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Citations for Millipore Sigma :
2001 - 2050 of 10000+ citations for ST2 Human ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... ELISA signal in each elution sample was checked using 1:5000 diluted goat anti-human IgG (Fab specific) HRP-conjugated secondary antibodies (Sigma-Aldrich, A0293-1ML). Elution fractions showing an ELISA signal were pooled and concentrated under vacuum to a volume of ∼1 μL ...
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bioRxiv - Synthetic Biology 2023Quote: ... The CAR T cell product was cultivated in RPMI+10%FBS+1%PS at 37°C with 5% CO2 for experiments and cryopreserved as 10×106 cells/mL in 1 mL 90% heat-inactivated Human AB Serum (Sigma-Aldrich, Cat.#H4522) +10% DMSO in liquid nitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Both mouse and human brain sections were labeled for TH (mouse monoclonal, clone LNC1, 1:2000, Catalog No. MAB318, EMD Millipore, Burlington, MA) and VGLUT2 (rabbit polyclonal ...
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in matched BAL fluid and plasma collected from patients were measured using the multiplexed human cytokine/chemokine magnetic bead kits from Millipore (HCYTMAG-60K-PX41 and HCYP2MAG-62K) according to manufacturer’s protocol (HCYTOMAG-60K Rev ...
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bioRxiv - Immunology 2019Quote: ... IL-17 and CCL17 were determined with ELISA assays according to the manufacturer’s protocols (Sigma, St. Louis, MO, USA).
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bioRxiv - Physiology 2021Quote: ... and adipokine levels were measured via ELISA (adiponectin: Millipore EZ-MADPK, leptin: Millipore EZML-82K, resistin: R&D MRSN00). For HFD experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... The effect of DMAPT on cell proliferation was measured using the Bromodeoxyuridine (BrDU) incorporation-ELISA (Millipore, Cat. No. 2752) as per the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... or IgG2a-HRP conjugated (Southern Biotechnology Associates) and TMB liquid substrate system for ELISA (Sigma-Aldrich, St. Louis, MO). The plates were read in an ELISA plate reader at 405 nM (IgE ...
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bioRxiv - Cancer Biology 2021Quote: ... SMF cells and EMT6 cells were transduced at 50% confluency in 6-well plates with 1 mL of concentrated lentivirus and 6 µg/mL polybrene (Sigma 107689) at 1000xg and 33°C for 2 hours ...
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bioRxiv - Cell Biology 2020Quote: ... 24 well plate) to the adherent osteoblast precursors in presence of DM-medium supplemented with 1 µM PGE2 (Sigma-Aldrich, #P6532) and 10 nM 1,25(OH)2D3 (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... cells were seeded at a density of 1 × 106 cells per well in 12-well plates and stimulated with LPS (100 ng/mL, M1-like - Sigma-Aldrich), IL4 (10 ng/mL ...
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bioRxiv - Genomics 2020Quote: ... Clonal cultures were grown in the dark at 21°C on agar plates made with L1+NPM medium [97,98] and 1% Penicillin–Streptomycin–Neomycin solution (Sigma-Aldrich P4083) to retard bacterial growth.
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bioRxiv - Immunology 2021Quote: ... for 1 hour before being transferred onto Vero E6 cells (ATCC C1008) seeded in a 96-well plate followed by overlay using 1% methylcellulose (Sigma Aldrich). After 24 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... 2 x 106 tumour samples or 1 x 106 spleen samples were plated in 96 well plate (Sigma, Catalogue no. CLS3957). Cell suspensions were pre-incubated with Live/Dead™ fixable Yellow Dead Cell Stain Kit (Invitrogen) ...
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bioRxiv - Biophysics 2020Quote: ... Seeds were surface sterilised with 70% ethanol and grown on vertically oriented plates containing 2.2 g L-1 Murashige and Skoog growth medium (Sigma-Aldrich, pH5.7) supplemented with 1 % sucrose and 0.8 % Bacto agar (BD Biosciences) ...
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bioRxiv - Biochemistry 2020Quote: ... spotting 1 μl of radiolabelled product 1 cm from the bottom of a 20 x 20 cm silica gel TLC plate (Supelco Sigma-Aldrich). TLC was carried out at 35 °C in a humidified chamber with running buffer composed of 30% H2O ...
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bioRxiv - Neuroscience 2020Quote: ... Monolayers of BLECs were treated in 96 well plates and simultaneously incubated with CA-AM (1 μM) (Sigma, St. Louis, MO). Following staining ...
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bioRxiv - Neuroscience 2020Quote: ... Electroporated PBMCs were transferred into 1 well of a 6 well plate seeded with MEFs in PBMC recovery medium (RPMI supplemented with 200uM 1-thioglycerol (Sigma M6145), 1uM Dexamethasone (Sigma D1756) ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were counted and plated at a density of 1 x 106 cells per well of a 6 well plate that was pre-coated with sterile 1% (w/v) gelatin (Sigma-Aldrich). For immunocytochemistry ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed 1-2 days later and the lysates were immunoprecipitated using anti-FLAG M2 affinity resins (10 μl per plate, Sigma Inc.). TNKS antibodies were as described (No ...
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bioRxiv - Microbiology 2020Quote: ... 100 µl of parasites at 0.3% haematocrit was added to a 96 well plate and stained with SYBR Green I (Sigma, 1:5000) in 100 µl (15 minutes ...
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bioRxiv - Systems Biology 2020Quote: ... all transductions were performed in multiple plates with a range of virus volumes and 8 μg mL−1 polybrene (EMD Millipore), but only the pool with the most optimal transduction efficiency was expanded and/or screened.
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bioRxiv - Cell Biology 2021Quote: HEMs or MNT1 cells seeded in a 6-well plate at 50-80% confluency were lysed in 1% Triton X-100 (T8787, Sigma-Aldrich) and centrifuged at 14,000 RPM for 30 min at 4°C to separate solubilized protein from melanin ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 ml of this cell suspension was added per well to a 24-well ultra-low attachment plate (Sigma CLS3473). To test the effect of blebbistatin on aggregate morphology ...
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bioRxiv - Immunology 2022Quote: ... Streptavidin-ALP was added and incubated at room temperature for 1 hour followed by washing and treating plates with substrate solution containing BCIP/NBT (Sigma-Aldrich) until colour development is prominent ...
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bioRxiv - Physiology 2022Quote: ... young adult animals were transferred to 1 mM auxin plates supplemented with 20 mM paraquat (Methyl viologen dichloride hydrate, Sigma-Aldrich). Animals were scored as in lifespan assays but twice a day ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were collected in PBS in 24-well plates and processed for free-floating immunocytochemistry using primary polyclonal antibodies of phosphorylated Tau pSer214 (, rabbit, 1:1000 Sigma, USA) and NOS (Cayman Chemical,1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... were mixed with 100 μl of individual peptide pools in 96-well plates and incubated at 37°C for 1 h after which brefeldin A (Sigma Aldrich) was added and incubation continued for 4-5 h ...
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bioRxiv - Microbiology 2020Quote: ... coli ω7249 strain by electroporation and transformants were selected by tetracycline resistance on a LB plate supplemented with 50 µg mL-1 of diaminopimelate (DAP, Sigma-Aldrich) (30) ...
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bioRxiv - Physiology 2021Quote: ... Vein explants were plated on the dry surface of a cell culture plate coated with 1% Gelatin type B (Sigma-Aldrich). Explants were maintained in RPMI ...
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bioRxiv - Evolutionary Biology 2021Quote: Total bacterial counts were determined by plating out 25 µl of a dilution series ranging from 10−1 to 10−6 on LB-agar plates (Sigma-Aldrich). To identify recipient strains (carrying the dTomato marker ...
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bioRxiv - Biophysics 2021Quote: ... Lipid extracts were resuspended in 60 μl 10mM ammonium acetate in methanol and diluted 1:4 in 96-well plates (Eppendorf twin.tec® 96; Sigma-Aldrich) prior to measurement of PC species ...
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bioRxiv - Physiology 2021Quote: ... elegans were transferred into 24 well plates containing 1 mL M9 Buffer in quadruplicates for each strain and condition (three wells with sodium arsenite (Sigma-Aldrich) and one well M9 as control) ...
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bioRxiv - Genetics 2022Quote: ... cells were grown overnight to exponential phase and serial dilutions (1/10) of cells were spotted on agar plates using a replica plater (Sigma R2383). Plates were photographed after 2–5 days at 30°C.
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bioRxiv - Biochemistry 2019Quote: ... 1 μl of radiolabelled product was spotted 1 cm from the bottom of a 20 x 20 cm silica gel TLC plate (Supelco Sigma-Aldrich). The TLC plate was placed in a sealed glass chamber pre-warmed and humidified at 37 °C and containing 0.5 cm of a running buffer composed of 30% H2O ...
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bioRxiv - Plant Biology 2019Quote: ... wild and transgenic seeds were grown on 0.8% agar-solidified 1/2 MS vertically oriented plates for 7 days with or without specific concentration of Propyzamide (Sigma-Aldrich). To detect the morphology of the cells of 7-day-old seedlings ...
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bioRxiv - Biophysics 2019Quote: ... Of each lipid 10µl at 3mM dissolved in chloroform/methanol (3:1) were deposited in a glass plate coated with indium tin oxide with about 100Ω resistivity (Sigma-Aldrich) and placed 60 minutes in the desiccator for complete solvent evaporation ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were plated overnight at 1 × 106 cells/well in six-well plates coated with poly-L-lysine (Sigma-Aldrich) and transfected the following day using PEI reagent according to the published optimized procedures69 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were passaged to single cell suspension and plated to achieve 65,000 cells per well of a 6-well plate in Nutristem supplemented with 1:2,000 Y-27632 (ROCK inhibitor; Sigma Aldrich, Y0503) [23] ...
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bioRxiv - Neuroscience 2021Quote: ... slices were transferred to a 12-well plate with 1 mL freshly oxygenated ACSF and incubated with either 1mM anisomycin (Sigma #A9789) or vehicle (0.5% DMSO ...
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bioRxiv - Microbiology 2021Quote: ... aeruginosa strains were routinely refreshed from frozen stocks at -80°C and maintained on YPD (1% Bacto yeast extract, 2% Bacto peptone, 2% dextrose, 2% Bacto agar) plates or Pseudomonas isolation agar (Sigma-Aldrich) for in vitro experiments and LB agar (10 g/liter Bacto tryptone ...
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bioRxiv - Genomics 2020Quote: ... transfected in the Lonza 4D nucleofector program ‘CA-137’and seeded onto a Matrigel-covered 6-well plate in StemFlex containing 1 μM H1152 (Millipore, #555550). On the next day ...
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bioRxiv - Bioengineering 2021Quote: ... 1 mg L-1 of the suspended SWNCTs were added to the wells of a 96 well plate to which insulin (Sigma-Aldrich) was added at a final concentration of 33 µg ml-1 ...
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bioRxiv - Immunology 2020Quote: ... 1×105 neutrophils were seeded into a white 96-well plate (Costar 3917) in the presence of the luminol reagent (1 mmol/L; Sigma-Aldrich). Conidia (MOI 1:2 ...
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bioRxiv - Plant Biology 2021Quote: ... The sterilized seeds were sown on ½ Murashige and Skoog (MS) plates with 1% sucrose and 0.6% agar (Millipore-Sigma cat# A1296). After stratification at 4°C for 48 hours ...
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bioRxiv - Plant Biology 2021Quote: ... The sterilized seeds were sown on ½ Murashige and Skoog (MS) plates with 1% sucrose and 0.6% agar (Millipore-Sigma cat# A1296). After stratification at 4°C for 48 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were coated with collagen type I at diluted 1:100 ratio with PBS (solution from rat tail) (Sigma-Aldrich, # C3867) for 1 h at 37°C ...
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Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: PSCdMs and PAMs were plated in triplicate on non-coated 96-well tissue culture plates at 1×105/well in 100 μl Macrophage Maturation medium or RPMI-1640 (Sigma, #R5886), 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed 5 times with PBS/0.5% Tween-20 and 50µl of HRP-anti-mouse-IgA (Sigma-Aldrich, 1:1000) was added to each well ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were grown overnight to exponential phase and five serial dilutions (1/4) of cells were spotted on agar plates using a replica plater (Sigma R2383). Plates were incubated at the relevant temperature ...