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Citations for Millipore Sigma :
2001 - 2050 of 7148 citations for Zika Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The transferred proteins were stained with Ponceau S solution (Sigma-Aldrich). The nitrocellulose membrane was soaked (blocked ...
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bioRxiv - Biophysics 2020Quote: ... Protein fractions were pooled and concentrated (10.000 MWCO Amicon; Merck-Millipore) up to 5 mL and analyzed on a HiLoad 26/60 Superdex 200 (GE ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to polyvinylidinefluoride membranes (Millipore Corporation, Billerica, MA, USA), subsequently blocked 5% dry milk in TBST (Tris Buffered Saline with 0.1% Tween 20 ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by addition of Protein-A agarose beads (Sigma-Aldrich #1719408001) for 1 h at 4°C ...
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bioRxiv - Immunology 2020Quote: Total proteins were extracted by RIPA Buffer (R0278, Sigma-Aldrich, USA) containing protease inhibitors (HY-K0010 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by 8% resolving gel containing trichloroethanol (TCE, Sigma) to visualize proteins (Ladner et al. ...
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bioRxiv - Microbiology 2020Quote: ... The protein bands were visualized with a chemiluminescent detection reagent (Millipore).
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bioRxiv - Neuroscience 2021Quote: ... polyclonal goat anti-ribosomal protein L8 (SAB2500882, Sigma-Aldrich; 1:500), monoclonal mouse anti-β-Actin (GTX26276 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein bands were detected using enhanced chemiluminescence (Luminata Crescendo, Merck Millipore). Membranes were re-probed with β-Actin antibody to ensure equal loading.
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bioRxiv - Cell Biology 2020Quote: ... To induce recombinant proteins isopropyl-β-D-thiogalactoside (IPTG, Sigma-Aldrich) was added (0.1 mM ...
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bioRxiv - Physiology 2020Quote: ... Proteins were transferred from the gel onto a PVDF membrane (Millipore) at 30 V for 17 hours at 4°C.
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bioRxiv - Physiology 2021Quote: ... After probing for total protein transferred using Ponceau S (Sigma-Aldrich), membranes were blocked overnight in Odyssey Blocking Buffer (LICOR ...
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bioRxiv - Immunology 2020Quote: ... Digested glycans were cleaned using a PVDF protein-binding membrane (Millipore) and analyzed as described above.
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bioRxiv - Immunology 2020Quote: ... In the experiments using the protein translation inhibitor cycloheximide (Sigma Aldrich), CH12-F3 cells were exposed to low oxygen conditions (1% O2 ...
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bioRxiv - Biochemistry 2021Quote: ... The resolved proteins were transferred to nitrocellulose membranes (0.22 μm, Sigma) and probed with a mouse anti-Strep II monoclonal antibody (1/10,000 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein samples were subsequently concentrated using Amicon 3-kDa concentrators (Millipore). The purity of each protein was detected by SDS-PAGE and western blotting ...
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bioRxiv - Systems Biology 2020Quote: ... Co-immunoprecipitated proteins were detected using anti-Flag antibody (M2 – Sigma), eGFP fusion protein levels monitored using anti-GFP antibody (ab290 – abcam ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, IPVH00010). Then the membranes were blocked with 5% non-fat milk for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... proteins were purified from the infected cells on M2 agarose (Sigma) as previously described (Kim and Roeder ...
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bioRxiv - Cell Biology 2022Quote: ... bound to protein A agarose beads (11719408001, Millipore Sigma, Darmstadt, Germany), as previously done (Carbonneau et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... polyclonal rabbit anti-pro surfactant protein C (1:4000, Millipore, #AB3786), mouse monoclonal acetylated tubulin (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were transferred to a PVDF membrane (Millipore Immobilion-P, IPVH00010) using a wet transfer with freshly made transfer buffer (25 mM Tris ...
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bioRxiv - Bioengineering 2022Quote: Protein concentration was determined with use of the Bradford assay (Sigma) according to manufacturer’s manual if not explicitly mentioned otherwise ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentration was determined by the Bradford assay (Sigma-Aldrich, Europe) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... purified proteins were concentrated using a 10,000 MWCO spin concentrator (Millipore), their concentrations were determined based on their absorption at 280 nm ...
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bioRxiv - Biochemistry 2020Quote: ... The protein containing fractions were concentrated in an Amicon filter (Millipore) with cut off of 10 kDa (GrpE ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated in a 10 kDa Amicon filter (Millipore), and purified on a CHT Ceramic Hydroxyapatite Type II (Biorad ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein was transferred to a 0.45 μm PVDF membrane (Merck Millipore) using BioRad transfer modules in transfer buffer (25 mM Tris ...
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bioRxiv - Bioengineering 2019Quote: ... Protein concentration was measured by the Direct Detect Spectrometer (Merck Millipore).
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bioRxiv - Cell Biology 2019Quote: ... Protein was then transferred to an Immobilon-P PVDF membrane (Millipore), which was probed using antibodies against the following proteins ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were transferred to polyvinylidene difluoride membranes (EMD Millipore, Darmstadt, Germany), blocked with 5% bovine serum albumin in TBST and incubated overnight with primary antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... The protein was then transferred to polyvinylidene difluoride (PVDF) membranes (Millipore), blocked with 5% milk in TBST for 1 hour and incubated with primary antibody overnight at 4°C ...
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bioRxiv - Genetics 2019Quote: ... filtered by using a 0.45 μm low protein binding membrane (Millipore), and then centrifuged at 30,000 rpm and 4°C for 2.5 hrs ...
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bioRxiv - Neuroscience 2019Quote: ... were conjugated with 25uL MagnaChIP protein A/G beads (EMD Millipore). Freshly harvested nuclear pellets from at least 10^6 N2a cells were sheared via Dounce homogenization (15-20 strokes ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were then transferred onto a PVDF membrane (Cat. # IPVH00010; Millipore) using an Electrophoresis Transfer Cell (Bio-Rad) ...
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bioRxiv - Cell Biology 2019Quote: ... Membrane was visualized for total protein with Ponceau S (Sigma-Aldrich) and Western blot was performed using anti-Pmt3 polyclonal serum (gift from Felicity Watts).
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bioRxiv - Cell Biology 2019Quote: ... 80 μl of Magna ChIP protein A+G Magnetic Beads (Millipore) were added to each sample and incubated overnight in a rotating platform at 4 °C ...
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was eluded with 250 mM imidazole (Sigma).
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to PVDF membrane (Millipore, Immobilon-P-Transfer Membrane) using a BioRad wet-transfer system ...
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bioRxiv - Cancer Biology 2019Quote: ... Resolved protein was transferred to PVDF membranes (Millipore, Billerica, MA, USA) using a Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Cancer Biology 2019Quote: ... proteins were detected using either Luminata Crescendo Western HRP Substrate (Millipore) or ECL Western Blotting Analysis System (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitation: 50 μL of Protein A beads suspension (Millipore 16-157) were resuspended in PBS containing 2% BSA and 5 μg of non-immune rabbit polyclonal immunoglobulins (ab37415-5 ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were pre-cleared with PureProteome Protein G magnetic beads (Millipore) at 4°C and 5% of each sample was aliquoted as a percent input control ...
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bioRxiv - Biochemistry 2020Quote: ... The calibration mix (Protein Standard Mix 15 – 600 kDa, Merck Millipore) was composed of the following proteins ...
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bioRxiv - Genetics 2019Quote: ... Bound proteins were eluted using a 3XFLAG peptide (Sigma-Aldrich, F4799) as described in [34] ...
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CHD7 interacts with the nucleosome acidic patch for its efficient activity via its N-terminal regionbioRxiv - Biochemistry 2020Quote: ... The protein was concentrated using Amicon Ultra centrifugal filter devices (Millipore). CHD7ΔN-CRD and CHD7N-CRD_RA were also purified as the wild type full length CHD7.
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bioRxiv - Biochemistry 2020Quote: ... Proteins were co-expressed in Rosetta 2(DE3)pLysS Ecoli (Novagen). Cells were grown at 37°C in 2XYT media to an OD600 of 0.8 ...
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bioRxiv - Immunology 2020Quote: ... Proteins were visualized using Immobilon Forte Western HRP Substrate (Millipore, WBLUF0500).
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bioRxiv - Molecular Biology 2020Quote: ... followed by silver-staining (for proteins, silver nitrate purchased from Sigma) and Western blotting (specific proteins of interest) ...
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bioRxiv - Plant Biology 2021Quote: ... the proteins were transferred to a PVDF membrane (Immobilon-P; Millipore) and examined with protein-specific antibodies ...