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Citations for Millipore Sigma :
2001 - 2050 of 10000+ citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... coated with 10 mg/ml human fibronectin (Sigma). 1 ml of ecotropic retrovirus encoding MSCVneo-HA-ER-Hoxb8 was applied in the presence of 8 mg/ml polybrene and spinoculation was performed at 1000 x g for 60 min at RT ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% AB human serum (Sigma-Aldrich), and plated onto non-treated tissue culture plates (Costar) ...
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bioRxiv - Cell Biology 2023Quote: ... 20ng/mL human fibroblast growth factor (Sigma F5392), B27 (Thermofisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... the secondary antibody anti-human IgG-HRP (Sigma) was added at 1:1000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... Human urine-derived CysC (Millipore Sigma #240896-50UG) was reconstituted in 500 µL of 100mM sodium acetate buffer ...
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Sex and species associated differences in Complement-mediated immunity in Humans and Rhesus macaquesbioRxiv - Immunology 2023Quote: ... Heat-inactivated pooled human sera (Sigma-Aldrich, S1764) and rhesus sera were used as negative controls ...
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bioRxiv - Neuroscience 2024Quote: ... human citrated plasma (Sigma Cat No. P9523-1ML) was added and time to clot formation was recorded.
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bioRxiv - Immunology 2024Quote: ... 800 ml of human Serum (Sigma Aldrich # H4522) were thawed at 37°C after addition of cOmplete™ ...
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bioRxiv - Biophysics 2024Quote: Human Methemoglobin (Ferrohemoglobin) was purchased from Sigma-Aldrich, Missouri ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 5% Human serum (Sigma H4522-100ML), 1% Pen/Strep (Thermofisher 15140-122 ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µg/mL human insulin (Sigma Cat. #91077C), 0.1 nM cholera toxin (Sigma ...
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bioRxiv - Genomics 2024Quote: ... + 5% Human Serum (Sigma-Aldrich catalogue number H4522) + 1% Penicillin-Streptomycin (Gibco catalogue number 15140122 ...
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bioRxiv - Neuroscience 2024Quote: ... 1 μg/ml human insulin solution (Sigma, #I9278), and 1X L-Glutamine (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Complement C6 depleted human serum (C1288, Sigma-Aldrich), diluted to final concentration of 2% with dilution buffer (CH50 assay ...
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bioRxiv - Immunology 2024Quote: ... Non-heat inactivated normal human serum (Sigma-Aldrich) was diluted 1:18 (100% ...
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bioRxiv - Immunology 2024Quote: ... an added positive human IgG Kappa (Sigma-Aldrich) loaded control lane was included (lane P) ...
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bioRxiv - Immunology 2024Quote: ... and 5% human heat-inactivated AB serum (Sigma). The antibodies used for stimulation were anti-human CD3 (clone UCHT1 ...
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bioRxiv - Biochemistry 2024Quote: ... Polyclonal IgM isolated from human serum (Sigma Aldrich) was used as control ...
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bioRxiv - Neuroscience 2024Quote: ... 5 ng/mL human BDNF (Millipore Sigma # SRP3014), 10 µM Rho Kinase Inhibitor (Y-27632 ...
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bioRxiv - Microbiology 2024Quote: ... heat-inactivated 10% human AB serum (Sigma-Aldrich), 1% pen/strep in RPMI1640 (Gibco ...
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bioRxiv - Genomics 2024Quote: ... 2.5 μg/mL human Insulin (Sigma; Cat.No. 19278), 2 μM DAPT (Wako ...
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bioRxiv - Biophysics 2024Quote: ... and 5 IU human chorionic gonadotropin (hCG; Millipore) 48 h later ...
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bioRxiv - Neuroscience 2024Quote: ... 2.5 μg/mL human insulin (Sigma-Aldrich #I9278), 50 μM 2-mercaptoethanol (Gibco #31350010) ...
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bioRxiv - Neuroscience 2024Quote: ... Human Nuclei (Mouse monoclonal, AB1281, Millipore, 1:500), Ki67 (Rabbit polyclonal ...
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bioRxiv - Immunology 2024Quote: ... with 5% FCS + 5% human serum AB (Sigma), penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2024Quote: ... Anti-human-κ+λ-HRP (Millipore, AP502P, AP506P) was added at a 1:4000 dilution in 1% PBSM ...
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bioRxiv - Immunology 2024Quote: ... and 10% pooled human AB serum (Sigma-Aldrich). The blood-media mix was then seeded with ∼150 colony forming units (cfu ...
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bioRxiv - Bioengineering 2024Quote: ... Human IL-8 ELISA Kit (RAB0319, Sigma Aldrich), Human TNF Alpha ELISA Kit (ab181421 ...
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bioRxiv - Bioengineering 2024Quote: ... Human IL-6 ELISA Kit (RAB0306, Sigma Aldrich), Human IL-8 ELISA Kit (RAB0319 ...
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bioRxiv - Biophysics 2024Quote: ... and 0.4 ug/mL human insulin (Sigma 19278). After three days ...
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bioRxiv - Genomics 2024Quote: ... 200µg/mL human holo-transferrin (Sigma-Aldrich, T0665), 1ng/mL recombinant human IL-3 (Peprotech ...
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bioRxiv - Cell Biology 2024Quote: ... 50 ng/ml of human EGF (Sigma, E9644)/ 100 ng/ml Noggin (Cerderlane labs ...
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bioRxiv - Cell Biology 2024Quote: ... followed by blocking with human IgG (Sigma, I4506) at a concentration of 5 μg per 1 x 10⁶ cells on ice for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µg/mL of human insulin (#I9278, Sigma), 100 µM nonessential amino acids (#11140050 ...
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bioRxiv - Developmental Biology 2024Quote: ... 2.5 μg/mL human insulin (Sigma-Aldrich, I9278), 50 μM 2-mercaptoethanol (Gibco ...
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bioRxiv - Immunology 2020Quote: ... Protein extracts were then precleared with protein G beads (EZview™ Red Protein G Affinity Gel, Sigma-Aldrich, Israel), following incubation for 30 min at 4°C ...
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bioRxiv - Biophysics 2020Quote: Codon-optimized and his-tagged (C-terminus) genes for HG-series Kemp eliminases (Supplementary Table 1) cloned into the pET-11a vector (Novagen) via NdeI and BamHI were obtained from Genscript ...
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bioRxiv - Cancer Biology 2021Quote: Anti-Flag (Cat. number: F7425) and anti-His (Cat. number: SAB1306085) antibodies were from Sigma-Aldrich (St. Louis, MS, USA). Anti-phospho-γ-H2AX (Ser139 ...
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bioRxiv - Molecular Biology 2020Quote: Aliquots of 3 μg of GST-LC3B (Viva Bioscience) were incubated alone or with 250 ng of His-STK4 (Millipore) in in vitro kinase reaction buffer (10 mM MgCl2 ...
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bioRxiv - Biochemistry 2020Quote: ... Trx-His(6)-hSLAP2 or Trx-His(6)-Δlinker-hSLAP2 were cloned in frame into multiple cloning site (MCS) 1 of pETDuet-1 (Novagen), with CBL (47-357 ...
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bioRxiv - Plant Biology 2021Quote: ... The cell lysate was centrifuged for 30 minutes at 5,000 x g and the supernatant was recovered and mixed with HIS-Select Nickel Affinity Gel (Sigma Aldrich) for protein purification (manufacturer’s instructions were followed) ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was centrifuged and the resulting supernatant was run through a column filled with N-NTA His-band resin (Millipore). Bound protein was eluted and then dialyzed for overnight in PBS at 4□ ...
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bioRxiv - Microbiology 2021Quote: ... through immunoblotting using anti-His primary antibody raised in mice and monoclonal (goat) anti-mouse secondary HRP-conjugated antibody (Sigma). Further ...
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bioRxiv - Immunology 2020Quote: ... Membrane was then stained with 1 μg/mL of HRP coupled anti-His (monoclonal mouse anti-polyHistidine-Peroxidase, Sigma-Aldrich) in 1% w/V BSA in PBS-T for 1 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... served as blocking buffer and monoclonal mouse-derived anti-HIS antibody (LS-C57341, LsBio) and HRP conjugated goat anti-mouse IgG antibody (AP308P, Sigma) served as primary and secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... bacterial pellet was resuspended in 20 ml of cold His-lysis buffer (50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8.0) with protease inhibitor (Sigma-Aldrich), followed by sonication (10 x 20-second pulses) ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed 4 x 5 min with TBS and the bound SadP was detected with 1:10000 dilution of anti-His primary antibody (Sigma) and 1:20000 dilution of HRP-labelled secondary rabbit anti-mouse antibody (DakoCytomation) ...
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bioRxiv - Immunology 2021Quote: ... Germany) were precoated with murine anti-HIS mAb at a dilution of 1:2,500 (v/v; Sigma-Aldrich, Munich, Germany) at 4°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... were equilibrated for >600 sec in 1x PBS prior to loading with his-tagged NiV prefusion F (25 µg/mL in Blocking Buffer (1x PBS, 1% BSA (Sigma)) for 600 sec ...
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bioRxiv - Biophysics 2022Quote: ... Elutions from the initial IMAC capture step were directly cleaved with His-tagged HRV 3C protease (produced in-house) prior to concentrating using an Amicon 30 kDa MWCO concentrator (Millipore) and dilution with ion exchange chromatography (IEX ...