Labshake search
Citations for Millipore Sigma :
2401 - 2450 of 10000+ citations for TROP 2 Human 187a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... Recombinant rMSPDHA and rMSPDHB were then expressed through 1.0 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) induction and purified using Ni-NTA His-Bind® Resin Kit (Novagen, San Diego, USA). The purified protein products were quantified by using BCA Protein Assay Kit (Thermo Scientific-Pierce ...
-
bioRxiv - Plant Biology 2019Quote: ... and axr3-3 were expressed as 6X His-tag (N-terminal) fusion proteins in Escherichia coli strain Rosetta™ DE3 competent cells (Supplementary Table 4; Novagen, product code: 70954). These proteins were expressed in minimal media with 13C D-glucose and 15N ammonium chloride ...
-
bioRxiv - Biochemistry 2021Quote: ... His-tagged CLIP-170 fragments and mutants were expressed in BL21 (DE3) and purified by the standard His-tagged purification protocol from Novagen (69670-5, Sigma-Aldrich) with the following modifications ...
-
bioRxiv - Molecular Biology 2019Quote: ... and zinc binding proteins were eluted according to the supplied protocol using HIS-Select wash and elution buffers purchased from Sigma-Aldrich (Saint Louis, MO).
-
bioRxiv - Genomics 2022Quote: ... or T-175 flasks (Hi-C, CUT&RUN) at a density of 1 × 105 cells/mL and treated with 25 nM PMA (Sigma-Aldrich, cat # P1585-1MG). After 24h ...
-
bioRxiv - Biochemistry 2023Quote: Thermal stability of BSA and HSA variants (His-tagged form) and of commercial wild type HSA and BSA (Sigma-Aldrich A1653 and A7638, respectively) was measured by nanoscale differential scanning fluorimetry (nanoDSF) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 3 µl of each stock was then spotted onto the surface of YPD plate (Yeast Extract 1%, Peptone 2%, Dextrose 2%, Agar 2%) without and with 1µg/mL tunicamycin (Sigma-Aldrich Inc., MI, USA). Plates were then incubated for 48-72 hours at 30°C before capturing their photographs ...
-
bioRxiv - Bioengineering 2022Quote: Acetone and Irgacure 2959 (2-hydroxy-1-[4-(2-hydroxyethoxy) phenyl]-2-methyl-1- propanone were obtained from Sigma-Aldrich (St. Louis, MO). 3-([2-(Methacryloyloxy)ethyl]- dimethylammonio)propionate (CBMA ...
-
bioRxiv - Immunology 2024Quote: ... T lymphocytes were adjusted at 50.000 cells/ml in their own culture medium and 0.5 ml of the cell suspension were transferred to 1.5 ml microcentrifuge tubes and incubated at 37°C for 2 hours in the absence or presence of metabolic inhibitors: 50 to 1 mM 2-deoxyglucose (2-DG) (Sigma, catalog: D8375-5G), 200 µM etomoxir (Sigma ...
-
bioRxiv - Cell Biology 2020Quote: Lung fragments (2 mm x 2 mm) were homogenized with TRIZOL (Sigma, Poole, UK) and RNA precipitated by overnight incubation in isopropanol at −20°C ...
-
bioRxiv - Immunology 2021Quote: ... at 2 µg/mL and blocked with 2% bovine serum albumin (A8022, Sigma-Aldrich) dissolved in PBS ...
-
bioRxiv - Physiology 2019Quote: ... 2 μg of EVs were incubated with 2 μM PKH26 dye solution (PKH26GL, Sigma) for 5 mins ...
-
bioRxiv - Neuroscience 2021Quote: ... supplemented with 2% Gem21 NeuroPlex Serum-Free Supplement and 2 mM L-glutamine (Sigma)] ...
-
Intestine-to-neuronal signaling alters risk-taking behaviors in food-deprived Caenorhabditis elegansbioRxiv - Neuroscience 2021Quote: Copper sulfate gradients were visualized using 1-(2-Pyridylazo)-2-naphthol (PAN, Sigma 101036). Plates with 25 μL of 5 mM ...
-
bioRxiv - Neuroscience 2022Quote: ... Imaging buffer containing 2 mM 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox, Sigma), 5mM 3,4-Dihydroxybenzoic acid (DBA ...
-
bioRxiv - Immunology 2020Quote: ... 2 ml of PBS containing 2 mM EDTA and 25 μM cisplatin (Sigma-Aldrich) were added to the cells ...
-
bioRxiv - Biophysics 2021Quote: ... 2 mM 2-mercaptoethanol) supplemented with a protease inhibitor cocktail (cOmplete, Sigma-Aldrich/Merck). Cell extracts were injected into a Ni Sepharose column for affinity chromatography purification followed by size exclusion chromatography of the RnlA-mutant–containing fractions as a final step ...
-
bioRxiv - Biochemistry 2021Quote: ... and 2-Amino-2-(hydroxymethyl)-1,3-propanediol (tris-base) were purchased from Sigma-Aldrich. Hexane ...
-
bioRxiv - Neuroscience 2022Quote: ... Mice were sacrificed under anesthesia with 2-bromo-2-chloro-1,1,1-trifluoro-ethane (Sigma) by cervical dislocation ...
-
bioRxiv - Neuroscience 2022Quote: ... 2 μM FCCP and 2 μM of Rotenone and Antimycin A (all Sigma-Aldrich) were injected after cycle 3 ...
-
bioRxiv - Molecular Biology 2024Quote: ... adults were mounted on 2% agarose pads with 2 μL of 0.1% tetramisole (Sigma) in M9 ...
-
bioRxiv - Bioengineering 2024Quote: ... and 10 μL/mL of the photoinitiator 2-dimethoxy-2-phenylacetophenone (acetophenone; Sigma-Aldrich), prepared by dissolving 300 mg acetophenone in 1 ml of N-vinylpyrrolidone (NVP) ...
-
bioRxiv - Pathology 2023Quote: ... pepsin digestion was performed using 2 mL of 2 mg/mL pepsin (Sigma-Aldrich) solution in 0.01 M HCl for 40 mg dry ECM powder in a 7.5 mL glass vial as previously described [25] ...
-
bioRxiv - Bioengineering 2022Quote: ... nerves were transferred to a 2 ml collagenase solution (2 mg/ml; C9407, Sigma) and incubated for 30 min at 37 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were then eluted with 2 × LDS buffer containing 2.5% 2-mercaptoethanol (M3148, SIGMA).
-
bioRxiv - Physiology 2023Quote: ... 2-aminoethoxydiphenyl borate (2-APB; 100 μM to block SOCE, ECCE, and IP3R; Sigma), or astemizole (1 nM ...
-
bioRxiv - Genetics 2023Quote: ... and rat monoclonal anti-laminin-2 (α2 chain; 4H8-2; Sigma Aldrich; 1:500). Secondary antibodies used were goat polyclonal anti-rabbit Alexa Fluor 594-conjugated (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... Bis(2-hydroxyethyl) terephthalate (BHET) provided by Sigma (Cas: 959-26-2, PN 465151), N-acetyl glucosamine (GlcNAc ...
-
bioRxiv - Biophysics 2023Quote: 2-deoxy-glucose (2-DOG) (Sigma-Aldrich, St. Louis, MO, USA, Cat. No. D3179) was used to block ATP production through the glycolytic pathway and Oligomycin-A (OA ...
-
bioRxiv - Neuroscience 2024Quote: ... and 2 μM antimycin A in combination with 2 μM rotenone (Sigma-Aldrich, 557368). OCR were measured by Seahorse XFe96 Flux Analyzer using a mix (30 s) ...
-
bioRxiv - Genomics 2024Quote: ... 25 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES, Millipore Sigma, H4034-25G), 2.0 mM CaCl2 (Millipore Sigma ...
-
bioRxiv - Molecular Biology 2024Quote: Bioactive lipids t-2-hex and t-2-hex-H2 were purchased from Sigma and applied directly to yeast cultures from 1mg/ml stock solutions in DMSO ...
-
bioRxiv - Microbiology 2020Quote: ... 2 mM L-glutamine (Sigma) and 100 U/ml Penicillin/Streptomycin (Sigma) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 2 % casamino acids (Millipore Sigma), 2 mmol/L MgSO4 (Millipore Sigma) ...
-
bioRxiv - Neuroscience 2021Quote: ... 2-Tribromoethanol (Sigma-Aldrich, #48402) dissolved in Tert-amyl alcohol and sterile 0.9% saline ...
-
bioRxiv - Microbiology 2020Quote: ... 2 M NaCl (Sigma, S5886) and 1xPBS and 0.2 % freshly added ammonium persulfate (APS ...
-
bioRxiv - Immunology 2020Quote: ... 2 μM monensin (Sigma Aldrich) was added for the final 2 hours ...
-
bioRxiv - Cell Biology 2020Quote: ... 2 mM L-glutamine (Sigma), 100 µg/mL Primocin (Invitrogen ...
-
bioRxiv - Cell Biology 2020Quote: ... and 2-deoxyglucose (5mM, Sigma). ROCK inhibitor Y27632 (10uM ...
-
bioRxiv - Cell Biology 2020Quote: ... coli strain Rossetta 2 (Novagen) was used ...
-
bioRxiv - Cell Biology 2020Quote: ... 2 mM NADH (Sigma, N8129) in 0.1 M PK buffer (0.1 M K2HPO4 buffer set at pH 7.2 with NaH2PO4 solution) ...
-
Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: ... + 2% Fish Scale Gelatin (Sigma) + 2% Bovine Serum Albumin (Sigma ...
-
Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: ... + 2% Bovine Serum Albumin (Sigma) + 0.2% Triton X-100 dilute in 1x PBS and probed with 0.5 - 1 μg of primary antibody at room temperature overnight ...
-
bioRxiv - Immunology 2021Quote: ... 2-Mercaptoethanol (50μM; Sigma-Aldrich), Sodium Pyruvate (1mM ...
-
bioRxiv - Developmental Biology 2021Quote: 2-methoxyestradiol (Sigma, m6383-5mg) was resuspended to a 10 mM stock in DMSO ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2-heptanone (Sigma Aldrich, #W254401) test solution was prepared as a 1:10 V/V mix in ethanol absolute ...
-
bioRxiv - Developmental Biology 2021Quote: ... plus 2% chick serum (Sigma) for 5 minutes at 37°C ...
-
bioRxiv - Immunology 2021Quote: 2-DG (1g/kg, Sigma) was injected every other day starting from the day of infection and until completion of the experiment ...
-
bioRxiv - Microbiology 2021Quote: ... 2 mM sodium propionate (Sigma) or 0.02% (wt/vol ...
-
bioRxiv - Immunology 2021Quote: ... 2 μM Oligomycin (Sigma, 75351); Port B ...