Labshake search
Citations for Millipore Sigma :
2101 - 2150 of 7557 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... then washed three times in DBPS containing 1% (wt/vol) bovine serum albumin (BSA; Sigma). Bleached oocysts were stored for up to 1 week in DBPS plus 1% BSA at 4 °C before infection ...
-
bioRxiv - Cell Biology 2023Quote: ... washed 3 times for 5 min in PBS and permeabilized with 0.5% NP-40 (Sigma) in PBS for 5 min ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells were then rinsed three times with 0.5% bovine serum albumin (BSA; Millipore Sigma: 126593) before permeabilization in 0.1% Triton X-100 (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2023Quote: ... blood samples were diluted 500 times in the buffer and thiazole orange (Sigma-Aldrich, USA) was added ...
-
bioRxiv - Biochemistry 2022Quote: ... MEF cells were washed three times with PBS buffer (Dulbecco’s Phosphate Buffered Saline - Sigma-Aldrich) and incubated for additional 24 h in DMEM/High Glucose (51449C ...
-
bioRxiv - Plant Biology 2023Quote: ... were pre-treated with the pharmacological agents for different time intervals: 1.5 mM LaCl3 (Sigma) and 2 mM EGTA (Sigma ...
-
bioRxiv - Bioengineering 2023Quote: ... The gels were then rinsed 3 times with 0.1% Tween 20 (Sigma-Aldrich P9416-100ML) in PBS for 5 minutes ...
-
bioRxiv - Immunology 2023Quote: ... Plates were washed (5 times with PBS-T) and developed with either AP substrate (Sigma) and read at 405 nm.
-
bioRxiv - Microbiology 2024Quote: ... Samples were rinsed three times with 0.1% TFA on an equilibrated microspin Harvard apparatus (Millipore) using a microcentrifuge and eluted with 40% acetonitrile in 0.5% acetic acid followed by the addition of 80% acetonitrile in 0.5% acetic acid ...
-
bioRxiv - Immunology 2024Quote: ... The coated plates were then washed 3 times with PBS-T (Sigma cat. # P3563-10PAK) and incubated overnight with 60 μl of a dilution of H6N2 and 60 µl of 2-fold serial dilution of serum ...
-
bioRxiv - Cell Biology 2024Quote: ... washed three times and permeabilized with 0.5% Triton X-100 (Sigma-Aldrich, Saint Louis, MO), for 20 min ...
-
bioRxiv - Microbiology 2023Quote: ... or co- treatment of cells at the time of infection with 50mM of NAC (Sigma) and the compounds listed in Table S4.
-
bioRxiv - Bioengineering 2023Quote: ... samples were washed three times with PBS and permeabilized with 0.2% Triton X100 (Sigma-Aldrich) for 5 min at room temperature ...
-
bioRxiv - Plant Biology 2024Quote: ... FAME were identified by comparison of their retention times with those of standards (Sigma, US) and quantified by the surface peak method using 15:0 for calibration.
-
bioRxiv - Microbiology 2024Quote: ... ELISpot plates were then washed three times with PBS + 0.1% Triton X-100 (Sigma-Aldrich), and after an additional five washes with PBS ...
-
bioRxiv - Microbiology 2024Quote: ... Membranes were washed 3 times in TBS buffer supplemented with 0.1% TWEEN-20 (Millipore P1379), probed with 1:5,000 dilutions of donkey anti-rabbit IRDye680 (Licor 926-68023 ...
-
bioRxiv - Microbiology 2023Quote: ... the infected cells were washed three times with prewarmed Dulbecco’s Phosphate Buffered Saline (DPBS; Sigma) and refreshed with prewarmed media to remove the extracellular bacteria ...
-
bioRxiv - Microbiology 2023Quote: ... The tailocin samples were diluted 1000 times in sterile PBS buffer pH 7.2 (Sigma-Aldrich) to acheive the optimal concentration for observation ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... coli C wildtype and phage resistant strains were diluted 10 times in Ringer’s solution (Sigma). 50 µL of each 10 times diluted overnight culture (5 x 106 cells ...
-
bioRxiv - Bioengineering 2023Quote: ... Tendons (n = 5/group/time) were then digested with proteinase-K (5 mg/ml) (Sigma) for 18 hours and stored at -20C until further assays could be performed ...
-
bioRxiv - Cell Biology 2024Quote: ... washed three times with 0.1% BSA/PBS before they were incubated with DAPI (Sigma-Aldrich) in 200 μL for 5-10 min on ice and flow sorted on an ARIA I or ARIA III BD sorters using a 70 μm nozzle ...
-
bioRxiv - Immunology 2024Quote: ... washed 3 times with PBS and blocked with 5% bovine serum albumin (Sigma Aldrich, A9647) in PBS for 1 h ...
-
bioRxiv - Cell Biology 2024Quote: ... FAMES were identified by comparing their retention times with commercial fatty acid standards (Sigma-Aldrich) and quantified using ChemStation (Agilent ...
-
bioRxiv - Cell Biology 2024Quote: ... The following day the cells were washed three times with Buffer A (Duolink, Sigma-Aldrich) at room temperature and then incubated with species specific secondary PLA probes (diluted in Duolink diluent solution ...
-
bioRxiv - Cell Biology 2020Quote: ... PLA was performed using Duolink® PLA Fluorescent Detection (red) with anti-mouse PLUS and anti-rabbit MINUS oligonucleotides (Sigma-Aldrich). PLA was performed as described elsewhere (Lin et al. ...
-
bioRxiv - Cell Biology 2022Quote: ... followed by probing with horse radish peroxidase (HRP)-conjugated α-mouse (1:10000) (Santa-Cruz Biotech) and detection of signal after chemiluminescent HRP substrate (Millipore) treatment.
-
bioRxiv - Neuroscience 2019Quote: ... Sections were then rinsed four times in GBA and once in GBB (100mM Tris pH 9.5, 100mM NaCl, 100mM MgCl2) before incubating with BCIP/NBT alkaline phosphatase detection substrate (Sigma, cat #B5655), and rinsing in deionized water before cover-slipping in aqueous mounting media.
-
bioRxiv - Neuroscience 2019Quote: ... Immunoreactivity was detected using the enhanced chemiluminescence (ECL) Western blot detection system (Amersham Biosciences Europe) and Luminata™ Forte (Millipore corporation) using ImageQuant LAS 500 Gel Documentation System (GE Healthcare) ...
-
bioRxiv - Biochemistry 2021Quote: ... diluted to 1:2000 was used for detection with Sigmafast BCIP/NBT alkaline phosphatase substrate at 1 mg/mL (Sigma-Aldrich).
-
bioRxiv - Microbiology 2020Quote: ... a 1:10,000 anti-rabbit dilution of IgG alkaline phosphatase was used before detection with NBP-BCIP (nitroblue tetrazolium chloride–5-bromo-4-chloro-3′-indolylphosphate; Sigma-Aldrich) as the substrate.
-
bioRxiv - Pathology 2021Quote: PLA was performed in cryostat sections (4–6 μm) obtained from frozen samples of human parietal cortex using the Duolink In Situ Detection Reagents Brightfield system (Sigma-Aldrich). This method monitors the coincidence of specific epitopes and has been previously used for monitoring protein phosphorylation (77) ...
-
bioRxiv - Cancer Biology 2020Quote: ... LDH release assay was measured in triplicate samples from 50 μL of conditioned media expressing cells using the Cytotoxicity Detection KitPLUS(LDH) from Sigma Aldrich according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: Detection of IgA spots was achieved by using anti-IgA-conjugated horseradish peroxidase and developed with 3-amino-9ethylcarbazole (Sigma-Aldrich). Color development was stopped by washing several times with water ...
-
bioRxiv - Immunology 2021Quote: ... and then monoclonal anti-6x(HIS)-tag antibody was probed followed by detection with anti-mouse IgG conjugated with alkaline phosphatase (A3562: 1ml Sigma, USA) was used for developing the blots.
-
bioRxiv - Cancer Biology 2022Quote: ... The resulting circle DNA can be amplified (1 h 40 min amplification at 37 °C, Duolink In Situ Detection Reagents Green, Sigma-Aldrich) and visualized by fluorescently labeled complementary oligonucleotide probes incorporation ...
-
bioRxiv - Microbiology 2022Quote: ... Detection was performed by colorimetry using nitro blue tetrazolium chloride / 5-bromo-4-chloro-3-indolyl-phosphate (NBT-BCIP, Sigma-Aldrich) as a substrate.
-
bioRxiv - Systems Biology 2022Quote: ... cells were transfected 48 hours prior to irradiation using Lipofectamine 3000 per manufacturer’s instructions prior to fixation and detection of phosphorylated histone variant H2AX using indirect immunofluorescence with anti-γH2AX (clone JBW301, Millipore Sigma). For IR-induced Rad51 foci ...
-
bioRxiv - Developmental Biology 2022Quote: ... Specific protein detection was performed using diluted primary antibodies as follows: anti-NDRG1 @ 1:5000 (Sigma-Aldrich, catalog# HPA006881, rabbit polyclonal); anti-GAPDH @1:5000 (GeneTex ...
-
bioRxiv - Biochemistry 2022Quote: ... 2.5 μL time points were taken and quenched with 2 mM EDTA then brought to 20 μL final volume for detection using 1:500 dilution anti-H3K4me3 antibody (05-745R, EMD Millipore), 1 nM LANCE Ultra Europium anti-rabbit IgG antibody (PerkinElmer AD0082) ...
-
bioRxiv - Microbiology 2023Quote: ... Equal amounts of DRM and DSM fractions were analyzed by SDS-PAGE followed by Coomassie staining and Western blotting for detection of IsdF-3xFLAG using an anti-FLAG M2 antibody (Sigma, #F3165) and infrared imaging (Odyssey CLx ...
-
bioRxiv - Cell Biology 2023Quote: ... PLA was performed using Duolink® PLA Fluorescent Detection (red) with anti-mouse PLUS and anti-rabbit MINUS oligonucleotides (Sigma-Aldrich). PLA was performed as described elsewhere (Lin et al. ...
-
LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... PLA was performed using Duolink® In Situ PLA® Probes and Duolink® In Situ Detection Reagents FarRed (Sigma-Aldrich). The nuclei of ChP samples were stained using Duolink® In Situ Mounting Medium with DAPI (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2023Quote: ... 50 µL of cell culture supernatant was mixed with 50 µL detection reagent and incubated 20 min at room temperature in Greiner white 96-well microplates (Sigma-Aldrich). Luminescence was measured in triplicate on a GloMax plate reader ...
-
bioRxiv - Cell Biology 2023Quote: Proximity Ligation Assay (PLA) was performed in MCF10A cells using the DuoLink® in situ Orange detection reagent (DUO92102, Sigma-Aldrich) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... The upper part was used for protein detection using a specific monoclonal antibody against Cas9 (1:1000 dilution, #MAC133, Sigma-Aldrich) and Alexa555 secondary antibody for fluorescence detection (iBright ...
-
bioRxiv - Bioengineering 2022Quote: ... Anti-fluorescein-AP Fab fragment antibody (cat N° 11426338910) for the detection of fluorescein-labeled lectin was obtained from Sigma-Aldrich. FITC-Concanavalin A lectin was purchased from Vector Laboratories ...
-
bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... reagents (In-Situ PLA Probe Anti-Mouse MINUS, Sigma DUO92004; In-Situ PLA Probe Anti-Rabbit PLUS, Sigma DUO92002; InSitu Detection Reagents FarRed, Sigma DUO92013) according to the manufacturer’s in situ fluorescence protocol ...
-
bioRxiv - Immunology 2024Quote: ... was used as secondary detection antibody incubated at 45 mins at 37°C before addition of para-nitrophenylphosphate substrate (Sigma Aldrich). Plates were read at an optical density (OD ...
-
bioRxiv - Cancer Biology 2024Quote: ... Beads were then resuspended in Laemmli buffer and boiled at 95 °C for 5 min prior to SDS-PAGE and immunoblot analysis using FLAG antibody covalently conjugated to horse radish peroxidase for detection of precipitated L22 (A8592, Sigma-Aldrich).