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Citations for Millipore Sigma :
2251 - 2300 of 7955 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 40 μm brain sections were washed 3 times with 0.3% Triton X-100 (Sigma-Aldrich) in 1X PBS (PBST ...
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bioRxiv - Cell Biology 2020Quote: ... PLA was performed using Duolink® PLA Fluorescent Detection (red) with anti-mouse PLUS and anti-rabbit MINUS oligonucleotides (Sigma-Aldrich). PLA was performed as described elsewhere (Lin et al. ...
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bioRxiv - Cell Biology 2022Quote: ... followed by probing with horse radish peroxidase (HRP)-conjugated α-mouse (1:10000) (Santa-Cruz Biotech) and detection of signal after chemiluminescent HRP substrate (Millipore) treatment.
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bioRxiv - Neuroscience 2019Quote: ... Sections were then rinsed four times in GBA and once in GBB (100mM Tris pH 9.5, 100mM NaCl, 100mM MgCl2) before incubating with BCIP/NBT alkaline phosphatase detection substrate (Sigma, cat #B5655), and rinsing in deionized water before cover-slipping in aqueous mounting media.
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bioRxiv - Neuroscience 2019Quote: ... Immunoreactivity was detected using the enhanced chemiluminescence (ECL) Western blot detection system (Amersham Biosciences Europe) and Luminata™ Forte (Millipore corporation) using ImageQuant LAS 500 Gel Documentation System (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... diluted to 1:2000 was used for detection with Sigmafast BCIP/NBT alkaline phosphatase substrate at 1 mg/mL (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... a 1:10,000 anti-rabbit dilution of IgG alkaline phosphatase was used before detection with NBP-BCIP (nitroblue tetrazolium chloride–5-bromo-4-chloro-3′-indolylphosphate; Sigma-Aldrich) as the substrate.
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bioRxiv - Pathology 2021Quote: PLA was performed in cryostat sections (4–6 μm) obtained from frozen samples of human parietal cortex using the Duolink In Situ Detection Reagents Brightfield system (Sigma-Aldrich). This method monitors the coincidence of specific epitopes and has been previously used for monitoring protein phosphorylation (77) ...
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bioRxiv - Cancer Biology 2020Quote: ... LDH release assay was measured in triplicate samples from 50 μL of conditioned media expressing cells using the Cytotoxicity Detection KitPLUS(LDH) from Sigma Aldrich according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Detection of IgA spots was achieved by using anti-IgA-conjugated horseradish peroxidase and developed with 3-amino-9ethylcarbazole (Sigma-Aldrich). Color development was stopped by washing several times with water ...
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bioRxiv - Immunology 2021Quote: ... and then monoclonal anti-6x(HIS)-tag antibody was probed followed by detection with anti-mouse IgG conjugated with alkaline phosphatase (A3562: 1ml Sigma, USA) was used for developing the blots.
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bioRxiv - Cancer Biology 2022Quote: ... The resulting circle DNA can be amplified (1 h 40 min amplification at 37 °C, Duolink In Situ Detection Reagents Green, Sigma-Aldrich) and visualized by fluorescently labeled complementary oligonucleotide probes incorporation ...
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bioRxiv - Microbiology 2022Quote: ... Detection was performed by colorimetry using nitro blue tetrazolium chloride / 5-bromo-4-chloro-3-indolyl-phosphate (NBT-BCIP, Sigma-Aldrich) as a substrate.
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bioRxiv - Systems Biology 2022Quote: ... cells were transfected 48 hours prior to irradiation using Lipofectamine 3000 per manufacturer’s instructions prior to fixation and detection of phosphorylated histone variant H2AX using indirect immunofluorescence with anti-γH2AX (clone JBW301, Millipore Sigma). For IR-induced Rad51 foci ...
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bioRxiv - Cancer Biology 2024Quote: ... Beads were then resuspended in Laemmli buffer and boiled at 95 °C for 5 min prior to SDS-PAGE and immunoblot analysis using FLAG antibody covalently conjugated to horse radish peroxidase for detection of precipitated L22 (A8592, Sigma-Aldrich).
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bioRxiv - Developmental Biology 2022Quote: ... Specific protein detection was performed using diluted primary antibodies as follows: anti-NDRG1 @ 1:5000 (Sigma-Aldrich, catalog# HPA006881, rabbit polyclonal); anti-GAPDH @1:5000 (GeneTex ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5 μL time points were taken and quenched with 2 mM EDTA then brought to 20 μL final volume for detection using 1:500 dilution anti-H3K4me3 antibody (05-745R, EMD Millipore), 1 nM LANCE Ultra Europium anti-rabbit IgG antibody (PerkinElmer AD0082) ...
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bioRxiv - Microbiology 2023Quote: ... Equal amounts of DRM and DSM fractions were analyzed by SDS-PAGE followed by Coomassie staining and Western blotting for detection of IsdF-3xFLAG using an anti-FLAG M2 antibody (Sigma, #F3165) and infrared imaging (Odyssey CLx ...
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bioRxiv - Cell Biology 2023Quote: ... PLA was performed using Duolink® PLA Fluorescent Detection (red) with anti-mouse PLUS and anti-rabbit MINUS oligonucleotides (Sigma-Aldrich). PLA was performed as described elsewhere (Lin et al. ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... PLA was performed using Duolink® In Situ PLA® Probes and Duolink® In Situ Detection Reagents FarRed (Sigma-Aldrich). The nuclei of ChP samples were stained using Duolink® In Situ Mounting Medium with DAPI (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µL of cell culture supernatant was mixed with 50 µL detection reagent and incubated 20 min at room temperature in Greiner white 96-well microplates (Sigma-Aldrich). Luminescence was measured in triplicate on a GloMax plate reader ...
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bioRxiv - Cell Biology 2023Quote: Proximity Ligation Assay (PLA) was performed in MCF10A cells using the DuoLink® in situ Orange detection reagent (DUO92102, Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The upper part was used for protein detection using a specific monoclonal antibody against Cas9 (1:1000 dilution, #MAC133, Sigma-Aldrich) and Alexa555 secondary antibody for fluorescence detection (iBright ...
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bioRxiv - Bioengineering 2022Quote: ... Anti-fluorescein-AP Fab fragment antibody (cat N° 11426338910) for the detection of fluorescein-labeled lectin was obtained from Sigma-Aldrich. FITC-Concanavalin A lectin was purchased from Vector Laboratories ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Immunology 2024Quote: ... was used as secondary detection antibody incubated at 45 mins at 37°C before addition of para-nitrophenylphosphate substrate (Sigma Aldrich). Plates were read at an optical density (OD ...
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bioRxiv - Cancer Biology 2024Quote: ... we probed the primary antibodies using Duolink PLA probes rabbit PLUS and mouse MINUS at 37 °C for 1 h and then used Duolink In Situ Detection Reagents Red (Sigma Aldrich) to detect the proximity between the proteins of interest ...
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bioRxiv - Cell Biology 2024Quote: ... The proximity ligation and polymerase amplification steps followed the manufacturer’s protocols (Duolink® In Situ Detection Reagents Red, DU092008, Sigma-Aldrich). Note that the 5× ligase and 5× polymerase were diluted and added immediately before addition to the samples ...
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bioRxiv - Cell Biology 2024Quote: ... reagents (In-Situ PLA Probe Anti-Mouse MINUS, Sigma DUO92004; In-Situ PLA Probe Anti-Rabbit PLUS, Sigma DUO92002; In-Situ Detection Reagents FarRed, Sigma DUO92013) according to the manufacturer’s in situ fluorescence protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Aggregated hASYN PLA was performed using a “direct” detection method that involved conjugation of plus and minus PLA probes to anti-hASYN (Syn211; Millipore, RRID:AB_310817) using the Duolink Probemaker kit (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... with levels for each clone determined by immunoblotting using a blend of monoclonal antibodies for the detection of VP1 (MAB8566, Sigma-Aldrich). For VLP production ...
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bioRxiv - Microbiology 2024Quote: ... Gradients were fractionated by manual collection from the top and fractions containing VLPs were identified by western blot analysis using a blend of monoclonal antibodies for the detection of VP1 (MAB8566, Sigma-Aldrich).
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bioRxiv - Microbiology 2024Quote: This assay was performed as described29 by modifying the Duolink Proximity Ligation Assay (Duolink In Situ Detection Reagents Orange, Sigma Aldrich). Cells were seeded on coverslips prior to infection then fixed at 18 hours post infection in 4% paraformaldehyde for 15 min ...
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bioRxiv - Biophysics 2021Quote: ... the cells were collected and blocked with 5% BSA in PBS at RT for 15 min and incubated with primary anti-Flag antibody (1:300, Sigma-Aldrich) at RT for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Inserts were then washed 3 times for 5 min in 0.3% TritonX (Simga; T8787) in PBS and blocked for 1 hr at RT in 5% normal donkey serum (NDS, Millipore; S30), 1% BSA 0.3% TritonX in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... washed three times for 5 min in PBT (PBS with 0.5% Triton X-100) and blocked for 30 min at RT in 10% normal goat serum in PBT (Sigma, cat# G9023). Samples were incubated with the primary antibodies in blocking solution ...
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bioRxiv - Developmental Biology 2021Quote: ... specimens were dehydrated in an ethanol series at RT and stored in 70% ethanol at 4°C and were embedded in Araldite resin (Sigma-Aldrich). Semithin (1 μm ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The plates were incubated for two hours at RT and 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) substrate solution (Sigma-Aldrich, USA) was added for colour development ...
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bioRxiv - Neuroscience 2021Quote: ... Hoechst counterstain was applied (Hoechst 33342 at 1:1000 for 30 min in PBS at RT) prior to mounting the slice using fluoromount (Sigma-Aldricht).
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bioRxiv - Biophysics 2022Quote: ... was incubated with oligomeric CPB at a concentration of around 1.5 mg/ml for 1h at RT and detergent was removed with Amberlite® XAD®-2 biobeads (Sigma) over night at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Brain slices were permeabilized with 1% Triton X-100 (1 h, RT) and then incubated in 0,1% Triton X-100 and 1% bovine serum albumin (BSA, Sigma-Aldrich, A7906) for 1,5 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were stained with DAPI for 1 hr at RT and placed on a glass slide with a coverslip and DABCO mounting media (Sigma Aldrich), which was sealed with nail polish ...
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bioRxiv - Neuroscience 2021Quote: ... washed as above described and re-blotted using mouse anti-β-actin antibody (Sigma-Aldrich, USA; 1:2500, 1 h, RT). Again ...
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bioRxiv - Neuroscience 2021Quote: ... the slides were blocked with 10% FBS in PBST for 1 hour at RT followed by overnight incubation at 4°C with either anti-CRH (Sigma, UK), anti-SOM (Merck Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by washing with 0.3% Triton X-100 in PBS and then dried for 20 minutes at RT and mounted on silane pre-coated slides with FluoroshieldTM with DAPI (Sigma-Aldrich). Sections were visualized and photographed using a Zeiss Axio Imager Z1 microscope equipped with an Axiocam MR3.0 camera (Carl Zeiss ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies were diluted in blocking solution and added to samples for 1 hour at room temperature (RT): ACTN1 (Sigma, A7811), TAGLN (Abcam ...
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bioRxiv - Immunology 2022Quote: ... Real time RT-qPCR was performed using PerfeCTa SYBR Green FastMix Reaction Mix (Green Fastmix, ROX™) (QuantaBio) and KiCqStart primer sets (Sigma). For tissue conditioned media (TCM ...
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bioRxiv - Immunology 2022Quote: ... Real time RT-qPCR was performed using PerfeCTa SYBR Green FastMix Reaction Mix (Green Fastmix, ROX™) (QuantaBio) and KiCqStart primer sets (Sigma).
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bioRxiv - Genomics 2020Quote: ... Subsequently sections were incubated for 1h at RT in PBS blocking buffer containing 5% horse serum (Sigma Aldrich, Cat. N: H0146), 0.3% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Genomics 2020Quote: ... in situ cDNA synthesis reaction was performed by the addition of 75μl RT reagents: 50ng/μl actinomycin D (Sigma-Aldrich, USA), 0.5mM dNTPs (Thermo Fisher Scientific ...