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Citations for Millipore Sigma :
2001 - 2050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... followed by HRP-tagged anti-rabbit secondary antibody (1:10,000, Sigma-Aldrich).
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bioRxiv - Biochemistry 2024Quote: ... After reduction with DTT and alkylation with iodoacetamide (Sigma–Aldrich), samples were digested overnight with trypsin (Pierce) ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 (DE3) cells (Novagen, Merck, Darmstadt, Germany). TDP-43 protein was purified to homogeneity by nickel affinity chromatography as described by Vega et al ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 (DE3) Codon plus-RIL cells (Novagen, Merck, Darmstadt, Germany). PDI proteins were purified to homogeneity by nickel affinity chromatography as described by Wang et al ...
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bioRxiv - Biochemistry 2024Quote: ... R-domain phosphorylation sites and interacting residues mutants were generated by site-directed mutagenesis using a Q5-New England Biolabs PCR mutagenesis kit together with primers synthesized from Millipore sigma and verified by sequencing (Elim Biopharmaceuticals ...
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bioRxiv - Biochemistry 2024Quote: ... 0.8 g of solid sodium deoxycholate (Sigma) was added to the cell resuspension and ...
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bioRxiv - Biochemistry 2024Quote: Oligonucleotides were purchased from Sigma Aldrich, IDT or Eurofins and are listed in Table S1 ...
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bioRxiv - Biochemistry 2024Quote: ... Neurons were treated with BV02 (10 µM or 40 µM; #SML0140, Sigma) or Fuscioccin (50 µM ...
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bioRxiv - Biochemistry 2024Quote: ... and 50 μL of pyridine (Sigma-Aldrich #270970) overnight (14–16 h ...
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bioRxiv - Biochemistry 2024Quote: ... and α-tubulin (#T6074, Sigma) antibodies.
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bioRxiv - Biochemistry 2024Quote: ... and incubated with anti-FLAG beads (Sigma-Aldrich #A2220) pre-equilibrated with resuspension buffer without TCEP ...
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bioRxiv - Biochemistry 2024Quote: ... The following antibodies were used: rabbit anti-Znf706 (catalog no. AV3298, Sigma-Aldrich) at 1:1000 dilution and rabbit anti-GAPDH (catalog no ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were incubated with anti-Znf706 rabbit polyclonal primary antibody (1: 200 dilutions; catalog no. AV32987, Sigma-Aldrich) and mouse anti-DNA G-quadruplex structure antibody – clone BG4 (Sigma Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were eluted with 50 µg/mL of 1x FLAG peptide (Sigma-Aldrich #F3290), concentrated using 30 kDa centricons (Millipore #UFC9030) ...
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bioRxiv - Biochemistry 2024Quote: Standards of BMP stereoisomers or enzymatic products were mixed with derivatization agent (R)-(-)-1-(1-naphthyl)ethyl isocyanate (Sigma-Aldrich #220442) in a ratio of 1:10 in 1 mL of dichloromethanol (VWR BDH23373.400 ...
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bioRxiv - Biochemistry 2024Quote: ... Cell fixation was performed with 4% formaldehyde (Sigma-Aldrich, Cat. F8775) as described in Cockram et al ...
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bioRxiv - Biochemistry 2024Quote: ... All reaction products were phenol-chloroform extracted and resolved on silica TLC plates (Millipore). Reaction products were spotted 2.5 cm above the bottom of the TLC plate ...
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bioRxiv - Biochemistry 2024Quote: ... FICZ and indirubin were purchased from Sigma-Aldrich (Saint Louis, MO, USA); JHB3 ...
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bioRxiv - Biochemistry 2024Quote: ... using 6–8 kDa MWCO (Millipore, Novagen) dialysis tubes was carried out at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... The primary antibodies used were: MLKL (MABC60, Millipore); P-MLKL (#ab196436 ...
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bioRxiv - Biochemistry 2024Quote: ... mice were fasted for 6 hours (for GTT) or 5 hours (for ITT) and received an intraperitoneal injection of glucose (1g/kg, Sigma Aldrich) or insulin (0.75 units/kg ...
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bioRxiv - Biochemistry 2024Quote: ... The peak fractions of the ETB-CN-FKBP12 complex were collected and concentrated to 12 mg/ml using a centrifugal filter device (Millipore 50 kDa MW cutoff)
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bioRxiv - Biochemistry 2024Quote: ... pH 8.0) supplemented with 1X Protein Inhibitors Cocktail (SERVA) and a tip of spatula of DNAse I (Sigma Aldrich). Bacterial cells lysis was performed by sonication ...
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bioRxiv - Biochemistry 2024Quote: ... A standard curve was drawn using 4-methylumbelliferone (Sigma-Aldrich #M1381) as standards dissolved in reaction buffer + quenching buffer in ratios used for the assay ...
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bioRxiv - Biochemistry 2024Quote: ... enzymatic products were incubated with 1 μg of PLA2G15 or 5 μg of phospholipase A1 from Aspergillus oryzae (Sigma-Aldrich #L3295) for 15 mins at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... at 1:1000 dilution and rabbit anti-GAPDH (catalog no. G9545, Sigma Aldrich) at 1:2500 dilution ...
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bioRxiv - Biochemistry 2024Quote: ... and mouse anti-DNA G-quadruplex structure antibody – clone BG4 (Sigma Aldrich, MABE917), diluted 500-fold in blocking buffer ...
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bioRxiv - Biochemistry 2024Quote: ... concentrated using 30 kDa centricons (Millipore #UFC9030). Concentrated proteins were buffer exchanged with sodium citrate buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Thioflavin-T (ThioT, 50 μM, Sigma) was added to detect the aggregation ...
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bioRxiv - Biochemistry 2024Quote: ... PLA2G15 used in the reactions was obtained by overexpressing C-terminally FLAG-tagged human PLA2G15 in HEK293FT cells and affinity-isolation using anti-FLAG magnetic beads (Sigma-Aldrich #M8823).
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bioRxiv - Biochemistry 2024Quote: ... Polyethlene glycol (avg. MW 8000) were purchased from Sigma Aldrich, Imidazole from Acros organics and Pierce™ TCEP-HCl from Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was filtered with a 0.22 µm syringe filter (Millipore-sigma) prior to affinity chromatography.
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bioRxiv - Biochemistry 2024Quote: Human embryonic kidney cells (HEK293T) and human fibroblast cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma Aldrich), supplemented with fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2024Quote: ... and 100 μg/mL streptomycin (Sigma Aldrich) at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: BSC1 cells were cultured at 37°C 10% CO2 in DMEM (Sigma) supplemented with 10% FCS and Penicillin / Streptomycin ...
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bioRxiv - Biochemistry 2024Quote: ... Colour was developed by adding 100 µL of 3 mg/mL disodium 4-nitrophenyl phosphate solution (DNPP, Sigma Aldrich) dissolved in 100 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... or 1 mM ATP-γ-thiophosphate (Sigma Aldrich, A1388-25MG) for 30 min at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... and alkaline lignin were purchased from Sigma Aldrich (France). AZCL-xylan (birchwood) ...
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bioRxiv - Biochemistry 2024Quote: Commercial transparent LDPE film obtained from external packaging supplied with 50 mL polypropylene centrifuge tubes (Grenier, Kremsmünster, Austria; Sigma-Aldrich catalog number T2318). The crystallinity of the film was determined to be 43.7 ± 0.1% ...
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bioRxiv - Biochemistry 2024Quote: ... L-DOPA was obtained from Cayman Chemical Company (Ann Arbor, MI, USA) or from Sigma-Aldrich (St. Louis, MO, USA). Butylated hydroxytoluene was purchased from Alfa Aesar (Haverhill ...
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bioRxiv - Biochemistry 2024Quote: ... adhered cells were washed with PBS and treated with cMyc-tagged CK2α or ZHER2-CK2α (1 μM) in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL). Cells were incubated at 37 °C for 30 min ...
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bioRxiv - Biochemistry 2024Quote: Chemicals were obtained from Sigma-Aldrich (St. Louis, MO, USA) unless specified otherwise ...
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bioRxiv - Biochemistry 2024Quote: ... Imidazole was removed from the purified protein by serial buffer exchange in 10 kDa MWCO spin columns (Millipore Sigma, UFC801096). For CD44-Fc constructs ...
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bioRxiv - Biochemistry 2024Quote: ... on which was deposited a 0.22 µM Durapore PVDF membrane (Millipore) that had previously been autoclaved ...
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bioRxiv - Biochemistry 2024Quote: ... and resuspended in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL) in the presence of cMyc-tagged CK2α fusion protein (1 μM) ...
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bioRxiv - Biochemistry 2024Quote: ... and on a commercial CK2 substrate peptide (EMD Millipore, 12-330) under the manufacturer’s recommended conditions ...
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bioRxiv - Biochemistry 2024Quote: Ribonuclease A from bovine pancreas (Fisher, BP2539) and α-lactalbumin from bovine milk (Sigma, L6010) were used for protein studies ...
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bioRxiv - Biochemistry 2024Quote: ... anti-FLAG HRP (1:1000, A8592 Sigma-Aldrich), rabbit anti-GBA1 (1:1000 ...
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bioRxiv - Biochemistry 2024Quote: Two types of PVC powder were purchased from Sigma-Aldrich to be used as model catalase-peroxidase substrates ...
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bioRxiv - Biochemistry 2024Quote: ... Mushroom tyrosinase was supplied by Sigma-Aldrich (catalog number T3824-25KU). L-DOPA was obtained from Cayman Chemical Company (Ann Arbor ...