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Citations for Millipore Sigma :
2101 - 2150 of 6152 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... protein-bound beads were resuspended in 50mM TEAB pH 8 (Sigma-Aldrich, T7408) containing 1 μg trypsin (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and added to HeLa cells with 8 μg/ml polybrene (H9268; Sigma-Aldrich). After cultivating the cells for 24 h ...
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bioRxiv - Immunology 2023Quote: ... transduction was performed using a spinoculation protocol with 8 μg/mL Polybrene (Millipore) for 90 minutes at 1,000 x g at 32°C ...
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bioRxiv - Physiology 2023Quote: ... 8-week-old mice were intraperitoneally injected with (10 mg/kg) AOM (Sigma) (18) ...
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bioRxiv - Cell Biology 2022Quote: ... 50-200 μl lentiviral supernatant and 8 μg/ml polybrene (Millipore-Sigma, USA) were usually added directly to ~70% confluent cells in 2.5 ml culture medium in a 6-well ...
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bioRxiv - Microbiology 2023Quote: ... CaCl2 (1 mL/L of an 8 mg/mL stock; Sigma, Cat. # C7902), Hemin (0.5 mL/L of a 10 mg/mL stock which was prepared in 0.1 N NaOH ...
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bioRxiv - Biophysics 2023Quote: ... pH=8 supplemented with 1% (v/v) mammalian protease inhibitor cocktail (Sigma-Aldrich). Cell membranes were solubilized with 1% (w/v ...
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bioRxiv - Synthetic Biology 2023Quote: ... mixed with 1.5 µL of 8 µg/mL Polybrene (Sigma-Aldrich #TR-1003) and 1.5 mL of fresh cell culture medium ...
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bioRxiv - Cancer Biology 2023Quote: ... DMEM and RPMI media were supplemented with 8% fetal bovine serum (FBS, Sigma), 1 mM sodium pyruvate ...
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bioRxiv - Genetics 2023Quote: ... RBC lysis buffer (8% Ammonium chloride [NH4Cl], 0.8% Sodium bicarbonate [NaHCO3, Sigma-Aldrich, USA] and 0.4% Ethylenediaminetetraacetic acid [EDTA ...
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bioRxiv - Immunology 2023Quote: ... BMDM were plated on a Millicell EZ 8-well chamber slide (Sigma-Aldrich) with 500 μl for a final concentration of 1×105 cells per well and incubated overnight at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... 50-200 μl lentiviral supernatant and 8 μg/ml polybrene (Millipore-Sigma, USA) were usually added directly to ~70% confluent cells in 2.5 ml culture medium in a 6-well ...
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bioRxiv - Immunology 2023Quote: ... Reagents used in this study include: 8 µM Cytochalasin D (Millipore Sigma, #250255), 10 µM Oligomycin A (Selleck Chemicals ...
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bioRxiv - Neuroscience 2023Quote: ... MBEC were plated on collagen-coated 8-well chamber slides (C86024, Millipore Sigma). At ∼90% confluence ...
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bioRxiv - Cell Biology 2023Quote: ... Fibroblasts were infected with fresh viral mixture containing 8 μg/ml polybrene (Sigma) 24 hours after seeding ...
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bioRxiv - Cancer Biology 2023Quote: ... Multiplicity of infection (MOI) = 2 and 8 μg/mL of Polybrene (Sigma-Aldrich) were used in both cases ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Microbiology 2023Quote: ... Transductions were performed in the presence of 8 μg/mL polybrene (Merck Millipore). Virus supernatant was removed at 8 hr post-transduction and cells were maintained in fresh growth medium for 48 hr before undergoing 3 µg/mL puromycin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... 8 mM of the auxin 3-indol-acetic acid (IAA; Sigma-Aldrich, I2886) was added to the arrested cells 1 h prior to generating DSBs.
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bioRxiv - Microbiology 2023Quote: ... fixed with 4 (VACV) or 8 (MPXV) % (v/v) formaldehyde (Sigma-Aldrich, F8775), washed in PBS ...
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bioRxiv - Cell Biology 2023Quote: The female D3T3 cell line was cultured as described 8 in DMEM (Sigma), 10% FBS ...
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bioRxiv - Biophysics 2023Quote: ... inclusion bodies were resuspended in denaturing lysis buffer [8 M Urea (Millipore Sigma), 10 mM Tris (Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... containing non-labeled and labeled lysine and arginine variants: Lys-8 (Sigma, 608041) and Arg-10 (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... and 8 nM protocatechuate 3,4-deoxygenase (PCD; Sigma-Aldrich, St. Louis, MO, USA) to stabilize fluorescence and remove molecular oxygen ...
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bioRxiv - Immunology 2024Quote: ... adult mosquitoes were provided with 8% fructose (Emprove Chemicals, Millipore Sigma, MO, USA) ad libitum ...
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bioRxiv - Neuroscience 2024Quote: ... 8 uL 4-Hydroxy-TEMPO inhibitor (1:200 w/v, Sigma Aldrich, 176141), 8uL TEMED accelerator (10% v/v ...
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bioRxiv - Developmental Biology 2024Quote: ... in the β-galactosidase tissue stain base solution (BG-8-C, Merck-Millipore) with either X-gal (B4252 ...
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bioRxiv - Genomics 2024Quote: ... Cells were transduced by spinfection (1000g) with polybrene (8 µg/mL, Sigma-Alrich) to obtain an infection rate of 10%-20% ...
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bioRxiv - Cell Biology 2024Quote: ... Cell cultures were treated with 0.5 µM epoxomicin (Sigma-Aldrich #134381-21-8) for 6 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 ml of the filtered supernatant supplemented with 8 μg/ml polybrene (Sigma) were used to transduce 2 × 106 cells ...
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bioRxiv - Bioengineering 2024Quote: Macrophage viability was assessed using WST-8 (Cellrix) and Live/Dead (Sigma-Aldrich) staining assays after 24 h of incubation on scaffolds w/ or w/o EF stimulation (250 mV ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Bovine serum albumin (BSA; 98% purity, Millipore Sigma; CAS no. 9048-46-8) was added to diluted CPC solutions at 1 mg/mL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Bovine serum albumin (BSA; 98% purity, Millipore Sigma; CAS no. 9048-46-8) was added at 1 mg/mL to create a final HDB solution in BT ...
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bioRxiv - Bioengineering 2019Quote: ... Milliplex Human Angiogenesis / Growth Factor kit Discovery Assay® and the Human Cytokine Array / Chemokine Array (Millipore, St. Charles, MO, USA)] ...
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bioRxiv - Bioengineering 2021Quote: ... Mature human white-like adipocytes were transfected in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522) to mimic the subcutaneous tissue environment ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 6 times with washing buffer and then incubated with anti-human IgG (Jackson Immuno Research 109-036-088) or anti-human IgA (Sigma A0295) secondary antibody conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 48 hours at 4°C with the following primary antibodies: anti-GBA (1:500; human 8E4; human and rodent G4171, Sigma-Aldrich), anti-human α-synuclein (syn211 ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection for mature human white-like adipocytes was performed in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522). Both HeLa cells and mature human white like adipocytes were transfected with LNPs at a final mRNA concentration of 1.25μg/mL ...
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bioRxiv - Microbiology 2024Quote: ... The human ORF sequence encoding for GSDMD was obtained from the Viral Vector Facility (LUMC) human ORF (cDNA) library from Sigma-Aldrich. The gene ORF was obtained in pDONR223 entry vectors and amplified by PCR with overhang primers to introduce partial sequences of an N-terminal FLAG-tag and C-terminal Myc-tag ...
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bioRxiv - Bioengineering 2024Quote: ... The immortalized Podo/Tert256 human podocyte hTERT cell line (Evercyte) was expanded in flasks coated with 6 µg/cm2 human collagen I (Sigma, C7774) at 37°C in MCDB131 basal media (Gibco ...
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bioRxiv - Pathology 2023Quote: ... adiponectin levels were measured by enzyme-linked immunosorbent assays (Human Chemerin ELISA kit, Biovendor, Brno, Czech Republic; Human Resistin ELISA kit, Millipore Sigma; Human Total Adiponectin/Acrp30 Quantikine ELISA Kit ...
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bioRxiv - Cell Biology 2024Quote: Human umbilical vein endothelial cells (HUVECs) were isolated from human umbilical cords using collagenase type I (Sigma-Aldrich, 400 U/mL) to separate the cell layer surrounding the vein lumen ...
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bioRxiv - Molecular Biology 2021Quote: ... ACC 57), K562 (human, DSMZ Germany ACC 10), and Molm-13 (human, DSMZ Germany ACC 554) were cultured in RPMI1640 (Sigma-Aldrich R8758) supplemented with 10 % heat-inactivated FCS and 1 % penicillin streptomycin mixture ...
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bioRxiv - Biochemistry 2019Quote: ... the GAP-334 region of human p120GAP (residues 715-1047) and the SOScat domain of human SOS1 (residues 564-1049) are cloned into pET15b (Novagen / EMD Biosciences) with an N-terminal polyhistidine (His ...
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bioRxiv - Cancer Biology 2019Quote: ... ECM proteins (collagen I and natural mouse laminin were purchased from Life Technologies, human plasma fibronectin from Millipore, human collagen IV from Sigma-Aldrich, recombinant human tenascin C ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or conventional human monoclonal antibodies was detected by HRP-conjugated rabbit anti-human IgG (Sigma, A8792, 1/2000). After washing 50 μL of TMB substrate (Tetramethylbenzidine ...
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bioRxiv - Immunology 2023Quote: ... Immune mediators in cell culture supernatant following CD3/CD28 bead stimulation were measured using a human premixed CD8+ T Cell Human 17-plex panel (Millipore, Temecula, California). All Luminex assays were analyzed using a MAGPIX Instrument and xPONENT software (Luminex ...
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bioRxiv - Biophysics 2019Quote: ... his-EmGFP-DBNL(1-256) and his-EmGFP-DBNL(1-374) were diluted to 10 nM and 100 pM concentration in PBS (D8537, Sigma-Aldrich). The experiments were done by putting 30 µl of sample on top of a cleaned glass cover-slip ...
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bioRxiv - Molecular Biology 2021Quote: ... After washing Laemmli buffer 1X was added followed by boiling and loading on SDS-PAGE for visualization by western blotting using anti-His (Clonetech 631212) and anti-GST (Sigma G1160) antibodies.
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bioRxiv - Molecular Biology 2020Quote: ... coli cells and large quantities of His6 tagged recombinant Nbs were purified with 1ml of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) following periplasmic expression through osmotic shock ...