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Citations for Millipore Sigma :
2251 - 2300 of 10000+ citations for 6 Amino 2 4 difluorobenzotrifluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... were expressed in 3T3 cells and IPed using 1µg anti-Fyn (mAb, sc-434, SantaCruz) or 4 µg anti-MAP2 (HM-2, mAb, Sigma, Cat # M4403) and Protein G beads (CalBiochem) ...
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bioRxiv - Immunology 2021Quote: ... mice were anaesthetised using inhaled isoflurane (4% (v/v) isoflurane and 2 l O2/min) and 30 μl of LPS (250 μg/mL, Sigma-Aldrich) or vehicle PBS were administered intranasally ...
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bioRxiv - Biochemistry 2021Quote: ... at a flow rate of 10 µL/min for 4 min in an isocratic mode of Mobile Phase C (2% acetonitrile (Sigma-Aldrich), 0.1% formic acid) ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes of primary motor neuron samples were incubated overnight at 4°C with primary antibodies for ERK1/2 (1:10000, Sigma Aldrich) and ATPB (1:1000 ...
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bioRxiv - Biophysics 2020Quote: ... Samco Trident Vial Tall with PP Screw Cap) were incubated for 4 hours with 2 % aqueous Tween solution (Tween20, Sigma-Aldrich), then rinsed with deion ...
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GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... and viral envelope (pMD2G) constructs at a 4:2:1 ratio with 1 mg/mL polyethylenimine (PEI, linear MD 25,000 Da, Sigma, Cat# 408727). The ratio of total transfected DNA to PEI was 1:3 (1 µg DNA:3 µg PEI) ...
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bioRxiv - Biochemistry 2021Quote: MDA-MB-231 cells were lysed with RIPA buffer for 2 h at 4°C and tissues were disrupted and lysed in RIPA buffer (Sigma-Aldrich) using a MagNA Lyser instrument (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... Supernatant protein concentrations were adjusted to 5 mg/mL and incubated for 2 h at 4 °C with 200 μL of anti-FLAG matrix (Sigma-Aldrich) for co-immunoprecipitation with FLAG-RBOHD and with 200 μL of anti-HA magnetic beads (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2021Quote: ... Seeds were surface sterilized and kept for stratification in dark at 4 °C for 2-3 days on half strength MS-agar (Millipore Sigma) plates ...
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bioRxiv - Biophysics 2020Quote: ... maintains a pluripotent state of the stem cells.2–4 Cells were cultured in Glasgow modified Eagle’s medium (GMEM, Sigma Aldrich, Germany) supplied with 5.5 ml non-essential amino acids (Gibco Science ...
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bioRxiv - Biochemistry 2022Quote: ... Valine only labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 150 mg/L sodium 4-methyl-2-oxovalerate (Sigma K0629) ...
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bioRxiv - Biochemistry 2022Quote: ... Dimethyl LV labeling for geminal pair determination used 150 mg/L 2-Keto-(3-methyl-13C)-butyric-4-13C,3-d acid sodium salt (Sigma 589063). Valine only labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887 ...
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bioRxiv - Biochemistry 2022Quote: ... 13C-ILV labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 70 mg/L 2-Ketobutryic acid-4-13C,3,3,d2 sodium salt hydrate (Sigma 589276 ...
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bioRxiv - Microbiology 2022Quote: ... 6-Bromo-4-((dimethylamino)methyl)-5-hydroxy-1-methyl-2-((phenylthio)methyl)-1H-Indole-3-carboxylic acid ethyl ester monohydrochloride (Arbidol) (Sigma Aldrich) was dissolved in ethanol at 10mg/ml and diluted to target concentration in infection media.
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bioRxiv - Pathology 2022Quote: ... 1 part NTA), incubated (30min., RT) and centrifuged (3000g, 1h, 4°C) in an Ultra-2 Centrifugal Filter Unite (Millipore, #UFC203024). Filtered samples (100µL ...
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bioRxiv - Microbiology 2022Quote: ... 3-(N-Morpholino)propanesulfonic acid (MOPS) or 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) buffer (all purchased from Sigma-Aldrich, USA). Macrocolonies were excised and resuspended in 2 mL of sterile 1X PBS ...
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bioRxiv - Bioengineering 2022Quote: ... A 2 μL aliquot of 100 μM aptamer in PBS was thawed and mixed with 4 μL of 10 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich) for 8 hours to reduce the disulfide bond ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.2% Triton X-100 and then incubated overnight at 4°C with a mouse anti-tyrosine hydroxylase antibody (TH, Sigma, T1299) at 1:200 dilution in PBS containing 1.5% BSA and 0.2% Triton X-100 ...
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bioRxiv - Neuroscience 2020Quote: ... Embryos at 2 dpf (days post-fertilization) were removed from their chorions and anesthetized with 4% tricaine (MS-222, Sigma Aldrich). The embryos were placed in a 1% agarose-coated dish with small holes ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL of these extracts were centrifuged at 14,000 xg for 1 min at 4°C with 2.5 μl 2% (v/v) Tween 20 (Sigma-Aldrich, Darmstadt, Germany) to promote the aggregation into a pellet ...
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bioRxiv - Cell Biology 2022Quote: ... Cells from each donor animal were plated as separate cultures in serum-free growth medium42 (SFGM, Supplementary Table 3) on fibronectin-coated (4 μg cm−2 bovine fibronectin; F1141, Sigma-Aldrich) tissue culture vessels for 72 h prior to FACS ...
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bioRxiv - Microbiology 2019Quote: ... metabolic activity by the tetrazolium salt 2,3-bis(2- methoxy-4-nitro-5-sulfophenyl)-5-(phenylamino)-carbonyl-2H-tetrazoliumhydroxidand (XTT; Sigma-Aldrich, USA) reduction assay ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were fixed with 4% formaldehyde for 20 min and stained with 1x crystal violet solution (2% crystal violet (Sigma-Aldrich), 20% ethanol ...
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bioRxiv - Cell Biology 2019Quote: ... The sonicated lysate was incubated for 2 hours at 4 ° C with beads (Glutathione High Capacity Magnetic Agarose Beads, Sigma-Aldrich) previously washed in Rac buffer (50 mM Tris HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... Afterwards the medium was switched and cells were cultivated for 4 days in DMEM containing 10% FCS and 2 μM insulin (Sigma Aldrich) to induce adipogenic differentiation ...
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bioRxiv - Cancer Biology 2020Quote: Non-synchronized and synchronized cells were fixed using 70% Ethanol for 48 hours at 4°C then washed with PBS+2% goat serum (Sigma, UK) and resuspended in 1mL of ice-cold PBS ...
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bioRxiv - Immunology 2020Quote: ... 150 mM NaCl and 0.1% Tween-20 or TBST buffer, 2 h) and incubated (4°C, overnight) with biotinylated BSI-B4 lectin from Bandeiraea (Griffonia) simplicifolia (Sigma-Aldrich, #L3759-1MG ...
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bioRxiv - Molecular Biology 2021Quote: ... Pre-cleared nuclear lysate was then added to 50 μL beads conjugated with 2-4 μg primary IgG antibody: rabbit anti-APOBEC2 (Sigma, HPA017957) or rabbit IgG isotype control ...
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bioRxiv - Plant Biology 2021Quote: ... rinsed with 3 mL of medium and washed off into new Eppendorf tubes with 600 μL of boiling buffer (100 mM Tris-HCl, 4 mM EDTA, pH 7.5, autoclaved and supplemented with phosphatase inhibitor cocktail 2 (Sigma-Aldrich, P5726)) ...
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bioRxiv - Microbiology 2019Quote: NA activities of recombinant AIV strains were measured using the fluorogenic substrate MUNANA (2’-[4-Methylumbelliferyl]-alpha-D-N-acetylneuraminic acid; Biosynth and Sigma-Aldrich), as described previously [70] ...
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bioRxiv - Developmental Biology 2020Quote: ... After 4 days, cells were switched to DMEM/F-12, HEPES (31330038, Thermofischer Scientific) with 2% Horse serum (H1270, Sigma-Aldrich). Before staining ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with 4% formaldehyde for 20 min and stained with 1x crystal violet solution (2% crystal violet (Sigma-Aldrich), 20% ethanol ...
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bioRxiv - Cancer Biology 2021Quote: ... targeting EZH2 (shEZH2#3 [TRCN0000040076] and shEZH2#4 [TRCN0000010475]) and FAK (shFAK#2 [TRCN0000121211], shFAK#3 [TRCN0000121318], and shFAK#21 [TRCN0000121321]) were purchased from Sigma-Aldrich. pRK5-TGFβRI-FLAG plasmid (#14833 ...
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bioRxiv - Bioengineering 2021Quote: BMOs were collected from the array as described above and fixed with 2 % or 4 % PFA or 10 % Formalin (Sigma, HT501128) overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed with 4% formaldehyde solution diluted in PBS during 2 hours and stained with 1% Naphtol blue black (Sigma, USA) solution for 1 hour for plaque visualization ...
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bioRxiv - Developmental Biology 2020Quote: ... They were then rinsed 4 times with the same medium free of GFP-nanobodies and fixed at room temperature for 2 hours with PBS supplemented with 4% paraformaldehyde (PFA) and 1% BSA (A7638 Sigma-Aldrich). Open-books were observed with a STED microscope (TCS SP8 ...
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bioRxiv - Cell Biology 2021Quote: ... central back skin of K14ΔNβ-cat x PDGFRαH2BEGFP transgenics was clipped and treated topically with 100 µl 4-Hydroxytamoxifen (4OHT) (2 mg/ml dissolved in acetone; Sigma-Aldrich) every second or third day for a total of 6 applications before and after sham or acUVB exposure (see experimental design in Figure 5C) ...
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bioRxiv - Genomics 2022Quote: ... bead/chromatin complexes were washed twice with Low Salt Wash Buffer at 4°C (Final 20 mM Tris-HCl, pH 8,0; 2 mM EDTA; 150 mM NaCl (Sigma-Aldrich, S6546); 1% Triton-X ...
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... DOI (1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane hydrochloride-hydrochloride) and serotonin were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). LP44 (4-[2-(methylthio)phenyl]-N-(1,2,3,4-tetrahydro-1-naphthalenyl)-1-piperazinehexanamide hydrochloride ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Microbiology 2022Quote: ... 100 μl of 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT, 0.5 mg/ml in PBS, Sigma-Aldrich, USA) with 1 μM of menadione (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... 34 mg/mL chloramphenicol) and 2 μL DMSO containing 100x desired final concentration of 5-aminoimidazole-4-carboxyamide-ribonucleoside (Z) (Millipore Sigma) in 2 mL 96-well culture blocks ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride) and lysed by the addition of 10 mg hen egg white lysozyme (Sigma, L6876) and sodium deoxycholate (Acros Organics ...
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bioRxiv - Neuroscience 2024Quote: ... they were cryopreserved for 48h at 4°C in a 30% sucrose solution and subsequently frozen with 2-methylbutane solution (Sigma-Aldrich). Parallel free-floating coronal sections (30-µm-thick ...
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bioRxiv - Molecular Biology 2024Quote: ... The transfection plasmids were mixed in a 4:2:1 ratio in Opti-MEM medium utilizing the polycationic agent polyethylenimine (PEI; Sigma-Aldrich). Following transfection ...
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bioRxiv - Neuroscience 2024Quote: ... The coverslips were then washed again for 2 min with the same 4% poly-acrylamide (PA) gel solution supplemented with the polymerizing agents ammonium persulfate (Sigma, A3678) and TEMED (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... diluted in DMEM without FBS and red phenol supplemented with 2 mM L-glutamine and 25 µM verapamil (V4629, CAS 152-11-4, Sigma Aldrich) for 1 h at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... U-937 cells were maintained in RPMI-1640 medium supplemented with 4.5 g/L glucose, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1.0 mM sodium pyruvate (Sigma-Aldrich; Merck KGaA), 10% fetal bovine serum (Hyclone ...
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bioRxiv - Microbiology 2023Quote: ... Washed beads were adjusted to pH 7.5 with 200 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and bound proteins were reduced using 5 mM dithiothreitol (Sigma-Aldrich) at 37°C for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... was changed daily and animals over 4 days post fertilisation were euthanized by overdose of MS-222 (300 mg/l) or 15% 2-Phenoyethanol (77699 SIGMA-ALDRICH) at the end of experiments.