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Citations for Millipore Sigma :
2251 - 2300 of 4275 citations for 6 TERT BUTYLQUINOLINE 98.0+% GC T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Wells were washed twice with PBS to remove T cells and the number of remaining target cells was determined by culturing with 10 μg/mL resazurin (R7017, Sigma) and measuring fluorescence signal in a plate reader ...
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bioRxiv - Neuroscience 2022Quote: ... Cmpd101 effect for SSF-DOR and SSF-DOR S/T to A was assessed as described for SSF-MOR except the agonist used was DADLE 10μM (Sigma-Aldrich). To monitor the lack of cross tolerance between SSF-DOR and SSF-MOR ...
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bioRxiv - Microbiology 2022Quote: ... Jurkat T-cells were cultured in Roswell Park Memorial Institute Medium (RPMI 1640; Catalog no. R4130; Sigma, St. Louis, MO) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μg of FhNEJ-Teg or recombinant protein in the presence or absence of 15 ng t-PA (Sigma) or 10 ng u-PA (Sigma) ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were then incubated overnight at 4 °C with 10 ml TBS-T containing either anti-c-myc-peroxidase antibody (1:10,000; Sigma, A5598), or anti-HA primary antibody (1:10,000 ...
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bioRxiv - Plant Biology 2022Quote: ... Anti-HA gels were washed 3 times with 20 ml TBS-T and then incubated with peroxidase-conjugated secondary antibody (1:10,000; Sigma A0545) in TBS-T for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated overnight at 4°C with PBS-T containing 2% NGS and antisera against Olig-2 (1:500,Millipore) A2B5 (which stains for OPCs ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were then washed for 10 minutes in PBS at room temperature and blocked for 1 hour in PBS with 0.1% Triton-X100 (PBS-T, Sigma Aldrich) and 1.5% donkey serum (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were washed with TBS-T and membranes were incubated with secondary antibodies anti-mouse and anti-rabbit coupled to alkaline phosphatase (1:5000; Sigma) diluted in TBS-T/1% BSA for one hour at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... sections were fixed with ice-cold 100 % acetone for 10 min at −20 °C and washed with PBS-T (0.1 % v/v Tween 20, Sigma-Aldrich) before blocking with 5 % BSA in PBS (m/v ...
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bioRxiv - Molecular Biology 2022Quote: ... for one hour and incubated overnight at 4°C in 5% milk/PBS-T with the following antibodies: anti-LIMCH1 (1:400, catalog number HPA063840, Sigma), anti-MBNL1 (1:1,000 ...
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bioRxiv - Immunology 2024Quote: ... Fetuin-coated plates were then washed 3 times with PBS-T and incubated for 1 h with a solution of PNA-HRP (cat. # L6135-1MG, Sigma) at 5 μg/ml in conjugate diluent (MES pH 6.5 ...
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bioRxiv - Plant Biology 2024Quote: ... samples were processed the same way except that membranes were blocked in 1% (w/v) BSA in TBS-T and incubated in 1:1,000 streptavidin-HRP (Sigma, S2438).
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bioRxiv - Molecular Biology 2024Quote: ... incubated as necessary with primary antibody diluted in PBS-T containing 1% or 2.5% bovine serum albumin (immunoglobulin- and lipid-free fraction V, Sigma-Aldrich) and washed 3 times with PBS-T ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% Tween-20 (TBS-T) and incubated for one hour at RT with Mouse anti-FLAG (M2) antibody-HRP conjugated (Sigma) diluted 1:4000 in blocking milk ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were then washed with TBS-T 0.1% three times and developed with Immobilon Forte Western HRP Substrate (Millipore, WBLUF0500). All blots were imaged via chemiluminescence on a ChemiDoc Touch (cat#12003153 ...
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bioRxiv - Neuroscience 2024Quote: ... Brain slices were incubated overnight at 4°C in the presence of primary antibodies in PBS-T: rabbit PYK2 antibody (1/500, Sigma #07M4755 ...
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bioRxiv - Cell Biology 2024Quote: ... After transfer the blots were blocked in a 5 %(w/v) solution of milk powder in TBS-T (50 mM Tris, 150 mM NaCl, 0.05% Tween 20 (Sigma, P9416)) for 1h at RT ...
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bioRxiv - Cell Biology 2024Quote: Doxycycline-inducible stable Flp-In T-REx HEK293 cell lines expressing mitochondrial-relocated golgin-BirA* fusion proteins were induced with 1 μg/ml doxycycline (Sigma) in culture medium once they reached approximately 80% confluency ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... and analyzed by SDS-PAGE using mouse anti-GFP antibody (Max Perutz Labs, Monoclonal antibody facility) in 3 % (w/v) milk powder TBST-T (Sigma). Secondary antibody incubation was performed using Dylight 800 α-mouse (Rockland Immunochemicals) ...
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bioRxiv - Cell Biology 2023Quote: ... Blots were then washed with TBS-T three times for five minutes and exposed to Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) according to product instructions for 30 seconds before being developed ...
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bioRxiv - Immunology 2023Quote: ... T cell response was determined by cleavage of the chromogenic LacZ substrate chlorophenol red-β-D-galactopyranoside (CPRG, Millipore Sigma) by TCR-induced LacZ and measure by spectrophotometry as presented by absorbance at 595 nm (A595 ...
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bioRxiv - Immunology 2024Quote: ... 1.4×108 human primary CD4+ T cells were transduced with lentivirus at a multiplicity of infection of 2 with 8 µg/ml dextran (Sigma). Virus and DynaBeads were removed after 3 days of transduction ...
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bioRxiv - Immunology 2024Quote: Activated TKO CD4+ T cells were activated with Cell stimulation cocktail plus protein transport inhibitors (eBioscience) and PMA (500 ng/ml, Sigma) for 4 hrs and then incubated with antibodies targeting cell surface markers ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were activated as earlier described and adhered to glass slides coated with poly- L-lysine (Sigma Aldrich). The cells were fixed with 4% paraformaldehyde for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... All membranes were incubated with the indicated primary antibodies diluted in TBS-T (20mM Tris pH 7.5, 150 mM NaCl, 0.1% Tween-20) with 5% bovine serum albumin (Sigma-Aldrich, A7906) overnight at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK (1:1000, Sigma Millipore ...
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bioRxiv - Immunology 2022Quote: ... Wells were washed twice with PBS to remove T cells and the number of remaining target cells was determined by culturing with 10 μg/mL resazurin (Sigma) for 2 hours and measuring fluorescence signal in a plate reader ...
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bioRxiv - Cell Biology 2023Quote: ... animals were injected with N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT) (Sigma, Cat no. D5942) (10 mg/kg/day ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells isolated from healthy donor PBMCs were stimulated by a 1:1 ratio of anti-CD3/28 activation beads (Miltenyi) in complete human T cell medium (RPMI1640 from Sigma containing 2 mM glutamine with the addition of 1 mM pyruvate ...
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bioRxiv - Molecular Biology 2023Quote: ... the FLAG-AGO1 proteins were detected with anti-FLAG-HRP antibody (1:4000 [Sigma] in 5 % milk in PBS-T). The membrane was developed as described above with homemade ECL or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Physiology 2023Quote: ... Following overnight incubation at 4°C with primary antibodies against CARNS1 (rabbit polyclonal, 1:1000 in 3% milk/TBS-T, HPA038569, Sigma), membranes were washed (3 × 5 min) ...
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bioRxiv - Genomics 2022Quote: ... milk powder in TBS-T (Tris-buffered saline-Tween-20) and incubated overnight with primary antibody in 3% BSA/TBS-T (monoclonal mouse anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed a final time (5x with PBS-T) and the plate was allowed to develop by addition of AP substrate (Sigma). Optical density at 405 nm was measured in 5 min intervals ...
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bioRxiv - Cell Biology 2023Quote: ... primary and secondary antibody incubations were done in TBS-T (Roth, 1061.1) supplemented with 5% milk powder (Heirler, 4010318030305) or 5% BSA (Sigma, A7906). Blots were blocked for 1h at RT and primary antibody was added over night at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... CD4+T cells were then stimulated with 100 ng/mL of PMA and 1 µg/mL of ionomycin (both from Sigma) for 12 hrs ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A constant amount of plasmid cDNA (15 μg) was transfected into human embryonic kidney cells 293 T (HEK-293T) using polyethylenimine (PEI; Sigma) in a 1:2 weight ratio in 10 cm plates ...
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bioRxiv - Immunology 2023Quote: ... CD8+ and CD4+ T cells were resuspended in RPMI 1640 with 25 mM Hepes (Sigma-Aldrich, St. Louis, Missouri, USA), supplemented with 10% BCS (Bovine Calf Serum ...
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bioRxiv - Cell Biology 2023Quote: ... Monocyte-depleted PBMCs or isolated CD4+ T cells were stimulated immediately after their respective isolation with PHA (1 µg/mL, Sigma) or Dynabeads® Human T-Activator CD3/CD28 (Thermofisher) ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with 10% skim milk (in 1x TBS-T) for 1 h and probed with anti-GFP antibody (1:1,000; Sigma-Aldrich) in 5 % skim milk (in 1x TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... Ablation of adult M/T cells was induced by intraperitoneal injection of the diphtheria toxin A (DT) subunit (Sigma D0564) at a concentration of 20 μg/Kg body weight into Tbx::iDTR transgenic mice (Dong et al. ...
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bioRxiv - Immunology 2023Quote: ... for 1-2 hours at RT followed by 3 washes in PBS + 0.1% Tween 20 (PBS-T; Sigma P7949-100ML). Primary antibody was then fixed to the tissue for 30 minutes in 4% PFA for 30 minutes at RT followed by 3 washes with PBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... larvae were washed 3 times in PBS-T (phosphate buffered solution + 0.6% Triton-X 100) (Sigma-Aldrich, Cat. No. X100). PBS-T was removed and VECTASHIELD Mounting Media (Vector Labs ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed in TBS supplemented with 0.05% Tween-20 (TBS-T) and blocked in 5% fresh made skim-milk (Sigma-Aldrich) in TBS-T ...
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bioRxiv - Biochemistry 2023Quote: HEK293 Flp-In T-Rex cells were cultured in DMEM high glucose medium supplemented with 10% FBS (Sigma-Aldrich F0804), 2mM L-glutamine (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... Further viral amplifications were performed using adherent Sf9 cells in a T-75 flask containing ESF 921 growth medium plus 5% heat-inactivated FBS (Sigma) at 27 °C ...
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bioRxiv - Neuroscience 2023Quote: ... permeabilised by washing in 0.1% Triton-X in PBS (PBS-T) for 15 min and incubated for 2 h in a blocking buffer of 2% bovine serum albumin (Sigma) and 3% normal donkey serum (Sigma ...
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bioRxiv - Immunology 2023Quote: CD8 T cells were isolated from peripheral blood of donor P14 mice via gradient centrifugation with Histopaque-1083 (Sigma-Aldrich). 500 naïve P14 cells were adoptively transferred intravenously (i.v. ...
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bioRxiv - Immunology 2023Quote: ... The percentage of PI incorporation was calculated based on CD8+ T cells treated with 0.1% Triton X-100 (Sigma Aldrich) for 15 min (= 100 %) ...
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bioRxiv - Cell Biology 2023Quote: ... Flp-In T-REx293 cell lines were maintained in DMEM containing high glucose and pyruvate (Nacalai Tesque) supplemented with 10% FBS (Sigma) and 1% penicillin-streptomycin (Nacalai Tesque) ...