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Citations for Millipore Sigma :
2101 - 2150 of 7286 citations for Recombinant Salmon il21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and the protein amounts were determined by BCA assay (Sigma). The proteins were denatured by boiling for 10 min in the DTT-containing sample buffer and separated by 4–20% Mini-PROTEIN TGX gels (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Total protein was visualized using Ponceau S stain (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... The proteins were concentrated using Amicon Ultra Centrifugal Filters (Millipore).
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bioRxiv - Biophysics 2023Quote: ... followed by protein concentration via Amicon Ultra 10 MWCO (Millipore). EPR Spectroscopy of Spin-Labeled Grh1 ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were reduced with 4 mM DTT (Sigma-Aldrich) for 60 min at room temperature and alkylated in the dark using 8 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein content was previously checked with Ponceau (Sigma-Aldrich, #P7170) and membranes (Merck ...
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bioRxiv - Microbiology 2023Quote: ... followed by transfer of proteins to an Immobilon membrane (Millipore) using CAPS buffer (CAPS 10mM ...
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bioRxiv - Neuroscience 2023Quote: ... Total transferred protein was visualized with Ponceau S (Sigma-Aldrich) and incubated overnight in blocking buffer (Intercept Blocking Buffer ...
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bioRxiv - Genetics 2024Quote: ... Protein bands were visualized using enhanced chemiluminescence reagents (Millipore, WBKLS0500).
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bioRxiv - Immunology 2024Quote: ... wells were coated with Protein A from Staphylococcus aureus (Sigma). After 48h ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were purified from the medium (Ex-Cell405, Sigma-Aldrich) 2 days post-infection by directly flowing over Ni-Penta™ Agarose-Base Resin (Marvelgent Biosciences) ...
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bioRxiv - Physiology 2024Quote: Protein concentrations were measured using the Bradford reagent (Sigma #B6916). Samples were added at a 1:200 ratio in a 96-well plate and incubated at room temperature for 5 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were then transferred to PVDF membranes (Millipore, Cat# IPVH00010) at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to a 0.2 μm PVDF membrane (Millipore) and probed with primary antibodies overnight at 4°C ...
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bioRxiv - Systems Biology 2024Quote: ... conjugated to protein G agarose beads (Sigma-Aldrich, catalog # IP04). For IP of phosphopeptides containing the (pS/pT)Q and (pS/pT)P phospho-motifs ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore) and probed with α-GFP-HRP antibody (1:1000 ...
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bioRxiv - Physiology 2023Quote: ... Proteins were detected by chemiluminescence using HRP Substrate (WBKLS0500, Millipore) in a ChemiDoc Touch imaging system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein expression was induced using 0.5 µg/ml doxycycline (Sigma) for 24 h.
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bioRxiv - Molecular Biology 2024Quote: ... protein isolated using RIPA buffer (Pierce) containing protease inhibitors (Sigma) and RNA isolated with TriPure (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein solutions were concentrated using Amicon Ultra centrifugal filter (Millipore) for application to FPLC AKTA pure (GE Heathcare) ...
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bioRxiv - Molecular Biology 2024Quote: ... 40 μl of prewashed protein A beads per sample (Sigma; prewashed in lysis buffer as mentioned in the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... 2015 using a compartment protein extraction kit (Millipore cat: 2145). Triturated brain tissue was thawed on ice for ∼30 minutes before being homogenized in 2 ml/g of Buffer C (HEPES (pH7.9) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein was transferred to an Immobilon PVDF membranes (Millipore Sigma) via wet transfer at 30V for 1 hour at 4 °C in 10% Methanol Tris/Glycine buffer using a Criterion™ Blotter (BioRad) ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were transferred to Immobilon-FL PVDF membranes (Merck-Millipore) and washed twice in TBS buffer followed by incubation in Intercept-TBS Blocking Buffer (Li-COR ...
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bioRxiv - Cell Biology 2024Quote: ... Relative protein loading was visualized using Ponceau S Solution (Sigma).
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bioRxiv - Molecular Biology 2024Quote: ... then after preclearing with protein A–Sepharose beads (Sigma-Aldrich), HY5 antibody (AS12 1867 ...
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bioRxiv - Microbiology 2024Quote: ... Separated proteins were transferred to PVDF membranes (Millipore, Billerica, MA) and probed with appropriate dilutions of primary antibody followed by appropriate dilutions of horseradish peroxidase-conjugated secondary antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... was pre-conjugated to magnetic protein A/G beads (Millipore). Chromatin immunoprecipitation was carried out overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was measured using Bradford Reagent (B6916, Sigma Aldrich).
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were blotted on a PVDF membrane (Merck Millipore, IPVH00010). Membranes were incubated in primary antibodies mouse anti-Vinculin (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Secreted proteins were reduced in 10mM Dithiothreitol (DTT; Sigma-Aldrich) at 20°C for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined using Bradford reagent (Sigma-Aldrich, USA) on a spectrophotometer (Infinite F50 Tecan ...
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bioRxiv - Cancer Biology 2024Quote: ... GST protein was purified using glutathione Sepharose 4B beads (Sigma) by rotating overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were transferred to immobilon NC transfer membranes (HATF00010, Millipore) at 400 mA for 2 h in transfer buffer (25 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein samples (50 µg) were reduced with DTT (Millipore Sigma) at a final concentration of 20 mM at 95°C for 10 minutes and alkylated with iodoacetamide (Millipore Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... and subsequently with protein A agarose beads (16-125, Millipore) for another 4 h at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... was used to plot elution profiles of the protein standard mix Supelco® 69385 Protein Standard Mix 15-600 kDa (Sigma-Aldrich/Merck, Darmstadt, Germany), and the analyzed protein samples.
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bioRxiv - Bioengineering 2023Quote: ... loaded to a polyacrylamide gel (30 μg of total protein/lane) and after gel electrophoresis and protein transfer to polyvinylidene difluoride (PVDF) membranes (Millipore, cat. no. IPVH00010, Burlington, MA). The membranes were blocked with 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Molecular Biology 2022Quote: ... 167 mM NaCl) and precleared for 2 h with 250 μL of previously blocked 50% protein-A and protein-G beads (Sigma P-7786 and P-3296 respectively). Blocking was achieved by incubating the beads with 0.5 mg/ml of Ultrapure BSA and 0.2 mg/mL of salmon sperm DNA for 4 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... For p65 IP the lysates were then incubated with 2 μg of antibody CST ref #6956 or CST ref# 8242) at 4 °C for 2 hrs before adding 20 μL of slurry protein G beads (Sigma-Aldrich Fast Flow Protein G sepharose) for 20 min ...
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bioRxiv - Plant Biology 2019Quote: ... Expressed proteins were detected by immunoblotting with anti-FLAG (Sigma, #F1804), anti-cMyc (Fujifilm Wako ...
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bioRxiv - Cancer Biology 2021Quote: ... Flag-protein complexes were eluted using Flag peptide (Millipore, Cat # F3290) at 150 µg/ml by incubating the beads at room temperature for 30 minutes ...
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bioRxiv - Biophysics 2019Quote: ... Proteins were transferred onto a Immobilon-P polyvinylidene difluoride membrane (Millipore) using a Bio-Rad Criterion Blotter ...
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bioRxiv - Biophysics 2020Quote: ... FN3 proteins were expressed in Rosetta DE3 competent cells (EMD Millipore), lysed by sonication ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred onto an Immobilon-P membrane (PVDF, EMD Millipore) using a Bio-Rad system with a 10% Tris-Glycine and 10% methanol transfer buffer diluted in distilled and deionized water ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were resuspended in 100 mM TEAB buffer (triethylammonium bicarbonate, Sigma) and then digested by trypsin (Pierce MS grade ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Darmstadt, Germany) using a BioRad transfer machine after being run through 10% SDS-polyacrylamide (SDS-PAGE ...
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bioRxiv - Cancer Biology 2021Quote: ... Resolved protein was transferred to PVDF membranes (Millipore, Billerica, MA, USA) using a Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were detected using either Luminata Crescendo Western HRP Substrate (Millipore) or ECL Western Blotting Analysis System (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were then transferred to an Immobilon-P transfer membrane (Millipore) using a Trans-Blot Turbo Transfer system (BIO-RAD) ...