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Citations for Millipore Sigma :
2151 - 2200 of 10000+ citations for TROP 2 Human 187a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 2 mM HEPES (Sigma), 14.5 mM NaOH (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... with 2-Mercaptoethanol (Sigma) and incubated for 5 min at 98 °C ...
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bioRxiv - Immunology 2022Quote: ... 2% Igepal (Sigma-Aldrich), and RNase-free water ...
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bioRxiv - Immunology 2022Quote: ... 2% Tween80 (Sigma #8170611000) in purified water ...
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bioRxiv - Neuroscience 2022Quote: ... SB431542 (2 μM) (Sigma), CHIR99021 (1 μM ...
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bioRxiv - Neuroscience 2022Quote: ... SB431542 (2 μM) (Sigma), CHIR99021 (3 μM ...
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bioRxiv - Immunology 2023Quote: ... coli Rosetta 2 (Novagen). The protein was purified and refolded as previously described (52 ...
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bioRxiv - Immunology 2023Quote: ... 2 ml chloroform (Sigma) were added and mixed throuhgly well by vortexing ...
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bioRxiv - Cell Biology 2023Quote: ... 2 mM cysteamine (Sigma), 50 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM AEBSF (Sigma), 40 µg/mL RNase A ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM ATP (Sigma), 2 mM 2-mercaptoethanol (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... 2% glycerol (Sigma-Aldrich) and 50 µg/ml Gibco hygromycin (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... or 2- butanone (Sigma) diluted in S ...
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bioRxiv - Cell Biology 2023Quote: ... 2-Mercaptoethanol (Sigma-Aldrich) was mixed to samples and proteins were then denaturated at 95°C for 5 min.
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bioRxiv - Cell Biology 2023Quote: ... Alfa Aesar)/2% glutaraldehyde (G7526, SIGMA) in sodium cacodylate buffer (100 mM sodium cacodylate and 2 mM CaCl2 ...
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bioRxiv - Immunology 2022Quote: ... 50mM 2-mercaptoethanol (Sigma) and 1.35mM 2’-deoxygyanosine (dGuo ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 % Glucose (G8270, Sigma), 0.1 % Glox solution [0.5 mg/ml glucose oxidase ...
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bioRxiv - Physiology 2023Quote: ... 2 mM ATP (Sigma), 2 mM β-mercaptoethanol (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... followed by permeabilization and blocking for 1 hour at room temperature in PBS with 0.5% Triton-X-100 (Sigma #X100-500ML)/0.2% Tween-20 (Sigma #P1379)/2% BSA (Sigma #A7906). Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5 ...
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bioRxiv - Physiology 2023Quote: ... coli Rosetta 2 (Novagen), a single colony was inoculated into a 5-ml starter culture and grown overnight before being added to 500 ml Auto-Induction media (Formulation (g/l ...
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bioRxiv - Developmental Biology 2023Quote: ... and 2-mercaptoethanol (Sigma) supplemented with 2iLIF ...
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bioRxiv - Neuroscience 2023Quote: ... and 2% BSA (Sigma) in PBST for 2 h ...
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bioRxiv - Microbiology 2023Quote: ... 2 μM Forskolin (Sigma), 4% EGF (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... 2 mM Glutamine (Sigma), 1% Pen/Step (Sigma) ...
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bioRxiv - Genetics 2023Quote: ... 2 mM Trolox (Sigma)) was added to sample.
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bioRxiv - Molecular Biology 2023Quote: ... 2-mercaptoethanol (SIGMA, M6250), Penicillin-Streptomycin) ...
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bioRxiv - Microbiology 2023Quote: ... aldrithiol (AT-2, Sigma).
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bioRxiv - Microbiology 2023Quote: ... 2 mM CuSO4 (Sigma), and 10 mM ascorbate in TBS buffer in the dark for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... 2 mM MgCl2 (Sigma), 5 mM HEPES (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... 2 mM CaCl2 (Sigma), 2 mM MgCl2 (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... glutamine (2 mM, Sigma), and sodium pyruvate (1 mM ...
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bioRxiv - Neuroscience 2024Quote: ... 1X P/S (Gibco),100μM 2-Mercaptoethanol (Sigma). From day 35 to day 56 ...
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bioRxiv - Cell Biology 2024Quote: ... and 2% glutaraldehyde (Sigma) in 0.1M phosphate buffer pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... 2 % peptone (Sigma #83059) to late exponential/early stationary phase at 30 °C ...
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bioRxiv - Cell Biology 2024Quote: ... 2 µM nocodazole (Sigma) was added 2 h before fixation ...
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bioRxiv - Neuroscience 2024Quote: ... 2 CaCl2 (Sigma-Aldrich), and 4 MgCl2 (Carl Roth ...
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bioRxiv - Microbiology 2024Quote: ... 2′-bipyridyl (Sigma-Aldrich). Mycobactin J was obtained from Allied Monitor (USA) ...
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bioRxiv - Microbiology 2024Quote: ... 2 mM glutamine (Sigma), and 10 μg/mL gentamicin (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich). All assays were performed with cells in passages 4 to 10.
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bioRxiv - Microbiology 2021Quote: ... from strain BetaCoV/Wuhan/WIV04/2019 was ordered from IDT (Coralville, IA, USA) and cloned into a HIS-SUMO expression vector (a modified pET-DUET; Novagen, Madison, WI, USA). After transformation into BL21(DE3) ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
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bioRxiv - Microbiology 2019Quote: ... The washing step was repeated and 200 µl of a 1:5,000 dilution of the monoclonal Anti-His antibody (Sigma-Aldrich, St. Louis, MO) in PBS + 2% BSA was added to each well ...
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bioRxiv - Cell Biology 2019Quote: ... 20mM reduced glutathione) and analyzed by SDS-PAGE followed by Coomassie staining and immunoblotting with anti-His (dilution: 1:5000; Sigma-Aldrich, Steinheim, Germany), or anti-GFP-antibodies (dilution 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... and subjected to SDS-PAGE followed by Coomassie Blue staining and Western Blot analysis using an anti-His antibody (Sigma-Aldrich, Darmstadt, Germany) or anti-MYC antibodies (Hruby et al. ...
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bioRxiv - Systems Biology 2021Quote: ... The N-terminal His-tag was digested at room temperature overnight using up to 12 NIH Units per mL of bovine thrombin (Sigma-Aldrich T4648-10KU). Proteins were then purified using size exclusion chromatography (HiLoad 26/600 Superdex 200 pg column from GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
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bioRxiv - Plant Biology 2022Quote: ... in the same buffer containing 1/5000 of primary antibody raised against poly-His tag directly coupled with peroxidase (Sigma, catalog no. A7058). After washing 3x by PBS 1x ...
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bioRxiv - Microbiology 2022Quote: ... and then transferred to medium differing by the absence of histidine (his) and the presence of 5 mM 3-amino-1,2,4-triazole (3-AT; Sigma-Aldrich, Saint-Louis, MO, USA). Homologous recombination between pPC86 and the PCR product then reconstituted the expression cassette encoding the AD-fused prey protein ...
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bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was sequentially incubated at room temperature for one hour with anti-His and conjugated anti-mouse antibodies (Sigma-Aldrich, Darmstadt, Germany) (Wu et al. ...