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Citations for Millipore Sigma :
2201 - 2250 of 4275 citations for 6 TERT BUTYLQUINOLINE 98.0+% GC T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... After washing 3–4 X with 1× PBS-T the strips were incubated with 1:5000 mouse anti-GST antibodies (Sigma). The secondary antibodies (anti-rabbit-HRP or anti-mouse-HRP ...
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bioRxiv - Immunology 2021Quote: ... IgG was detected using goat-anti-human-Fc-AP (1:1000) (Jackson, catalogue no. 109-055-098) and wells were washed with PBS-T and AP substrate (Sigma) was added and plates read at 405 nm ...
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bioRxiv - Neuroscience 2020Quote: Human Embryonic Kidney Cells (HEK293) and HEK293 Flp-In T-Rex cells were grown and maintained in Dulbecco’s modified Eagle medium (DMEM, Sigma-Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were washed in Tris-buffered saline-Tween (TBS-T) (20 mM Tris pH 7.4, 150 mM NaCl (both Sigma-Aldrich) and 0.1% Tween 20 (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were blocked in 5% BSA TBS-T (5% BSA in 1x Tris Buffered Saline, 0.1% Tween 20, Sigma Aldrich) for 1 hour and incubated with primary antibodies overnight in 5% BSA TBS-T (MMP1 1:1000 ab137332 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and diroximel fumarate (MW= 255.23) (synthesized by T. Avery) were suspended in methylcellulose (viscosity 15 cP, 2% w/v in water; Sigma-Aldrich). For SNI anti-nociception ...
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bioRxiv - Systems Biology 2021Quote: ... purified LC and naïve CD4+ T cells or TRMs were co-cultured in human serum supplemented media (RPMI, Gibco, UK, 10% human serum, Sigma, UK ...
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bioRxiv - Microbiology 2020Quote: ... Overnight turbid cultures were then diluted 1/50 in an appropriate volume EBB/T or CMM for further experiments with 100 μM levodopa (D9628, Sigma), 5-hydroxytrytophan (H9772 ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed multiple times in TBS-T for 1h before adding secondary antibody (goat anti-mouse alkaline phosphatase conjugate; Sigma) as above at 1:2500 dilution ...
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bioRxiv - Microbiology 2021Quote: ... membranes were blocked for 1 h with 5% dried skim milk in 1X PBS containing 0.1% Tween 20 (T-PBS) and incubated with primary anti-HA epitope tag (Sigma, H6908) or anti-GFP (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... mexicana strain M379 (MNYC/BC/62/M379) promastigotes were grown in T-75 flasks at 26 °C in Schneider’s insect medium (Sigma-Aldrich) supplemented with 0.4 g/L NaHCO3 ...
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bioRxiv - Microbiology 2021Quote: ... Terminal cellular differentiation was induced with 10 μM N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT; Sigma-Aldrich) while mucus-secretion was enhanced with 10 nM phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Microbiology 2019Quote: ... The cells were washed 3 times with PBS containing 0.1% Tween-20 (Sigma Aldrich, Castle Hill, NSW, Australia; PBS-T) before being stained with 400μl AlexaFluor 488-labelled anti-Rabbit IgG (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were treated with 0.25μM testosterone 3-(O-carboxymethyl) oxime:BSA-fluorescein isothiocyanate (T-BSA-FITC) conjugate (T5771, Sigma-Aldrich, Munich, Germany) diluted in Tris-Buffer (pH 7.2 ...
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bioRxiv - Neuroscience 2022Quote: ... Male Cd38flox/flox:GlastCreERT2/+ and Cd38flox/flox:Glast +/+were injected with tamoxifen (20mg/kg; T-5648, Sigma-Aldrich, St. Louis, MO, USA) intraperitoneally once a day for 5 consecutive days from P10 or P42 ...
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bioRxiv - Biochemistry 2022Quote: ... cells were lysed with cell lysis buffer (TBS-T; 20mM Tris-pH8, 160mM NaCl, 1% Triton, 1X protease inhibitor cocktail (Sigma)) ...
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bioRxiv - Neuroscience 2020Quote: ... Cre recombination was induced by oral gavage of pregnant mice with 5 mg of tamoxifen (Sigma-Aldrich, France, T-5648) at embryonic days E10.5 and E11.5(Eon et al. ...
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bioRxiv - Cell Biology 2020Quote: ... were blocked for 1h in TBS containing 0.1% Tween-20 (TBS-T) supplemented by 3% fatty-acid free BSA (Sigma Aldrich) before incubation with the purified STARD10 protein (1 μg/mL in TBS-T + 3% BSA ...
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bioRxiv - Bioengineering 2020Quote: We assessed the differentiation by measuring the fraction of H9c2 cells positive for troponin T by immunofluorescence using the T6277 antibody from Sigma. As this antibody detects both cardiac and skeletal muscle troponin T ...
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bioRxiv - Immunology 2020Quote: ... CSR to IgG3 and IgG1 was induced in T cell independent manner by exposure to 5 μg/ml of Lipopolysaccharide (Escherichia Coli LPS, 055:B5, Sigma) or LPS+rIL-4 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in blocking buffer (5% non-fat milk or 5% FBS diluted in TBS-T buffer (0.05% Tween-20 (Sigma-Aldrich) in tris-buffered saline (TBS) ...
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bioRxiv - Immunology 2022Quote: ... The T cells were resuspended at concentration of 4×106/ml in TCGF with 8 µg/ml polybrene (Sigma, 107689) and this suspension was plated at 500 µl/well in 12-well plates ...
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bioRxiv - Immunology 2022Quote: ... 3x105 CD19+ B cells were added to pre-existing wells containing T cells and supplemented with 200ng/mL BAFF (Sigma) in complete RPMI for 5 days ...
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bioRxiv - Cell Biology 2022Quote: ... Homogenization of samples was obtained using a motorized pestle and 100 µL T-PER (Thermo Fischer Scientific) containing protease inhibitors (Sigma). 30 µg of protein was subjected to standard SDS-PAGE and Western blotting with HFP-conjugated secondary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... assays were performed as previously described (Bruno T, Cancer Cell, 2002) by using anti-acetyl-Histone H3 (Lys27) antibody (Millipore). Equal amounts of precleared chromatin were added to antibody-bound Dynabeads (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2019Quote: PBMCs from healthy donors were processed for isolation of CD4+ CD127low CD25+ Regulatory T cells using Histopaque (Sigma Aldrich, Germany). Briefly ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were washed 3 times with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP (Horseradish Peroxidase) Substrate (Merck Millipore) using a Gel-Doc XR+ System (BioRad) ...
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bioRxiv - Immunology 2019Quote: ... A total of 400,000 freshly isolated splenocytes in T cell media supplemented with 3-5 μg/ml ConA (Sigma Aldrich) were added to 6.5 mm transwell inserts with 0.4 μm pore size polyester membranes (Corning ...
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bioRxiv - Immunology 2020Quote: ... Plates were then blocked with ELISA blocking buffer containing 2% w/v bovine serum albumin in PBS with 0.1% v/v Tween20 (PBS-T) (Sigma-Aldrich) for 1 h at room temperature ...
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bioRxiv - Plant Biology 2019Quote: ... the blot was washed with 1×TBS-T solution (4×5min) and incubated with anti-Mouse IgG-HRP secondary antibody solution (Sigma, 1:10,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche] ...
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bioRxiv - Neuroscience 2019Quote: ... Fibril morphology was confirmed by imaging using a Tecnai G2 Spirit TWIN transmission electron microscope (FEI Company) at 120kV and Thioflavin T (Sigma) fluorescence reading (data not shown) ...
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bioRxiv - Plant Biology 2019Quote: ... The dried non-polar phase was resuspended in 70 μL of pyridine and derivatized with 30 μL of MTBSTFA + 0.01 % t-BDMCS (Sigma-Aldrich). Following transfer to a glass vial ...
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bioRxiv - Immunology 2020Quote: ... After 20 hours co-culture wells were washed 3 times with PBS to remove T cells and the number of remaining target cells was determined by culturing with 10 μg/ml resazurin (Sigma) and measuring fluorescence signal in a plate reader ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) was added ...
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bioRxiv - Neuroscience 2020Quote: ... cells were treated for 16 hours with N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-phenylglycin T-butyl ester (DAPT) (Sigma Aldrich), an inhibitor of γ-secretase ...
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bioRxiv - Neuroscience 2021Quote: ... slices were washed with PBS/0.1% Triton X-100 (T-PBS) and incubated with the primary antibody NeuN (1:200, Sigma, ABN78) in T-PBS for 1 day at 4°C while shaking ...
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bioRxiv - Cell Biology 2021Quote: ... Amyloid was visualized using a 2-minute incubation in Thioflavin S (0.5% w/v; #T-1892, Sigma, St. Louis, MO) followed by a brief wash in 70% ethanol as described previously5 ...
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bioRxiv - Systems Biology 2021Quote: ... the membrane is blocked using 5% milk in TBS-T buffer (Tris-Buffered Saline pH 7.6 [TBS {20 mM Tris pH 8, 150 mM NaCl, Hydrochloric acid [Sigma Aldrich, 320331]}] with 0.1% Tween-20 [Fischer Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Washes with fresh phosphate-buffered saline with Tween 20 (PBS-T) were then performed and PBS with 0.3% Triton X-100 (Sigma, T8787) was applied for 10 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were manually dissociated through a 70µM cell strainer and OTI T-cells were activated and expanded with 20ng/mL of SIINFEKL peptide (Sigma-Aldrich) and 100 IU/mL recombinant human IL-2 (Biolegend ...
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bioRxiv - Microbiology 2020Quote: ... diluted 1:1000 in phosphate-buffered saline (PBS) containing 0.05% Tween 20 (PBS-T) followed by anti-rabbit horseradish peroxidase (HRP)-coupled antibody (Sigma-Aldrich) diluted 1:1000 in PBS-T ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) was added ...
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bioRxiv - Microbiology 2021Quote: ... milk powder in TBS-T (Tris-buffered saline-Tween-20) and then incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG ...
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bioRxiv - Molecular Biology 2020Quote: ... Wells were washed four times with PBS-T and 25 μL of prepared Immobilon Western Chemiluminescent HRP Substrate (Millipore #P90719) added to each well immediately prior to the plate being read on a Synergy Neo2 Plate Reader (BioTek Instruments) ...
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bioRxiv - Microbiology 2021Quote: ... in 1X PBS with 0.1% of Tween 20 (PBS-T) for 1 h and probed with a 1:3,000 dilution of α-FLAG-HRP antiserum (Sigma), 1:1,000 dilution of α-AzuC antiserum (New England Peptide) ...
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bioRxiv - Immunology 2020Quote: ... For in vitro deletion of floxed alleles of Rc3h1fl/fl;Rc3h2fl/fl;CD4Cre-ERT2;rtTA3 (but with no effect on the Cre-deficient WT control Rc3h1fl/fl;Rc3h2fl/fl;rtTA3) CD4+ T cells were treated with 1 μM 4′OH-tamoxifen (Sigma) for 24h prior to activation at a cell density of 0,5-1×106 cells/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked with 5% milk in TBS-T (TBS with 1% Tween-20) and incubated over night at 4°C with the primary anti-FLAG antibody (Sigma F1804-200UG ...
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bioRxiv - Biochemistry 2022Quote: ... Blots were washed an additional three time with TBS-T and one time with TBS before 3 minute exposure with ECL HRP substrate (Millipore) and imaged on a BioRad ChemiDoc system ...
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bioRxiv - Cancer Biology 2022Quote: ... As positive control T cells have been non-specifically stimulated with 0.5 ng/µL phorbol-12-myristate-13- acetate (PMA, Sigma Aldrich) and 1 ng/µL Ionomycin (Sigma Aldrich) ...
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bioRxiv - Genetics 2022Quote: ... Binding of the anti-FLAG M2 primary antibody (mouse, Sigma-Aldrich, F3165, diluted 1:3000 in PBS-T+1%BSA) was allowed in a humidity chamber (overnight ...