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Citations for Millipore Sigma :
2101 - 2150 of 10000+ citations for 6 1 Aminoethyl 2H 1 4 benzoxazin 3 4H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... DRGs were centrifuged for 4 min at 500 xg at 4°C and incubated in 1 ml dissociation buffer (2.5mg/ml collagenase, Sigma Aldrich, C9722 ...
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bioRxiv - Cancer Biology 2019Quote: ... The endocrine-resistant cells were kept in phenol-red free medium supplemented with 10% heat-inactivated charcoal-stripped-FBS and 1% P/S with the addition of 100 nM 4-hydroxytamoxifen (4-OHT, H7904, Sigma). All cells were kept at 37°C in a humified incubator with 5% CO2.
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bioRxiv - Microbiology 2020Quote: ... and 0.26g (12.48 x 10−4 mol, 1:4 stoichiometric ratio) cross-linking agent tetraethyl orthosilicate (TEOS – Sigma-Aldrich, 131903) were mixed at 3500rpm for 1 min using a DAC 150FV2-K speedmixer ...
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bioRxiv - Cell Biology 2021Quote: ... Chromatin was pelleted by spinning 5 minutes at 10,000 g at 4°C and subsequently incubated for 1 hour at 4°C in HEPES buffer containing 500 units of Benzonase (Millipore) and 2 µg Pol II antibody (ab5095 ...
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bioRxiv - Biochemistry 2022Quote: ... The IMAC pool was diluted 1:4 in 50 mM Tris HCl pH 8 and applied to 4 mL DEAE-Sepharose (Sigma). After washing with 20 mM Tris HCl ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Canada) and a DNA damaging growth condition supplemented with a UV mimetic molecule50 4-Nitroquinoline 1-oxide (4-NQO) (Sigma–Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... the acrylate functionalized photodegradable monomer was synthesized by suspending 4-[4-(1-hydroxyethyl)-2-methoxy-5-nitrophenoxy]butyric acid (0.0166 mol, Sigma-Aldrich) in anhydrous DCM (90 mL) ...
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bioRxiv - Immunology 2021Quote: ... 3 μg pRSV-Rev and 6 μg of pRRL lentiCRISPR vectors using Poly(ethyleneimine) (PEI; Sigma). Media was replaced 24 hours post-transfection and harvested 24 hours later for filtration with a 0.45 μm filter (SteriFlip ...
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bioRxiv - Immunology 2019Quote: ... the plates were incubated with 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (A3219; Sigma-Aldrich) at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1h and subsequently developed using 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) substrate (Sigma). Absorbance was measured at 405nm in a BioTek Synergy HTX multi-mode reader ...
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bioRxiv - Immunology 2019Quote: ... 3 μg pRSV-Rev and 6 μg of pRRL lentiCRISPR vectors using Poly(ethyleneimine) (PEI; Sigma). Media was replaced 24 hours post-transfection and harvested 24 hours later for filtration with a 0.45 μm filter (SteriFlip ...
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bioRxiv - Immunology 2023Quote: ... 100 μl/well of 2,2’-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt (ABTS, Sigma, A1888) was added to the plates and the reaction was stopped by the addition of 5% SDS ...
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bioRxiv - Microbiology 2024Quote: ... and 100 μg/mL 6-chloro-3-indolyl-β-d-galactoside (Red-Gal) ([Sigma Aldrich], B6149) as indicators ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated overnight at 4°C with anti-BUB-1 (Desai et al., 2003) and anti-tubulin (1/400, clone DM1α, Sigma Aldrich) in AbDil ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-H3S28phos antibodies from Santa Cruz Biotechnology were used at 1:200 dilution in Corning PBS supplemented with 4 mg ml−1 bovine serum albumin (BSA; Sigma-Aldrich). Cell samples were washed and blocked with Corning PBS containing 10 mg ml−1 BSA and 1.5 mg ml−1 casein (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hour before incubation with primary antibodies: rabbit anti-NogoA (1:200; Supplementary Table 4) and mouse anti-GFAP (1:500; Sigma-Aldrich), for 72 hours at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... they were incubated in the washing buffer under agitation (50 rpm) at 4°C for 1 hr with 1 μg/mL of rabbit anti-Flag- (SIGMA, F7425) or mouse anti-SUMO-1 (21C7 from the Developmental Studies Hybridoma Bank ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized cells were clarified for 20 minutes at 21,000 x g at 4°C and incubated for 1 hour with 1 mL bed volume of anti-FLAG M2 affinity gel (Sigma A4596) which had been pre-equilibrated in DBC wash buffer (0.3% DBC ...
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GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... and viral envelope (pMD2G) constructs at a 4:2:1 ratio with 1 mg/mL polyethylenimine (PEI, linear MD 25,000 Da, Sigma, Cat# 408727). The ratio of total transfected DNA to PEI was 1:3 (1 µg DNA:3 µg PEI) ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was done in ATP hydrolysis buffer (1 mM DTT, 4 mM MgCl2, 1 mM PEP, 12 µL LDH/PK enzyme mix (Sigma Aldrich), 50 mM HEPES (pH 7.5) ...
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bioRxiv - Neuroscience 2020Quote: ... in TBST 1X at RT for 1 hour followed by incubation at 4°C overnight with primary antibodies (SYNGAP-1:2K, PA1-046, Thermofisher; b-Actin-1:5K, A2228, Sigma Aldrich). Membranes were washed thrice with TBST (0.1% Tween 20 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in primary antibody diluted in blocking solution (PBST, 1% BSA) overnight at 4°C with the following primary antibodies: mouse anti-NeuN (1:500, Millipore, Mab377), rat anti-BCL11B (1:1,000 ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by overnight incubation with primary antibody overnight at 4 °C (1:100 rabbit-anti-HNF4A Cell signaling 3113S; 1:250 mouse anti-AFP antibody, Sigma A8452 ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 hour and incubated overnight at 4°C with primary antibody (ATRX: 1:500, H-300 Santa Cruz Biotechnology Inc.; INCENP: 1:3000, Sigma). The following day the membrane was washed and incubated in the appropriate horseradish peroxidase-conjugated secondary antibody (1:4000 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were prepared for Western Blot by diluting 8μg protein 1:4 with loading buffer (1:10 β-mercaptoethanol (Sigma-Aldrich) and 9:10 laemmli (Bio-Rad)) ...
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The gut hormone Allatostatin C regulates food intake and metabolic homeostasis under nutrient stressbioRxiv - Physiology 2020Quote: ... supplemented with 1 mL/L propionic acid and 1 g/L methyl-4-hydroxybenzoate) containing 0.5% erioglaucine dye (Sigma-Aldrich, #861146) and allowed to feed for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then incubated with primary antibodies overnight at 4°C: anti-Alpha Tubulin (1:200, DSHB 12G10) and β-catenin (1:10,000 dilution, Sigma ABE208). The membrane was washed three times using PBST ...
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bioRxiv - Neuroscience 2024Quote: ... for 1 h and incubated overnight at 4°C with the specific primary antibody: mouse anti-NeuN 1:200 (Millipore, MAB377), rabbit anti-GFAP 1:1000 (Dako ...
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bioRxiv - Genetics 2024Quote: ... Membranes were incubated overnight at 4°C in primary antibodies (Flag, F7425, Sigma-Aldrich, 1:1000; HDAC2, ab32117 Abcam, 1:1000; acetylated Tubulin, T6793 Sigma, 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with primary antibodies overnight at 4°C (TNRC6A, human index serum, 1:3000; FOXJ1, eBioscience, # 14-9965-82, 1:100; Ac-α-tub, Sigma, # T7451 ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryo and skin sections were then incubated with the primary antibodies overnight at 4°C (LHX2, 1:1000, #3030529 Merck, Darmstadt, Germany; SOX9, 1:1000, #AB5535 EMD Millipore, Burlington ...
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bioRxiv - Bioengineering 2023Quote: ... pH 14), hydrogen chloride solution (c(HCl)=1, pH 1), and buffer solution (pH 4, pH 9) were purchased from Sigma-Aldrich. Methacryloxyethyl thiocarbamoyl rhodamine B (MA-RhB ...
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bioRxiv - Cancer Biology 2021Quote: ... The nuclei were stained by incubation with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) diluted 1:10,000 in 1% BSA for 15min ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were counterstained with 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, cat. D8517, Sigma-Aldrich) diluted 1:3000 in PBS 1X ...
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bioRxiv - Developmental Biology 2021Quote: ... 4% PFA fixed zebrafish embryos were embedded in 6% low-melting agarose (LMA, Sigma-Aldrich) dissolved in 0.1% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... was used to stain filamentous actin and 4′,6-Diamido-2-Phenylindole (DAPI, Sigma-Aldrich) to stain nuclei.
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed as before and stained with 4’,6-diamidino-2-phenylindole (DAPI) (SIGMA) followed by cover-slipping with Fluoro-gel with Tris buffer (Electron Microscopy Sciences).
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated with 4’,6-diamidino-2-phenylindole (D9542-10MG DAPI, Sigma Aldrich, Denmark) to stain DNA followed by 5 times wash in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were counter stained using DAPI (4’, 6-diamidin0-2-phenylidone dihydrochloride, Sigma-Aldrich, USA) and mounted in DABCO (antifading agent ...
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bioRxiv - Microbiology 2022Quote: ... cells were stained with 4',6-Diamidino-2-phenylindole (DAPI) (Sigma, Cat No. 10236276001, Germany) for 10 min ...
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bioRxiv - Developmental Biology 2021Quote: ... were incubated for 6 hours at 4°C with 5 μg H3K27me3 (07-449, Millipore), 5ug H2AK119ub (8240 ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in blocking solution with 0.2 µg/µl 4’,6’-diamidino-2-phenylindole (DAPI; Sigma) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) (0.5 μg/mL, Sigma-Aldrich) and were mounted with ProLong™ Gold antifade reagent ...
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bioRxiv - Immunology 2022Quote: ... Nuclei were stained with 4’-6’-diamidino-2-phenylindole (DAPI; Sigma-Aldrich, St. Louis, MO) after antigen labeling ...
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bioRxiv - Microbiology 2020Quote: ... 4 mM stable glutamine without sodium pyruvate (Biochrom) and supplemented with 6% iFCS (Sigma-Aldrich). Primary human macrophages were cultured in RPMI medium containing 5.5 g × l-1 NaCl ...
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bioRxiv - Immunology 2020Quote: ... Samples were then stained with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich D9542-10MG) at 1μg/mL for 30 sec ...
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bioRxiv - Genomics 2020Quote: ... Preparations were counterstained with 0.5 mg/ml DAPI (4’,6-diamidino-2-phenylindole; Sigma-Aldrich) in DABCO antifade (1,4-diazabicyclo[2.2.2]octane ...
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bioRxiv - Neuroscience 2022Quote: ... Cell nuclei were counterstained with 10 μM 4",6-diamidino-2-phenylindole dihydrochloride (DAPI, Sigma) in TBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were counter stained using 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich, USA) and incubated for 15 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were fixed with 4’,6-diamidino-2-phenylindole (DAPI) mounting media (Sigma-Aldrich). Immunofluorescence-labeled cells were imaged using a fluorescence microscope Eclipse Ci (Nikon) ...