-1 bovine serum fibronectin (1 mg ml-1, F1141, Sigma) diluted in sterile 50 mM HEPES (pH 8.5 ...
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bioRxiv - Bioengineering 2022Quote: ... ionised calcium-binding adapter molecule 1 (IBA1; 1:500, 019-19741, Wako) and neural nuclei (NeuN; 1:500, ABN90P, Sigma). The day after ...
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bioRxiv - Microbiology 2022Quote: ... and incubated with energy buffer (1 mM ATP, 1 mM UTP, 1 mM MgCl2, 10 mM creatine phosphate (Sigma 2380), 1 mg/mL creatine phosphokinase (Sigma 2384) ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000; NeuN, guinea pig, Millipore, 1:2000; Sox9, rabbit, Millipore, 1:1000 ...
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bioRxiv - Microbiology 2022Quote: HeLa cells were seeded in 24-well plates (1 x 105 cells x ml-1) and incubated for 24 h before 8 µg x ml-1 Polybrene (Sigma) was added to the cells ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were incubated overnight in primary antibodies targeting LC3 (1:2000) and p62 (1:2000) and aSyn (1:500) diluted in blocking solution with 0.1% Tween-20 (Sigma-Aldrich). The next day ...
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bioRxiv - Cell Biology 2022Quote: ... Cell layers were incubated with primary antibodies against fibrillin-1 (described previously, 1:1000) and fibronectin (clone FN-15, 1:1000, Millipore-Sigma) diluted in blocking buffer for 2 h at RT [31] ...
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bioRxiv - Cell Biology 2023Quote: ... His6-T7-HSV-tagged full length Plasmid pRSF-Duet-SNAP25-StxΔTM co-expressing untagged SNAP25 and syntaxin 1 (1-265) was obtained by successively inserting syntaxin (1-265) sequence into NdeI / XhoI sites of pRSF-Duet-1 (Novagen) and SNAP25 sequence into the NcoI / EcoRI sites of the resulting plasmid ...
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bioRxiv - Cancer Biology 2023Quote: Cells were lysed in Sodium Dodecyl Sulfate (SDS) buffer (SDS 1%; pH 7,4; Tris 10 mM Na3VO4 1 mM) supplemented with 1:500 diluted benzonase (Sigma-Aldrich). Protein concentration was determined using the Bicinchoninic acid assay (BCA ...
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bioRxiv - Plant Biology 2024Quote: ... 50 μL of extraction buffer (100 mM Tris pH 7.5, 150 mM NaCl, 10 % [v/v] glycerol, 10 mM EDTA, 1 mM DTT, 1 mM PMSF, 1 mM Sigma protease inhibitor and 1 % [v/v] IPEGAL CA-630 ...
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bioRxiv - Neuroscience 2024Quote: ... in PBS for 1 h at RT to block nonspecific staining and incubated with the appropriate primary antibody (anti-MAP2 [1:10,000; ab5392, Abcam], anti–PSD-95 [1:200; 7E3-1B8, Thermo Scientific], and anti-vGluT1 [1:5000; AB5905, Millipore]) diluted in 3% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... Primary antibodies (anti-Flag, 1:1000, Sigma, F3165; anti-USP30, 1:500, Sigma, HPA016952; anti-Vinculin, 1:10000, Sigma, V9131) were allowed to bind overnight ...
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bioRxiv - Immunology 2024Quote: ... Clearing was initiated by incubating samples in a 1:1 mixture of methanol and BABB (benzyl alcohol and benzyl benzoate at a 1:2 ratio, Sigma ref ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 g of FFA was diluted in 10 mL ethanol/diethyl ether (1:1; Sigma-Aldrich, Saint-Louis, MO, USA) and then LiOH (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-FLAG-tag (CST, rabbit, 1:1000), anti-total-ERK1/2 (CST, rabbit, 1:1000) diluted in PBS containing 1% BSA (Sigma); and secondary antibody anti-rabbit (CST ...
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bioRxiv - Neuroscience 2024Quote: ... Type and dilution of secondary antibodies was the same for vertical sections and whole-mounts (Alexa Fluor 488, 568, and 647, 1:500, Invitrogen; Alexa Fluor 405, 1:500, Abcam; CF568, 1:500, Sigma).
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bioRxiv - Molecular Biology 2024Quote: ... hnRNP K (RN019P, 1:1000, MBL), hnRNP E1 (RN024P, 1:1000, MBL) and β-actin (#A20066, 1:5000; Sigma-Aldrich). Membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (1:5000 ...