Labshake search
Citations for Millipore Sigma :
2151 - 2200 of 10000+ citations for 6 1 Aminoethyl 2H 1 4 benzoxazin 3 4H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2024Quote: ... and the nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI, Sigma‒Aldrich, D9542). Image capture was performed by a laser scanning confocal microscope (Olympus ...
-
bioRxiv - Neuroscience 2024Quote: ... Counterstaining was performed using the fluorescent nuclear dye 4′,6-diamidino-2-phenylindole (DAPI) (Sigma). In situ hybridization was performed on 60 μm vibratome sections using digoxigenin-labelled antisense probe for Rorα ...
-
bioRxiv - Neuroscience 2023Quote: ... all slides were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) (D9542, 1100, Sigma Aldrich). Slides were then rinsed and coverslipped with Prolong Gold antifade reagent ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... Cell nuclei were stained by 4’,6-diamidino-2-phenylindole (DAPI, catalog #6329; Sigma Aldrich). Sections were first washed once in PBS for 10 min ...
-
bioRxiv - Immunology 2023Quote: ... Dead cells were excluded through the use of 4’-6’-diamidine-2′-phenylindole (DAPI) (Sigma). Alive DAPI- CD19+RBD+ B cells were single-cell index sorted using a FACSAria II (BD Biosciences ...
-
bioRxiv - Cancer Biology 2023Quote: ... sections were incubated with 4′,6-diamidino-2-phenylindole dihydrochloride (Sigma-Aldrich, Catalog No. D9542) for 5 min ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... The sections were counterstained with 0.3 μM 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich). For transient inactivation experiment ...
-
bioRxiv - Cancer Biology 2024Quote: ... the nuclei were stained with 4’-6’-diamidino-2-phenylindole (DAPI, Sigma-Aldrich, MO, USA). Stained slides were scanned using a Mantra system (PerkinElmer ...
-
bioRxiv - Neuroscience 2024Quote: ... followed by incubation in 300nM 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich, Catalog# D9542) at room temperature for 10 mins ...
-
bioRxiv - Cell Biology 2020Quote: ... BSA was coupled to the PEG (3000) for 1h at RT with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, 100 mM, Sigma Aldrich, 39391) and N-hydroxy-succinimide (NHS ...
-
bioRxiv - Neuroscience 2019Quote: ... samples were washed 3 × 3 minute with PBS followed by a second 30-minute fixation step in 1% osmium tetroxide (Sigma-Aldrich, 75632). A final 3 × 3 minute PBS wash step was performed before samples were serially dehydrated in ethanol with a final 30-minute dehydration step using hexamethyldisilazane (HMDS ...
-
bioRxiv - Neuroscience 2020Quote: ... to 30 µL CSF (pooled from 1 – 3 animals) and placing in 3 mm NMR tubes (Wilmad®, 7 inch; Sigma-Aldrich). Serum samples (samples pooled correspondingly to the CSF samples ...
-
bioRxiv - Microbiology 2020Quote: ... ACE2 (15 μg/mL) was immobilized on AR2G biosensors activated by 1-ethyl-3-[3-dimethylaminopropy] carbodiimide hydrochloride (EDC, E1769, Sigma, Shanghai, CHN) and sulfo-N-hydroxysulfosuccinimide (s-NHS ...
-
bioRxiv - Cell Biology 2020Quote: ... Then the cells were washed with PBS 3 times and incubated with cyanine 3 (Cy3)-conjugated donkey anti-goat IgG secondary antibody (1:500, Millipore, Temecula, CA) at room temperature for 1 hour ...
-
bioRxiv - Neuroscience 2021Quote: ... Following two 2h washing steps in PTwH (PBS / 0.2% Tween-20 (P9416, Sigma Aldrich) / 0.01mg/ml Heparin ...
-
bioRxiv - Neuroscience 2020Quote: ... Transcription was inhibited by 2h exposure to 8mg/ml of Actinomycin D (Sigma-Aldrich). Histone deacetylases were inhibited by 12h treatment with 200nM Trichotstatin A (Abcam).
-
bioRxiv - Neuroscience 2021Quote: ... pH 7,4) for washing steps and the 2h-long block in 5 % Chemiblocker (Millipore), 0.5 % Triton X-100 in PB ...
-
bioRxiv - Microbiology 2023Quote: ... and incubated for 2h with antibodies recognizing the FLAG-tag (F-1804; Sigma-Aldrich), Strep-tag (NBP2-41073 ...
-
bioRxiv - Molecular Biology 2021Quote: ... which was carried out as two rounds of dialysis against 1 mM DTT in H2O and one round against 0.5 mM TCEP (Sigma) before lyophilisation and storage ...
-
bioRxiv - Evolutionary Biology 2019Quote: One percent (1%) Aqueous Silver Nitrate Solution was made by adding 1g of Silver Nitrate (Sigma Cat 56506-256) to 100 ml of distilled water ...
-
bioRxiv - Biochemistry 2019Quote: ... The sample was incubated for one minute before blotting with filter paper Whatman n° 1 (Sigma Aldrich, Munich, Germany). Subsequently ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were incubated in one of three conditions for 24h: 1) 1μg/mL tetracycline (Bioshop) and 50 μM biotin (Sigma); (2 ...
-
bioRxiv - Plant Biology 2022Quote: ... Seedlings at the one leaf stage were treated in 1/2 Hogland solution (pH 6.0) containing 1.5% NaCl (Sigma-Aldrich) or 20% polyethylene glycol (PEG ...
-
bioRxiv - Biochemistry 2022Quote: ... The mediators used in the potentiometric measurements were methyl viologen (1,1′-Dimethyl-4,4′-bipyridinium dichloride) and pyocyanin (5-Methylphenazin-1-one) also purchased from Sigma-Aldrich. Sodium hydrosulfite and potassium ferricyanide for potentiometric experiments were purchased from VWR.
-
bioRxiv - Physiology 2020Quote: ... or intravenous E.coli LPS (1 μg/kg infused over one hour; O55:B5, Sigma-Aldrich, Castle Hill, NSW, Australia). Once a PaO2/FiO2 ratio <100 mmHg (PEEP ≥5 cmH2O ...
-
bioRxiv - Synthetic Biology 2021Quote: ... media was replaced again with 100µL DMEM + 10% FBS + 1% PS containing 10µM furimazine and one of the following SERCA inhibitors: 10µM cyclopiazonic acid (CPA) (Sigma-Aldrich), 10µM 2,5-di-(tert-butyl)-1,4-hydroquinone (DBHQ ...
-
bioRxiv - Microbiology 2022Quote: ... 1 mM Na2EDTA) were combined with one half volume of glass beads (0.5 mm diameter; Sigma-Aldrich, Madrid, Spain) and vortexed for 3 consecutive cycles of 90 sec interrupted by 30 sec intervals on ice ...
-
bioRxiv - Molecular Biology 2022Quote: ... Sporozoites were incubated for one hour at room temperature with mouse anti-tubulin (1:500, Sigma-Aldrich, Ref T5168) to stain microtubules followed by three washing steps with RPMI ...
-
bioRxiv - Immunology 2023Quote: Lungs were harvested and placed in one mL of PBS containing 0.05% Tween 20 and 1 mg/mL DNase I (Sigma). The tissue was homogenized using C-Tubes from Miltenyi Biotech ...
-
bioRxiv - Genetics 2023Quote: ... Primary antibodies were diluted in blocking buffer and incubated at room temperature for one hour with mild agitation (M2 FLAG 1:2,000 (F1804-5MG; Sigma), tubulin 1:5,000 (ab6046 ...
-
bioRxiv - Genomics 2020Quote: ... The lentivirus containing the pLenti-FNLS-P2A-Puro or pLenti-ABERA-P2A-Puro or desired gRNAs were added to the 0.5 million of HUDEP-2 or HUDEP-2 stable cell line in a one well of the six-well plate along with 6 μg/ml Polybrene (Sigma-Aldrich) and 1% HEPES 1M buffer (Gibco ...
-
bioRxiv - Bioengineering 2020Quote: ... However initial mineralized collagen suspensions were created using one of three potential glycosaminoglycans: chondroitin-6-sulfate (Chondroitin sulfate sodium salt from shark cartilage, Sigma-Aldrich), chondroitin-4-sulfate (Sodium Chondroitin Sulfate A ...
-
bioRxiv - Cell Biology 2021Quote: ... each piece of frozen tissue was placed into one well of a 6-well plate with salt-Tris buffer containing 146 mM NaCl (Cat#S6546-1L, Sigma-Aldrich), 1 mM CaCl2 (Cat#97062-820 ...
-
bioRxiv - Biochemistry 2022Quote: EMSAs for the SAP-domain were performed with DNA such that the single strand or one of the two strands in dsDNA was 5’-labeled with fluorescein (6-FAM, Sigma-Aldrich) (Table 1) ...
-
bioRxiv - Cell Biology 2023Quote: ... 20’000 transduced MEFs were then seeded into one well of a 6 well plate in ‘MES medium’ supplemented with 2ug/ml Doxycycline (D9891-5G, Sigma-Aldrich), 3μM CHIR99021 (4423/50 ...
-
bioRxiv - Microbiology 2020Quote: ... cells were fixed with formaldehyde/PBS solution (6%) and stained with crystal violet (1%; Sigma Chemical Corp.) in 70% methanol ...
-
bioRxiv - Genetics 2021Quote: ... cells underwent negative selection for 6 days with 1 ug/mL doxycycline (to induce promoter expression; Sigma), 100 ug/mL blasticidin S (to kill cells not expressing the blasticidin-resistant plasmid ...
-
bioRxiv - Microbiology 2020Quote: ... cells were fixed with formaldehyde/PBS solution (6%) and stained with crystal violet (1%; Sigma Chemical Corp.) in 70% methanol ...
-
bioRxiv - Developmental Biology 2022Quote: ... The IL-6 inhibitors LMT-28 and the Wnt/β-catenin inhibitor IWR-1 (I0161, SIGMA-ALDRICH) were both used at 10 μM.
-
bioRxiv - Microbiology 2019Quote: ... Cell lysates were incubated 45 min at 37 °C with 0.6 mg.mL−1 fructose-6-phosphate (Sigma) and 0.6 mg.mL−1 glutamine (Sigma) ...
-
bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal anti-Glutamine Synthetase (for Müller glia-specific marker; 1:5000 Millipore #MAB302, clone GS-6); goat polyclonal anti-Brn3a (for retinal ganglion cell marker ...
-
bioRxiv - Cell Biology 2021Quote: ... 6 wells) in 1 ml of media in 24-well plates coated with poly-HEMA (Sigma P3932) to inhibit cell adherence ...
-
bioRxiv - Developmental Biology 2022Quote: ... a mouse monoclonal anti-acetylated tubulin clone 6-11B-1 antibody (IgG2b; product T 6793; Sigma-Aldrich) were used at 1:2,500 dilution and secondary anti-mouse isotype-specific antibody conjugated with Alexa 488 (anti-IgG2b ...
-
bioRxiv - Cancer Biology 2019Quote: ... 6-7-week-old female mice were injected intraperitoneally with 1 mg of Tamoxifen (T5648, Sigma-Aldrich) on three consecutive days ...
-
bioRxiv - Pathology 2020Quote: ... We used primary mouse monoclonal antibodies against anti-α-acetylated tubulin (clone 6-11B-1, Sigma Aldrich and proliferating cell nuclear antigen (PCNA ...
-
bioRxiv - Genetics 2020Quote: ... HUT 78 cells were treated up to 6 hours with 1 μM of romidepsin (Sigma-Aldrich, SML1175), DMSO being used as a control ...
-
bioRxiv - Developmental Biology 2022Quote: ... 1 μg of RNA replicons were transfected into 105 fibroblasts on 6-well plates using RiboJuice (Sigma) in the presence of 100 ng/ml B18R (Promega) ...
-
bioRxiv - Physiology 2024Quote: ... hypothalamic tissue was homogenized in 100% methanol containing 1 mM 6-propyl-2-thiouracil (PTU) (Sigma, H34203) in a glass-glass tissue grind pestle (60mm ...
-
bioRxiv - Genomics 2024Quote: ... Samples were incubated with primary antibodies (mouse anti-acetyl-alpha tubulin antibody, clone 6-11B-1 [Millipore-Sigma Aldrich ...
-
bioRxiv - Developmental Biology 2024Quote: ... followed by 6 PBSTx washes and incubation with anti-DIG antibody (1:1,500; CAT 11207733910; Sigma-Aldrich) overnight at 4°C ...