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Citations for Millipore Sigma :
2151 - 2200 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... and 5 IU human chorionic gonadotropin (hCG; Millipore) 48 h later ...
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bioRxiv - Immunology 2024Quote: ... 800 ml of human Serum (Sigma Aldrich # H4522) were thawed at 37°C after addition of cOmplete™ ...
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bioRxiv - Neuroscience 2024Quote: ... human citrated plasma (Sigma Cat No. P9523-1ML) was added and time to clot formation was recorded.
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 5% Human serum (Sigma H4522-100ML), 1% Pen/Strep (Thermofisher 15140-122 ...
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bioRxiv - Genomics 2024Quote: ... + 5% Human Serum (Sigma-Aldrich catalogue number H4522) + 1% Penicillin-Streptomycin (Gibco catalogue number 15140122 ...
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bioRxiv - Immunology 2024Quote: ... and 5% human heat-inactivated AB serum (Sigma). The antibodies used for stimulation were anti-human CD3 (clone UCHT1 ...
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bioRxiv - Immunology 2024Quote: ... an added positive human IgG Kappa (Sigma-Aldrich) loaded control lane was included (lane P) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were detected by Western blotting using anti-Protein A from rabbit (for detecting TAP fusion proteins; P3775; Sigma), anti-Gsp1 from rabbit (Makhnevych et al. ...
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bioRxiv - Immunology 2020Quote: ... Protein extracts were then precleared with protein G beads (EZview™ Red Protein G Affinity Gel, Sigma-Aldrich, Israel), following incubation for 30 min at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... were coated with a mixture of biotinylated pentameric FN7-10 (a four-domain segment of fibronectin responsible for cell binding and containing the RGD and PHSRN motifs70) and biotinylated bovine serum albumin (Sigma Aldrich) at a ratio of 1:10 ...
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bioRxiv - Neuroscience 2021Quote: To observe integrity of PNNs and monitor the effect of ChABC or AAV1hSynapsinCre local knockout staining biotinylated Wisteria floribunda agglutinin (WFA, 1:150, Sigma, Germany), and anti-aggrecan primary antibody (1:150 ...
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bioRxiv - Developmental Biology 2020Quote: ... slices were washed three times in PBS for 5min and incubated with biotinylated anti-rabbit IgG (1:500 in PBS, Sigma-Aldrich) at room temperature (RT ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated with 10 μg/ml mono-biotinylated Fab’ goat-anti-IgM+G (αM-PLB) (Liu et al. 2012) or the same molar amount of biotinylated Tf (16 μg/ml, Sigma-Aldrich) (Tf-PLB ...
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bioRxiv - Neuroscience 2020Quote: ... and manual application of 100μl of directly biotinylated mouse monoclonal IgG1 antibodies against Aβ (82E1, IBL, 0.2 μg/ml or 4G8, Millipore, 2 μg/ml) for 8 hours ...
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bioRxiv - Neuroscience 2019Quote: ... tissue was incubated in secondary antibody (Biotinylated horse anti-mouse IgG 1:500, BA-2001, Vector Laboratories, Burlingame, CA; biotinylated donkey anti-goat IgG 1:500, AP180B, Millipore-Sigma ...
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bioRxiv - Plant Biology 2019Quote: ... before being rinsed twice for 15 min each in PBS/BSA and incubated for 15 min in a 1/100 dilution (in PBS/BSA) of biotinylated goat anti-rabbit secondary antibody (Sigma Aldrich). Slides were rinsed as before and incubated with a 1/100 dilution (in PBS/BSA ...
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bioRxiv - Immunology 2020Quote: ... 150 mM NaCl and 0.1% Tween-20 or TBST buffer, 2 h) and incubated (4°C, overnight) with biotinylated BSI-B4 lectin from Bandeiraea (Griffonia) simplicifolia (Sigma-Aldrich, #L3759-1MG ...
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bioRxiv - Immunology 2021Quote: ... pBF2*1501 epitope-specific tetramers were prepared by mixing the biotinylated pBF2*1501 complexes and phycoerythrin (PE)-labeled streptavidin (Sigma Aldrich) at a molar ratio of 4:1 ...
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bioRxiv - Biophysics 2022Quote: GMPCPP-stabilized microtubule seeds polymerized from biotinylated and TAMRA-labeled bovine tubulin were attached to the surface of a microchannel using anti-biotin antibodies (Sigma-Aldrich). Microtubules were assembled using 12 μM unlabeled bovine GTP-tubulin for 10 min at 28 °C in polymerization buffer (80 mM K-PIPES ...
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bioRxiv - Molecular Biology 2022Quote: Biotinylated DNA was generating using a forward primer containing a Biotin TEG group on the 5’ end obtained from Sigma Aldrich: Biotin TEG 5’ ...
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bioRxiv - Biophysics 2022Quote: ... The non-reacted biotin was then removed by passing the biotinylated sample through a PD SpinTrap G-25 column (Sigma-Aldrich) with a molecular weight cutoff of 5000 Da ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μg of biotinylated peptides were bound to 25-50 μl of a 50:50 slurry of streptavidin agarose (Sigma-Aldrich), mixed with recombinant proteins or cell lysates ...
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bioRxiv - Molecular Biology 2024Quote: ... or incubations with species-specific biotinylated antibodies (Table S6; Vector) and then streptavidin–horseradish peroxidase (HRP) antibody (Vector; 1:200) before development with diaminobenzidine (Sigma-Aldrich). Counterstaining was performed using toluidine blue ...
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bioRxiv - Microbiology 2024Quote: ... brain slices were incubated with biotinylated lectin (10μg/ml in PBS 0.1M with 0.3% Triton X-100; Sigma, St. Louis, MO, USA) for 24 hours at 4 °C and subsequently incubated with streptavidin Alexa-488 (Invitrogen Molecular Probes ...
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bioRxiv - Neuroscience 2019Quote: Mouse cortical neurons expressing extracellular HA-tagged Nrxn1α for 4 hr were treated with 0.75 μg/mL brefeldin A (Sigma-Aldrich, Cat #B5936) added to the coverslips in a 12-well plate containing conditioned neuronal culture medium ...
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bioRxiv - Microbiology 2019Quote: ... coli cells with a YFP-tagged genomic copy using a previously developed strain (Taniguchi et al., 2011) and grown under chloramphenicol selection (Sigma Aldrich, USA).
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bioRxiv - Neuroscience 2021Quote: UBQLN2 complexes were isolated from HEK293 cells overexpressing FLAG-tagged wild-type UBQLN2 using anti-FLAG M2 Affinity Gel (Millipore-Sigma, A2220). After washing ...
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bioRxiv - Microbiology 2023Quote: ... Immunoprecipitation of FLAG-tagged host factors in clarified supernatants with anti-FLAG agarose beads (EZview™ Red Anti-FLAG M2 Affinity Gel, Sigma-Aldrich) was performed at 4°C for 45 minutes ...
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bioRxiv - Plant Biology 2024Quote: AM 810–813 plasmids (Table S2) encoding active and proteolytically dead (PD) GST-tagged AtATG4A and AtATG4B were used to transform Rosetta (DE3) cells (Novagen, 70954-3). Expression was induced overnight at 16°C using 0.25 mM IPTG (Hellobio ...
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bioRxiv - Cancer Biology 2021Quote: Anti-Flag (Cat. number: F7425) and anti-His (Cat. number: SAB1306085) antibodies were from Sigma-Aldrich (St. Louis, MS, USA). Anti-phospho-γ-H2AX (Ser139 ...
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bioRxiv - Molecular Biology 2020Quote: Aliquots of 3 μg of GST-LC3B (Viva Bioscience) were incubated alone or with 250 ng of His-STK4 (Millipore) in in vitro kinase reaction buffer (10 mM MgCl2 ...
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bioRxiv - Biochemistry 2020Quote: Full length human KBP residues 1-621 in a PSTCm1 expression vector (with kanamycin resistance and a N-terminal thrombin cleavable 6 x His-tag) was expressed in Rosetta2 cells (Novagen). Following immobilised metal-affinity chromatography with Ni-NTA resin (Qiagen) ...
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bioRxiv - Plant Biology 2019Quote: ... Y187 strains were grown on SD/-Trp-Leu medium and transferred to SD/-Trp-Leu-His medium with 10 mM 3-aminotriazole (3-AT, Sigma) in gradient dilutions (1:10 ...
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bioRxiv - Evolutionary Biology 2020Quote: Proteins were expressed in a pET28/30 vector containing an N-terminal His tag with a TEV protease cleavage site (Millipore). For each protein ...
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bioRxiv - Cell Biology 2020Quote: ... the α-PheRS or α-PheRSCys mutant cDNAs were cloned with His tags at the N-terminal end into the pET-28a plasmid expression vector (Novagen). Wild-type β-PheRS cDNAs were cloned into the pET LIC (2A-T ...
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bioRxiv - Biophysics 2021Quote: The bacteriophage T7 gp16 gene was cloned into the pET-16b vector (ampr and 10x-His tag) from Novagen (Germany), inserted between NdeI and XhoI restriction sites ...
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bioRxiv - Biochemistry 2020Quote: ... Trx-His(6)-hSLAP2 or Trx-His(6)-Δlinker-hSLAP2 were cloned in frame into multiple cloning site (MCS) 1 of pETDuet-1 (Novagen), with CBL (47-357 ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA sequence encoding 1053-1264 aa mouse was amplified and cloned upstream of sequences encoding a polyhistidine stretch (His) tag in pET23b (Novagen). The mGli2-His recombinant protein was expressed in BL21 (DE3 ...
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bioRxiv - Plant Biology 2021Quote: ... The cell lysate was centrifuged for 30 minutes at 5,000 x g and the supernatant was recovered and mixed with HIS-Select Nickel Affinity Gel (Sigma Aldrich) for protein purification (manufacturer’s instructions were followed) ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was centrifuged and the resulting supernatant was run through a column filled with N-NTA His-band resin (Millipore). Bound protein was eluted and then dialyzed for overnight in PBS at 4□ ...
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bioRxiv - Microbiology 2021Quote: ... through immunoblotting using anti-His primary antibody raised in mice and monoclonal (goat) anti-mouse secondary HRP-conjugated antibody (Sigma). Further ...
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bioRxiv - Immunology 2020Quote: ... Membrane was then stained with 1 μg/mL of HRP coupled anti-His (monoclonal mouse anti-polyHistidine-Peroxidase, Sigma-Aldrich) in 1% w/V BSA in PBS-T for 1 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... served as blocking buffer and monoclonal mouse-derived anti-HIS antibody (LS-C57341, LsBio) and HRP conjugated goat anti-mouse IgG antibody (AP308P, Sigma) served as primary and secondary antibodies ...
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bioRxiv - Plant Biology 2020Quote: ... the coding sequence was excised with BamHI/EcoRI and ligated downstream of the His tag into the multiple cloning site MCS1 of pETDuet-1 (ampicillin resistance; Novagen), yielding Sll0171-pETDuet-1 ...
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bioRxiv - Biochemistry 2022Quote: ... the protein sequence fused to an N-terminal GB1 solubility tag and a 6x TEV-cleavable His-tag was cloned into pET24b (Novagen). Plasmids were transformed into E ...
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bioRxiv - Bioengineering 2022Quote: ... It was cloned in the expression vector pET30 Ek/lic containing an N-terminal His-tag with the help of the ligation independent Ek/lic system according to the protocol proposed by Novagen, UK ...
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bioRxiv - Cell Biology 2020Quote: ... bacterial pellet was resuspended in 20 ml of cold His-lysis buffer (50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8.0) with protease inhibitor (Sigma-Aldrich), followed by sonication (10 x 20-second pulses) ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed 4 x 5 min with TBS and the bound SadP was detected with 1:10000 dilution of anti-His primary antibody (Sigma) and 1:20000 dilution of HRP-labelled secondary rabbit anti-mouse antibody (DakoCytomation) ...
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bioRxiv - Immunology 2021Quote: ... Germany) were precoated with murine anti-HIS mAb at a dilution of 1:2,500 (v/v; Sigma-Aldrich, Munich, Germany) at 4°C overnight ...
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bioRxiv - Microbiology 2019Quote: ... This was followed by four washes with 200 µl of incubation buffer and incubation with 50 µl of anti-His-HRP antibody (Sigma), diluted 1:2000 in incubation buffer for 1 hr at 24°C ...