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Citations for Millipore Sigma :
2251 - 2300 of 10000+ citations for IL 3 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Wells were washed thrice with PBST and monoclonal primary antibody (anti-his-tagged mouse antibody, 1:5000 diluted, Sigma-Aldrich, USA) was added and incubation was continued for 1 h at RT ...
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bioRxiv - Immunology 2023Quote: ... 293FT were exposed to supernatants containing pMXs-IRES-Blast-STING-His retroviruses and 4 μg/mL polybrene (Millipore, TR-1003-G). Two days post transduction ...
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bioRxiv - Cancer Biology 2023Quote: ... the soluble fraction (obtained by centrifugation with 13000g at 4°C for 30 min) was applied to His-Select Nickel Affinity Gel (Sigma-Aldrich) in presence of 5 mM imidazole for 2h on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... 1.8 mM KCl and 1.25 mM NaHCO3) before they were transferred to dissociation buffer [HBSS supplemented with 5% heat-inactivated fetal bovine serum (HI-FBS, Sigma, F2442) and 10 mM HEPES (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µL from the supernatant and cell lysates were applied on a nylon membrane and two different antibodies (an HRP-conjugated anti-His tag from Qiagen and an HRP-conjugated anti-strep tag from Sigma-Aldrich) were used on separate blots to detect PR3 ...
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bioRxiv - Biochemistry 2024Quote: ... of GST and His tagged proteins were subjected to buffer exchange using Amicon Ultra 0.5 ml 10 kDa cutoff columns (Millipore Sigma, UFC501024) with five sequential rounds of concentration performed by centrifugation at 14000 g and 4 °C for approximately 10 minutes and dilution with “EB base” (5x ...
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bioRxiv - Biochemistry 2024Quote: ... Ubiquitin was expressed as an untagged protein and then purified and labelled with Cy3 as per ref53.TRIB1 C-terminus (345-372) was first purified using Ni2+ affinity resin (His-Select resin (Sigma-Aldrich) before being dialysed into PBS pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... placed in digestion media containing 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: pDest17 plasmids containing an N-terminal 6x-His tag and Flag-tagged ARH3 wildtype or H182R were transformed into Rosetta (DE3)pLysS Competent Cells (Sigma, #70956). An initial 5 mL culture was grown overnight in Lennox LB broth supplemented with 100 μg/mL of ampicillin and 25 μg/mL of chloramphenicol at 37 °C while shaking at 200 rpm ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% human serum AB (Merck Sigma-Aldrich), 300 IU/ml IL-2 ...
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bioRxiv - Immunology 2021Quote: ... and then treated with human complement serum (33.3%, Sigma) at 37oC for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg/ml human insulin (Sigma-Aldrich, Gillingham, UK), 0.4 μg/ml hydrocortisone ...
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bioRxiv - Microbiology 2020Quote: ... the working volume of human serum (Sigma-Aldrich, Australia) was divided in half ...
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bioRxiv - Immunology 2021Quote: ... anti-human Caspase-1 antibody (06-503, Merck Millipore); anti-mouse IL-1β antibody (5129-100 ...
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bioRxiv - Genetics 2021Quote: ... 10 μg ml-recombinant human insulin (Sigma, 19278-5ML), 2 IU ml-heparin (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... supplemented with 1.25 mg/ml human insulin (Sigma #I9278), 1 % Penicillin-Streptomycin (Sigma #P4333 ...
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bioRxiv - Neuroscience 2022Quote: ... human basic fibroblast growth factor (bFGF) (Sigma, cat. #F0291), HEPES 1M (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 ng/mL of human bFGF (Sigma-Aldrich). Cells were incubated at 37 °C in a humidified atmosphere with 5% of CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... Normal Human Dermal Fibroblasts (NHDFs) (Sigma-Alrdrich, Buchs, Switzerland), a non-cancerous human skin fibroblast cell line ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10 % heat-inactivated pooled human serum (Sigma) and 100 ng/ml macrophage colony stimulating factor (R&D systems) ...
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bioRxiv - Immunology 2021Quote: ... Anti-human IgG (Fab specific) – peroxidase-conjugated antibody (Sigma) diluted 1:5000 in blocking buffer was added to the wells and incubated for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... human Collagen IV from Sigma-Aldrich (St. Louis, MO), recombinant human Tenascin C ...
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bioRxiv - Cell Biology 2020Quote: ... or shRNA directed against human IP6K1 (TRC0000013508, Sigma-Aldrich) were co-transfected with VSV-G and psPAX2 (a gift from Didier Trono ...
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bioRxiv - Biochemistry 2019Quote: ... 1 µg human GST-Rb (48-378 Sigma Aldrich) as substrate and 0.2 mM ATP ...
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bioRxiv - Bioengineering 2019Quote: ... Human laminin (pepsinized; Millipore Sigma, Burlington, MA, Catalog #AG56P), human tenascin-C (Millipore Sigma ...
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bioRxiv - Immunology 2019Quote: ... with 5% human serum (Sigma-Aldrich; H5667 or H4522), 1% penicillin/streptomycin (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2019Quote: ... and mouse anti-human nuclei (clone 235-1, Millipore) was performed on sections at differing depths of the tumor ...
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bioRxiv - Microbiology 2019Quote: ... were incubated with 25% human immune serum (Sigma-Aldrich) for 30 min ...
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bioRxiv - Bioengineering 2019Quote: Human hepatic stellate cells (LX-2s [34], Millipore Sigma) were used between passages 6-8 for all experiments ...
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bioRxiv - Microbiology 2021Quote: Human neuronal progenitor cells (EnStem-A™ SCC003, Millipore) were cultured on plates previously cultured with geltrex (A1413201 ...
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bioRxiv - Immunology 2020Quote: ... with 5% human serum (Sigma-Aldrich; H5667 or H4522), 1% penicillin/streptomycin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in human serum from male AB plasma (Sigma) and incubated for 16 h at 37 □C with shaking (180 rpm) ...
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bioRxiv - Bioengineering 2020Quote: Human monocytic THP-1 cells (Sigma Aldrich, lot# 16K052) were cultured and expanded in suspension at a cell density of (0.5–1.5)·106 cells·ml−1 culture medium (RPMI-1640 containing 10 % FBS and 1 % P/S ...
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bioRxiv - Bioengineering 2020Quote: ... or human hemoglobin (all proteins were purchased from Sigma). Protein nanorods were prepared using chemically modified human serum albumin.
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bioRxiv - Neuroscience 2021Quote: ... human leukemia inhibitory factor (hLIF, 10 ng/ml, Millipore), SB431542 (2 mM) ...
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bioRxiv - Microbiology 2021Quote: ... rabbit anti-human actin (Sigma cat#A2066, 1:2,000), and human anti-S Serum derived from a convalescent individual (1:1000) ...
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bioRxiv - Biophysics 2020Quote: ... ErbB2 (Her2) human recombinant was purchased from Sigma-Aldrich. Deglycosylated trastuzumab was obtained by treatment with Endo S (New England BioLabs ...
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bioRxiv - Cell Biology 2021Quote: ... HT medium containing 20 nM human EGF (Sigma-Aldrich) was added to the cells at a final concentration of 10 nM (1 ml of 20 nM EGF in HT medium was added to the glass-bottom dish containing 1 ml HT medium) ...
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bioRxiv - Cell Biology 2021Quote: ... followed 5 IU of human chorionic gonadotropin (hCG) (Millipore) 48 hrs later ...
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bioRxiv - Cell Biology 2021Quote: ... Vascular Endothelial Growth Factor human (VEGF Sigma, Cat. # V7259) was injected in the duct of Cuvier ...
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bioRxiv - Cell Biology 2021Quote: ... 10 nM recombinant human [Leu15]-gastrin I (Sigma G9145), 50 ng/ml recombinant mouse EGF (Peprotech ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10 % heat-inactivated pooled human serum (Sigma) and 40 ng/ml macrophage colony stimulating factor (R&D systems) ...
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated goat anti-human IgG Fc (Sigma-Aldrich) was used for detection ...
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bioRxiv - Microbiology 2021Quote: ... conidia were opsonized with normal human serum (Merck Millipore). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... or goat anti-human IgG phycoerythrin conjugate (Sigma, P9170) in PBST-SK was added to the wells ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5 μg/mL human placenta laminin (Sigma-Aldrich) for the epithelial channel ...
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bioRxiv - Microbiology 2020Quote: ... anti-human Fc-conjugated to alkaline phosphatase (A9544, Sigma) was added (50 µl/well ...
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bioRxiv - Microbiology 2019Quote: ... The Human Cytokine/Chemokine Magnetic Bead Panel kit (Millipore) was used according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... Human cerebral microvascular endothelial cells (hCMEC/D3, Sigma-Aldrich) were maintained in culture using Endothelial Basal Medium (EBM-2 ...
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bioRxiv - Neuroscience 2022Quote: ... human leukemia inhibitory factor (hLIF, 10 ng/ml, Millipore), SB431542 (2 mM) ...