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Citations for Millipore Sigma :
2051 - 2100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Eluates were neutralized by the addition of 1 M Tris base and buffer exchanged into PBS using 10 kDa MWCO spin columns (Millipore Sigma, UFC801096). Purified proteins were stored at -80 °C in 10% glycerol in PBS.
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bioRxiv - Biochemistry 2024Quote: ... The following primary antibodies were used: mouse anti-β-actin (1:10000, A2066 Sigma-Aldrich), rabbit anti-calnexin (1:1000 ...
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bioRxiv - Biochemistry 2024Quote: ... in PBS with 10 mM MgCl2 and either 1 mM ATP (Sigma Aldrich, A1852-1VL) or 1 mM ATP-γ-thiophosphate (Sigma Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... 13 µl of freshly dissolved 27 mM 5 kDa methoxypolyethylene glycol maleimide (mal-PEG) (Sigma 63187) were added ...
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bioRxiv - Biochemistry 2024Quote: ... Approximately 75,000-100,000 cells were plated on 5 mm diameter coverslips (Word Precision Instruments cat# 502040; coated overnight with 0.1 mg/mL poly-L-lysine hydrobromide [Millipore Sigma cat# P9155], and washed 3x with HBSS) and placed in 96-well plates containing 200 μl of complete neuro DMEM for 4 h ...
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bioRxiv - Biochemistry 2024Quote: ... homogenised in 6 mL of 2:1 (v/v) chloroform:methanol containing 0.01% (w/v) butylated hydroxytoluene (BHT, Sigma-Aldrich) as antioxidant ...
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bioRxiv - Biochemistry 2024Quote: ... EGFP sequence cloned into the pIEx-4 plasmid (Sigma-Aldrich, Saint Louis, MO, USA) served as a negative control for cell line selection.
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bioRxiv - Biochemistry 2024Quote: ... and treated with proteinase K (Sigma-Aldrich) for 5 min ...
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bioRxiv - Biochemistry 2024Quote: ... and potential positive clones were grown overnight in LB media for plasmid production and further purification with GenElute™ Plasmid Miniprep Kit (Sigma-Aldrich). After confirmation of the insert sequence by sequencing (DNA Sequencing Unit ...
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bioRxiv - Biochemistry 2024Quote: ... samples were fixed with 1 mL 4% (v/v) paraformaldehyde with phosphate-buffered saline with Tween20 (PTW) (0.1%, v/v, Tween20/phosphate-buffered saline) (Sigma-Aldrich), and subsequently ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 10% v/v fetal bovine serum (Sigma Aldrich, F7524), 1% l-glutamine (Biological Industries – Sartorius ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were probed with mouse anti-HA antibodies (Sigma-Aldrich, H9658, 1:5000) overnight at 40C.
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Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinitybioRxiv - Biochemistry 2024Quote: ... and secondary anti-mouse IgG antibodies conjugated with horseradish peroxidase (#A9044, Sigma Aldrich). The proteins were visualized using Pierce ECL (#32106 ...
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bioRxiv - Biochemistry 2024Quote: HEK-293 cells were seeded on poly-L-lysine (Sigma Aldrich, P4707) coated cover slips in a 24-well plate and transiently transfected the following day with 50-250 ng DNA ...
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bioRxiv - Biochemistry 2024Quote: ... rabbit anti-HA (Sigma-Aldrich, H6908, 1:750), mouse anti-LAMP1 (DSHB ...
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bioRxiv - Biochemistry 2024Quote: ... stearidonic acid (>99%) and uracil dropout medium were obtained from Sigma-Aldrich (St. Louis, MO, USA). The yeast cultures were maintained at 30 °C and under constant shaking (250 rpm ...
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bioRxiv - Biochemistry 2024Quote: ... and benzonase nuclease (Sigma Aldrich, E1014-25KU) for 30 min at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: Wild-type or hHER2+ EMT-6 cells were harvested with Versene and harvested by centrifugation (500 rcf) and resuspended in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL) for 30 min at 37 °C in the presence of cell-tethered ZHER2-CK2α or a D156A analogue (1 μM) ...
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bioRxiv - Biochemistry 2024Quote: ... MTT reagent (Sigma Aldrich) was dissolved in 1x DPBS (5 mg/ml ...
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bioRxiv - Biochemistry 2024Quote: ... and benzonase nuclease (Sigma Aldrich, E1014-25KU) for 30 min at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: EMT-6 hHER2+ cells were harvested with Versene and harvested by centrifugation (500 rcf) and resuspended in PBS with 10 mM MgCl2 and either 1 mM ATP (Sigma Aldrich, A1852-1VL) or 1 mM N6-phenylethyl-ATP (Biolog ...
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bioRxiv - Biochemistry 2024Quote: EMT-6 hHER2+ cells were harvested with Versene and harvested by centrifugation (500 rcf) and resuspended in PBS with 10 mM MgCl2 and 1 mM ATP-γ-thiophosphate (Sigma Aldrich, A1388-25MG) for 30 min at 37 °C in the presence of cell-tethered ZHER2-CK2α or a D156A analogue (1 μM) ...
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bioRxiv - Biochemistry 2024Quote: EMT-6 hHER2+ cells were harvested with Versene and harvested by centrifugation (500 rcf) and resuspended in PBS with 10 mM MgCl2 and either 1 mM ATP (Sigma Aldrich, A1852-1VL) 30 min at 37 °C in the presence of CK2α fusion proteins (1 μM ...
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bioRxiv - Biochemistry 2024Quote: ... EMT-6 hHER2+ cells were harvested with Versene and harvested by centrifugation (500 rcf) and resuspended in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL) for 30 min at 37 °C in the presence of cell-tethered ZHER2-CK2α or a D156A analogue (1 μM) ...
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bioRxiv - Biochemistry 2024Quote: ... on glass coverslips (Blue Star) coated with 0.2% Gelatin (Porcine, Sigma Aldrich) and were fixed with the fixative solution at the end of the experiment ...
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bioRxiv - Biochemistry 2024Quote: ... Cover slips were washed three times with DPBS and mounted on glass slides with Fluoromount Aqueous Mounting Medium (Sigma-Aldrich #F4680). Slides were dried at RT in the dark overnight before imaging ...
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bioRxiv - Biochemistry 2024Quote: ... permeabilized with permeabilization buffer containing 0.02% (w/v) saponin (Sigma-Aldrich #47036) and 1% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 mM benzamidine] and DNase (1 μg/ml) (Sigma). Protein purification was performed using ÄKTA pure chromatography system at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Each sgRNA (30 ng/ul) was complexed with enhanced specificity (eSP) Cas9 protein (50 ng/ul from Millipore-Sigma (58) and individually validated for causing chromosome breaks in mouse zygotes ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were separated by SDS-polyacrylamide gel electrophoresis (PAGE) electroblotted onto a polyvinylidene difluoride (PVDF) membrane (Millipore, IPFL00010). PVDF membrane was masked with Intercept Blocking Buffer (LI-COR ...
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bioRxiv - Biochemistry 2024Quote: ... PCR reactions were performed with KOD Hot Start DNA Polymerase (Sigma 71086-3) using a Mastercycler X50a (Eppendorf) ...
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bioRxiv - Biochemistry 2024Quote: ... containing 0.01% (w/v) butylated hydroxytoluene (BHT, Sigma Aldrich). All samples were sonicated in an ice-cold water bath sonicator for 20 min prior to monophasic lipid extraction following a method previously reported [29 ...
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bioRxiv - Biochemistry 2024Quote: ... 0.2 mM CaCl2 (Sigma), 1 mM phosphoenolpyruvate (Alfa Aesar) ...
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bioRxiv - Biochemistry 2024Quote: Rosetta 2(DE3)pLysS competent cells (Millipore) were used to recombinantly express all CaMKII variants as previously described (Sloutsky et al. ...
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bioRxiv - Biochemistry 2024Quote: ... Each sgRNA (30 ng/ul) was complexed with enhanced specificity (eSP) Cas9 protein (50 ng/ul from Millipore-Sigma (58) and individually validated for causing chromosome breaks in mouse zygotes ...
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bioRxiv - Biochemistry 2024Quote: ... coil Rosetta (DE3) pLysS (Novagen), and cells were grown in LB media until the cell density reached an OD600=0.6 ...
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bioRxiv - Biochemistry 2024Quote: ... The HsCdc6 elution was concentrated in a 30 K Amicon Ultra-15 concentrator (Millipore) and loaded onto a HiLoad 16/600 Superdex 200 pg column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µl of Benzonase nuclease (Sigma-Aldrich, E1014) was added and the mix incubated (37°C ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µl of Benzonase nuclease (Sigma-Aldrich, E1014) was added and the mix incubated at 37°C with shaking for either 0.5 min or 20 min depending on the experiment ...
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bioRxiv - Biochemistry 2024Quote: ... HsCdt1ΔN peak fractions were pooled and concentrated in a 30 K Amicon Ultra-15 concentrator (Millipore), aliquoted ...
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bioRxiv - Biochemistry 2024Quote: ... 1x 20 cv MCM buffer + 5 mM ATP (Sigma-Aldrich, A2383). For dephosphorylation ...
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bioRxiv - Biochemistry 2024Quote: ... The vector was used to transform chemically competent cells of the Rosetta™ strain of Escherichia coli (Novagen) according to the method described by Inoue et al [311] ...
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bioRxiv - Biochemistry 2024Quote: ... and thrombin (Sigma, final amount 14U) with bacterial LPS (Sigma product code L2630-10MG ...
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bioRxiv - Biochemistry 2024Quote: ... and treated with graphene oxide (Sigma Aldrich) as described previously80 ...
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bioRxiv - Biochemistry 2024Quote: ... The beads were washed with coupling buffer and 2.4 pmol streptavidin (Sigma Aldrich) were added for 15 minutes to block any free DNA ends ...
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bioRxiv - Biochemistry 2024Quote: In vitro microclots were made by mixing commercially obtained fibrinogen (Sigma catalogue number 9001-32-5 ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was cleared by centrifugation (158,000 g, 4°C, 1 h) and incubated with anti-FLAG M2 affinity gel (Sigma-Aldrich, A2220) for 1 h at 4°C with rotation ...
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bioRxiv - Biochemistry 2024Quote: ... Then 1.5 µl 20% Ficoll 400 (Sigma-Aldrich, F2637) was added ...
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bioRxiv - Biochemistry 2024Quote: ... HsCdt1 peak fractions were pooled and concentrated in a 30 K Amicon Ultra-15 concentrator (Millipore), aliquoted ...
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bioRxiv - Biochemistry 2024Quote: ... HsORC peak fractions were pooled and concentrated in 30 K Amicon Ultra-15 concentrators (Millipore), aliquoted ...