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Citations for Millipore Sigma :
2001 - 2050 of 10000+ citations for Tick Borne Encephalitis Virus Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... 1 mg/L avidin and 6 mM MgCl2 were added to the supernatant to improve the Strep tag binding efficiency with Strep-Tactin beads (Novagen). Next ...
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bioRxiv - Plant Biology 2021Quote: ... This was achieved using Western blotting detecting the HA-tag (antibody: Anti-HA-Peroxidase, Sigma-Aldrich, St. Louis, USA, 12013819001) and resistance against hygromycin-induced discoloration ...
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bioRxiv - Systems Biology 2020Quote: ... the cDNA was inserted in frame with a 6xHis-GST TEV-cleavable tag into a modified version of pET-30b (Novagen). The NCK2 SH3 shuffled chimeras were constructed using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs ...
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bioRxiv - Plant Biology 2020Quote: ... the coding sequence was excised with BamHI/EcoRI and ligated downstream of the His tag into the multiple cloning site MCS1 of pETDuet-1 (ampicillin resistance; Novagen), yielding Sll0171-pETDuet-1 ...
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bioRxiv - Microbiology 2020Quote: Monoclonal antibody against FLAG tag (ANTI-FLAG M2) and β-Actin were purchased from Sigma (Cat.No. F1804 and A2228, respectively). Monoclonal antibody against human IFITM1 (Cat.No ...
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bioRxiv - Biophysics 2022Quote: ... in this work referred to as Pif1) fused at its N-terminus to a 6-histidine tag was cloned into vector pET28 (Novagen) and transformed into E ...
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bioRxiv - Microbiology 2022Quote: ... and incubated overnight at 4°C with specific primary monoclonal (MAbs) or polyclonal (pAbs) antibodies: anti-HA tag (rabbit pAb; H6908, Sigma), anti-GFP (rabbit pAb ...
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bioRxiv - Bioengineering 2022Quote: ... It was cloned in the expression vector pET30 Ek/lic containing an N-terminal His-tag with the help of the ligation independent Ek/lic system according to the protocol proposed by Novagen, UK ...
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bioRxiv - Biochemistry 2020Quote: ... and a His8 tag was ligated into the corresponding sites in the first multiple cloning site of pCDFDuet-1 (Novagen), yielding plasmid pRHJ001 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked with 5% skimmed milk in Tris Buffered saline-Tween for 1 h before incubation with primary antibody anti-myc tag monoclonal clone 4A6 (Millipore) diluted to final concentration of 200 ng mL−1 in 3% skimmed milk in TBST for 1h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Bound scFv were detected using murine mAb 9E10 which recognizes the C-terminal c-myc tag (1:50 diluted in 2% MPBST) and a goat anti-mouse serum conjugated with horseradish peroxidase (HRP) (A0168, Sigma) (1:42000 dilution in 2% MPBST) ...
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bioRxiv - Biochemistry 2020Quote: ... PCNA-12 co-expression plasmids were constructed by inserting non-tagged PCNA-1 into multiple cloning site 1 and PCNA-2 (without and with a His6-tag) into site 2 of pET-DUET-1 plasmid (Novagen).
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bioRxiv - Microbiology 2022Quote: Recombinant 6XHis-tag GreA or 6XHis-tag GreB were produced by cloning greA and greB genes into NdeI and BamHI sites of the pET14B vector (Novagen) using greA F and greA R or greB F and greB R primers ...
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bioRxiv - Microbiology 2022Quote: ... Anti-FLAG tag or anti-mCherry primary antibodies were used at a concentration of 1:1,250 (Sigma, St. Louis, MO). Then ...
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bioRxiv - Cell Biology 2019Quote: ... carrying two isoleucine residues at the N-terminus and a hexahistidine tag at the C-terminus was expressed in Escherichia coli Rosetta2(DE3)pLysS cells (Novagen) and purified by immobilized metal-affinity chromatography followed by gel-filtration chromatography as described previously (Bishop et al. ...
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bioRxiv - Microbiology 2019Quote: ... This fragment was cloned by ligation independent cloning into the linearized pIEX-10 EK-LIC vector which carries a C-terminal 10xHis tag (Novagen) to generate pIEX-10 ...
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bioRxiv - Molecular Biology 2021Quote: MBL protein levels were determined by SDS-PAGE followed by western blotting with Strep-tag II monoclonal antibodies (at a 1:1000 dilution from 200 μg/ml solution) (Novagen) and immunoglobulin G-alkaline phosphatase conjugates (at a 1:3000 dilution) ...
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bioRxiv - Microbiology 2019Quote: ... and the PCR product was purified and cloned in-frame with a His-tag into the IPTG-inducible expression plasmid pET-15b (Novagen) to create construct pET15b-Pnf ...
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bioRxiv - Bioengineering 2019Quote: For Flag-tag detection membranes were incubated for two hours with the monoclonal anti-Flag M2 antibody from mouse (F3165, Sigma) in a 1:5000 dilution (in TBS-T ...
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bioRxiv - Cell Biology 2021Quote: A Rab11/Ypt32 construct harbouring a GST tag at the N-terminus was expressed recombinantly in Rosetta2 cells (EMD Millipore) and purified using the procedure described previously (21) ...
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bioRxiv - Physiology 2020Quote: ... The presence of LPL in the conditioned media was confirmed through Western blotting using a mouse antibody against the FLAG tag (1:5000; Sigma). LPL activity was assessed through an LPL activity assay (see below).
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bioRxiv - Microbiology 2022Quote: ... the soluble portion of these proteins (excluding the leader peptide and the predicted α1N helix) was fused to non-cleavable N-terminal 6His or Strep II tags and cloned into pET-28b (Novagen). We amplified pilA directly from the S ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tag-free Mpro in the flow-through was concentrated by using Amicon Ultra 15 centrifugal filters (10 kD, Merck Millipore) at 2773 x g ...
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bioRxiv - Microbiology 2022Quote: ... for 5 minutes and then migrated on a 10% SDS polyacrylamide gel and analyzed by Western blot analysis using a monoclonal antibody specific for the FLAG tag (Sigma). Proteins were detected using a goat anti-mouse IgG coupled to IRDye 800CW and LI-COR analysis.
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bioRxiv - Immunology 2022Quote: ... Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was performed using an Apop Tag Plus Peroxidase In Situ Apoptosis Kit (Merck Millipore) following the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2022Quote: ... The proteins were separated by 10 % SDS-PAGE gels and detected by immunoblotting with the first anti-GFP or HA-tag antibodies (Sigma) and the second anti-rabbit IgG-peroxidase antibody (Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mouse anti-FLAG M2 monoclonal antibody (F1804) and mouse anti-His-tag monoclonal antibody (D291-3S) were purchased from Sigma□Aldrich (St ...
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bioRxiv - Microbiology 2022Quote: ... NSm and the entire pcDNA3.1 vector encoding Gn with an N-terminal SS plus HA tag were amplified by PCR using KOD Hot Start DNA polymerase (Novagen), mixed and then concatenated using the NEBuilder HiFi assembly kit according to the manufacturer’s instructions to yield pSnH-OROV-M ...
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bioRxiv - Biochemistry 2023Quote: The NP gene from strain A/WSN/33 (H1N1) with a His-tag at its C-terminal was cloned in the pET22 plasmid (Novagen). Single point mutation R416A was introduced through the Quick-Change kit (Stratagene ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins tagged with the TAP (tandem affinity purification) or HA (hemagglutinin) tag were detected with PAP or an anti-HA antibody (Sigma, Taufkirchen ...
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bioRxiv - Genetics 2023Quote: ... 0.1% Tween-20 and 5% non-fat milk for 1 hour at room temperature and probed for 18 hours at 4 °C with antibodies specific for Flag tag (M2, SIGMA), Myc tag (9E10 ...
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bioRxiv - Molecular Biology 2023Quote: ... with an N-terminal 6×His-mEGFP-TEV tag was inserted using Gibson assembly between the ribosome binding site and T7 terminator of pET-28a(+) (Novagen). Expression vectors for H6-mEGFP-SFPQ ΔN-LCR (SFPQ residues 276-707) ...
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bioRxiv - Biochemistry 2023Quote: ... was fused to a TEV-cleavable N-terminal His6-GCN4 tag and cloned into a pRSF-Duet1 vector (Merck Millipore). The KASH domain sequence of human JAW1/LRMP (amino-acids 515-555 and 531-555 for KASH6 and KASH6 core ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of SNAP-tag fused RBP for each stable cell line was verified by treating with doxycycline (Sigma Aldrich) and SNAP-Cell Oregon Green (NEB).
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bioRxiv - Biochemistry 2023Quote: ... where Cue1(24-203) (pAS153) and Ubc7 (pAS159) were expressed with an N-terminal His6 tag in a pET28B vector (Novagen). Cue1 and Ubc7 were also expressed from a K27-His14-pSUMO vector allowing for the complete removal of affinity and solubility tags55 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Grids were blocked in 2% BSA in TBST for 30 minutes and then moved into a 20 µl drop of anti-His tag primary antibody (Sigma) diluted 1:100 ...
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bioRxiv - Biochemistry 2023Quote: ... the Streptag-purified protein after removal of the C-terminal affinity-GFP-tag was collected and concentrated using 100 kDa MW cut-off spin concentrator (Amicon Merck-Millipore) further purified by size-exclusion chromatography (SEC) ...
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bioRxiv - Developmental Biology 2023Quote: ... 18bp of the reverse complement of the beginning of the DNA tag sequences was added to the 5’ of the reverse primer so that the CRM can be combined with the DNA tag in equal amount using overlap PCR (Xiong et al., 2006) using Expand High Fidelity PLUS PCR (Sigma). The resulting fragment was run on 2% agarose 1x TAE gel ...
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bioRxiv - Biochemistry 2023Quote: ... coli including a polyhistidine tag and thrombin cleavage site at its N-terminus was amplified via PCR and subcloned into pET-30b (Novagen) between the NdeI/ BamHI sites (Vermot et al. ...
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bioRxiv - Microbiology 2023Quote: ... The final dilution of the primary antibody was 1:2,000 for anti-HA tag (Covance) and 1:3000 for anti-β-actin (Millipore-Sigma).and K-Rta (65).
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bioRxiv - Biochemistry 2023Quote: Expression of myrArf1 proteins with C-terminal 6x His tags was accomplished via co-expression with yeast N-myristoyltransferase (yNMT) using the pETDuet-1 vector (Novagen) in E ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmids used to produce ActRII and ActRIIB in bacteria were constructed by inserting the corresponding coding sequence downstream of the N-terminal His6 tag and thrombin cleavage site in pET15b (EMD-Millipore). The plasmid used to produce BMPRII extracellular domain in mammalian cells was constructed by inserting the coding sequence for this downstream of the rat serum albumin signal peptide ...
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bioRxiv - Neuroscience 2020Quote: ... Tau protein ladder (Sigma) was used in some experiments as a control ...
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bioRxiv - Biophysics 2022Quote: ... BLUeye protein ladder (Sigma) was used as a standard ...
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bioRxiv - Biophysics 2022Quote: ... standard proteins BSA (Sigma), Apoferritin (Sigma ...
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bioRxiv - Plant Biology 2022Quote: Protein A-sepharose (Sigma) was washed twice with 5 column volumes of buffer HNTG [20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Protein disulfide isomerase (Sigma) was used in positive control reactions and albumin (Research Organics ...
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bioRxiv - Immunology 2021Quote: ... plus protein A (Sigma).