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Citations for Millipore Sigma :
2251 - 2300 of 10000+ citations for Tick Borne Encephalitis Virus Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cells were incubated in medium containing 2% FCS with or without the PAR2 negative allosteric modulator (AZ3451, Sigma Aldrich) or a NF-κβ inhibitor (Bay11-0782 ...
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bioRxiv - Microbiology 2020Quote: ... FMC Biopolymer (90) in DMEM (containing 2% FCS and antibiotics) and serial dilutions of 5-FU (F6627, Sigma-Aldrich) or Ribavirin (R9644 ...
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bioRxiv - Microbiology 2020Quote: ... The mice were boosted subcutaneously (s.c.) at day 8 with 50 μg of RBD-Fc plus Freund’s Adjuvant Complete (Sigma, USA) and at day 13 with 50 μg of RBD-Fc plus Titermax adjuvant (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... Digestion of the colon tissue was performed in RPMI/10% FCS containing 0.4mg/ml type VIII collagenase (Sigma Aldrich) and 40μg/ml DNase I (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Inocula were removed and replaced with DMEM containing 1% FCS and a semi-solid overlay consisting of 1.5% carboxymethyl cellulose (SIGMA). Cells were incubated for 72h ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed in RPMI supplemented with 10% FCS and incubated with 10 μM of MG132 (Sigma Aldrich) for 3h ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were frozen at 1 x 107 cells per 1 ml of freezing medium (FCS, 10 % DMSO (Sigma-Aldrich)) ...
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bioRxiv - Microbiology 2023Quote: ... Parasites containing restored S14 loci were selected by negative selection using a 5-fluorocytosine (5-FC) drug (Sigma, USA) as previously described (Srivastava & Mishra ...
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bioRxiv - Genetics 2023Quote: ... in supplemented RPMI1640 medium (10% heat-inactivated FCS, 2mM L-glutamine, 50IU/ml penicillin, 50μg/ml streptomycin; Sigma-Aldrich), 1×106 MLN cells/well were stimulated by 50ng/ml PMA and 1μM ionomycin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... taken up in 40ml of FCS-free DMEM supplemented with 480 μl of 10 mg/ml Trypsin (Sigma, T9935) and 800 μl of 50 mg/ml Collagenase II (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Eurobio Scientific, CVFSVF00-01) and containing 1% penicillin-streptomycin (Sigma-Aldrich, P4333). HEK-293T were grown in Hepes-modified DMEM (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... and digested in 20 mL of DMEM added with 20% FCS and 100 U/ml of collagenase (Sigma-Aldrich) for 40 min at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... at a density of ∼2.5*106 cells/mL in 5% fetal calf serum (FCS) (Bodinco BDC, 16941) DMEM (Dulbecco’s Modified Eagle’s Serum (Sigma, 05796), 1% Pen-Strep (Lonza ...
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bioRxiv - Genomics 2024Quote: ... at a density of ∼2.5*106 cells/mL in 5% fetal calf serum (FCS) (Bodinco BDC, 16941) DMEM (Dulbecco’s Modified Eagle’s Serum (Sigma, 05796), 1% Pen-Strep (Lonza ...
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bioRxiv - Microbiology 2024Quote: ... precoated with fibronectin-collagen IV (10 μg.ml-1) and grown in DMEM containing 10% Fetal Calf Serum (FCS) and 1 μM dexamethasone (Sigma) for 14 days to allow proper differentiation ...
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bioRxiv - Neuroscience 2024Quote: ... Forty-eight hours after transfection the medium with soluble RGMA-Fc or APP was concentrated through a 50,000 kDa column (YM-50, Millipore). The concentrated proteins were supplemented with Dulbecco’s modified Eagle’s medium 1 g/L glucose (DMEM ...
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bioRxiv - Microbiology 2024Quote: ... The secondary antibody used was goat anti-mouse IgG (Fc specific) conjugated with alkaline phosphatase from Sigma (CAT#A1418) and used at 1:3000 dilution.
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bioRxiv - Molecular Biology 2020Quote: ... protein was isolated from cells using 300μl CytoBuster™ Protein Extraction Reagent (Novagen) per well according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extracts were performed with the Plant Total Protein Extraction Kit (Sigma, USA). Protein concentrations were measured using the BCA method ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were sonicated and protein concentration was determined using BCA protein assay (Sigma). 30 μg of total proteins were loaded on 12% SDS–PAGE gel and separated by electrophoresis ...
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bioRxiv - Evolutionary Biology 2019Quote: ... a marker of protein oxidation (protein carbonyls; OxyBlot; s7150; EMD Millipore, Billerica, MA), and a marker of mitochondrial biogenesis (PGC-1α ...
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bioRxiv - Plant Biology 2020Quote: ... were subjected to protein extraction using Plant Total Protein Extraction Kit (Sigma-Aldrich). The protein concentration was measured using 2-D Quant Kit (GE Healthcare ...
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bioRxiv - Genomics 2019Quote: Duolink PLA kit was used to detect protein-protein interactions (Sigma Aldrich DUO92101). PLA was performed according to manufacturer’s protocol plus additional negative controls ...
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bioRxiv - Genetics 2020Quote: ... Protein concentrations were analyzed with a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich), using bovine serum albumin as a standard ...
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bioRxiv - Immunology 2020Quote: Total proteins isolated from BMDCs were lysed in CytoBuster protein extraction reagent (Novagen). Protein quantification was performed using NanoDrop One Spectrophotometer ...
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bioRxiv - Cell Biology 2022Quote: ... the other stained for proteins with SYPRO Red Protein Gel Stain (Sigma Aldrich). Both dyes were used according to the manufacturers’ protocols.
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bioRxiv - Microbiology 2022Quote: ... total cell protein was measured using the bicinchoninic acid protein assay (Sigma-Aldrich) against bovine serum albumin standards ...
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bioRxiv - Cancer Biology 2023Quote: Oxidized proteins were detected using the OxyBlot Protein Oxidation Detection Kit (Sigma, S7150) and following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was quantified using bicinchoninic acid (BCA) Protein assay (Sigma-Aldrich). Then 60 μg of the total protein of each genotype was separated on 8% SDS-PAGE and transferred to the PVDF membrane (Amersham ...
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bioRxiv - Microbiology 2023Quote: ... 1000 µg of protein extracts were cleared with agarose protein A beads (Millipore) at 4 ℃ for 1.5 h on a rocking platform ...
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bioRxiv - Microbiology 2023Quote: ... or with slurry protein G beads (Sigma-Aldrich Fast Flow Protein G sepharose). For GFP-p65 and GFP-COMMD2 the samples were immunoprecipitated as per manufacturer’s instructions with the elution was recovered in either 5x with Laemmli buffer 74 and boiled at 98°C (dry bath ...
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bioRxiv - Plant Biology 2023Quote: ... the protein extract was incubated with 30 μL Protein A Agarose beads (Millipore), 10 μL anti-SIZ1 antibody with rotation at 4°C for 4 h.
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bioRxiv - Microbiology 2023Quote: ... The protein was purified using a Protein A agarose gravity column (Millipore Sigma) followed by SEC using a S200 column.
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bioRxiv - Biochemistry 2023Quote: ... Protein concentrations were determined with the BCA protein assay kit from Sigma-Aldrich.
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bioRxiv - Molecular Biology 2024Quote: The protein mixtures were filtered using a low protein binding filter (Millipore Sigma) and purified on a SourceQ15 anion exchange column (Cytiva ...
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bioRxiv - Genomics 2024Quote: ... Protein transfer and total protein content was assessed by Ponceau Staining (Sigma Aldrich). Ponceau Stain was washed out with 1x TBST ...
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bioRxiv - Genetics 2024Quote: ... protein concentration was measured by use of a BCA Protein Assay (SIGMA Aldrich) and samples stored at -80°C.
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bioRxiv - Molecular Biology 2021Quote: Protein-G (Protein G Sepharose® 4 Fast, cat# GE17-0618-01) or Protein-A (ProteinA-Sepharose® 4, cat#P9424 Millipore) beads were washed twice with 1x PBS and twice with IP100 buffer (25 mM Tris-HCl 7.9 ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; human α-syn: 804-258-1001, Enzo Life Science; beta-actin: A4700, Sigma). After incubation with the first antibody ...
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bioRxiv - Immunology 2022Quote: Concentrations of human serum albumin in human plasma and bronchoalveloar lavage fluid were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0603) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies for immunostaining included rabbit anti-human/mouse PKD-1 (SAB4502371) and mouse anti-human/mouse α-smooth muscle actin (α-SMA, A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Cancer Biology 2022Quote: The peptides mUNO (sequence: CSPGAK-COOH) and FAM-mUNO (FAM-Ahx-CSPGAK-COOH) were purchased from TAG Copenhagen and doxorubicin (DOX) from Sigma-Aldrich. St-PGA was kindly provided by Polypeptide Therapeutic Solution S.L ...
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bioRxiv - Biophysics 2019Quote: Human Gai1 subunit with an amino-terminal 8x histidine tag followed by a rhinovirus 3C protease side were expressed in Rosetta 2 (DE3) cells (EMD Millipore) using pET21a ...
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bioRxiv - Synthetic Biology 2019Quote: ... the 6x-His Tag Polyclonal Antibody or HA Tag Polyclonal Antibody was the primary antibody and the anti-rabbit IgG-gold (10 nm) antibody (Sigma-Aldrich) was the secondary ...
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bioRxiv - Cell Biology 2021Quote: ... The gDNA extractions and PCR verifying integration of the fluorescent tags into the genomic loci was performed using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). PCR amplicons were run on 1 or 2% agarose gels in TBE and gels were imaged with Bio-Rad Gel ChemiDoc XRS system and Quantity One software.
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bioRxiv - Biochemistry 2020Quote: ... Western blot analysis was used to verify the identity of PfAdoMetDC using monoclonal α-Strep-tag II antibodies (Novagen, Wisconsin, USA). Following Strep-Tactin Sepharose affinity column purification ...
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Cell Biology 2022Quote: ... It was inserted with N and C-terminal tags (His6 and StrepII, respectively) in the baculovirus transfer vector pIEX/Bac3 (Merck Novagen #717263).