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Citations for Millipore Sigma :
2251 - 2300 of 10000+ citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... followed by incubating with the primary antibody (mouse monoclonal anti-acetylated tubulin, 6-11B-1, 1:1000, Sigma T6793) and then with the secondary antibody (goat anti-mouse IgG-Alexa Fluor 488 ...
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bioRxiv - Cell Biology 2024Quote: ... we use primary anti-acetylated tubulin antibody from mouse (monoclonal 6-11 B-1, T7451 Sigma, 1/1000 dilution) as well as primary anti-tyrosinated tubulin antibody from mouse (monoclonal TUB-1A2 ...
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bioRxiv - Cell Biology 2022Quote: Cannulated lungs from 17-18-month-old male C57BL/6 mice were filled with warm low melting point agarose (3%, Sigma-Aldrich, St. Louis, MO). Lung segments were cooled on ice for 30 min to allow solidification of the agarose ...
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bioRxiv - Immunology 2023Quote: ... 300,000 WT T84 cells per well in a 6-well plate were treated with 20 µL lentivirus using 3 µL Polybrene transfection reagent (Sigma Aldrich #TR-1003-G) diluted in 3 mL media ...
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bioRxiv - Plant Biology 2022Quote: ... 20 mM Tris–HCl [pH 8.0], 5 mM imidazole, 5% glycerol, 1 mM PMSF, 1/10 protein inhibitor cocktail: Sigma, St. Louis, MO, USA). After centrifugation for 40 min at 21,000 xg ...
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bioRxiv - Neuroscience 2022Quote: Sympathetic nervous ablation was performed with 6-hydroxydopamine hydrobromide (6-OHDA, Sigma, H116). Universal ablation of sympathetic nerves was performed in mice by i.p ...
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bioRxiv - Plant Biology 2023Quote: ... plants were sprayed with 100 μM 6-benzyl purine (6-BAP, Sigma-Aldrich) or Mock solution 24h prior to analysis ...
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bioRxiv - Bioengineering 2021Quote: ... The CGCaP composites were then crosslinked via carbodiimide chemistry in a PBS solution of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, Sigma-Aldrich) and N-hydroxysulfosuccinimide (NHS ...
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bioRxiv - Bioengineering 2020Quote: ... The film crosslinking level was controlled by incubating the coated scaffolds in 30 or 70 mg/mL 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma, France). The film crosslinking level has an effect on the film stiffness as shown in previous studies [23] ...
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bioRxiv - Bioengineering 2020Quote: Crosslinking of scaffolds were prepared by rehydrating scaffolds in PBS overnight with 1-ethyl-3-(3- dimethylaminopropyl) carbodiimide (EDAC, Sigma-Aldrich) and N-hydroxysuccinimide (NHS ...
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bioRxiv - Microbiology 2021Quote: ... RNAs were then chemically cross-linked to the membrane during 90 min at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Sigma Aldrich). Membranes were prehybridized for 30 min in PerfectHyb™ plus (Sigma Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... and Grace Bio-Labs reusable CultureWellTM gaskets (3 mm x 1 mm, well capacity 3-10 ul, Sigma-Aldrich, GBL103250-10EA) were cleaned 3 times sequentially in 100% isopropanol and Milli-Q H2O ...
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bioRxiv - Molecular Biology 2022Quote: ... in 0,5× TBE buffer for 1h at 350 mA of current and EDC-cross-linked for 45 min at 60°C with a solution containing 33 mg/ml of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) (Sigma Aldrich), 11 ng/ul of 1-methylimidazol and 0.46% of HCl ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with HRP-conjugated secondary antibodies (Supplementary Table 1) in 3% dried milk (Marvel) or 3% BSA (Sigma Aldrich) at room temperature for 1 h ...
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bioRxiv - Immunology 2023Quote: ... The immobilized liposomes were completely removed with an injection of 40 mM 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS) (Sigma-Aldrich) (20 μl ...
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bioRxiv - Molecular Biology 2023Quote: ... RNAs were chemically cross-linked to the membrane for 90 minutes at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Merck, Sigma Aldrich). The membrane was pre-hybridized for 30 minutes in Perfect Hyb plus (Merck ...
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bioRxiv - Plant Biology 2024Quote: ... where 10 seedlings each were incubated 2.5-3 h in the dark at RT with agitation in 3 ml of liquid medium (same as above) supplemented with either 3 µl of 1 mM Concanamycin A (Sigma, C9705) dissolved in DMSO or with 3 µl DMSO alone (control) ...
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bioRxiv - Neuroscience 2021Quote: ... 6; MgCl2, 4; CaCl2, 5; sucrose, 160; glucose, 25; Hepes, 10; pH 6.7, 500 mOsmol; all chemicals from Sigma-Aldrich, Lyon, France), to avoid desiccation of the brain surface ...
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bioRxiv - Neuroscience 2022Quote: ... mice aged 5 – 6 weeks and harboring the Fezf2-CreER allele were treated with 50 mg/kg body weight tamoxifen (Sigma-Aldrich,T5648) by oral gavage for 5 consecutive days ...
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bioRxiv - Zoology 2019Quote: The midgut tissue dissected out from continuous toxin-exposed susceptible and toxin exposed insects for 6 generations (n=5) were processed for RNA isolation using TRI reagent TM (Sigma Aldrich, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and added dropwise to HEK293T cells that were seeded onto 10-cm dishes a day prior at a density of 5-6 × 106 cells in 10 ml of fresh DMEM with 10% heat inactivated FBS (Sigma-Aldrich #14009C). 18hrs after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... Digested pancreata were washed with HBSS +0.1% BSA and subjected to discontinuous density gradient using histopaque (6:5 Histopaque 1119:1077; Sigma, St. Louis, MO). The islet layer (found at the interface ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were incubated at 37 °C and 5% CO2 for 24 hrs and selected with growth medium containing 6 μg/mL puromycin (Sigma-Aldrich, P8833) and 750 μg/mL neomycin (Thermo ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were selected with 5 µg/ml blasticidin S and surviving colonies were isolated using 6 mm Pyrex® cloning cylinder (Sigma-Aldrich). Gene knockout was verified by western blotting of whole cell lysates with appropriate antibodies.
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bioRxiv - Genetics 2021Quote: ... 3 mM MgCl2 (Sigma, 7786-30-3), 0.1% Tween-20 (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... and EIF4E2 shRNA#3 (sh4EHP#3) (Sigma,). GIGYF2 shRNA#1 (shGIGYF2#1 ...
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bioRxiv - Cancer Biology 2022Quote: ... covered with 3-aminopropyltrimethoxysilane (3-APTS, Sigma) for 3 min for activation ...
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bioRxiv - Neuroscience 2021Quote: ... oxygenated DAB (3-3’-diaminobenzidine, Sigma-Aldrich) was dissolved in 0.1 N HCl at a concentration of 5.4 mg/ml and subsequently diluted ten-fold into sodium cacodylate buffer (pH 7.4 ...
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bioRxiv - Bioengineering 2022Quote: ... 3-mercaptobenzoic acid (3-MBA, Sigma-Aldrich)-capped gold nanoclusters (3-MBA-Au nanoclusters ...
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bioRxiv - Immunology 2021Quote: ... 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium (BCIP /NBT) liquid substrates for AP-enzyme (SIGMA) were added for overnight incubation at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed 3 times and secondary staining was done in permeabilization buffer supplemented with 5% Donkey serum (Sigma) for 1 hour ...
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bioRxiv - Cell Biology 2019Quote: ... Slides were washed 3 times in PBS for 5 min and incubated in DAPI (Sigma Aldrich, Cat. No.10236276001) for nuclei counterstaining ...
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bioRxiv - Immunology 2019Quote: ... The experiment was initiated by adding 3 µCi [9,10−3H]-palmitic acid complexed to 5% BSA (lipids free; Sigma) and ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked for 3 hr in 5% milk in TBS (50 mM Trizma base and 150 mM NaCl, PH 8.3, both Sigma) plus 0.05% Tween-20 (Sigma ...
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bioRxiv - Physiology 2020Quote: The reduction of yellow tetrazolium salt 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma-Aldrich, Germany) was used to measure cellular metabolic activity as a proxy for cell viability [19,22] ...
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bioRxiv - Immunology 2019Quote: ... Embryos were grown at 28.5°C and kept under anesthesia with egg water containing 0.02% buffered 3-aminobenzoic acid ethyl ester (Tricaine, Sigma) during bacterial injections ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 40ul of 20mg/mL X-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside – Sigma Aldrich B44252) and 40ul of 100mM IPTG (isopropyl β-D-1-thiogalactopyranoside – AppliChem A4773 ...
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bioRxiv - Cancer Biology 2021Quote: ... gRNA sequence: 5’-ACTTGGCCAAGGAGTACGG-3’) in line with GFP from the (p04) U6-gRNA:CMV-eCas9-2a-tGFP plasmid (Sigma). One day later ...
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bioRxiv - Microbiology 2021Quote: Cells transfected with NL-43 or pNL4-3-△5’ were lysed using freshly made lysis buffer (0.5% Igepal CA-630, Sigma; 50 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... the slides were washed 3 times for 5 mins while gently shaking at RT with PBS + 0.1% Tween (Sigma) and stained for 90 mins in blocking buffer with a combination of secondary antibodies ...
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bioRxiv - Plant Biology 2022Quote: ... Br-3’,5’-cAMP (B5386) and Br-adenosine (B6272) were purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA). Br-2’,3’-cAMP was purchased from Biolog (Biolog Life Science Institute ...
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bioRxiv - Microbiology 2022Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, San Louis, MI, USA) solution in PBS was added to the cells and incubated for an additional 4 h ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed twice for 10-m in 1x PBS and then incubated for 1-hr in a blocking buffer containing 3-5% Normal Goat Serum (Jackson Immuno Research, 005-000-121) and 0.01-0.03% Triton-X (Sigma, X100), made up in 1x PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... The unbroken cells were removed by centrifugation (3,000 x g; 4°C; 5 min; Sigma 3-16KL; rotor 11180). The membrane fraction was pelleted down by high-speed centrifugation (100,000 x g ...
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bioRxiv - Biophysics 2019Quote: ... The eluate was incubated for 3 h at 4°C with 5 mL of FLAG M2 Agarose beads (Sigma), collected in a glass column ...
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bioRxiv - Neuroscience 2022Quote: ... N2a NM-HAagg (EP) donor cells were treated for 3 d with methyltransferase inhibitors 5-Azacytidine (Aza) (Sigma-Aldrich), Decitabine (Dec ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 250 µL X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) (20 mg/ml) (Sigma-Aldrich, UK) and Isopropyl ß-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated with 5 mg/mL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma Aldrich) in PBS for 15 min at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... and gravity-sedimented for 3-5 min onto coverslips coated with poly-D lysine (Sigma-Aldrich, catalog number P1024), until cells became in contact with the glass ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were broken by vortexing (3 cycles, 5 min each) using 0.2 ml of glass beads (425-600 μm; Sigma)) ...