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Citations for Millipore Sigma :
2301 - 2350 of 10000+ citations for Tick Borne Encephalitis Virus Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... mAb anti-α-tubulin (T6199; IF: 1/4000) and pAb anti-Flag-tag (F7425; IF: 1/500, WB: 1/1000) were from Sigma-Aldrich, and a pAb anti-GFP (632592 ...
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bioRxiv - Cell Biology 2021Quote: ... the supernatants obtained were incubated with gentle rotation for 1 h at 4°C with anti-FLAG tag-antibody-conjugated magnetic beads (Sigma-Aldrich). The beads were then washed three times with lysis buffer #1 and incubated with gentle rotation for 30 min at 4°C with 50 μL of an elution buffer (50 mM HEPES-KOH ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were eluted using 250 µg/mL Flag peptide into wash buffer for 1 h at 4°C and subjected to immunoblotting analysis using antibodies for Flag tag (SIGMA, SLCD6338) and SARS-CoV-2 S (Sino Biological ...
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bioRxiv - Biochemistry 2021Quote: ... were constructed using standard genetic engineering techniques with a His6 tag at the N-terminus using the pET28a vector (Novagen, Merck). The recombinant CAHS1 protein ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
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bioRxiv - Immunology 2022Quote: ... followed by blocking for 1 h with 5% nonfat milk in Tris-buffered saline-Tween 20 buffer and probing with antibodies against Strep Tag (SAB2702215, Sigma-Aldrich), γ-catenin (sc-514115 ...
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Biochemistry 2020Quote: ... N-terminally His-tagged MpPA14 was incubated with the array and binding of the lectin to the polysaccharides was detected by an anti-His tag secondary antibody conjugated to alkaline phosphatase (Sigma-Aldrich). Microarray probing and quantification were performed as previously described(41).
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bioRxiv - Microbiology 2021Quote: ... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663 ...
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bioRxiv - Molecular Biology 2021Quote: ... un-spliced precursor and mutant SufB proteins carrying an N-terminal 6X-His-tag were over-expressed in BL21 (DE3) E.coli cells via IPTG (500µM) (Sigma 367-93-1) induction at 37°C for 4hrs ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was nutated with 200 μL of Ni-NTA resin (Roche cOmplete™ His-Tag purification resin, Millipore Sigma #5893682001) at 4°C for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... and then monoclonal anti-6x(HIS)-tag antibody was probed followed by detection with anti-mouse IgG conjugated with alkaline phosphatase (A3562: 1ml Sigma, USA) was used for developing the blots.
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated by SDS-PAGE on a 4-20% gradient gel and then immunoblotted as described above using antibodies directed against the GST tag (Sigma-Aldrich) to detect CBUA0027 ...
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bioRxiv - Bioengineering 2022Quote: ... samples were washed 3× five minutes in BSA-T and incubated with an antibody against the FLAG-tag (#F1804 Sigma-Aldrich, now Merck ...
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bioRxiv - Cell Biology 2022Quote: ... oligos carrying the ALFA tag sequence codon optimized for yeast and flanked by SalI and BglII sites were synthesized by Sigma-Aldrich. These oligos were annealed in annealing buffer (10 mM Tris pH 7.5-8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Cleavage of the tag was performed in 2-3 ml with 20 μl 3C protease (1 mg/ml, Sigma-Aldrich, SAE0045) added and incubated over-night at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated by SDS-PAGE on a 4-20% gradient gel and then immunoblotted as described above using antibodies directed against the GST tag (EMD Millipore) to detect CBUA0027 ...
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bioRxiv - Microbiology 2023Quote: ... gBlock contained vector-specific sequence) methods into pET28a (cytochromes, NdeI/XhoI sites, in frame with N-terminal His6 tag, Novagen 69864) or pGEX (HCCS/HCC1S ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked at room temperature for 1 hour in 1% casein/PBS and probed overnight at 4°C with a 1:1000 dilution of Roche monoclonal 3F10 rat anti-HA-tag antibody (Sigma 11867423001) and 1:1000 dilution of rabbit anti-P ...
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bioRxiv - Immunology 2023Quote: ... supplemented with Phos-tag Acrylamide (Wako Chemicals, AAL-107) to a final concentration of 30μM and manganese chloride(II) (Sigma-Aldrich, #63535) to 60μM ...
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bioRxiv - Bioengineering 2023Quote: A 10 mg/mL suspension of TAG drops was prepared by dissolving 1 mg of 1,2-dioleoyl-3-palmitoyl-rac-glycerol (Sigma-Aldrich Inc.) in 100 μL of a chloroform–methanol solution (2:1) ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells stably expressing CCR5 were labeled with 2ug/ml rabbit anti-Flag tag antibody (F7425, Sigma, St. Louis, MO, USA) in FACS buffer (PBS with 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Microbiology 2023Quote: ... The final dilution of the primary antibody was 1:2,000 for anti-HA tag (Covance) and 1:3000 for anti-β-actin (Millipore-Sigma).and K-Rta (65).
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bioRxiv - Biochemistry 2023Quote: ... were cloned into pHAT4 (Peranen et al., 1996) for expression as a TEV-cleavable His6-tag fusion in BL21 (DE3) cells (Novagen®) in 2xYT media ...
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bioRxiv - Biochemistry 2023Quote: ... and BRCA2 Ex14-AAA were cloned into pMAT11 for expression as TEV-cleavable His6-MBP-tag fusions in BL21 (DE3) cells (Novagen®) in 2xYT media ...
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bioRxiv - Biochemistry 2024Quote: ... numbering includes the signal peptide) with C-terminal Flag and 6x-Histidine tags and subsequently cloned into a p32A vector (Millipore Sigma). The TrxA-3C-ligand-Flag-6xHis construct was transformed into Origami E ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells were seeded at a density of 2.5×106 cells/well in 12-well plates and spin-fected (2000 rpm for 2 hours at 37°C) with increasing concentrations of virus in the presence of 8µg/mL polybrene (Millipore TR-003-G lot#3287963). The next day ...
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bioRxiv - Cell Biology 2022Quote: ... Near-confluent cells were transduced by aspirating the medium and adding 1ml of the respective virus diluted in 1ml of EGM2 with 4mg/ml polybrene (Sigma, Prod. No. H9268-5G). Cells were then left to incubate for 6h at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... Wild-type KP4 cells were transduced with concentrated virus and GFP-expressing cells were selected using puromycin selection (1 μg/mL, Sigma-Aldrich, St Louis, USA). GFP-expressing PDOs were created using the same virus as KP4 but with a slightly different transduction protocol ...
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bioRxiv - Microbiology 2023Quote: ... Cells were further incubated in medium containing 300 nM tamoxifen for 48 h and then incubated with two-fold dilutions of AdV-C5-EGFP stock virus in DMEM medium (Sigma-Aldrich, cat. no. D6429) supplemented with 7.5% fetal calf serum (Gibco/ Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Boost vaccination was performed with the homologous virus inactivated at 4°C for 3 days with 0.1% beta propiolactone (BPL) (Sigma-Aldrich Corporation, St. Louis, MO) as previously described (66) ...
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bioRxiv - Cancer Biology 2023Quote: ... RMG-1 ARID1A-WT and ARID1A-KO cells were seeded at a density of 2.5×106 cells/well in 12-well plates and spin-fected (2000 rpm for 2 hours at 37°C) with increasing concentrations of virus in the presence of 8µg/mL polybrene (Millipore TR-003-G lot#3287963). The next day ...
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bioRxiv - Cell Biology 2023Quote: ... on day 3 of activation (Fig. S1A) 250 µl of concentrated virus was preincubated with 8 µg/mL polybrene (EMD Millipore, TR-1003-G) for 30 min on ice ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: MEF cells were plated in 10 cm dishes to give a 70% confluency the following day for viral transduction by the addition of 5 ml of virus with 5 ml of fresh medium and 10 μg/ml Polybrene (Sigma-Aldrich, TR-1003-G). After 24h incubation ...
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bioRxiv - Microbiology 2020Quote: ... protein complexes were precipitated using Protein A agarose beads (Sigma, St. Louis, MO, USA). The precipitated material was analyzed by western blot ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: The protein content was analysed with the Bicinchonic Acid Protein Assay Kit (Sigma-Aldrich) according to the manufacturer’s protocol with minor adjustments ...
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bioRxiv - Cell Biology 2021Quote: The Protein A slurry was prepared by washing 1.5 g Protein A Sepharose (Sigma) 4 times in 1X Tris Buffered Saline (1X TBS ...
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bioRxiv - Neuroscience 2021Quote: ... Bradford protein assay was used to quantify total protein levels (Bradford, Sigma-Aldrich, USA) and to normalize BDNF data.
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bioRxiv - Physiology 2022Quote: ... Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150, Merck Millipore) following the manufacturer’s instructions ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Bound proteins were captured with Protein G agarose for 4 h (Sigma-Aldrich, #17061801), spun at low speed ...
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bioRxiv - Cell Biology 2023Quote: ... and total (i.e. nonphosphorylated) protein levels were normalized to tubulin protein levels (Sigma-Aldrich, #T6557 ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 μg of protein were added to washed agarose protein A beads (Merck Millipore) and incubated on a rotating wheel for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: Total carbonylated proteins were quantified using OxyBlot Protein Oxidation Detection Kit (Millipore, Billerica, MA) following manufacturer’s instructions using 15 μg of total protein detected with rabbit anti-DNP antibodies (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein precipitation was carried out using ProteoExtract Protein Precipitation Kit (EMD Millipore, 539180-1KIT) according to the manufacturer’s protocol and submitted for MS analysis to the Taplin Biological Mass Spectrometry Facility.
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bioRxiv - Developmental Biology 2021Quote: ... Cell pellet was resuspended in 11.7 mL of 10% FCS and then fixed by adding 325 μL of 37% Formaldehyde (Sigma-Aldrich) (final 1% FA ...
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bioRxiv - Genomics 2020Quote: ... The bottom oil layer was removed and replaced with FC-40 fluorinated oil (Sigma-Aldrich, catalog no. 51142-49-5) with 5% (w/w ...
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bioRxiv - Genomics 2020Quote: ... The bottom oil was then removed and replaced with FC-40 fluorinated oil (Sigma-Aldrich, catalog no. 51142-49-5) with 5% (w/w ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse recombinant EphrinB1-Fc chimera (cat # E0653-200UG) and bovine serum albumin (cat #05470-1G) with purity >98 % were from Sigma. Alexa Fuor-594 BSA (cat #A13101) ...
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bioRxiv - Developmental Biology 2019Quote: ... in 400ul of a solution containing 1x PBS complemented with 10% FCS and 5ul Collagenase 10mg/ml (sterile filetered, C1764 Sigma). Fixation was done in a final concentration of 1% FA (w/o methanol ...
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bioRxiv - Developmental Biology 2019Quote: ... in 250ul of a solution containing 1x PBS complemented with 10% FCS and 5ul Collagenase 10mg/ml (sterile filtered, C1764 Sigma). Fixation was done in a final concentration of 2% PFA for 10 minutes at room temperature ...