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Citations for Millipore Sigma :
1851 - 1900 of 10000+ citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Beefy-9 medium was prepared by adding 0.8 mg/mL of recombinant albumin (Sigma #A9731-1G) to Hi-Def B8 ...
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bioRxiv - Immunology 2024Quote: ... 20 ng/mL recombinant mouse GM-CSF (Miltenyi-Biotec) and 0.5 μM ß-estradiol (Sigma-Aldrich) and transduced with ER-HoxB8 retrovirus using Lentiblast Premium (OZ Biosciences).71 After two weeks ...
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bioRxiv - Cell Biology 2020Quote: ... Protein A-Sepharose and Protein G-Sepharose were purchased from Sigma-Alderich ...
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bioRxiv - Plant Biology 2021Quote: ... Protein blots were stained with Ponceau S reversible protein stain (Sigma) to check transfer efficiency and equal protein loading ...
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bioRxiv - Cancer Biology 2021Quote: Protein concentrations were determined using the BCA protein assay (Sigma-Aldrich) using bovine serum albumin as a standard ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was measured by BCA Protein Assay Kit (Sigma-Aldrich) according to manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Thirty microliters of protein G/protein A agarose beads (EMD Millipore) were washed with lysis buffer (containing protein inhibitors ...
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bioRxiv - Cell Biology 2021Quote: ... The protein concentration was determined by a BCA protein assay (Sigma). A total of 50 µg protein extract was mixed with 5× Laemmli loading buffer (50% glycerol ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined using the BCA protein determination reagent (Sigma), and 50 μg were resolved on an SDS/PAGE denaturing gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined using the BCA protein determination reagent (Sigma), and 60 μg were resolved on an SDS/PAGE gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Microbiology 2024Quote: ... then removed the proteins with a 10K MWCO protein concentrator (Millipore) and quantified the resulting HOSCN solution (typically 3.5 to 4 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein quantification was performed with the BCA Protein Assay Kit (Millipore); for each sample ...
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bioRxiv - Cell Biology 2024Quote: Protein quantification using Pierce™ BCA Protein Assay Kit (Sigma-Aldrich) was carried out under manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... purified LC and naïve CD4+ T cells or TRMs were co-cultured in human serum supplemented media (RPMI, Gibco, UK, 10% human serum, Sigma, UK ...
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bioRxiv - Cancer Biology 2020Quote: Human neutrophils were obtained from fresh human peripheral blood from healthy volunteers and were isolated using a Percoll (Sigma-Aldrich) gradient.
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bioRxiv - Microbiology 2020Quote: ... Membranes were probed with polyclonal goat anti-human ACE2 (1:500, AF933, R&D Biosystems) or rabbit anti-human beat-Actin (1:2500, 6L12, Sigma) followed by donkey anti-goat IRdye 680CW or goat anti-rabbit IRdye 800CW (Abcam) ...
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bioRxiv - Physiology 2020Quote: ... For insulin clearance during an insulin challenge, mice were given human insulin (1 U/kg, NovoRapid) or human C-peptide (50 µg/kg, Sigma) by intraperitoneal injection and blood samples were collected by tail vein sampling at 0 ...
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bioRxiv - Microbiology 2020Quote: ... membranes were probed with 1 in 10,000 dilution of human anti-SIC followed by 1 in 50,000 dilution of HRP-conjugated goat anti-human IgG (Sigma-Aldrich). To directly compare detection of SIC fragments by purified human and rabbit anti-SIC antibodies ...
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bioRxiv - Cell Biology 2022Quote: The human alveolar carcinoma epithelial cell line A549 (ATCC, CCL-185) and human colorectal adenocarcinoma cell line HT29 (Sigma Aldrich) were utilized for toxicological evaluation ...
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bioRxiv - Microbiology 2020Quote: ... 15 µl of secondary antibody (anti human Fab-HRP: Jackson ImmunoResearch, cat# 109-035-097; anti human IgG-HRP: Sigma, cat# A6029-1ML ...
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bioRxiv - Microbiology 2023Quote: Primary human macrophages (MΦs) were cultured in macrophage medium (RPMI-1640 GlutaMAX™ (Thermo) supplemented with 4% human AB serum (Sigma), 100 μg/ml penicillin-streptomycin (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... alpha enolase (in-house expressed human) and collagen II (bovine purified, chondrex) and at 0.5 μg/ml hIgG1 to citrullinated fibrinogen (human purifed, Sigma Aldrich).
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bioRxiv - Biophysics 2020Quote: ... biotin binding deficient subunits equipped with a C-terminal His6-tag – “dead” – and functional subunits with C-terminal 3C protease cleaving site followed by Glu6-tag – “alive” – were expressed as inclusion bodies in E.coli BL-21 using the pET expression system (Novagen). Inclusion bodies were dissolved in 6 M Guanidine hydrochloride at 10 mg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... Coding region of H3.1 along with Flag-Tag was amplified by PCR using KOD Hot Start DNA Polymerase (Millipore) and cloned into gateway entry vector pENTR/D-TOPO vector (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... CLP1 contained a C-terminal FLAG-Tag and the sample was purified using ANTI-FLAG M2 Affinity Gel (Sigma), eluted with Pierce 3X FLAG peptide (Thermo ...
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bioRxiv - Microbiology 2020Quote: ... Samples were clarified by centrifugation and incubated with anti-Myc tag antibody conjugated agarose (Millipore, Cat. no. 16-219) for 3h ...
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bioRxiv - Microbiology 2020Quote: ... was used for SDS-PAGE and Western blotting in Tris-buffered saline and 0.2% Tween 20 (TBST) using Strep-Tag II HRP (1:2,500; EMD Millipore) and rabbit anti-vinculin (1:1,000 ...
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bioRxiv - Immunology 2020Quote: ... codon-optimized Bl-Eng2 open reading frame with an upstream enterokinase site and downstream c-Myc and 6X histidine tags was cloned into the SpeI and XhoI sites of the pET43.1b plasmid (Novagen); this forms a fusion protein with an upstream NusA tag ...
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bioRxiv - Immunology 2023Quote: ... Flag-tagged CD45 constructs in DP10.7 Jurkat T cell transductants were stained with 10ug/ml anti-Flag-tag polyclonal antibody (Sigma) for 1hr,
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bioRxiv - Molecular Biology 2022Quote: ... Subsequent GST-tag cleavage was carried out under rotation overnight at 4°C (Thrombin from bovine plasma, Sigma-Aldrich) followed by secondary GST-purification to remove free GST tag (untagged Mcl-1 collected in flowthrough) ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies include: anti-V5 Tag (Invitrogen, ref R96025, 1:500, anti-HA (Sigma-Aldrich, ref H6908, 1:500), anti-Akt (pan ...
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bioRxiv - Cell Biology 2023Quote: ... anti-HA tag (CST, 2367, 1:1000 for WB) and anti-β-actin (Sigma, 2228, 1:2000 for WB) antibodies followed by incubation with HRP-conjugated secondary antibodies (CST ...
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bioRxiv - Molecular Biology 2023Quote: Full length Xenopus laevis Polκ with an N-terminal 6xHis-tag was amplified from pCMV-Sport.ccdb-Polκ (Williams et al., 2012) and cloned into pET28b (Novagen) using primers A and B ...
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bioRxiv - Microbiology 2022Quote: ... incubated 1 hour with primary mouse monoclonal antibody anti-HA tag (G036, Abm Inc.) diluted 1:10,000 and then probed with horseradish peroxidase-labeled anti-mouse immunoglobulin (A9044, Sigma), diluted 1:15,000 ...
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bioRxiv - Cell Biology 2022Quote: ... for Strep-tagII::MLC-4 detection or mouse anti-6xHistidine tag antibody (Millipore clone HIS.H8, 1:2,500, 05-949) for 6xHIS::NMY-2 detection ...
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bioRxiv - Developmental Biology 2022Quote: TAG determination: 60 μl of each sample were mixed with 60 μl of Triglyceride Reagent (Cat. # T2449, Sigma-Aldrich), incubated at 37 °C for 60 min ...
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bioRxiv - Biochemistry 2024Quote: ... Different SRSF3 variants were subcloned in pET28a and expressed as TEV protease-cleavable N-terminal 6xHis-tag fusions in E.coli Rosetta2(DE3) cells (Novagen). E.coli cells were grown in TB media up to an OD600 of ∼ 1.2 at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... anti-p-Histone H2A.X Ser139 (ψH2A.X, mouse monoclonal, 05-636) and pan-ADP-ribose binding reagent (rabbit Fc tag, MABE1016) were obtained from Millipore. anti-DHFR (rabbit polyclonal ...
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bioRxiv - Physiology 2024Quote: ... Lipids were resuspended in 0.1% Triton X-100 and TAGs were determined using a colorimetric triglyceride determination kit (Sigma). True triglycerides were calculated according to a standard curve and the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... Antibodies against H2A.Z (2 μg/mL) (described above for CUT&Tag) or αH3K27me3 (0.5 μg/mL) (Millipore 07-449) antibody was added to diluted lysates and incubated overnight at 4°C on a nutator ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Equal amounts of CRE and Tag DNA were combined using overlap PCR 54,129 using Expand High Fidelity PLUS PCR (Sigma). The product was gel purified using 2% agarose 1X TAE gel and GenElute Gel Extraction Kit (Sigma ...
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bioRxiv - Immunology 2020Quote: ... Protein extracts were then precleared with protein G beads (EZview™ Red Protein G Affinity Gel, Sigma-Aldrich, Israel), following incubation for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and Human specific Ki67 (MAB4383, 1:500, Millipore), followed by secondary antibody Envision+ anti-mouse labeled-polymer (Dako/Agilent) ...
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bioRxiv - Biochemistry 2020Quote: LX-2 human hepatic stellate cells [47] (Millipore) were cultured in DMEM supplemented with 10% fetal bovine serum at 37°C under 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... human plasma-derived (Sigma, Cat. No #: F4883-500MG) were purchased from Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... human plasma-derived (Sigma, Cat. No #: T6884-100UN) and Fibronogen ...
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bioRxiv - Cell Biology 2022Quote: ... Human cells were pretreated with glycine (Millipore, 104201) at a concentration of 50 mM for at least 10 minutes before stimuli was added.
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bioRxiv - Bioengineering 2022Quote: ... human [Leu15]-gastrin I (10 nM; Millipore Sigma), N-acetyl cysteine (500 μM ...
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bioRxiv - Bioengineering 2020Quote: ... human VEGF ELISA kit (RAB0507-1KT, Sigma Aldrich), glutaraldehyde solution (340855 ...
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bioRxiv - Bioengineering 2020Quote: ... sterile fibrinogen from human plasma (Millipore Sigma, 341576) was dissolved in sterile PBS (Corning ...