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Citations for Millipore Sigma :
2151 - 2200 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... 300 μM of [3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine sulfonate] Ferrozine (Sigma-Aldrich, Bangalore, India) was added in -Fe media ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by the addition of 60 µL NADPH (N7505, Sigma; 2 mg/3 mL assay buffer). The formation of TNB was monitored kinetically at 412 nm over 5 min in a spectrophotometer (Fluostar Omega ...
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bioRxiv - Cancer Biology 2024Quote: ... Sigma)-treated coverslips were incubated for 3 hours at 37°C with 2 mg/mL laminin (Sigma) in PBS ...
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bioRxiv - Bioengineering 2024Quote: Cell viability was assessed using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide reagent (MTT) assay (Sigma-Aldrich). After the treatment of interest or sham treatment ...
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bioRxiv - Cancer Biology 2024Quote: Tissue culture dishes were coated with 3% poly-2-hydroxyethyl methacrylate (poly HEMA; P3932, Sigma-Aldrich) solution prepared in 95 % absolute ethanol and allowed to polymerize under sterile conditions at room temperature overnight ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.5% (v/v) Triton X-100 supplemented) supplemented with phosphatase inhibitor cocktails 2 and 3 (Sigma) and protease inhibitor cocktail (Roche) ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were washed 3 times with 200mL NM containing 2% L-cystein (Millipore, #52-90-4), pH 7.5 ...
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bioRxiv - Genetics 2021Quote: ... was propagated and maintained by transferring shoot segments of 3-4 cm with 1-2 young leaves to fresh one-half Murashige and Skoog (Beijing, China, Sigma-Aldrich). Plantlets were grown at 23 ℃ under a 16 h light/8 h dark cycle with a light intensity of illumination of 5000 lux provided by cool white fluorescent lamp tubes ...
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bioRxiv - Neuroscience 2021Quote: ... Brain tissue is then washed twice with 2-3 mL sterile-filtered DM+Trypsin Inhibitor (1 mg/mL; Sigma Aldrich, T9253) and incubated in the 3rd wash for 3-5 min at 37° C ...
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bioRxiv - Plant Biology 2021Quote: SDS washed and de-starched dry onion peels were weighed and added to a 2 mL screw – top microcentrifuge tube and 1 mL of 3 M HCl in methanol (Sigma, USA) was added to the walls and incubate for 16 h at 80°C in heating block in a fume hood as per De Ruiter et al ...
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bioRxiv - Biophysics 2021Quote: ... The eluted protein was concentrated to 2-3 mg ml-1 using an Amicon ultracel-100 centrifugal filter unit (EMD Millipore) for amphipol exchange or nanodisc reconstitution ...
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bioRxiv - Microbiology 2022Quote: ... 6-Bromo-4-((dimethylamino)methyl)-5-hydroxy-1-methyl-2-((phenylthio)methyl)-1H-Indole-3-carboxylic acid ethyl ester monohydrochloride (Arbidol) (Sigma Aldrich) was dissolved in ethanol at 10mg/ml and diluted to target concentration in infection media.
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bioRxiv - Microbiology 2022Quote: ... 3-(N-Morpholino)propanesulfonic acid (MOPS) or 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) buffer (all purchased from Sigma-Aldrich, USA). Macrocolonies were excised and resuspended in 2 mL of sterile 1X PBS ...
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bioRxiv - Bioengineering 2022Quote: ... HA-TBA was dissolved in anhydrous DMSO (2 wt%) with a 3:1 M ratio of 5-norbornene-2-carboxylic acid (mixture of endo and exo isomers; Millipore-Sigma) to HA-TBA repeat units ...
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bioRxiv - Microbiology 2022Quote: ... Cleavage of the tag was performed in 2-3 ml with 20 μl 3C protease (1 mg/ml, Sigma-Aldrich, SAE0045) added and incubated over-night at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... were washed two times with PBS and lysed by scraping into 500 μl per plate of lysis buffer (40 mM Tris/HCl pH 7.6, 8 M urea, EDTA-free protease inhibitor complete mini [Roche], and phosphatase inhibitor cocktails 1, 2, and 3 [Sigma-Aldrich] at 1× final concentration according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... were adjusted to 5 x 106 bacteria per 50 μL in PBS then treated with indicated OspC MAbs (1, 2, 3, 10, 20, 30 μg/mL) in the presence or absence of 20% human complement (Sigma-Aldrich) for 16 or 24 h ...
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bioRxiv - Cancer Biology 2024Quote: ... livers were washed 3 times with PBS and incubated 1 hour at room temperature with blocking solution (PBS containing 2 % BSA, Sigma Aldrich) followed by incubation at 4 °C overnight with primary antibody staining (blocking solution containing 1:100 primary antibody) ...
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bioRxiv - Immunology 2021Quote: ... Cloning of the antigen library into the T7 phages was performed following manufacturer’s instructions (T7Select 10-3 cloning kit, catalog no. 70550-3, Merck-Millipore, Burlington, MA, USA).
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bioRxiv - Neuroscience 2020Quote: ... FBS supplementation was decreased to 3% FBS 3 days prior to the addition of 10□M Retinoic Acid (RA; Sigma-Aldrich, R2625). During differentiation ...
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bioRxiv - Neuroscience 2020Quote: Human cofilin1 was amplified by PCR using specific oligonucleotides (forward: 5’-CATATGGCCTCCGGTGTG-3’, reverse: 5’-GGATCCTCACAAAGGCTTGCCCTC-3’) and cloned into the pET-15b vector (Novagen, Millipore, UK). By using site-directed mutagenesis ...
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bioRxiv - Microbiology 2021Quote: Viral stocks of molecular clones HIV-1NL4-3 and HIV-1NL4-3 BaL (AIDS Reagents Program) were generated by transfecting HEK293T cells using PEI transfection reagent (Sigma, Cat: 408727). Pseudotyped HIV-1VSVg-NL4-3 ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was isolated from 27 samples (‘EMEK3’, R bulk and S bulk × 3 treatments/ sampling time × 3 replicates) using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Complementary guide oligonucleotides (forward: 5’-CACCGCAGGATTGAAGACCTTAACA-3’; reverse: 5’-AAACTGTTAAGCTCTTCAATCCTGC-3’) were custom synthesized separately by Sigma-Aldrich (St. Louis, MO, USA), annealed ...
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bioRxiv - Biophysics 2021Quote: ... The primers used for the same were a) A350P forward 5’-gctgcatccggccgcatctcggc-3’ and b) A350P reverse 5’-gccgagatgcggccaaggatgcagc-3’ (Sigma Aldrich, India). Full length A350P was thereafter cloned into an empty pEGFP (Clontech ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the AgNP suspension was washed with deionized (DI) water by ultrafiltration through a membrane with pore size of 3 kDa (Amicon Ultracel 3 KDa Ultrafiltration Discs, Millipore, Billerica, USA) under nitrogen gas pressure (1 bar ...
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bioRxiv - Systems Biology 2022Quote: ... The His6-MBP or His6-MBP-14-3-3 concentrations on the resin were determined by pipetting 3×200 μL of diluted resin (corresponding to 3×15 μL of resin) into a filter plate (Millipore, Burlington, Massachusetts), filtering out the buffer by centrifugation (500g) ...
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bioRxiv - Genetics 2023Quote: ... Single clones were generated and screened for high SpCas9 inducibility after doxycycline treatment (Sigma Aldrich, D9891, 3 µg/ml for 3 days) via Western Blot and flow cytometry after intracellular staining using anti-Flag antibody (Sigma Aldrich ...
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bioRxiv - Synthetic Biology 2023Quote: ... Samples were loaded onto a Supelco™ Discovery™ HS F5-3 (15 cm×2.1 mm, 3-μm) column (Sigma-Aldrich Co.) kept at 30 °C ...
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bioRxiv - Bioengineering 2023Quote: ... A 0.5 M stock solution of inducer was prepared by solubilizing 3-methyl-benzoate (3-MB) (Sigma-Aldrich, St Louis, MO, USA) in 0.5 M NaOH.
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bioRxiv - Developmental Biology 2023Quote: ... oocytes are first isolated from ovaries by short enzymatic digestion (3 mg/ml Collagenase I, Sigma-Aldrich, #C0130; 3 mg/ml Collagenase II, Sigma-Aldrich, #C6885 ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were probed overnight at 4°C with anti-HA antibody (MBL, M180-3) and anti-glyceraldehyde-3-phosphate dehydrogenase antibody (Sigma-Aldrich, G8795) or with anti-dystrophin (Sigma-Aldrich ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Chemical compounds were first identified using the NIST library and later confirmed with co-elution of synthetic 4-methyl-3-heptanone (Pfaltz and Bauer M19160) and 4-methyl-3-heptanol (Sigma Aldrich M48309). Compounds eluting after 30 minutes were excluded from the analysis due to lack of volatility.
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bioRxiv - Developmental Biology 2024Quote: Back skin from E15.5 mouse embryos (3 control and 3 Prmt5 cKO embryos) was dissected and immediately transferred to ice cold DEPC-PBS (Millipore Sigma, D5758). RNA was isolated using the RNeasy Fibrous Mini Kit (Qiagen ...
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bioRxiv - Developmental Biology 2024Quote: The SHMT2 gene was cloned from cDNA of HeLa cells using primers 5’-GCCCATATGGCCATTCGGGCTCAGCAC-3’ (forward) and 5’-GCCCTCGAGATGCTCATCAAAACCAGGCA-3’ (reverse) and subcloned into the pET41a vector (Novagen, WI, USA) at restriction enzyme sites of NdeI and XhoI ...
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bioRxiv - Cell Biology 2021Quote: ... were reared in 10g/l of glucose/200µM of 3BDO (3-Benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-one) (Sigma Aldrich) supplemented in standard fly food ...
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bioRxiv - Biochemistry 2020Quote: ... Day 1 adult animals were mounted on 2% (vol/vol) agar pads and immobilized with 30 mg/mL 2-3-butaneione monoxime (BDM, Sigma) in M9 buffer.
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bioRxiv - Biochemistry 2020Quote: ... animals were mounted on 2% (vol/vol) agar pads and immobilized in 30 mg/mL 2-3-butaneione monoxime (BDM, Sigma) in M9 buffer ...
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bioRxiv - Neuroscience 2021Quote: ... and Pellet Paint Co-precipitant (Merck Millipore #69049-3). Genomic DNA traces were removed with DNA-free DNase Treatment and Removal Reagents (FisherScientific #AM1906 ...
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bioRxiv - Molecular Biology 2020Quote: ... oligonucleotides carrying a 3’ Cy5 fluorophore (purchased from Sigma) were ligated subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated in 3% H2O2 (Sigma-Aldrich, Shanghai, China) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... cells were pretreated with 3 μM FTY720 (Sigma-Aldrich) for 2 h ...
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bioRxiv - Cell Biology 2020Quote: ... with 3 % bovine serum albumin (BSA, Sigma-Aldrich # A7906) for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... ICC blocking solution (3% w/v BSA (Sigma-Aldrich), 3% v/v goat serum (Abcam) ...
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bioRxiv - Immunology 2021Quote: ... in the presence of Monensin (3 µM, Sigma Aldrich) for 4 h ...
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bioRxiv - Genetics 2021Quote: Cell fixation with 3% formaldehyde (Sigma-Aldrich, Cat. F8775) was performed as described in Dauban et al ...
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bioRxiv - Plant Biology 2022Quote: Indole-3-acetic acid (IAA, 10 μM; Sigma-Aldrich) and (+/-)-abscisic acid (ABA ...
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bioRxiv - Neuroscience 2021Quote: ... 3’-diaminobenzadine substrate solution (0.5mg/ml; D5637, Sigma Aldrich) in 0.01% H2O2 in PBS was determined for each antigen ...
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bioRxiv - Neuroscience 2021Quote: ... 20 µl 3% (w/v) λ-Carrageenan (Sigma-Aldrich) in 0.9% sterile NaCl solution (saline ...
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bioRxiv - Neuroscience 2021Quote: ... 20 µl 3% (w/v) λ-Carrageenan (Sigma-Aldrich) in PBS 1X was injected into the mouse left hind paw using a Hamilton syringe ...