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Citations for Millipore Sigma :
2001 - 2050 of 6152 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... 0.1% (v/v) Tween 20) containing 5% (w/v) BSA for 1 hour and then incubated with anti-His (Sigma) or anti-GFP antibody (Clontech ...
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bioRxiv - Microbiology 2022Quote: 20 µL of a 2.5 µg/mL solution of an affinity purified His-tagged RBD in 0.1 M NaHCO3 pH 9.8 was coated onto Nunc® MaxiSorp™ 384-well plates (Sigma) and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The polyclonal anti-Hcp antibody was developed after immunization of six-week-old female BALB/c mice with three subcutaneous injections of the purified His-Hcp protein emulsified in Freund adjuvant (Sigma).
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bioRxiv - Cell Biology 2024Quote: ... The filtered lysate was then incubated for 30 min with 1 ml of equilibrated HIS-Select HF Ni-affinity gel (Sigma) and loaded onto a gravity flow column ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant supplemented with final 10 mM imidazole was incubated with His-Select Nickel Affinity Gel (P6611, Lot No. 110M5166, SIGMA) for 1.5 h at 4 °C with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... The clarified lysate was incubated on a rotator (50 min, 4°C) with 1 mL (packed volume) HIS-Select HF Nickel Affinity Gel (Sigma). The beads were collected in 15 mL Poly-Prep chromatography column (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked using 5% nonfat milk and probed with anti-poly-His primary antibody (cat. no. H1029; Sigma-Aldrich) at 1:3,000 dilution or anti- GST primary antibody (cat ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was clarified by centrifugation at 250,000 g for 30 min and the supernatant was incubated with HIS-Select nickel affinity gel (Sigma-Aldrich) for 1 h at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mouse anti-FLAG M2 monoclonal antibody (F1804) and mouse anti-His-tag monoclonal antibody (D291-3S) were purchased from Sigma□Aldrich (St ...
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bioRxiv - Plant Biology 2023Quote: ... bacteria were harvested by centrifugation and pellets were resuspended in 5 ml of binding buffer (His-bind buffer kit (Millipore), proteins were extracted using a Constant cell disrupter system (Constant Systems ...
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bioRxiv - Biochemistry 2022Quote: ... Elutions from the initial IMAC capture step were directly cleaved with His-tagged HRV 3C protease (produced in-house) prior to concentrating using an Amicon 30 kDa MWCO concentrator (Millipore) and dilution with ion exchange chromatography (IEX ...
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bioRxiv - Immunology 2023Quote: ... 150 mM NaCl) using PD-10 columns (GE) and subjected to overnight cleavage at 4 °C with His-tagged TEV protease (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... Grids were blocked in 2% BSA in TBST for 30 minutes and then moved into a 20 µl drop of anti-His tag primary antibody (Sigma) diluted 1:100 ...
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bioRxiv - Plant Biology 2023Quote: The auto activity of yeast baits on Sc-His due to minimal HIS3 expression was determined by spotting them on Sc-His media containing 0-30 mM 3-Amino-1,2,4-triazole (3-AT) (A8056, Sigma-Aldrich, USA). The minimum concentration of 3-AT that completely inhibits the growth of the bait was considered for the Y1H assay (20mM for pdistal ...
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bioRxiv - Biochemistry 2023Quote: The NP gene from strain A/WSN/33 (H1N1) with a His-tag at its C-terminal was cloned in the pET22 plasmid (Novagen). Single point mutation R416A was introduced through the Quick-Change kit (Stratagene ...
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bioRxiv - Biochemistry 2023Quote: ... A soluble extract was prepared by centrifugation at 14,000 × g for 30 min and applied to Ni-NTA His-bind Resin (Millipore Sigma) equilibrated with binding buffer (50 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Microbiology 2023Quote: ... pET28a-His-DNTTIP1 has been generated by in frame insertion of DNTTIP1 cDNA into BamH1/Xho1 sites of pET28a(+) (Novagen, kindly provided by Dr ...
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bioRxiv - Microbiology 2023Quote: ... 30 min at 4°C) and purified by immobilized metal ion affinity chromatography (IMAC) using a His-Select Nickel Affinity Gel (Sigma) in a fast protein liquid chromatography (FPLC ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by four washes with 200 μl of incubation buffer and incubation with 50 μl of anti-His-HRP antibody (Sigma), diluted 1:2000 in incubation buffer for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: Expression of myrArf1 proteins with C-terminal 6x His tags was accomplished via co-expression with yeast N-myristoyltransferase (yNMT) using the pETDuet-1 vector (Novagen) in E ...
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bioRxiv - Biochemistry 2024Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography ...
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bioRxiv - Biochemistry 2024Quote: ... and the final purified SbSIP was concentrated from the flow-through of a second passage through the His-trap column using an Amicon Ultra Centrifugal Filter (Millipore) with a 30 kDa cutoff ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in PBS with 2% HI-FBS (Hyclone, #SH30910.03) containing 1 µg/mL DAPI (Sigma Aldrich, #D9542) to detect dead cells ...
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bioRxiv - Molecular Biology 2024Quote: Bl6 mice were immunized with 50 µg of the His-tagged LC purified protein in PBS and TiterMax (Sigma-Aldrich) adjuvant (1:10 ratio ...
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bioRxiv - Microbiology 2024Quote: ... In the reciprocal experiment 1 mg of N-terminally his-tagged Saci_1104 was loaded onto a Nickel-NTA (Sigma, Germany) column (column volume of 250 µl) ...
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bioRxiv - Molecular Biology 2019Quote: ... for 8 min at room temperature and glycine (125 mM; G8790-1KG; Sigma) was used to quench cross-linking for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transduced in the presence of 8 µg/ml polybrene (Sigma, H9268) with lentiviral supernatant ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cells were lysed directly in 2x Laemmli buffer containing 8 M urea (Sigma), 3 M thiourea (Sigma) ...
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bioRxiv - Developmental Biology 2020Quote: ... with colored food with Orange G dye (Cat. # 1936-15-8) from Sigma. The larvae/flies were homogenized using 0.05% Tween-20 ...
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bioRxiv - Neuroscience 2021Quote: ... approximately 8 mg of L-α-Phosphatidyl-DL-glycerol sodium salt (Sigma P8318) was dissolved in chloroform ...
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bioRxiv - Cell Biology 2022Quote: Cells were cultured with retrovirus and 8 µg/mL polybrene (H9268; Sigma-Aldrich), and stable transformants were selected with blasticidin (022-18713 ...
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bioRxiv - Cell Biology 2022Quote: ... and added to HeLa cells with 8 μg/mL polybrene (H9268; Sigma-Aldrich). After the cells were cultured for 1 day ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... 8 mL viral supernatant was collected and combined with 4.4 g CsCl (Sigma) in 10 mL PBS ...
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bioRxiv - Cell Biology 2020Quote: ... except that HRP-conjugated goat anti-rat IgG (Sigma, 1-8 µg/ml) was used in place of fluorescent secondary antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... Viral supernatants were transduced onto RPE1 cells with 8 ug/mL Polybrene (Millipore). A clonal cell line was picked based on fluorescence of all 3 biosensors.
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bioRxiv - Cancer Biology 2019Quote: ... Medium containing lentiviral particles and 8 mg/mL polybrene (Sigma-Aldrich, St. Louis) was used to infect NOKs or HTK16 cells ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated for 8 hours in the presence of monensin (2uM, Sigma) and Brefeldin A (5ug/mL ...
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bioRxiv - Bioengineering 2021Quote: ... One liter of PBS (pH = 7.2) contained 8 g of NaCl (Sigma-Aldrich),1.44 g of Na2HPO4 (Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... 8 uL 4-Hydroxy-TEMPO inhibitor (1:200 w/v, Sigma Aldrich, 176141), 8uL TEMED accelerator (10% v/v ...
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bioRxiv - Cancer Biology 2021Quote: Mice (8-12 weeks of age) were injected with tamoxifen (Sigma cat#T5648) dissolved in corn oil ...
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bioRxiv - Neuroscience 2020Quote: ... approximately 8 mg of L-α-Phosphatidyl-DL-glycerol sodium salt (Sigma P8318) was dissolved in chloroform ...
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bioRxiv - Genomics 2021Quote: ... the cells were pre-treated with 8 μg/mL polybrene (Sigma, TR-1003) in the culture media for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... from 8% to 32% buffer B (0.1% formic acid, 80% acetonitrile, Sigma-Aldrich). Electrospray ionization was performed using a 2.1 kV spray voltage and a transfer capillary temperature of 275°C ...
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bioRxiv - Pathology 2022Quote: ... OS media were supplemented with 10 mM β-glycerophosphate (Sigma, 13408-09-8), 0.1 mM L-ascorbic acid (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Samples were diluted at least 1:4 in 8 M urea (Sigma-Aldrich) dissolved in 100 mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... (D)-carvone (>96%; CAS# 2244-16-8; all Sigma-Aldrich, Saint Louis, MO), 2-isopropyl-N,2,3-trimethylbutyramide ...
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bioRxiv - Cell Biology 2022Quote: ... 100 mg.ml-1 insulin (11070-73-8, Sigma-Aldrich, St. Louis, MO, USA), 1% v/v penicillin-streptomycin (30-002-CI ...
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bioRxiv - Cell Biology 2022Quote: ... and added to HeLa cells with 8 μg/mL polybrene (Sigma-Aldrich, H9268). After a day ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were infected with lentivirus supplemented with 8 μg/ml polybrene (Sigma–Aldrich) twice within 48 h and then selected with 1 μg/ml puromycin (Sangon Biotech ...