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Citations for Millipore Sigma :
2151 - 2200 of 10000+ citations for Recombinant Human SDC2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... pre-coated with 10 μg/ml Human Plasma Fibronectin (Sigma-Aldrich, FC010). Neurons were mechanically shaken off and removed by patting the flask approximately 2 days after inoculation ...
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bioRxiv - Cancer Biology 2024Quote: Human Huh7 cells treated with either DMSO or CHIR99021 (3 µM; Sigma) for 48h were seeded (20,000 cells per well in 100µL ...
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bioRxiv - Neuroscience 2023Quote: ... mouse antibody specific for human SOD1 (SD-G6 1:100, Millipore Sigma), Mouse anti-human HSP70 specific for stress-inducible HSPA1A (SMC-100B 1:100 ...
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bioRxiv - Immunology 2023Quote: ... followed by injection of 6.5 U human chorionic gonadotropin (hCG; Sigma-Aldrich) 48 h later ...
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bioRxiv - Cancer Biology 2023Quote: ... human and bowhead fibroblasts were treated with 50 uM TDRL-505 (Sigma) (diluted 1000x into culture medium from 50mM stock solution in DMSO ...
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bioRxiv - Immunology 2024Quote: ... and (2) the Human Cytokine 23-Plex Discovery Assay® (HD23; Millipore MILLIPLEX® Human Cytokine/Chemokine Magnetic Bead Panel II Immunology Multiplex Assay ...
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bioRxiv - Cell Biology 2024Quote: Human retinal pigment epithelial (RPE) cells were cultured in DMEM (Sigma, #D5648) supplemented with 10% fetal bovine serum and maintained at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... 5 distinct shRNAs directed against CD2AP encoding human CMS (shCMS) (Sigma-Aldrich Mission TRCN shRNA Target set ...
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bioRxiv - Synthetic Biology 2024Quote: ... or 96-well plates pretreated with 20 µg/mL human fibronectin (Millipore) for at least 10 min at 37°C.
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bioRxiv - Synthetic Biology 2024Quote: ... or 24-well plates pretreated with 20 µg/mL human fibronectin (Millipore) for at least 10 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... and then incubated with either (1) 20 µg human fibronectin (EMD Millipore); (2 ...
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bioRxiv - Biochemistry 2024Quote: ... human full length NHE1 was cloned into p3xFLAG-CMV-14 (Sigma-Aldrich) containing a C-terminal 3xFLAG tag ...
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bioRxiv - Plant Biology 2020Quote: ... The protein amount of the resuspended membrane fractions was quantified by Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich) and equilibrated between different samples ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein was measured using the protein extract for PEPCK activity using Bradford reagent (Millipore Sigma; Cat: B6916) and commercial bovine serum albumin standards (Thermo Fisher 23208) ...
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bioRxiv - Developmental Biology 2021Quote: ... Total proteins were extracted from cells with the M-PER Mammalian Protein Extraction Reagent (Sigma, GE28-9412-79) supplemented with protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... Fc chimeric proteins were purified via affinity based Fast Protein Liquid chromatography using Prosep® Ultra resin (Millipore). Fc conjugated proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Microbiology 2019Quote: ... PTP-tagged proteins were detected by incubating with anti-protein A serum (1:3000, 1 h) (Sigma, P3775) followed by Alexa Fluor® 594 goat anti-rabbit (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was measured using Pierce™ BCA Protein Assay Kit (Sigma-Aldrich, St. Louis, Missouri United States) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... solium larval extracts were assessed for protein concentration using a BCA or Bradford protein assay kit (Sigma-Aldrich), respectively.
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bioRxiv - Systems Biology 2021Quote: ... virus protein-bound host proteins were enriched by adding 50 µl anti-HA-agarose slurry (Sigma-Aldrich, A2095) with constant agitation for 3 hours at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 4-HNE protein adducts were detected by an anti 4-HNE protein adduct antibody (1:2000, AB5605, Millipore) in Tris-buffer saline with 5% skim milk ...
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bioRxiv - Microbiology 2020Quote: Oxidized proteins containing carbonyl groups were detected using the OxyBlot Protein Oxidation Detection Kit (Sigma-Aldrich: cat#S7150). Briefly ...
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bioRxiv - Plant Biology 2020Quote: ... Total protein was measured using the protein extract for PEPCK activity using Bradford reagent (Millipore Sigma; Cat: B6916) and a standard curve using commercial bovine serum albumin standards (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: ... The protein solutions were subsequently desalted and protein amount was estimated using Direct Detect cards (Merck Millipore, Darmstadt). All samples were freeze dried before further processing ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg of protein was diluted into 25μL with 5x concentration SYPRO Orange Protein Gel Stain (Sigma Aldrich) in duplicate ...
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bioRxiv - Neuroscience 2024Quote: ... The recovered protein pellet was further processed for western blot-based analysis and quantitation of TH protein (Millipore, Temecula ...
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bioRxiv - Cancer Biology 2023Quote: ... the protein concentrations were measured using a standard BCA assay with Pierce™ BCA Protein Assay Kit (Sigma). 30 μg of proteins was loaded into a pre-cast gel (Novex™ 16% Tricine Gels ...
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bioRxiv - Cell Biology 2023Quote: Protein carbonyl formation by oxidative reactions was assessed with the OxyBlot Protein Oxidation Detection Kit (S7150, Merck Millipore), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin pre-clearing was done by incubating with Protein G Plus/Protein A Agarose beads (Merck Millipore #IP05) for 4 hrs at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Biophysics 2020Quote: Both unlabeled and 15N labeled recombinant full-length αS was expressed and purified in Rosetta™ 2(DE3) pLysS (EMD Millipore®) cells using IMPACT™ system protocol (New England Biolabs® ...
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bioRxiv - Cell Biology 2022Quote: Recombinant His6-EGFP used in the GFP intensity calibration (Figure 1B) was expressed in Rosetta 2 (DE3) competent cells (Millipore Sigma #71400) using pDual-EGFP plasmid (Addgene ...
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bioRxiv - Developmental Biology 2024Quote: ... Serial dilutions from 2μg to 0.0625μg of recombinant SPARC (SRP3159, Sigma-Aldrich; S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems) were spotted and used as standards ...
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bioRxiv - Cell Biology 2023Quote: The custom recombinant antibody-like anti-MAR binding reagent (anti-MAR) was generated and purified in-house (now available from Millipore Sigma, MABE1076) (Gibson et al. ...
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bioRxiv - Biochemistry 2023Quote: We performed the phosphorylation kinetics presented in Figure 4a using commercial recombinant kinases GST-p38βat 10 μg/mL (Sigma-Aldrich, ref. B4437), GST-Erk2 at 20 μg/mL (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: TKO and QKO mESC were cultured in Serum/LIF media (DMEM-KO, 15% FBS, 1000 U/ml murine recombinant LIF (Millipore Sigma #ESG1106), 1× nonessential amino acids (100x ...
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bioRxiv - Microbiology 2023Quote: ... were transformed with the lithium acetate method 45 and strains containing the recombinant plasmids were selected on YPD agar with 200 μg/mL geneticin (G418 – Sigma A1720-5G).
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products from each targeted exon were purified and mixed with 0.5 units of recombinant spCas9-GFP (Sigma-Aldrich, Cat # CAS9GPRO), 10 μM gRNA in 2 μL of 10x Cas9 reaction buffer ...
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bioRxiv - Plant Biology 2020Quote: ... antibodies and Protein A agarose beads (Sigma). After elution of immune complexes ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were blotted onto Immobilon-P (Millipore) with a semi-dry blotting apparatus (TRANS-BLOT SD ...
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bioRxiv - Cancer Biology 2021Quote: ... protein G beads (EMD Millipore, #16-266), biotin (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred to PVDF membrane (Millipore). The membrane was cut into strips corresponding to the molecular weight of proteins of interest ...
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bioRxiv - Cell Biology 2020Quote: ... Polyclonal rabbit-anti-Protein A antibody (Sigma) and monoclonal mouse-anti-TAC102 antibody were used as described above ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with trypsin (Sigma-Aldrich) overnight at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... 60μl of protein-A-Sepharose (P9424, Sigma) was added and samples were incubated 3h at 4°C under agitation ...
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bioRxiv - Cell Biology 2020Quote: Protein was isolated in RIPA buffer (Sigma) and subjected to SDS-PAGE as described previously ...
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bioRxiv - Biophysics 2020Quote: ... proteins were transferred to PVDF membranes (Millipore).
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bioRxiv - Developmental Biology 2021Quote: ... Protein Phosphatase 2A1 bovine (Millipore-Sigma, P6993) was used to digest 100 μl total protein extracted from O9-1 CNCCs ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein Phosphatase 2A1 bovine (Millipore-Sigma, P6993) was used to digest 100 μl total protein extracted from O9-1 CNCCs ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein was detected by enhanced chemiluminescence (Millipore).