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Citations for Millipore Sigma :
2001 - 2050 of 7286 citations for Recombinant Salmon il21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The resolved proteins were transferred onto charged PVDF membrane (Millipore) using semi-dry blotting apparatus ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were visualized using antibodies that recognize PSTAIRE (P7962, Sigma), V5 (Invitrogen) ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 μM M3814 (DNA-dependent protein kinase inhibitor) (Sigma-Aldrich) was added to the cell media for 72 hr to repress non-homologous end joining DNA repair (Riesenberg et al. ...
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bioRxiv - Neuroscience 2022Quote: ... was pre-cleared with Protein A agarose beads (P3476, Sigma). An ‘input’ sample was set aside and 900 µl of supernatant was incubated overnight with beads cross-linked to CAMK2A primary antibody (10 µg ...
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bioRxiv - Neuroscience 2022Quote: ... total tranferred protein levels were quantified using Ponceau S (Sigma) or the No-Stain Labeling Reagent (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were transferred to Immobilon®-FL PVDF membrane (Millipore). Membranes were blocked in Odyssey® Blocking Buffer (LI-COR) ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-microtubule associated protein-2 (MAP2) (Millipore Sigma AB5622), and mouse anti-TUBB3/β3 tubulin (Novus Biologicals ...
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bioRxiv - Molecular Biology 2023Quote: ... The resolved proteins were electro-transferred to PVDF membranes (Millipore). The proteins of interest were detected with the specific antibodies as indicated in the figure legends and above ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were precipitated by adding 100% trichloroacetic acid (Sigma 76039) to a final concentration of 20% v/v and incubated on ice for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-Glial Fibrillary Acidic Protein GFAP (catalog #G9269; Sigma-Aldrich), anti-PP2A antibody (catalog #SAB4200266 ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were transferred to a PVDF (polyvinylidene difluoride) membrane (Millipore) using Wet transference method (Bio-Rad ...
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bioRxiv - Synthetic Biology 2023Quote: ... Protein was extracted using BugBuster Master Mix (Sigma Aldrich, 71456), mixed 1:1 with 2x laemmli sample buffer (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Protein bands were visualized with ECL-chemiluminescent kit (Millipore, MA). The Abs against LC3B was purchased from Novusbio (Littleton ...
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bioRxiv - Physiology 2022Quote: ... specific proteins were detected with anti-RyR2 (MA3-916, Sigma), anti-CaV1.2 (CACNA1C ...
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bioRxiv - Synthetic Biology 2022Quote: ... and lysed by 20 mL Bugbuster protein extraction buffer (Millipore, 50 mL Bugbuster lysis buffer contains - 49 mL Bugbuster reagent ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... 50 μl of Protein A agarose suspension (Merck Millipore, IP02) was incubated with 1.5 μg of the indicated antibodies ...
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bioRxiv - Microbiology 2022Quote: ... Proteins transferred to PVDF-FL membranes (Millipore, Billerica, MA, USA) were detected with mAb PRC5 at a dilution of 1:5000 and mAb PRC1 at a dilution of 1:2500 (23) ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies against pan-talin protein (1:1000, Sigma-Aldrich) and actin control (1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred at 100 V to PVDF membrane (Millipore) for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted protein was concentrated using a centrifugal filter (Millipore; UFC803024) in storage buffer (20 mM Tris HCl ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were transferred to PVDF membranes (EMD Millipore, Darmstadt, Germany), blocked with 5% bovine serum albumin in TBST (20 mM Tris pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... protein samples were transferred to a Immobilon NC membrane (Millipore) using a Genie Blotter (Idea Scientific) ...
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bioRxiv - Physiology 2023Quote: ... and protein concentration was measured using the BCA Assay (Sigma).
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bioRxiv - Neuroscience 2022Quote: Protein concentrations were determined using the Bradford method (B6916, Sigma), samples were boiled for 5 min in 4x-Laemmli’s sample buffer (1610747 ...
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bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-pro-surfactant protein C (Sigma, Ab3786; 1:2000), rabbit anti-TGFβ2 (Proteintech ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to a PVDF-FL membrane (05317, Millipore) with a current of 2.5 mA/cm2 for 1 h ...
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bioRxiv - Microbiology 2023Quote: Total protein concentration was determined colorimetrically (Bradford Reagent, Sigma-Aldrich). The standard curve was prepared using Bovine Serum Albumin (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... protein expression was induced with 0.5 mM IPTG (Sigma-Aldrich) for 1.5 h ...
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The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone CbioRxiv - Biochemistry 2023Quote: ... Proteins in gels were stained with SYPRO orange (Sigma-Aldrich), and gels were imaged with a Typhoon FLA9500 scanner (GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred at 100 V to PVDF membrane (Millipore) for 1.5 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... 40 μl of prewashed protein A beads per sample (Sigma; equilibrated to lysis buffer as mentioned in the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Protein concentration was determined by Bradford assay (Sigma-Aldrich B6916). Lysates were resolved on a 4-12% Bis-Tris NuPage gel (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... bound proteins were eluted in 2× Laemmli buffer (Sigma-Aldrich), boiled for 5 min at 95°C and separated on a 4-12% NuPAGE Novex Bis-Tris precast gel (Thermo Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted with 10 mg/ml reduced glutathione (Sigma) in 50mM Tris pH 8 ...
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bioRxiv - Neuroscience 2023Quote: ... immunoprecipitated proteins were eluted with 3X FLAG peptide (Sigma F4799). Trem2 was immunoprecipitated with either a Rabbit monoclonal antibody raised against the carboxy-terminus of mouse Trem2 (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to PVDF membranes (EMD Millipore, Darmstadt, Germany) and the membrane was blocked with 5% fat-free milk powder in TBST and incubated for 1 h at room temperature or overnight at 4°C with primary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were measured using BCA assay (Sigma-Aldrich #1003290033). 40–50 μg of cell lysate were loaded in precast SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis ...
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bioRxiv - Physiology 2023Quote: ... Separated proteins were transferred to polyvinylidene difluoride membrane (PVDF, Millipore) using a Trans-Blot Turbo Transfer Pack (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Activated protein was transferred to a PVDF membrane (ED Millipore) using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein loading was visualized with Amido Black Staining Solution (Sigma).
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bioRxiv - Microbiology 2023Quote: ... Proteins were then transferred onto PVDF membrane (Immobilon-FL, Millipore) using a semi-dry transfer apparatus (BioRad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Filtered media was then loaded onto a Protein A (Millipore) column prewashed in ice cold HEPES-buffered saline (HBS ...
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bioRxiv - Cancer Biology 2023Quote: Protein was isolated using RIPA buffer with protease (Sigma Aldrich) and phosphatase cocktail inhibitors (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were transferred to polyvinylidene difluoride membranes (Millipore, IPVH00010). Membranes were blocked in 5% skim milk (Nacalai ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration was determined using Bradford reagent (Sigma Aldrich, USA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were precipitated by adding 5-sulfosalicylic acid (Sigma-Aldrich) to a final concentration of 1% ...
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bioRxiv - Biochemistry 2023Quote: ... and an Atto 647N protein labeling kit (Sigma-Aldrich, 76508) were used ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Protein expression was induced by adding L-arabinose (Sigma-Aldrich) to a final concentration of 0.2% when the cultures reached a OD600 of 0.4 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred onto a Polyvinylidene fluoride (PVDF) membrane (Millipore) and blocked with 5% nonfat dry milk in TBST (20 mM Tris-HCl ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... proteins were transferred onto a PVDF membrane (Merck Millipore #IPFL00010) and blocked with 5% skimmed milk-PBST ...