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Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Protein G (Sigma) was coupled at 10 µg/mL in the presence of 10 mM sodium acetate buffer pH 4.5 at 30 µL/min for 300 seconds until ∼3500-4000 RU was immobilized ...
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bioRxiv - Microbiology 2021Quote: ... Protein G (Sigma) was covalently coupled following activation ...
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bioRxiv - Biophysics 2020Quote: ... Protein G (Sigma) at 10 μg/mL was coupled in the presence of 10mM sodium acetate buffer pH 4.5 at 30 μl/min for 300 seconds in various channels ...
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bioRxiv - Molecular Biology 2021Quote: ... elution profiles and sample purity were confirmed by Western blot with antibodies against His tag (Sigma, H1029, lot 033m4785) and Strep tag (IBA ...
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bioRxiv - Biophysics 2022Quote: ... 5 μL of the dye solution was added to 1 mL 5 mg/mL HI (91077C, Sigma-Aldrich, 95%) monomer solution ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 3×106 co-transformants was initially screened for growth on synthetic defined media (SD)-Leu-/ Trp-/ Ura-/ His- media containing 20mM of 3-amino-1,2,4-triazole (3-AT, Sigma-Aldrich). Plasmids were isolated from the potential positive colonies ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting transformants were spotted on SD (−Leu, −Trp) and SD (−Trp, −Leu, −His) selection media containing different concentration of 3-amino-1,2,4-triazole (Sigma, A8056) as previously reported (Putarjunan et al. ...
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bioRxiv - Biochemistry 2021Quote: ... and the lysate was incubated for 1 h with a previously equilibrated His-Select Nickel Affinity Gel (Sigma-Aldrich). Weakly-bound proteins were removed using buffer A (20 mM imidazole ...
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bioRxiv - Neuroscience 2019Quote: ... Adult zebrafish were assayed for response to a mixture of 8 amino acids (Ala, Cys, His, Lys, Met, Phe, Trp, and Val; 0.1 mM each in distilled water; Sigma). No stimulation was provided for the first 5 mins following which the amino acid mix was introduced in one side of the tank at the rate of 1 ml/min using a syringe pump ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting lysate was clarified by centrifugation and the supernatant was applied to Ni2+ -NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and His wash buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... The his-tag affinity purified stator was concentrated to 1 ml using a 100 K MWCO Amicon device (Millipore). The samples were subjected to size exclusion chromatography using Enrich SEC650 column (Bio rad ...
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bioRxiv - Microbiology 2020Quote: ... The culture media for the dividing cells (HI-10) consisted of RPMI-1640 medium + 10% heat inactivated FBS (Sigma), 100 μg/mL penicillin and 100 μg/mL streptomycin ...
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bioRxiv - Plant Biology 2022Quote: ... The transformants were then spotted on SD (−Trp, −Leu, −His) selection media containing 1 mM 3-amino-1,2,4-triazole (Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
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bioRxiv - Immunology 2024Quote: ... whereas CoMiX-FHR4 molecules and VHH controls were detected with a mouse anti-His HRP-conjugated mAb (Sigma-Aldrich). The plate was revealed with OPD/H2O2 HRP-chromogenic mix substrate and the absorbance was measured at 492 nm ...
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatants were harvested and Fab fragment was purified via the heavy chain His tag using affinity chromatography with Talon resin as per the manufacturer’s specifications (Sigma). Purified protein samples were dialysed into PBS as quantified by absorbance at 280nm.
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was filtered through a 0.22 µm filter then applied to a 5 mL His-TrapTM HP (Sigma) column pre-equilibrated with the same buffer at a flow rate of 1.5 mL.min-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... The 8 ml supernatant was applied to 1 ml packed volume of Ni-NTA His-Bind Resin (Millipore Sigma). Binding was performed with gentle mixing for 1 hour at 4°C and loaded onto a Poly Prep Chromatography Column (BioRad) ...
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bioRxiv - Immunology 2023Quote: ... and digested in 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting lysate was clarified by centrifugation and the supernatant was applied to Ni2+-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and His wash buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: His-FUS LC was labeled with Atto-488 or Atto-647 NHS-ester fluorescent dyes (ATTO-TEC, Sigma-Alrich). Labeling occurred at or near the N-terminus because only the N-terminus and a lysine at residue position 5 in the leader sequence preceding the N-terminal region hexa-histidine tag were expected to react with the NHS-functionalized dye ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was isolated and combined with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) His Bind resin (Novagen), incubated at room temperature for 20mins and poured into a 1-cm separation column (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting transformants were spotted on SD (−Leu, −Trp) and SD (−Trp, −Leu, −His) selection media containing different concentrations of 3-amino-1,2,4-triazole (Sigma, A8056) as previously reported 21 ...
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bioRxiv - Plant Biology 2024Quote: ... benthamiana were tandem affinity-purified using a His-Bind resin followed by anti-Flag-M2-agarose beads (Sigma-Aldrich) as described32 with slight modifications ...
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bioRxiv - Molecular Biology 2021Quote: ... PHM005 wild type and recombinant strains were grown in Marine Broth (MB) Difco 2216 (Sigma-Aldrich) or Marine Agar (MA ...
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bioRxiv - Developmental Biology 2019Quote: ... and 1000U/ml ESGRO® recombinant mice leukemia inhibitory factor (LIF, Millipore, ESG1107, stock 107U/ml).
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bioRxiv - Cell Biology 2020Quote: ... Solubilized recombinant FL-CatSper1 and ND-CatSper1 were pulled-down with anti-HA agarose (EMD Millipore) for 1 hr at RT ...
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bioRxiv - Biochemistry 2021Quote: ... Immunoassay plates were coated with 2.5 μg/ml recombinant angiotensin converting enzyme-2 (ACE2, Sigma Aldrich). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... or recombinant leptin (1 mg/kg/d dissolved in dimethyl-sulfoxide) (L 3772 Sigma Chemical Co.), or mdivi-1 (50 mg/kg/d dissolved in dimethyl-sulfoxide ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant PRDX6 was purified by gel filtration chromatography on a Superdex 200 16/600 column (Sigma) equilibrated with 20mM HEPES at pH 7.0 ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant L-1-Tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin was obtained from Sigma-Aldrich. Recombinant cathepsin B and cathepsin L were purchased from R&D Systems.
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bioRxiv - Cell Biology 2021Quote: ... The recombinant hormone was dissolved in sterile Lebovitz medium (L-15) (Sigma-Aldrich, St. Louis, USA) at the concentration of 100 µg/mL ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant L-1-Tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin was obtained from Sigma-Aldrich. Human alpha-1 PDX (alpha1-PDX ...
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bioRxiv - Microbiology 2020Quote: ... hAEC cultures were pretreated with recombinant universal type I IFN (100 or 10 IU/ml; Sigma) or recombinant IFN-λ3 (100 or 10 ng/ml 56 ...
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bioRxiv - Bioengineering 2022Quote: ... Beefy-9 medium was prepared by adding 0.8 mg/mL of recombinant albumin (Sigma #A9731-1G) to Hi-Def B8 ...
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bioRxiv - Molecular Biology 2022Quote: ... The recombinant HNRNPH1 was analyzed using an anti-FLAG antibody (F1804, Sigma-Aldrich, St. Louis, MO) and an anti-mouse HRP secondary antibody (Cell Signaling Technology ...
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bioRxiv - Pathology 2023Quote: ... 10 µL of recombinant Tat-Cre at 8.8 µg/µL supplemented with Fast Green FCF (Sigma) to facilitate visualization of TAT-Cre delivery were intravaginally injected to each uterine horn using a micropipette tip (Figure 4A) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were cultured with recombinant retroviruses or lentiviruses and 10 μg/mL polybrene (H9268; Sigma-Aldrich), and they were selected in growth medium with puromycin (14861-71 ...
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bioRxiv - Immunology 2024Quote: ... 20 ng/mL recombinant mouse GM-CSF (Miltenyi-Biotec) and 0.5 μM ß-estradiol (Sigma-Aldrich) and transduced with ER-HoxB8 retrovirus using Lentiblast Premium (OZ Biosciences).71 After two weeks ...
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bioRxiv - Systems Biology 2021Quote: ... purified LC and naïve CD4+ T cells or TRMs were co-cultured in human serum supplemented media (RPMI, Gibco, UK, 10% human serum, Sigma, UK ...
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bioRxiv - Cancer Biology 2020Quote: Human neutrophils were obtained from fresh human peripheral blood from healthy volunteers and were isolated using a Percoll (Sigma-Aldrich) gradient.
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bioRxiv - Microbiology 2020Quote: ... Membranes were probed with polyclonal goat anti-human ACE2 (1:500, AF933, R&D Biosystems) or rabbit anti-human beat-Actin (1:2500, 6L12, Sigma) followed by donkey anti-goat IRdye 680CW or goat anti-rabbit IRdye 800CW (Abcam) ...
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bioRxiv - Physiology 2020Quote: ... For insulin clearance during an insulin challenge, mice were given human insulin (1 U/kg, NovoRapid) or human C-peptide (50 µg/kg, Sigma) by intraperitoneal injection and blood samples were collected by tail vein sampling at 0 ...
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bioRxiv - Microbiology 2020Quote: ... membranes were probed with 1 in 10,000 dilution of human anti-SIC followed by 1 in 50,000 dilution of HRP-conjugated goat anti-human IgG (Sigma-Aldrich). To directly compare detection of SIC fragments by purified human and rabbit anti-SIC antibodies ...
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bioRxiv - Cell Biology 2022Quote: The human alveolar carcinoma epithelial cell line A549 (ATCC, CCL-185) and human colorectal adenocarcinoma cell line HT29 (Sigma Aldrich) were utilized for toxicological evaluation ...
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bioRxiv - Microbiology 2020Quote: ... 15 µl of secondary antibody (anti human Fab-HRP: Jackson ImmunoResearch, cat# 109-035-097; anti human IgG-HRP: Sigma, cat# A6029-1ML ...
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bioRxiv - Immunology 2022Quote: ... alpha enolase (in-house expressed human) and collagen II (bovine purified, chondrex) and at 0.5 μg/ml hIgG1 to citrullinated fibrinogen (human purifed, Sigma Aldrich).
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bioRxiv - Microbiology 2023Quote: Primary human macrophages (MΦs) were cultured in macrophage medium (RPMI-1640 GlutaMAX™ (Thermo) supplemented with 4% human AB serum (Sigma), 100 μg/ml penicillin-streptomycin (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: Protein carbonylation was determined by OxyBlot Protein Oxidation Detection Kit (Millipore). Wild-type animals were synchronized to the young adult stage ...