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Citations for Millipore Sigma :
2101 - 2150 of 10000+ citations for 6 Amino 2 4 difluorobenzotrifluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... adipocytes were lysed in polysome lysis buffer (20 mM HEPES-KOH pH 7.4, 100 mM KCl, 5 mM MgCl2, 1% Triton X-100, 2 mM DTT, 100 μg ml-1 cycloheximide [Sigma] ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was concentrated using ultracentrifugation in an SW-28 rotor (25,000 rpm, 2 h at 4°C) on a 60% OptiPrep density cushion (Sigma-Aldrich). The pelleted viruses were resuspended in 10 mL TNE buffer (50 mM Tris-HCl ...
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bioRxiv - Bioengineering 2023Quote: ... sulfo-succinimidyl-4-(N-maleimidomethyl)cyclohexane-l-carboxylate (sulfo-SMCC, Sigma, at a concentration of 2 mg/mL in PBS) were added to the emulsion and kept to react for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Subcultivation was performed every 2-4 days at a confluence of maximum 80 % using trypsin (Sigma-Aldrich, cat.no. 59427C-100ML). HEK293T-LentiX (Takara Bio ...
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bioRxiv - Immunology 2023Quote: ... cells were centrifuged with 293T retroviral supernatant at 1000×g for 2 hr at 37°C in the presence of 4 µg/mL polybrene (Sigma). Following spin infection ...
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bioRxiv - Cell Biology 2023Quote: ... except the protein sample was concentrated to 4 mg/ml and mixed with 0.005% 3-([3-Cholamidopropyl]dimethylammonio)-2-shydroxy-1-propanesulfonate (CHAPSO; Sigma Aldrich) immediately before vitrification ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were treated with 2% (74.4 mOsmol/kg) or 4% PEG-300 (148.8 mOsmol/kg; v/v; Millipore Sigma 8074845000) in culture medium for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then washed 3 times for 20 - 40 minutes each wash in PBS before incubation with secondary antibody(s) in blocking solution for 2 - 4 hours at room temperature (Alexa 647-conjugated donkey anti-chicken, AP194SA6, Millipore – to label GFP ...
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bioRxiv - Biochemistry 2024Quote: ... Lysate was clarified by centrifugation and incubated for 2 hours at 4 °C with anti-FLAG M2 agarose beads (Sigma) with gentle rotation ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... flies were homogenized in 50 μl PBS per fly (between 2-4 flies per sample) containing 0.1% Tween-20 (Sigma #1379) using a TissueLyser LT (Qiagen ...
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bioRxiv - Bioengineering 2024Quote: ... and polyIC incubation was evaluated using the MTT (3-(4, 5dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). The cells were seeded into 96 well-format cell culture plates at a seeding density of 8ξ103 cells per well ...
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bioRxiv - Immunology 2024Quote: ... Next, 50 μl of 2× STOP buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA (cat. E3889, Sigma-Aldrich) and 100 μg/ml RNase A (cat ...
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bioRxiv - Molecular Biology 2024Quote: MM-NLP particles isolated from both sucrose gradient sedimentation (native) and GraFix (crosslinked) were concentrated to 2 µM using Amicon Ultra-4 centrifugal filters (Ultracel 30K, Millipore). C-Flat 1.2/1.3 (Cu ...
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bioRxiv - Cell Biology 2019Quote: ... the slides with attached intact axonemes were treated overnight at 4°C with blocking buffer containing the diluted primary antibody (mouse anti-acetylated tubulin, Sigma-Aldrich, clone 6-11B-1, 1:1000). On the next day ...
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bioRxiv - Cell Biology 2021Quote: ... 150 μM 4-(4-Morpholinobutylthio)phenol (MoTP) (Sigma) was added to embryos kept at 28.5°C from 24hpf onwards ...
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bioRxiv - Biochemistry 2020Quote: ... and 4×10^-4 1-Thioglycerol (Sigma Aldrich), and incubated for 72 hours ...
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bioRxiv - Neuroscience 2020Quote: ... 4-AP (4-Aminopyridine, 500 μM, Sigma-Aldrich), FK-506 (1 μM ...
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bioRxiv - Bioengineering 2019Quote: ... (ii) 4 hours of 4% sodium deoxycholate (Sigma) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... 4-Hydroxytamoxifen (4-OHT) was purchased from Sigma and dissolved in ethanol ...
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bioRxiv - Developmental Biology 2021Quote: ... 4-hydroxy tamoxifen (4-OHT, Sigma-Aldrich, H6278) treatment (1 µM in EtOH ...
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bioRxiv - Immunology 2021Quote: 4-hydroxytamoxifen (Tx) (4-OHT, #T5648, Sigma-Aldrich) was dissolved in sunflower oil to a concentration of 10 mg/mL for i.p ...
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bioRxiv - Neuroscience 2022Quote: (Z)-4-Hydroxytamoxifen (4-OHT; Sigma-Aldrich #H7904) was first prepared as 25 mg/mL stock solutions in ethanol and frozen at −20C ...
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bioRxiv - Neuroscience 2024Quote: ... and 4-aminopyridine (4-AP, 100 μM, Sigma), mecamylamine (10 μM ...
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bioRxiv - Neuroscience 2022Quote: ... Chemicals used: 4-Aminopyridine (4-AP, Sigma #A78403), tetrodotoxin (TTX ...
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bioRxiv - Developmental Biology 2023Quote: ... 4-hydroxytamoxifen (4-OHT, Cat#: H7904, Sigma-Aldrich) was dissolved at 10mg/ml in peanut oil and 1mg was administered by intraperitoneal injection from E10.5 for 4 consecutive days ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-hydroxytamoxifen (4-OHT; 1 μM; #H7904 Sigma) or vehicle was added to the cell culture medium to activate Cre-LoxP recombination ...
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bioRxiv - Biochemistry 2023Quote: ... 4 mM MgCl2 and 4% DMSO (Sigma, 276855). The mixture was incubated for 30 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 3-ethoxy-4-hydroxybenzaldehyde (121-32-4, Sigma), N-(acid-PEG10)-N-bis(PEG10-azide ...
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bioRxiv - Cell Biology 2024Quote: ... 4-hydroxytamoxifen (4-OH TAM, Sigma-Aldrich, H7904), L-selenocystine (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... the target cells were trypsinized and replated in 6-cm dishes in media containing 2 μg/mL Puromycin (P8833, Sigma-Aldrich Quimica S.A., Madrid, Spain). Selection media was changed every 48-72 h for a minimum of 1 week before cell lines were frozen in 50% FBS and 10% DMSO and stored in liquid nitrogen for later use.
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bioRxiv - Cell Biology 2020Quote: ... were cultured as above in medium supplemented with 1x MEM non-essential amino acids (Sigma). Control young female human fibroblasts (10156 ...
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bioRxiv - Genetics 2021Quote: ... 0.2% (w/v) glucose (Wako) and 0.2%(v/v) MEM amino acid (50x) solution (Sigma). When necessary ...
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bioRxiv - Bioengineering 2020Quote: ... Embryos were anesthetized with tricaine (3-amino benzoic acidethylester, 0.2 mg/mL, Sigma Aldrich, MO), embedded in 1% low-melting-point agarose ...
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bioRxiv - Biochemistry 2020Quote: The nuclear isoform of Pif1 (amino acids 40-859) was cloned into the pET28a (Novagen) expression vector to add an amino-terminal 6XHis tag ...
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bioRxiv - Biophysics 2021Quote: ... The C2A domain (amino acids 1-129) was cloned into the pET-28a(+) vector (Novagen) between the BamHI and HindIII restriction sites ...
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bioRxiv - Systems Biology 2022Quote: ... Proteinogenic amino acids were analyzed after cell growth in M9 containing 15NH4Cl (Sigma-Aldrich, Germany). Cells (1 mL of OD600 1 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in SC media containing 15 mM 3-amino-1,2,4-triazole (3AT; Sigma) and 5 µg/ml α-factor (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... except that embryos were incubated for 1h in pre-equilibrated KSOM (without amino acids; Millipore), prior to incubation in KSOM containing 500 μm HPG or 1 mM EU for 2h ...
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bioRxiv - Microbiology 2021Quote: ... Yeast Nitrogen Base without amino acids and without ammonium sulfate (YNB, Sigma, 1.7 g/l), calcium chloride (0.2 g/l) ...
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bioRxiv - Microbiology 2020Quote: ... 10 μM thiamine and MEM essential and non-essential amino acids (Sigma M5550 and M7145) termed rich defined MOPS (RD-MOPS ...
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bioRxiv - Microbiology 2020Quote: ... RPMI medium 1640 lacking all amino acids (Life Research) was supplemented with isoleucine (Sigma-Aldrich) at a final concentration of 147.5 µM to make Iso medium [44] ...
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bioRxiv - Synthetic Biology 2020Quote: ... Amino acid retention times were verified by a standard mixture (AAS18 Analytical standard; Sigma Aldrich) or dilutions of pure amino acids in 10 mM HCl (for Asn ...
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bioRxiv - Cell Biology 2019Quote: ... a DNA fragment encoding amino acids 91–300 was cloned into the pET30a vector (Novagen) for expression in Escherichia coli ...
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bioRxiv - Physiology 2019Quote: ... OR1D2 antibody (antibody epitope: 201-250 amino acids, Sigma-Aldrich, SAB4502050, St. Louis, MO, USA), rabbit polyclonal antibody against Golf subunit (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2021Quote: ... 1% MEM non-essential amino acid solution and 1% HEPES solution (Sigma-Aldrich, Taufkirchen, Germany). All cells were cultivated at 37 °C and 5 % CO2.
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bioRxiv - Immunology 2021Quote: ... The plates were developed with commercially available 3-Amino-9-ethylcarbazole (AEC) substrate (Sigma-Aldrich). The observed spots were counted using an ELISPOT plate reader by ZellNet and the final data was reported as spot forming cells (SFC ...
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bioRxiv - Immunology 2024Quote: ... The complete culture medium was RPMI-1640 (Hyclone) supplemented with non-essential amino acids (Sigma), 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... 1% minimum essential medium/non-essential amino acid (NEAA, Sigma-Aldrich, St Louis, MO, USA), 7 nl ml−1 β-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 0.19% w/v Yeast Nitrogen Base without ammonium sulphate and amino acids (Sigma-Aldrich Y1251), 2% w/v D-Glucose ...
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bioRxiv - Synthetic Biology 2023Quote: ... 0.19% w/v Yeast Nitrogen Base without ammonium sulphate and amino acids (Sigma-Aldrich Y1251), 2% w/v D-Glucose ...