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Citations for Millipore Sigma :
2301 - 2350 of 10000+ citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 1.47 mM KH2PO4 supplemented with 5 mM DTT and concentrated to ∼10-20 mg/mL using 3 kDa Millipore concentrator and stored at -80 °C.
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bioRxiv - Immunology 2019Quote: ... The T cells were placed above 500 μl conditioned media supplemented with 3-5 μg/ml ConA (Sigma Aldrich) in 24-well plates ...
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bioRxiv - Developmental Biology 2019Quote: ... short-hairpin RNA against PRKCI target sequence 5’-AGTACTGTTGGTTCGATTAAACTCGAGTTTAATCGAACCAACAGTACT-3’ was used to generate lentiviral particles (Sigma Mission-TRCN0000219727). Lentiviral particles were generated by transfecting HEK293T cells ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were washed 3 times for 5 min and incubated with anti-mouse-HRP antibody (0.2 μg/ml – Millipore) for 1 h in TBST ...
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bioRxiv - Plant Biology 2020Quote: ... The bottom of the tilted plates was filled with 13 ml of 0.5 M glucose solution for glucose supplementation experiments or 100 μM 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU, Sigma®) for sugar starvation experiments ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Cre reverse primer 5’-GGT TTC CCG CAG AAC CTG AA-3’ (both primers were purchased from Sigma-Aldrich).
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bioRxiv - Molecular Biology 2020Quote: ... Cell disruption was carried out by vortexing (3 cycles, 5 min each) at 4°C using 0.2 ml of glass beads (425-600 μm; Sigma). For the Rpb3 immunopurification ...
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bioRxiv - Cell Biology 2022Quote: ... the pellet was resuspended in 3 mL Nuclear Permeabilization Buffer (1X PBS, 5% Bovine Serum Albumin, 0.2% IGEPAL CA-630 (Sigma), 1 mM DTT ...
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bioRxiv - Cell Biology 2022Quote: ... was revealed with 5-bromo-4-chloro-3-indolyl β-D galactoside (X-gal) (Sigma-Aldrich, Carlsbad, CA, USA). Diploid cells were grown on SC medium supplemented with 20 mg/L adenine hemisulfate ...
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bioRxiv - Microbiology 2022Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, St. Louis, MI, USA) solution was applied to the cells and incubated for an additional 4h ...
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bioRxiv - Neuroscience 2022Quote: ... Passive dextran permeability was measured by culturing iBECs on Ø 0.4 μm pore polyester or polycarbonate Transwell inserts and cells were exposed to 0.5 mg/mL fluorescein isothiocyanate (FITC)-conjugated 3 – 5 kDa dextran (Sigma) for 24h ...
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bioRxiv - Plant Biology 2024Quote: ... They were then resuspended in 10 mM MES-KOH buffer (pH 5.7) with 10 mM MgCl2 and 100 mM acetosyringone (49-hydroxy-3′,5′-dimethoxyacetophenone; Sigma) to achieve a final OD600 of 0.5 ...
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bioRxiv - Microbiology 2023Quote: ... 30 μL of MTT [(3- [4,5-dimethyl-2-thiazolyl] -2,5-diphenyl-2H-tetrazolium bromide; 5 mg/mL; (Sigma-Aldrich)] was added in each well and the plate incubated for 3 hours at 25°C ...
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bioRxiv - Microbiology 2024Quote: ... Coloring reactions were performed with nitro blue tetrazolium/5-Bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) solution (Sigma), and then sections were washed in PBS ...
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bioRxiv - Immunology 2024Quote: ... PCR was performed to add the 3XFlag tag to the N terminus of ZBP1 cDNA at the 5’ Not1 site and 3’ Sal1 site to facilitate cloning into expressing vector p3XFlag-CMV-7.1 (Sigma). The resulting plasmid containing the 3XFlag-ZBP1 was further subcloned in the Bst1 and BamHI sites of the vector pLVX-EF1α-AcGFP1-C1 (Takara ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking of endogenous peroxidases (3% H2O2) and unspecific antibody binding (2% BSA+5%serum) PLAC8 antibody (HPA040465, Sigma) or SARS-CoV-2 Spike Glycoprotein S1 antibody (GTX632604 ...
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bioRxiv - Neuroscience 2022Quote: ... The dopamine- synthesis inhibitor 3-Iodo-L-tyrosine (3IY; CAS: 70-78-0, Sigma Aldrich; concentration: 5 mg/ml) was added to samples of 2 ml yeast solution ...
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bioRxiv - Microbiology 2022Quote: ... cultures were pelleted by centrifugation at 1,600 rpm for 3 min and resuspended in a 10× volume of 5% sorbitol (Sigma), followed by incubation at 37°C for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... by dispensing individual cells in 5 nL drops directly into 3 µL ice-cold single cell lysis buffer (scLB, 0.134% Triton X-100 [Sigma] ...
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bioRxiv - Neuroscience 2023Quote: ... 3-5 organoids per condition were dissociated by incubation with a solution of 0,5 mg/ml Collagenase/Dispase (Sigma) + 0,22 mg/ml EDTA (Euroclone ...
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bioRxiv - Microbiology 2023Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, St. Louis, MI, USA) solution was added to the cells and incubated for an additional 4 h ...
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bioRxiv - Cell Biology 2023Quote: The cell proliferation assay was determined by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay (Sigma). All cancer cell lines were seeded in 24-well plates (2×104 cells/well) ...
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bioRxiv - Developmental Biology 2023Quote: ... Fixed embryos were washed 3 times (5 minutes each) in wash buffer DPBS Tween containing 2% BSA (Sigma, A3311), and permeabilised at room temperature for 20 minutes in permeabilisation buffer 0.5% Triton-X in DPBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were again washed 3 × 5 min with PBS + 0.1% Tween 20 and incubated with Classico/Crescendo Western HRP substrate (Millipore-Sigma) for 2 min ...
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bioRxiv - Genetics 2023Quote: ... or a shRNA specific for human SOX11 (100% identity to the equine SOX11 sequence) (5’-CTGGTGGATAAGGATTTGGAT-3’ clone NM_003108.3-1235s1c1; Sigma-Aldrich) were created by transfection into HEK293T packaging cells ...
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bioRxiv - Cancer Biology 2023Quote: Culture medium was replaced with 100 µL medium containing 5 mg/mL filtered 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (#M5655, Sigma-Aldrich) and incubated for 2 h at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... media was aspirated from cells and replaced with 5 mg/mL 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT, #M5655, Sigma Aldrich) solution in base media ...
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bioRxiv - Physiology 2024Quote: ... The PDMS scaffold surface was activated oxygen plasma and then treated with a 5% (3-aminopropyl) triethoxysilane (Sigma-Aldrich) solution in methanol for 45 min ...
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Angiogenesis in the mature mouse cortex is governed in a region specific and Notch1 dependent mannerbioRxiv - Neuroscience 2024Quote: ... mice were intravenously injected with FITC-dextran (100µL of 3-5% w/v in 0.9% saline; 70kDa, Sigma #46945) or Texas Red dextran (100µL of 3% w/v in 0.9% saline ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with PBS (3 times x 5 minutes) and incubated with secondary antibodies and Hoescht 33342 (Sigma-Aldrich, #14533) diluted in 1x Antibody diluent/Block solution for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The small interfering siRNA (siRNA) oligonucleotide targeting human FCHSD2 (5’-GCAUACUCCUGAGACCUCA[dT][dT]-3’) was obtained from Sigma Aldrich. FCHSD2 siRNA knockdown in all HeLa cell lines was performed for 48 h using the DharmaFECT transfection reagent (Dharmacon ...
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bioRxiv - Plant Biology 2020Quote: ... supplemented with 10 g L−1 sucrose and 3 g L−1 phytagel (Sigma, Steinheim, Germany) or 8 g L−1 agar (Winkler ...
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bioRxiv - Neuroscience 2022Quote: ... The cleaned dishes were incubated with 1 mL of 1% (3-aminopropyl) triethoxysilane (APTES; Sigma Aldrich) in ethanol for 1hour at room temperature ...
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bioRxiv - Systems Biology 2022Quote: ... Cultures were treated with the indicated amount of 1-(1,1-dimethylethyl)-3-(1-naphthalenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (1-NM-PP1; Millipore Sigma), taken from a 10,000x stock in DMSO ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated on ice for 3 min and 2 mL wash buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.1 % Tween-20, 1 % BSA, 1 mM DTT, 1 U/µL Sigma Protector RNase inhibitor in nuclease free water ...
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bioRxiv - Physiology 2022Quote: ... Membranes were blocked for 1 hour at room temperature in 5% milk or 5% BSA (Millipore Sigma) in 1X Tris-buffered saline + 0.1% Tween 20 (TBST) ...
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bioRxiv - Microbiology 2021Quote: ... were plated on synthetic complete (SC) plates containing 1□mg/ml 5-FOA (5-fluorotic acid; Sigma) and on non-selective SC plates/ ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% Glycerol) supplemented with 1 mM Phenylmethylsulfonyl fluoride (Carl Roth) and 5 mM β-Mercaptoethanol (Sigma Aldrich). The resuspended cells were disrupted by sonication with a Branson Digital Sonifier (Model 102C ...
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bioRxiv - Cancer Biology 2023Quote: ... The 5 µl samples were injected onto a ZIC-pHILIC column (150 mm × 2.1 mm; i.d. 5 µm) with a guard column (20 mm × 2.1 mm; i.d. 5 µm) (Millipore) for LC separation ...
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bioRxiv - Bioengineering 2021Quote: ... Developer was applied with the SUEX master face-down in Propylene glycol methyl ether acetate (PGMEA, Sigma) until fully developed ...
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bioRxiv - Biochemistry 2020Quote: ... formic acid (FA) and tert-Butyl methyl ether (MTBE) were purchased from Sigma Aldrich (St. Louis, MO). Various lipid standards were purchased from Avanti Polar Lipids ...
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bioRxiv - Cell Biology 2021Quote: ... by positioning the embryos on agarose dishes containing 1.2-mm lanes with 4% methyl cellulose (Sigma-Aldrich) and filled with embryo medium (MMR 0.3x) ...
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bioRxiv - Plant Biology 2020Quote: ... and 1mM phenyl methyl sulfonyl fluoride [PMSF]) together with plant protease inhibitor cocktail (Sigma-Aldrich, http://www.sigmaaldrich.com/). Protein concentration was measured using nanodrop ...
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bioRxiv - Microbiology 2021Quote: ... Polymers of ethyl acrylate and methyl acrylate were synthesized from their monomers (99% pure, Sigma-Aldrich, Germany) using radical polymerization ...
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bioRxiv - Neuroscience 2022Quote: ... we injected adult male or female mice at approximately 5–6 weeks of age and weighing 18–22 g with 0.07 mL methyl scopolamine (i.p., 0.5 mg/mL diluted in sterile saline; Sigma). After 30 min ...
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bioRxiv - Physiology 2022Quote: ... Nω-nitro-L-arginine methyl ester (10mg/kg, 15 min later, Sigma, USA, N5751-5G, purity>98%), and ioversol injection (8.3 mL/kg ...
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bioRxiv - Immunology 2020Quote: ... water soluble cholesterol–methyl-β-cyclodextrin and L-arginine were obtained from Sigma-Aldrich (St. Louis, MO). Digoxin ...
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bioRxiv - Molecular Biology 2020Quote: ... EATRO 1125 bloodstream forms (pleomorphic) were cultured in HMI-9 media containing 1.1 % methyl cellulose (Sigma, M0512) to maintain their pleomorphism [20] ...
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bioRxiv - Biochemistry 2019Quote: ... The retention time for the method locked using standard mixture of fatty acid methyl esters (Sigma Aldrich).
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bioRxiv - Evolutionary Biology 2021Quote: ... 20 μg/ml catalase and 100 μg/ml glucose oxidase) and 0.25% methyl cellulose (1,500 cP, Sigma). OdinTubulin was similarly polymerized by addition of 0.7 mM GTP in the IRM buffer at the protein concentrations and cation conditions indicated in Fig ...