Labshake search
Citations for Millipore Sigma :
1901 - 1950 of 7148 citations for Zika Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2019Quote: ... Proteins were electro-transferred to PVDF membranes (Merck Millipore, MA, USA). Immuno-detection was performed with polyclonal antibodies ...
-
bioRxiv - Bioengineering 2021Quote: ... mouse anti-glial fibrillary acidic protein (GFAP)–Cy3 (Sigma, 1:2000) to show astrocyte structure ...
-
bioRxiv - Genetics 2020Quote: ... Proteins were immunoprecipitated using ANTI-FLAG® M2 Affinity Gel (Sigma) for 2 h at 4°C ...
-
bioRxiv - Cancer Biology 2020Quote: ... Proteins expression was detected using an enhanced chemiluminescence kit (EMD Millipore).
-
bioRxiv - Immunology 2021Quote: ... proteins were buffer exchanged in centrifugal concentrators (Amicon Ultra, EMD Millipore) to buffer A plus 300 mM NaCl and 20 mM imidazole ...
-
bioRxiv - Systems Biology 2021Quote: ... Stained proteins were detected using Immobilon Western Chemiluminescent HRP Substrate (Millipore) and image acquisition using a ChemiDoc Imaging system (Bio-rad) ...
-
bioRxiv - Cell Biology 2020Quote: ... EZview™ Red Protein G Affinity Gel was from Sigma Aldrich. Click-iT™ EdU Cell Proliferation Kit for Imaging ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated using Amicon Ultra Centrifugal Filters (Merk Millipore). Selenomethionine derivated PrgL32-208 was purified following the same protocol with the exception that 0.5 mM tris(2-carboxyethyl)-phosphine (TCEP ...
-
bioRxiv - Cell Biology 2021Quote: ... the membrane was blotted with anti-Protein A (1:10,000, Sigma), anti-HA (1:2000 ...
-
bioRxiv - Biochemistry 2021Quote: ... before adding 15 µL of Protein A-Sepharose beads (Sigma-Aldrich) and allowing the incubation to continue for further16 h at 4°C ...
-
bioRxiv - Biophysics 2021Quote: ... the purified protein was concentrated using Amicon Ultra 50 k (Millipore).
-
bioRxiv - Biochemistry 2021Quote: ... or protein A/G agarose beads (EMD Millipore, cat. no. IP05) for 2 hours at room temperature ...
-
bioRxiv - Biochemistry 2021Quote: ... Recombinant proteins were induced in Rosetta™ (DE3) Competent Cells (Novagen) with 0.02% (w/v ...
-
bioRxiv - Biochemistry 2021Quote: ... the N protein was labelled with ATTO 488 dye (Sigma Aldrich) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: Heterologous protein expression was achieved using pET28b(+) (EMD Millipore, Burlington, MA), in which open reading frames for the enzymes were cloned into BamHI/XhoI doubly digested vector ...
-
bioRxiv - Biochemistry 2020Quote: ... Proteins from peak fractions were concentrated using centrifugal ultrafiltration devices (Millipore) to final concentrations of ∼1 mg/ml (RBD-8h proteins) ...
-
bioRxiv - Biochemistry 2020Quote: ... The purified proteins were concentrated by ultracentrifugation (Amicon Ultra-15, Millipore) to ~15-20 mg/ml and stored in 5 mM bis-tris propane–HCl (BTP-HCl) ...
-
bioRxiv - Biochemistry 2020Quote: ... Eluted protein was concentrated using 100-kDa cut off concentrator (Millipore) and further purified by Superose 6 increase (GE Healthcare ...
-
bioRxiv - Neuroscience 2021Quote: ... using myelin basic protein primary antibody (MERCK-Millipore, Massachussets, United States).
-
bioRxiv - Neuroscience 2021Quote: ... mouse anti-Alzheimer Precursor Protein (APP) 22C11 (1:100, MAB348, Millipore), and mouse anti-β-Tub III/Tuj 1 (1:4000 ...
-
bioRxiv - Neuroscience 2020Quote: ... Proteins were reconstituted in sterile DMSO (Millipore-Sigma, Burlington, MA, USA) to stock concentrations of 10 mM ...
-
bioRxiv - Neuroscience 2020Quote: ... Bound DNA and proteins were eluted with 16 mM biotin (Sigma) in water (at pH 7.0 ...
-
bioRxiv - Biophysics 2021Quote: ... Crosslinked protein was concentrated using 100-kDa cut-off concentrator (Millipore) and further purified by Superose 6 increase (GE Healthcare ...
-
bioRxiv - Cancer Biology 2021Quote: ... the protein was transferred to a methanol-activated PVDF membrane (Millipore) at 25 V for 1 hour ...
-
bioRxiv - Cell Biology 2021Quote: ... total protein was detected by staining with Ponceau S (Sigma-Aldrich). Membranes were blocked for 1 hour at room temperature in 5% milk or 5% BSA in Tris-Buffered-Saline-0.1% Tween-20 (TBST) ...
-
bioRxiv - Cell Biology 2021Quote: ... Immunoreactive proteins were visualized by Immobilion western chemiluminescence substrate (Merck-Millipore). Densitometry was carried out by FIJI.
-
bioRxiv - Cell Biology 2021Quote: ... Protein quantification was done using the Bradford colorimetric assay (Sigma B6916). 20µg of proteins were separated run on a 10% SDS-PAGE gel ...
-
bioRxiv - Cancer Biology 2020Quote: ... at 1 ug/ml or control protein Bovine Serum Albumin (Sigma) at 1 ug/ml in PBS were coated onto flat bottom ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Resultant protein concentrations were determined using a Bradford assay (Sigma-Aldrich) and purity of the protein preparation determined by SDS-PAGE followed by silver staining ...
-
bioRxiv - Pathology 2021Quote: ... Proteins from the resulting gels were transferred to PVDF membranes (Millipore), and analyzed by western blotting with relevant antibodies ...
-
bioRxiv - Biophysics 2021Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
-
bioRxiv - Plant Biology 2020Quote: ... Purified protein was concentrated using an Amicon Filter 3K (#UFC500324, Millipore). In order to avoid any interference with downstream procedures ...
-
bioRxiv - Biochemistry 2021Quote: ... Proteins were detected using Immobilon Western Chemiluminescent HRP substrate (Millipore MA) as per the manufacturer’s instructions.
-
bioRxiv - Biochemistry 2020Quote: ... proteins were buffer exchanged in centrifugal concentrators (Amicon Ultra, EMD Millipore) to buffer A plus 300 mM NaCl and 20 mM imidazole ...
-
bioRxiv - Biochemistry 2020Quote: ... The proteins were then transferred to a PVDF membrane (Merck Millipore) using a semi-dry western blot transfer system set to a constant current of 200 mA for 30 min ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein concentrations were determined using the Bradford Reagent (Sigma, Cat# B6916), SDS-PAGE densitometry ...
-
bioRxiv - Biochemistry 2020Quote: ... and the protein inhibitor mixture was from Sigma (catalog no. S8830). Leupeptin ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein bands were transferred to PVDF membranes (Millipore, Cat# PVH00010). After being blocked with 5% non-fat dry milk ...
-
bioRxiv - Biochemistry 2020Quote: ... the protein was concentrated by 50-kDa cut-off Centricon (Millipore) and performed size exclusion chromatography (SEC ...
-
bioRxiv - Plant Biology 2020Quote: ... The immunoprecipitated proteins were incubated with 1 μg MBP (Sigma-Aldrich) in kinase buffer (25 mM Tris-HCl ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Proteins were then transferred onto a PVDF membrane (Milipore Sigma, IPFL00010) using the Biorad Trans-Blot Turbo system in accordance with the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... and proteins were eluted with an excess of FLAG peptide (Sigma) or HA peptide (Sigma ...
-
bioRxiv - Systems Biology 2020Quote: ... The purified proteins were concentrated with Amicon 10K filters (Millipore Sigma) and their concentration assessed using Coomassie and BCA assays (Thermo Fisher Scientific).
-
bioRxiv - Plant Biology 2021Quote: ... proteins were blotted onto a Protan nitrocellulose membrane (Sigma-Aldrich, 10401396). Rabbit anti-RGA polyclonal antibody (Agrisera ...
-
STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... Antibodies were enriched from antiserum using protein A-agarose (Sigma-Aldrich). Affinity purification of TaBGC1-specific antibodies from the antiserum was performed with a HiTrap NHS-Activated HP column (GE Healthcare) ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins were eluted with 50μL of 100μg/mL 3xFLAG peptide (Sigma) in 1x Tris-buffered saline ...
-
bioRxiv - Microbiology 2021Quote: ... cell protein expression was synchronized with cycloheximide (Sigma, 0.1 mg/mL), followed by membrane protein cross-linking with 3,3’-dithiobis(sulfosuccinimidyl propionate ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins were detected using rabbit polyclonal anti-GAPDH (Sigma Aldrich, G9545), mouse monoclonal anti-SARS-CoV-2 Nucleocapsid [1C7] and mouse monoclonal anti-SARS-CoV-2 Spike [2B3E5] protein (a kind gift by Dr ...
-
bioRxiv - Microbiology 2021Quote: ... proteins were transferred onto the PVDF membrane (Immobilon-FL, Merck-Millipore). After the electrotransfer ...
-
bioRxiv - Physiology 2021Quote: ... breast cancer resistance protein (Bcrp-1:100; Merck Millipore, Massachusetts, USA) or Abcg1 (1:200 ...