Labshake search
Citations for Millipore Sigma :
2201 - 2250 of 4429 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: ... qRT-PCR was performed using the KAPA SYBR Fast qPCR Kit (KK4621, Sigma-Aldrich, USA) and universal reverse primer (Table S2) ...
-
bioRxiv - Biochemistry 2023Quote: ... All PCR reactions were conducted using KAPA HiFi Hotstart DNA polymerase (Sigma-Aldrich, catalog #BK1000) unless stated otherwise ...
-
bioRxiv - Genetics 2023Quote: ... The PCR product was cloned into the expression vector pET-30a (Novagen, Madison, WI, USA). The fusion peptide expression and purification were carried out as described previously (Miao et al. ...
-
bioRxiv - Developmental Biology 2023Quote: ... All hPSC lines tested negative for mycoplasma contamination (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich). In summary ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were validated as mycoplasma free by a PCR-based mycoplasma detection kit (Sigma Aldrich).
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... the ChIP-PCR assay was performed using the Chromatin Immunoprecipitation (ChIP) Assay Kit (Millipore, 3753379). The genomic DNA crossed with Foxo1a protein was immunoprecipitated by using 5 μg Anti-6×His tag antibody (abcam ...
-
bioRxiv - Physiology 2023Quote: ... and PCR was performed using KOD Xtreme™ HotStart DNA Polymerase Kit (71975-3, Novagen) following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... The PCR product was cloned in the BglII/BamHI sites of pFLAG-CMV2 (Sigma-Aldrich). The A1625G variant was introduced by site-directed mutagenesis using primers ...
-
bioRxiv - Developmental Biology 2024Quote: ... All hPSC lines tested negative for mycoplasma contamination (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich). All transgenic and KO hESC lines in this study used H9 as the parental line ...
-
bioRxiv - Genomics 2023Quote: ... cells were tested for mycoplasma contamination with LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich #D9307).
-
bioRxiv - Genetics 2023Quote: ... mm9) amplified by the PCR DIG Probe Synthesis Kit (Cat #11636090910, Sigma-Aldrich, MO, USA). The hybridized probe was immunodetected with antidigoxigenin Fab fragments conjugated to alkaline phosphatase (Cat # 11093274910 ...
-
bioRxiv - Microbiology 2020Quote: ... plates supplemented with 3% sheep blood (iTek StPaul MN) and 20 μg/mL neomycin (Sigma St Louis MO) and then grown overnight at 37°C in a 5% CO2 humidified incubator ...
-
bioRxiv - Biophysics 2021Quote: ... Samples were transferred to round bottom 96-well plates and analysed on a Guava® easyCyteTM 8HT (Millipore) flow cytometer ...
-
bioRxiv - Cell Biology 2019Quote: ... Neurons were plated (12-well plate) on poly-L-lysin-hydrochloride (Sigma-Aldrich, St. Louis, Missouri, United States) coated coverslips in plating medium (500 mL MEM ...
-
bioRxiv - Immunology 2021Quote: ... were added to either tissue culture-treated plates coated with 10 μg/ml human plasma fibronectin (Sigma-Aldrich) or Corning® Costar® Ultra-Low Attachment plates (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2021Quote: ... We loaded 2μl of each standard and 20μl of each sample onto a silica gel glass plate (Millipore). Plates were then placed into a chamber pre-loaded with solvent (hexane (4 ...
-
bioRxiv - Microbiology 2022Quote: ... HUVECs were seeded onto tissue culture plates or glass coverslips coated with 0.1 % (w/v) porcine gelatin (Sigma) in 1x PBS ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were plated at 200’000 cells / well in a 24-well plate in 0.5 ml IMDE (Sigma #I3390) containing 10 % fetal bovine serum and 4 mM L-glutamine (Sigma #G7513) ...
-
bioRxiv - Neuroscience 2021Quote: Hippocampal neurons isolated from E16 mouse embryos were cultured on 24-well plates coated with fibronectin (Sigma-Aldrich). The cells were infected with lentivirus-E-SARE-Venus-AkaLuc-PEST on DIV5 ...
-
bioRxiv - Molecular Biology 2021Quote: ... plates were incubated with a 1:20,000 dilution of anti-FLAG M2 HRP-conjugated monoclonal antibody (Sigma-Aldrich) in blocking buffer for another 1 h at RT ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cultures were grown in 6-well plates containing 40 sterile 5 mm glass beads per well (Sigma, 18406). Two replicates were set up ...
-
bioRxiv - Immunology 2021Quote: ... Plates were washed and incubated at room temperature for 30 minutes with 1μg/ml Streptavidin-Alkaline Phosphatase (Sigma). Plates were washed and developed with p-NitrophenylPhosphate (Sigma) ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were scraped off the culture plates and lysed in RIPA buffer supplemented with protease inhibitor cocktails (Millipore) and were then diluted in 4X NuPAGE LDS sample buffer (Life Technologies ...
-
bioRxiv - Genetics 2019Quote: ... we prepared special NGM plates each containing 330 μl of 150 mM FUDR (Sigma Life Sciences, Darmstadt, Germany). These plates were seeded with OP50-1 UV inactivated prior to plating worms to minimize contamination ...
-
bioRxiv - Physiology 2019Quote: ... Citrate synthase activity was measured on a 96-well plate using a commercially available kit (Sigma Aldrich, CS0720).
-
bioRxiv - Bioengineering 2019Quote: ... tissue culture polystyrene culture plates were pre-coated with 20 mg/ml anti-IgG (human, Fc-specific, Sigma) for 1 hour at 37°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... The plates were washed 3 times with pre-cooled PBS prior to the addition of L-DOPA (Sigma). After incubation in L-DOPA for 1 day ...
-
bioRxiv - Immunology 2020Quote: ... coated plates were washed one time with 100 μl/well PBS with 0.05% Tween 20 (PBST) (Sigma-Aldrich), blocked with 100 μl/well of 1% human serum albumin (HAS ...
-
bioRxiv - Neuroscience 2019Quote: ... L2 or early L3 larvae were transferred to molasses plates containing 25 mg/mL RU486 (mifepristone; Sigma Aldrich) and topped with a yeast paste made from 1 g dried yeast and 2 mL 50 mg/mL RU486 in water ...
-
bioRxiv - Synthetic Biology 2019Quote: MDA-MB-231 cells were cultured to 80% confluency on coverslips in 6-well plates and stained with 200µL complete DMEM containing 1µL DiO at a concentration of 1 mM (Sigma) to visualise the cell membrane ...
-
bioRxiv - Microbiology 2019Quote: SH-SY5Y cells were seeded into 24-well plates treated with poly-D-lysine (1 mg/mL; Sigma). When cells reached 90% confluence ...
-
bioRxiv - Microbiology 2019Quote: ... 20µL of this bacterial suspension was then streaked on GHYBHI plates (brain-heart infusion medium supplemented with 0.5% yeast extract, 1.5% agar, cellobiose (1 mg/ml [Sigma]), maltose (1 mg/ml [Sigma]) ...
-
bioRxiv - Plant Biology 2019Quote: ... at least four independent colonies were used for yeast two-hybrid assay on SD-Leu-Trp-His plates with and without 3-amino-1,2,4-triazole (Sigma). Colonies were imaged every 24 h post plating for 4-5 days.
-
bioRxiv - Biochemistry 2020Quote: ... After a final wash the plate was developed by addition of 100 µL tetramethylbenzidine (TMB) (#T5569, Sigma-Aldrich). Assay development was stopped by addition of 50µL 1M sulfuric acid and the assay absorbance measured at 450 nm on a Biotek Synergy 2 plate reader ...
-
bioRxiv - Cancer Biology 2021Quote: ... 24-well plate with glass bottom was coated with 0.1 mg/ml PDL (P0899, Sigma, St. Louis, MO) and 20 ug/ml laminin (L2020 ...
-
bioRxiv - Immunology 2021Quote: ... 2 μl of each reaction were spotted onto a PEI Celluose F thin-layer chromatography plate (EMD Millipore) and run in 1.5 M KH2PO4 ...
-
bioRxiv - Bioengineering 2021Quote: ... The liquid handling platform was programmed to pipette the samples onto a 0.2 μm filter plate (Millipore MSGVN2210) for filtration ...
-
bioRxiv - Cell Biology 2021Quote: Flow cytometry was performed in 96-well plates using a Guava EasyCyte 8HT flow cytometry system (Millipore, USA) equipped with a 488 nm laser and a Cytoflex S equipped with a 405 ...
-
bioRxiv - Cell Biology 2021Quote: ... and/or with plasmid DNA (e.g. 1µg/well for 6-well plates) for 24 hrs using GeneJuice (Merck Millipore). siRNAs targeting USP7 ...
-
bioRxiv - Plant Biology 2021Quote: ... The stress assays were performed on CM plates (Holliday 1974) supplemented with 750 µM calcufluor white (Sigma-Aldrich), 3 mM hydrogen peroxide (H2O2) ...
-
bioRxiv - Plant Biology 2021Quote: ... Arabidopsis seedlings were grown on half-strength MS agar plates supplemented with synthetic hormones GA4 (Sigma-Aldrich, G7276), brassinolide (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2021Quote: ... were transformed with 3 μl of the Gibson mix and plated on four LB plates containing ampicillin (Sigma). We determined that the Gibson cloning efficiency with pTriggerAdaptor was ∼1/250 ...
-
bioRxiv - Systems Biology 2021Quote: hCMEC/D3 cells were grown on 6-well plates coated with calf-skin collagen (Sigma-Aldrich, Gillingham, UK), and collected in TRIzol (Thermo-Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: Coat a 96-well cell-culture plate with 25 uL poly-L-lysine per well (Millipore Sigma, P4707) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2021Quote: ... Filtered cells were immediately seeded in 96 well plates coated with 1% Poly (2-hydroxyethyl methacrylate; Sigma Aldrich) at a density of 200 or 50 cells per well ...
-
bioRxiv - Cancer Biology 2020Quote: ... 105 cells were seeded in a 6-well plate and infected in presence of 8μg/ml polybrene (Sigma). Selection was done with 3μg/ml puromycin (Sigma ...
-
bioRxiv - Cell Biology 2020Quote: All tissue-culture plates or chamberslides were treated with extra-cellular matrix (ECM gel, Cat nb. E1270, Sigma) and chamberslides were in additions pre-treated with Poly-D-Lysine (Cat nb A-003-E ...
-
bioRxiv - Cancer Biology 2020Quote: An optimized number of cells (3 × 103 to 10 × 103) were seeded in 24-well plates (Sigma-Aldrich) and allowed to attach ...
-
bioRxiv - Biochemistry 2022Quote: ... Then 1 µL of the final volume was spotted on a silica gel TLC plate (Supelco Sigma-Aldrich). The plate was placed into a pre-warmed humidified chamber with running buffer composed of 30% H2O ...
-
bioRxiv - Biochemistry 2022Quote: ... Colo205 cells were densely plated on 96-well plates precoated with 0.1μg/mL rat-tail collagen (Sigma-Aldrich) overnight and treated with activating concentrations of Fabs for 5 hours ...